LOCUS       CP052283                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0224 plasmid pE16KP0224-3,
            complete sequence.
ACCESSION   CP052283
VERSION     CP052283.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609933
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:38:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,501
            CDSs (total)                      :: 5,375
            Genes (coding)                    :: 5,239
            CDSs (with protein)               :: 5,239
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 69 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 23 of 136
            Pseudo Genes (multiple problems)  :: 18 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0224"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0224-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(8897..9294,1..1288))
                     /locus_tag="HJX45_27415"
     CDS             complement(join(8897..9294,1..1288))
                     /locus_tag="HJX45_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ62188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            1691..2110
                     /gene="exc2"
                     /locus_tag="HJX45_27420"
     CDS             1691..2110
                     /gene="exc2"
                     /locus_tag="HJX45_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ62178.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            2416..2679
                     /locus_tag="HJX45_27425"
     CDS             2416..2679
                     /locus_tag="HJX45_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ62179.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            2669..2968
                     /locus_tag="HJX45_27430"
     CDS             2669..2968
                     /locus_tag="HJX45_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ62180.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3030..3407)
                     /locus_tag="HJX45_27435"
     CDS             complement(3030..3407)
                     /locus_tag="HJX45_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ62181.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            3476..3655
                     /locus_tag="HJX45_27440"
     CDS             3476..3655
                     /locus_tag="HJX45_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ62182.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3683..4213)
                     /locus_tag="HJX45_27445"
     CDS             complement(3683..4213)
                     /locus_tag="HJX45_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ62183.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4220..4951)
                     /locus_tag="HJX45_27450"
     CDS             complement(4220..4951)
                     /locus_tag="HJX45_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ62184.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(4951..6915)
                     /locus_tag="HJX45_27455"
     CDS             complement(4951..6915)
                     /locus_tag="HJX45_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ62185.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8396..8545)
                     /locus_tag="HJX45_27460"
     CDS             complement(8396..8545)
                     /locus_tag="HJX45_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ62186.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8630..8887)
                     /locus_tag="HJX45_27465"
     CDS             complement(8630..8887)
                     /locus_tag="HJX45_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ62187.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     BGC             1..6288
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7715..8293
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4224..4951)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tggataagga ttcattcatt tcgtttaaag atgtctgggc agcttttaat ttctcattct
       61 gattctgaat atccttatca agatagcgaa cctgatcctg ccacccggag ccatattcat
      121 gaggcgggtt ataagtcaca aaatcatctt tatttgcctc tgcctctttt acctttttat
      181 cggcttcctg aacagcagcc cttgcaccgg ggatcgcctt ctctgtcgat gcaatgcgac
      241 tgtttaaggt cgcaatattt ttatcttcag catccagctc agctttcgct ttatcatact
      301 ctcttttcag cccctcttcc gggtgagcag cgtcccatgc ctgctggcga cgcttttctt
      361 cctccagacg ctgttttacc tgtgccggag taagaacatc cgtcaccgac acatagaccg
      421 gtttctgtcc gctctctttc gggaaacgaa taacggcctc acgggagtta ccaccggccg
      481 taaaaccagc cggacgtgaa tcaccttttt cagcaataat gccttttggc ggggctttcg
      541 cggccggaac acctttaggt acgctcacct gcagagacgg gagacccgga atcgacacac
      601 tgaatacccc cggacgtttt gtcggtttcg catccaccac aggaacactc attggccggc
      661 cggtgacaac cgcaatatgc tgccgctcgt ccttcacaat atccgccaca cgtttagtga
      721 cgctgaccgt cgcctggtcc agcggcaggg agctgaccgg cgtctccgtt accgtgtctg
      781 ccggcaatga cgtcacgatt tttgacatca ttttcggatc atcttttgct atctcagaag
      841 gcagcactcc gtaaatcgca atcccccaca gaccaaactt aaacggacct ttcagggcag
      901 ccagcacatc agcaacagcg gccgacaacg catcgccgga aacggataaa gccagtcctt
      961 cagcccccgg agtggccagg gcaggcagac cgaaggccat cgcggtggcc gaagactgtc
     1021 cgccaccaga gctcccgcta tgattgccgc tgttaccgcc attaccaccg ggtttactcc
     1081 cgccgttacc aaactccccc gggttatagt tatgaatatc cccgttaggt gtatgcgtcg
     1141 taccccagcc cggcccgctg tttggatccg taccaccacc tgatgaaggg ccacctttac
     1201 ctgatgtacc gttcacattc ccactggcct gaccaccatt gtgtcccaga cctgaattac
     1261 ccggacctcg tccgtctccg ccactcatat acttttcctc tttgactttt aaaacaataa
     1321 actaaaataa aatactgtac atataaccac tggttttata tacagtataa aaattacgct
     1381 gccttgtttt tcatgtcaag tctgtaaatt tgcgtttttg tgaaaatgat caaaaaaaca
     1441 gccactcaca ccatgcgcaa tcccctccag atgcaacaaa aaccagcaca aacggcgccg
     1501 ttctccggcc tcagtatgtt ttactgaaat cattatatga gataccagaa agccccctcc
     1561 gggacaggaa aagaaacggg gggtctggtt cgggttgttc ggtcactgaa cacggtcaga
     1621 aactgatgat aagatagcct gcggatatat aactcaatga gaaaaaagaa ttaaaggtaa
     1681 ggagacgctc atgagacgtt ccggcagcaa actgtgtctt atcgccatga taacagcgac
     1741 agcaactctc actgcctgtg ctccaaaggg cagcgtggaa cagcataccc ggcattacgt
     1801 atatgcttca gatgacggtt ttgatcccaa tttttccacc cagaaagctg acacaacacg
     1861 aatgatggtg ccttttttcc ggcagttctg ggatatgggc gcaaaggata aagcgaccgg
     1921 aaaatcacgg agtgatgtgc agcaacgcat tcagcagttt cacagtcagg aatttttaaa
     1981 ctcactccaa ggcacaacac aatttgcggg taccgattac cgcagcaaag acctcacacc
     2041 gaaaaaatca aggctactgg ctgacaccat ttcagctgtt tatctcgatg gctatgaagg
     2101 cagacagtaa gtggatttac cataacgcgt cattacgcgc accgctcaga cgggctcagg
     2161 gcgtttccgg cggctacggg ataaaacagg caacaacccc cccggaaaac tgccggaaac
     2221 gcgcccgata aattttatcc taatgcatgc ctatgcacgc aactgcatgg catatgtttt
     2281 gacgtaaaat ggatgccgta agcccttttt taccgacttt ctgcttcatc agcctgactg
     2341 acacgaccat attctttcag accgccccct gctaaacttc cttcatattg ccgcattaac
     2401 ccgggaggct ccgcaatgag caacactaca cactacgaaa acgccaactt tcttcgggaa
     2461 ctggctgaaa gcctgccccg catactgccg gaaggtggcc cggacaaagc tgcgctgttg
     2521 cagcgactgg ctaacgaaga actggcccag gcagagtatg aagatcaggt tcgcgccaaa
     2581 gtgaccgccg cccgtgctga cacgcgtccg ggtatgacca cggaacaact ccgccagcgc
     2641 ctgcatggcc gttatcagga actgcgcgat gccgtatgac gtggaatgga agcagggagc
     2701 catcgaggat gtaacgacgc tgtttgacta tatcgcggaa aattcatccc tgtgggatgc
     2761 gcagaacgtt accgaccggg ttctcgctgc ggcggacaaa ctggccgact tcccgcgact
     2821 ctatgaagtg gactcacgct atggcgagga cgttcgccgg atcagtctga tgggacagca
     2881 tgtcctctat gatgttgatg accagaccag aaaagtccgg gttctggccg tggtcggcca
     2941 gcgccagaat ccccatattg ttctctaata accggtgacg gggacgcaat tcattgcgtg
     3001 gccgtcgccg gttggacaag cacagcgcgt cagcgttttc cccacggcca ccaccggcgc
     3061 ggtgttccgg ccggggtctc tgctgtctcc ggtttgtgtt ccagcagtct catggactgc
     3121 atttcctgcc gtagcccggc cacctcctcc ctgagtgctt ttatctccgc gatcaacgcg
     3181 tcggaggcca ttttttcagt ctcacccgac agtctcaact cgtcccgctc gtgagtctca
     3241 tcctgtctta actcaccgta cacccggata agctcgctgg tatccaccac ccggccgcca
     3301 tcagcttccg tacggtatga tacgcggcct ttcgccatat cccgatacag ggaacttcgg
     3361 gctttacccg tcagttccgt cgcctgtctc accgtcaccc acgccattgt ctcaccccgc
     3421 tatttttgag acagtatacc tgacaggcgc acaccatgcc cggaaggaaa gaatcatgac
     3481 aaaacaggaa aaaacagccc tcaatatggc taaatttatt aaggagcagt cgctgctatt
     3541 gctcgataaa ctgaatgagc tggatatgga taccccggcc gacatgtgcg agcggctaca
     3601 cgaggatgcg gaaacactct ggcaggtgat gcgggataag ctcggggagg aataacagca
     3661 ccggcatggc cggtgctggc atttattttt tgccgaacgg ccaccaccgg cgggcaggtt
     3721 tgtcctccgg cagttcaggt accggcacgt taaaatagcg gctgttatat tcccgcatca
     3781 gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa
     3841 tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct
     3901 cgatggcgaa ccgctccacc ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt
     3961 tgttccgttc ccattgcgga tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt
     4021 tgagccgctc gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga
     4081 caaaacagaa aacatacgcc ccggtctgcc aggcgagcac caccatatct tccagatgct
     4141 cgctggtcgg catgatgcag cgctcgccgt tccggtcctc cgcgtcttca ataaatacca
     4201 tgtacagcgc cataatccct cactccgttg tcataaattt aaagcacgca aacaaccgtt
     4261 cacgatccag ctctgccgtt ttgcgggcct cgccggtctg tgggttcacg atttcatcca
     4321 ccgacaggat tgcccgctgc accctgacct gcgtcgcccg gtccgggcag atgtaccaga
     4381 tttcatcgat gccgttagct ttgcggttga acagataccc ggacaccact tcctgatacc
     4441 ggcgacggct cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctccg
     4501 ccagggcgtc cggcactttc atgcctttaa gactcagacg gtgcagaatg cgccagccgg
     4561 tggcccctct ggcttccata tcaaggcgca gggattgcac ggccagccgg tgcggcagat
     4621 gtgctgccga cacccttgac ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt
     4681 cgaacgagaa cgccacgcca tgcggcgtca gcccccacac gctgacctga cggccgtcca
     4741 ccggatattt ggtccggacc agcagccctt tcgccaccag gctgtcgagg aagcggtaga
     4801 ccggctgcac cgccgcaatc cccagccgcc gcgcaaccag ctccgccgtg gaacacagct
     4861 cttcccgcaa ccagcgcagg gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg
     4921 cgtggctgac gttgtacagc tccagcgcca tcagtaaatc cccgcaaaat catcatccgc
     4981 tttcggcccg ccgccgtcgg ccgggggccg gaactccgcc aggggatcgg ttttcgccag
     5041 caccgggaaa cgctgcaacg ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc
     5101 catccgggcc acacccagcc cggtcatgac cgcgaacccg tccggcaggt gttgcagggt
     5161 gttcacgtca aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc
     5221 cacgttcccc gcctcccggc tggcacggcg gcgctccacg tccaccctga tttccccggt
     5281 ctgccccgcc gcccactgcg cggactccag gctggccgcc cggtaaaacc agcggatcgg
     5341 cgtgttatcc agtaccgtgg ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc
     5401 gaaatcgccc agcgactggt gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc
     5461 cagtgcgttc agtacatact tcgacagcag atatttaatt tcatcgagca tcacgctgac
     5521 gtgtggccac tcccggaact cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag
     5581 cattttttgc acacggatca ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc
     5641 gccgccgttg ataatccccg ccacatcgat gccactgtcc gtctgcagga cgctcaggtc
     5701 agcgacgttt tccagcctag tgatcaaccc cttcacgtcc tttttcaggg cctccggcag
     5761 cgcgtacgcc gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat
     5821 cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc
     5881 caggttaagc ccggcaatca gcaggttttt cagcgcgtac tggtcgagat cgcggaacag
     5941 attcagctgc gccggtttcc ccggccgcag atccagcagg gtgaagtccg gacagtgcgc
     6001 ccggaagacg tgcggcgccc atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc
     6061 gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg
     6121 caggccgagg atctgaatgt tggtcttgcg ccaggtatca cgcggaacca gcaccggctt
     6181 gccctgctca tccttaccca gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa
     6241 atcggccagc gcattaaccg gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat
     6301 ctctcctgtc cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc
     6361 gtcagcagcc gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat
     6421 cccgacagcc agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag
     6481 gtaataatct gcgtttgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat
     6541 aaacacggcg acaaccagcc cccccagaag ggccagaccg gccgcgccgg tcagccagta
     6601 cgcgtagttc cagacatgtc gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc
     6661 cagcaccgaa aatggcgctg gccgtttatt atcgcgctta tagcgatcgg catactcccg
     6721 tcgctcctta tgactcagcc ccacaaaacg catccgtccg gcccaaagtt cagccagacc
     6781 gaaaaacagg ccgataacta ggaaagcccc ggccagaccg taatactcct ggtacagcca
     6841 gctgacccac ggataaatct gccgggccag ctccggaatg tgccacttcc aggcaagcga
     6901 atccgtatta aacatgttct ctctccttca gacgatggtt tgccggcgtg atgtgcagcc
     6961 cccgctgcac aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg
     7021 attgcgttca gctttcacca cggaggccag cctctttccg ccccatgcgg aaaaggcgca
     7081 gccccggagg aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc
     7141 ggtcttttcc ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg
     7201 caaaatcagg agcagctttg ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg
     7261 gtggtcaggc gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc
     7321 ggacggagcc agaaagaggg ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca
     7381 ggggatatcc ccctgaggtc agccacgccc tcgcggggat gacctgtatt tctccgtaag
     7441 gagaaaaaag caacagagat aggttaacgt aaaatgttca gcgaaaaacc gatatgattc
     7501 acctatcctg gtaatttatt atcagggaat ataaagactt aatcagggga caatatgcca
     7561 catcagcgcg tccggtgtcc gtcttgtagg tgccggggaa attgcgcctc ctcgctcact
     7621 cggtcgctac gctccggccg tgagactgcg gcgggcgctg cggacacatg caaagttacc
     7681 cacagattcc gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag
     7741 gtggcgtttt tccataggct ccgccctcct gactgagttc acataaacag acgctttttc
     7801 ggtgcatctg tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac
     7861 aggacttaaa gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca
     7921 accctgccgt ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt
     7981 ctcatagctc acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg
     8041 taagcaagaa ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga
     8101 gtccaacccg gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc
     8161 gcagaggatt tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct
     8221 acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca
     8281 gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca
     8341 gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact
     8401 gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat
     8461 cccggatata gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa
     8521 gaaaaacaac ctttaccttt ttcatatacc ttccctgtat ttaaaaaccc cggtattcat
     8581 ccctgaacac cggaagtctg tatttcaaat catccttgat aacttttgct taccagttac
     8641 catcccggta aacaaaggat acaaagtaat caaatttact aatatcgatt acatttttaa
     8701 agtgtggctg taatatcgaa acccatgatt tttcaacatc aaaccaacca ttattaatat
     8761 tatcctttaa aggcatcccc agactttcaa tgacagaacc atcatcaccg aagtcttttg
     8821 agtattcacc acctttaaac tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa
     8881 gccccatatt tacctcttaa agatattttt taatgtttcg ttttggatcc ggccctttaa
     8941 cctgcttacc cgtttttgga tcgaatgccc cgaggtgttc gccatcactg gcgcggtacc
     9001 cttcaagctc accgtgctgg gaatcccatt cataaatttt acgcttttta tcaccatacc
     9061 atcgggcccg cttaccacca ccaccttgtt taggggtttt aggtttaccc tctttcaact
     9121 ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt
     9181 tagttccctt acgaggtttt ttcttttcct catttaattt attttctgca tccttcgctt
     9241 ttttctcctt ttgtttccgg ctctccatcg ccccggaaag cgcagcctta tccc
//
