LOCUS       CP052284                2789 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0224 plasmid pE16KP0224-4,
            complete sequence.
ACCESSION   CP052284
VERSION     CP052284.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609933
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:38:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,501
            CDSs (total)                      :: 5,375
            Genes (coding)                    :: 5,239
            CDSs (with protein)               :: 5,239
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 69 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 23 of 136
            Pseudo Genes (multiple problems)  :: 18 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0224"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0224-4"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2613..2789,1..225))
                     /locus_tag="HJX45_27470"
     CDS             complement(join(2613..2789,1..225))
                     /locus_tag="HJX45_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJJ62193.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(225..416)
                     /locus_tag="HJX45_27475"
     CDS             complement(225..416)
                     /locus_tag="HJX45_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ62189.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            373..796
                     /locus_tag="HJX45_27480"
                     /pseudo=""
     CDS             373..796
                     /locus_tag="HJX45_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            821..952
                     /locus_tag="HJX45_27485"
     CDS             821..952
                     /locus_tag="HJX45_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJJ62190.1"
                     /translation="MHEPPVQSDYHALFGNYRLESNPKRHDKSPLAVAIGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(1243..1662)
                     /locus_tag="HJX45_27490"
     CDS             complement(1243..1662)
                     /locus_tag="HJX45_27490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ62191.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            complement(1659..1856)
                     /locus_tag="HJX45_27495"
     CDS             complement(1659..1856)
                     /locus_tag="HJX45_27495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ62192.1"
                     /translation="MERVLGIYVVGIIVSAGFLFIQEVRNGRRRSYTEEGASMLVGIIC
                     AFLWPLVLIAYFFDKKNGKL"
                     /name="hypothetical protein"
     Typing          640..1149
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.038"
                     /coverage="99.0"
                     /evalue="1.71e-152"
ORIGIN
        1 gtcagcagag gctgtatcac gctgttcaga gacctgtcca gaatggtgtt cacccttcag
       61 ttgttcaaag gggccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattacg
      121 ttcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact
      181 cttacccgtc agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt
      241 aatgcatcca gtctcgcggt cagtgtacag aaaagatgct ccgcatcatc gtggagtttc
      301 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
      361 gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaag
      421 ggcctcagag cgtcagtgct caatgtcaga cagatctctt ttccgcttcc tcgctcactc
      481 agtcgctacg ctcctgccgt tcggctgcgg cgagcggtcg caacgcgcac ataacgacat
      541 agagcagaca gctcggagag ggagaaggca gcacagcggc cgtttttcca taggctccgc
      601 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
      661 ctataaagat accaggcgtt tccccctgat ggctccctcg tgcgctctcc tgttcctgcc
      721 attcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
      781 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
      841 gtccgactac cacgctcttt tcggtaacta tcgtcttgag tccaacccga aaaggcacga
      901 caaatcgcca ttggcggtag ccattggtag agggtgtaca agagattact gatgcaaagt
      961 tcttgaagtt agagccagac tgcggctaaa ctgaaaggac gaatttggtg gcagcggtct
     1021 tgtacaccct gttaccacgg ttcagaagtt ccccaactta ctgaaccttc gaaaaaccac
     1081 ctccccaggt ggttttttcg ttttcagagc aagagattac gacgctcctg aaaggatctc
     1141 aagaagatcc ttttgaactt tggttcattt cttcggctat ccccccatac atgctcgtca
     1201 atctttctat catcaccata gatattcttt gaataaaaga ggttaattgt atgtgaacga
     1261 ggaggaagta ttgtcatcaa tattgattac tctatcgcca cagactctaa gctcatcatt
     1321 tggttgccag tgagcaacat caacaatatt gtcaactaca tgcagagtta ctccgtcgct
     1381 cgtgactaaa agcctaccgt cttctgtcct agctttaaca tacgttgtat cacaactatc
     1441 cgatgaggat tgaacaggaa acccaaaatt aggttgaggg tgctctgatg ccttcaaaac
     1501 tcctcctgtc agacggtcat aaacggtgcc atcaggagtt acagccgaat ttctataagg
     1561 atcatatact ctgtcaggaa taatcgtgtt atctgagtat gctatcggtg atatagccaa
     1621 taaaaaagta atgaccgaga tgaaaaacag acttaacttc ataatttccc atttttttta
     1681 tcaaaaaaat atgcaattaa aactaaaggc cacaaaaacg cacaaattat gccgacaagc
     1741 attgacgcac cttcttcggt gtaacttctg cgcctcccgt tcctaacttc ttggataaac
     1801 aggaaaccgg cactcacaat aataccgaca acataaattc ctaataccct ttccataaaa
     1861 cattccgttc ttatcagatt aaacataaca tcccttgtac gcaccgctaa aacggtctca
     1921 gggcgtttct gaggggaaac cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa
     1981 acgcgcaggc tctgttttta aggcatgcat gagtatgcac gcaagtgcat agcgcgaaaa
     2041 ttcacgccat tcagccagag taagtcctgt ttccggcgtt ctttccatcc gaaaacggtg
     2101 aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaaccggt
     2161 tttcagcacg ttcaggaatc gggttcgtcg acggccggca atcacgttat ggcaagtcgc
     2221 cggacgactc accacacgat tcaccggcga ttcgaatcgt gaattgaatc aagagtcacc
     2281 atgagcaaaa aacacccaaa gtgatgaatt gaatcaagag tcgattcatc ataacaatat
     2341 gataataaag cgtgattcac atatgaatcg tgggcaattc gtcagcgatt cgccatagag
     2401 tcgtccacga cttggatctg cactttgtaa cacatcaccg cacatttatt tttcattaac
     2461 ccattgttgt aaataaggaa tctgcaaaat tttaattttg cgtggggtgt gggtattttt
     2521 agtggtgagg agcgttcaga gatctgacga tttttgggaa ggttcaggaa ctccctggga
     2581 tggccggaga ggaaaactga agcatgacgg ggtcaggatt ttttccagaa tttccaccat
     2641 ggcgccgggg attcagttaa ttttgcctgg tgattttcaa gaaggagcat ggcctgtttc
     2701 aggctgtcaa tgtgcgaatc cttggacaaa agtaaggtgt tcaggtgttc aatctcctgt
     2761 ttcagatgac gaatcacctg ttcactctg
//
