LOCUS       CP052274                4167 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0258 plasmid pE16KP0258-2,
            complete sequence.
ACCESSION   CP052274
VERSION     CP052274.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609936
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:30:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,394
            Genes (coding)                    :: 5,296
            CDSs (with protein)               :: 5,296
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 47 of 98
            Pseudo Genes (incomplete)         :: 44 of 98
            Pseudo Genes (internal stop)      :: 22 of 98
            Pseudo Genes (multiple problems)  :: 13 of 98
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0258"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0258-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(4030..4167,1..978)
                     /locus_tag="HJX48_27550"
     CDS             join(4030..4167,1..978)
                     /locus_tag="HJX48_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QJO54117.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            978..1610
                     /locus_tag="HJX48_27555"
     CDS             978..1610
                     /locus_tag="HJX48_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO54118.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            1976..2218
                     /locus_tag="HJX48_27560"
     CDS             1976..2218
                     /locus_tag="HJX48_27560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO54119.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2564..2965)
                     /locus_tag="HJX48_27565"
     CDS             complement(2564..2965)
                     /locus_tag="HJX48_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO54120.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2965..3156)
                     /locus_tag="HJX48_27570"
     CDS             complement(2965..3156)
                     /locus_tag="HJX48_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO54121.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3113..3547
                     /locus_tag="HJX48_27575"
                     /pseudo=""
     CDS             3113..3547
                     /locus_tag="HJX48_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            3572..3685
                     /locus_tag="HJX48_27580"
                     /pseudo=""
     CDS             3572..3685
                     /locus_tag="HJX48_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          3278..3685
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gatgtaaaaa acccggctgt tttgttaaac attcaagcat tcagaaaaga aatggcagaa
       61 ataaacaaaa acaagccatt ttgggttagt tactttcatg atatggacac atcacaaaag
      121 atgtccatag tcattcgaga tgaacgctct gaagtaacac aaacatatga aacagaaaca
      181 aatcagagat tagaatcaag tttgtctcta aacgttgaca ccgtactatc taaccaaaac
      241 atacctcgtc aaactatcgg ttctcaggcc acagtaaact ctttaaaaat aaatgtgacc
      301 gagacaccca atgcgaaaaa cagggtttcc aataagcttt tctctatgca attgaaagaa
      361 gatggcgtag aaataaaatc cgaaataaaa aacgaaaggg atgggattaa ttataaattt
      421 aagacagcaa caataataac aacatccaga aagataaaca aagaggcaac aattacaaat
      481 gtatctagcc tactaacaca aaaaaggaaa aaagacatac tagaaaactt aaaaaaaata
      541 gatgacagaa ttgtagatat agcaatatct gcgataggta acaataagga aatatattta
      601 gacattggat ttagtgagct aaatgaaata tcaatgcttg gcgagggtat cagtcgagca
      661 ttatctttta tcagctcagt gctggtacag gaaaactcaa ttattcttat tgatgaaatc
      721 gagaacggaa tacattactc agtaattaaa gacataataa aatcattaat ttcatctgca
      781 aaacaaaaca acaatcaaat atttgcaaca actcatagtc aagatgtgat acgagcaata
      841 aacgaaatag atagcaaaaa tgaagatatc gcttatatta gactaggaag agaaaagaac
      901 tccctaaaac caacagccgt acaattcaat atggatgatt tctcctactc agttgagaac
      961 gactgggagg taagataatg aaagacataa ataatagcaa aaagatcatt ttcgttgagg
     1021 gtgatgatga ttttcatttt ctatgcaacc tacttgaaat acaaggcatt accgatgttt
     1081 ttgtcgaaaa aataaatgga aaatcaaaat taaaacaagc acttagagca ttcaaaaaca
     1141 ttgattcttt cgatgaaaaa gaaagtattt ttattatatt agatgctgac acatcatttt
     1201 ctgacacaga aagatcaata attgccacat tgcgagaact acaaatctcc tcccccagct
     1261 cgcataacga gatagctatg aatggcaata caaaaatttc tttctttata atgccaggaa
     1321 aagacaagca aggtgccatt gaagatctga taattgaaca cgcaagcact cgagcgttat
     1381 atgaatacat aatagaattt ttcgaaaaaa ttaaaaataa taaagatgac atctcagcga
     1441 gtgacaaaga gtacacttat ccaacaaacg aaaaaaaagc taaggtacaa gtctatctta
     1501 gctgcaatag agaatccgac tcaagaatag gaatatccat gaaaaatgga ataataaaca
     1561 cagcagacaa ttgctttgat gaaataaaag attttatttc aaaaatataa atccaaatca
     1621 agccagccct tcacgggctg gcttatcacc agggtgttat taaacgccgg ataacgtata
     1681 gaaagcatca tacactttag ttatcagcaa tctcaaacgt cctaaagaca tcaccagatg
     1741 ccaaaacaat gattgtgcag atctaatgta aaagacccag cacaagatgc tcccttaagt
     1801 tatattctct ctattcactt aaaacaattt aacataaaat cataattgta cgcaccgcta
     1861 aaacggactc aggacgtttc tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa
     1921 acccgccaga aacgcgcagg ctctgttttt acggcatgca tgagtatgcg cgcaagtgca
     1981 tagcgcggaa attcacacca ttcagccagg ggaagtcctg tttccggcgt tcttctcctc
     2041 cgaaaacggt gaacggggac gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc
     2101 ggaaaaccgg ttttcagcac cttcaggaat cgggttcgtc gacggccggc aatcacgtta
     2161 tggcaagtcg ccggacgact caccacacga ttcaccggcg attcgaatcg tgaattgaat
     2221 caagagtcac catgagcaaa aaacacccaa agtgatgaat tgaatcaaga gtcgattcat
     2281 cataacaata tgataataaa gcgtgattca catatgaatc gtgggcaatt cgtcagcgat
     2341 tcgccataga gtcgtccacg acttggatct gcactttgta acacatcacc gcacatttat
     2401 ttttcattaa cccattgttg taaataagga atctgcaaaa ttttaatttt gcgtggggtg
     2461 tgggtatttt tagtggtgag gagcgttcag agatctgacg atttttggga aggttcagga
     2521 actccctggg atggccggag aggaaaactg aagcatgacg gggtcaggat tttttccaga
     2581 atttccacca tggcgccggg gattcagtta attttgcctg gtgattttca agaaggagca
     2641 tggcctgttt caggctgtca atgtgcgaat ccttggacaa aagtaaggtg ttcaggtgtt
     2701 caatctcctg tttcagatga cggatcacct gttcactctg gtcagcagag gctgtatcac
     2761 gctgtccaga gacctgtcca gaatggtgtt cacccttcgg ttgctcaaag gggccaaaga
     2821 cacgtaaaag ctctgaagta tctaccattt ttgcattgcg ctcaccgcta caggtggaca
     2881 ggtcacctgc tttaattaac ctgtgcagtg ttgttctact cttacccgtc agtcgggcag
     2941 cctcactaat agacactctg gccatcagtt accatcctgt aatgcatcca gtctcgagga
     3001 cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt cggcctcgac
     3061 atcgagatca agctcgttaa gcttctccag caggagcaga gactgattct ggatgaattt
     3121 agccatgttc agcgctgttt tctgttgttt cgtcataaga ggcctcagag cgtcagtggt
     3181 cagtgtaggg atctcctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
     3241 tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc gagaaaaccg agtaatcgca
     3301 tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc ataggctccg cccccctgac
     3361 gagcatcacg gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     3421 taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     3481 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     3541 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg
     3601 ctgcgcctaa tccggtaact atcgtcttga gtccaacccg gaaagacacg acaaagcgcc
     3661 actggcagca gccactggta actgatttag ggagttagtc ttgaagttat acgccagtta
     3721 aagctaaact aaacgaacag ttttggtgac tgcgctccct ttaagccagt tacctcggtt
     3781 catagagcgc caactctact gaacctttcg aaaaaaccgc ccgccaaggc ggtttttttc
     3841 gttcacagcc ggaagcttac ggctgctgcc agataatctc aagaagaaag taaaaaaaat
     3901 cgattaaaac acaagcaatc aaagttaatc tttgacataa agtctagcta atcacaatga
     3961 aagctgtaca ttttattaag gtactaacaa atcgtatggc atataacaaa aacacgaaca
     4021 gaggctatca tgataaagga aataagttta aagaacttca aatgcttcaa tgagctatat
     4081 ctaaagcaat taaagacttt aaatatcata gctgggaaaa acaactacgg gaaaacatct
     4141 atactggatg ctattttttg tttttat
//
