LOCUS       CP052273                5896 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0258 plasmid pE16KP0258-1,
            complete sequence.
ACCESSION   CP052273
VERSION     CP052273.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609936
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:30:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,394
            Genes (coding)                    :: 5,296
            CDSs (with protein)               :: 5,296
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 47 of 98
            Pseudo Genes (incomplete)         :: 44 of 98
            Pseudo Genes (internal stop)      :: 22 of 98
            Pseudo Genes (multiple problems)  :: 13 of 98
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0258"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16KP0258-1"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5376..5896,1..901)
                     /locus_tag="HJX48_27520"
     CDS             join(5376..5896,1..901)
                     /locus_tag="HJX48_27520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO54116.1"
                     /translation="MKYFSTGFFLKGNVEVKDIINECLSWVSTSPFTSFVPASLDMKRG
                     LDEFDIEVGDERIELINFSDDSIMMSCFRYSKISGAHKWVTDVAVNYYFSEGKTWVQVE
                     SSVVTQQAVHKAPVTKTPLIVLRLLDKFSGGRDDVFKVGIEPVLLDGSEHSLGFACDII
                     NGRHKNRLPVVYVSSKYFFKEHPHNVIPGRLARKLSGLAHVVVEPEGHLFSNKLKHEVN
                     NKNSYGGSVGIYWPGGQSISIHKRGSLSAAEFEKEIFKEIITAMTSLAPLRKNGWSEIV
                     SSKNRKAIQVLKDNGSQSSELASLYEEENESLQDEVKELNQKIASYEARIRVLMERTSM
                     QGGVFLKIGNEYELFEGEVLEIIMSSIRKVLPTNYEGGRYHDVLSSISSANPCSTNLLE
                     RDKKLKQALVGYTKMTPKVRGVLNDVGFSISEEGKHYKITYHGDPRYTYILPKTGSDYR
                     GALNAYSDISKKVFS"
                     /name="hypothetical protein"
     gene            complement(1153..1519)
                     /locus_tag="HJX48_27525"
                     /pseudo=""
     CDS             complement(1153..1519)
                     /locus_tag="HJX48_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3107..3298
                     /locus_tag="HJX48_27530"
     CDS             3107..3298
                     /locus_tag="HJX48_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO54112.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3298..3720
                     /locus_tag="HJX48_27535"
     CDS             3298..3720
                     /locus_tag="HJX48_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO54113.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGPLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            4795..5019
                     /locus_tag="HJX48_27540"
     CDS             4795..5019
                     /locus_tag="HJX48_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="QJO54114.1"
                     /translation="MLAIRLSDEIESRLNLLAKQTGRTKTFYAREAILTHLDDLEDYYL
                     SAETVARVRRGDEVVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            5003..5269
                     /locus_tag="HJX48_27545"
     CDS             5003..5269
                     /locus_tag="HJX48_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJO54115.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRHSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1588..1764
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.264"
                     /coverage="82.0"
                     /evalue="3.5099999999999995e-46"
     Typing          complement(1129..1638)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ttatgtgagc tctaaatatt ttttcaaaga acatccgcat aatgtgatac ctggcaggtt
       61 ggctaggaag ctatcagggt tggcccatgt ggttgtagaa cctgaaggcc acttgttttc
      121 taataaactc aagcatgaag ttaataataa aaattcttat ggtggaagcg ttggtattta
      181 ctggccggga gggcaaagta ttagtattca taaaagaggt agtcttagtg ctgctgaatt
      241 tgagaaagag atatttaaag agattataac tgctatgact tcactggccc ccttaaggaa
      301 aaatgggtgg tctgagatag tcagtagtaa aaacagaaaa gccattcaag ttttaaaaga
      361 taatggaagt caaagtagtg aactcgcttc tctatatgaa gaagagaatg aatccctgca
      421 agatgaagta aaggagctaa atcaaaagat agcttcttat gaagcaagga taagagtatt
      481 gatggaaaga acatctatgc agggtggggt ttttttgaag attggcaatg agtatgagtt
      541 gtttgaaggt gaggtattag aaataataat gtcttcaatt aggaaggttc ttccgacaaa
      601 ttatgaggga ggtagatatc atgatgttct atcttcaata tcttccgcta acccatgcag
      661 tacaaacctc ttggaaagag ataaaaagtt gaagcaagcg ctagtaggct atacaaaaat
      721 gacgccaaaa gtgagaggtg ttttgaatga tgtcggtttt tcaataagcg aagagggtaa
      781 gcactataaa ataacttacc atggggatcc ccggtacaca tatattttac ctaaaacggg
      841 gagtgactat cgaggggcgt taaatgctta ctccgacata tcaaaaaagg tgttttcata
      901 aaccagtaag acatggtgct tgagttcatc tcttgctgga tgtatcttag ttttgaaacg
      961 aaaaaaccac cctggcaggt ggtttttcga aggttagtta atcctggcag attctctaac
     1021 cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttccagtg tagcctcagt
     1081 aaggccacca cttcaagaac tctcgataca tctctcgcac attctgttta ccagtggccg
     1141 ttgccagtgg cgttaagtcg tgtcttttcg ggttggactc aagatgatag ttaccggata
     1201 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     1261 ccttcccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     1321 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc agggggaaac
     1381 gcctgggatc tttatagtcc tgtcgggttt cgccgccact gatttgagcg tcagatttcg
     1441 tgatgcttgt caggggggcg gagcctatgg aaaaacggca gcgccgtatc ctttttttgc
     1501 ccagcttgtc atgccgtatg cctttgctct tatccgtgtg caggtaacgc ccgccgcagc
     1561 cgaacggcag gagcggcagc gaccgagtga gcgaggaggc gtcatatcac cgtaactccc
     1621 attttcttcc ttacgccctg tagctgcatt ttcccctgtc acatgaagca cttttattaa
     1681 gtgttactcc gccgtaacat attgagccag tatacactcc gctagcgctt cgtgactggt
     1741 tcagggctgc gccccgaaac ccgcgaagga ccgtcttttt gtttttttac tgctaaactg
     1801 tcaatatttt ctttcggctg ccgttaactg aggtttcctt gtttgatttt tcctctggta
     1861 tcctgatgaa tatgagtgat gctgaacggc agctggctgt gtcagtgatt tctgactgct
     1921 tacgccagtc tgggggagac tccggcaaaa tgatgctgga tgcttactat ctggtcttat
     1981 ctgagacaga tccccgggtc agggagtgca tgttggcgtt ccttgaatct gttcgtatta
     2041 aatatgtcac cgtctgagtt ttttttctga tttttgaacc atactgtgtg tcaggcggcc
     2101 gtcatggatg atggtcgttg tgacatgtcc tatgtcacgg aagattgttc caatctaact
     2161 tgacttacct tccgggctag ccgcccggtt tttttccgtt cttctgactg tactccagag
     2221 caggttttgc cttttttcta ctgcgctttc ggagtagggg tcagtttgga gatcggaaat
     2281 ttttgtacgt taagcgcatt atttaaccaa tcttggttcg taaggggttg attttgcgtc
     2341 agtggctatc gcttttaagt tgtcgctctt gcagtatcgc atttaggtta tccgattaag
     2401 gtcaaacctc tgaaaatgcc gtatagcgcg ggagtacacc ctattgctat aggtaagtct
     2461 gttcaaaaaa caggcttacc gtacaataat tctctatatc caaactgacc cctgatacgc
     2521 agcttcccgg cgaatatgac ctttttgatt taatggcgca ggttactgaa gagaacctgc
     2581 atgcccctgt ggattccggg ccgcctgtcg gccgggaagc gttatagctt ttcgcttgcc
     2641 cgcgtacgtg gtaatacgcc ggtattacat tttatttctt tattttcatg cgattacagg
     2701 tgattgtaat acgttgcgta ttacataaat aaacggagat ttcagttttt accttttaat
     2761 tcatgcagtt gcgatgaaaa tcgcagctgc gttaggtgta tgaccattta agggccaaaa
     2821 aattatcatt ctggccgggt gatctgcgga ggttaagggg cgtcggtgac aaaaaaacgt
     2881 cattaacgac ctcgttcatg ctcgcatgaa cggattattt ttccggacta caggtaaact
     2941 acagaaagac tacaaaagct gatgacttaa tcctgatttt tagtgcgatg actacagata
     3001 aactacaaaa taactacagg attacctcag atccgttctc tgaaccattc gtacatccga
     3061 cccccttacc ggttttacac tgagcactga tgctctgagg tcccttatga cgaaacaaca
     3121 gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag tctctgctcc tgctggagaa
     3181 gcttaacgag cttgatctcg atgctgaggc cgacctttgt gagaaactcc acgatgatgc
     3241 ggagcatctt ttccgtacac tgtcctcgag actggatgca ttacaggatg gtaactgatg
     3301 gccagagtgt ctattagtga agcagcacgg cttgttaagg ttagtcgccc tactatttac
     3361 aagatgatta acagtggtaa gttaagttac acctctgttg taaagcacgg gaagggtatt
     3421 aaagttattg atacatcaga gcttattaga gtatttggtc ctcttgatgg tgtaatcgat
     3481 gctgtaaaat atgatgtaaa aagtgacgca gaatctacag gtattaacag cgttggttta
     3541 catgatttac aacatagaat cgctttactt gaagctgaaa acgatggtct taaaggggct
     3601 gttaaggctc gagatgagca tattgattcc cttcgtcagg ctatgcagct tctggagcac
     3661 aagcacgaac cttcttcacc accccattcc ccgtggtgga aattctggaa aaaatcctga
     3721 tcccgccatg cttgagtttt cctctccggc catgccgggg cgttcgtgaa ccttcccaaa
     3781 aatcgccaga tctctgaacg ctcctcacac taaaaatatc cacaccccac gcaaaattaa
     3841 atttttgcag ataactcatt aatatcagtc agttggttgt tattaaatta cgcagtatta
     3901 ccttctgtaa ggtcaatcca agtcgtcggt gattcgtgta cgacttgccg acgattcgtc
     3961 atagagtcat cgacgactca ctccacacta tcagtaactt atgacgactt gactctagag
     4021 tcgaatcgtc gctttggcgt ttttttgacc gcggtgactc tagagtcaag tcacgagtcg
     4081 aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc
     4141 gtcgacgaac ccgattcctg atcgtgctga tacggggttt ccggctgtgc cggcaacgtc
     4201 tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cgggtaagaa gaacatcgga
     4261 aacaggactt accccggctg aatggcgtga atttccgcgc tatgcacttg cgcgcatact
     4321 catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc
     4381 ctgttttacc ggtttcccgt cagaaacgcc ctgagagcct ctcaggcggt gcacgcaacg
     4441 gatgttatgt ttaatccgtc tgaccgacaa gtacaaaaaa agcccctaag ggctttgcta
     4501 acaactactt tcctgaagat ttacttggtt ttaacttgtc agtatttccg ggagtttttt
     4561 tattgtcgcg ttggcgctgg tcaggctgcc ctgttgattc tgcaattcgt tttggtccag
     4621 gtttaagagc cataatggat atccttcatc gtgttaagag gaaactctat aactgcatat
     4681 atcgacagtt gtaggtagtc aagatgaatg gaaagattgt tttttgttct ttgattttta
     4741 atgttgtata attttgttat acatgtatat caatttggat tcagagaggt ctttatgttg
     4801 gctatcagat tgtctgacga gattgagtcc cgtctaaact tgcttgcgaa gcaaacaggt
     4861 aggactaaaa cgttctatgc gcgtgaagcg atcctgacgc atctggatga tcttgaagat
     4921 tattatcttt cagcagaaac tgtggcacgt gttcgccgtg gggatgaagt tgtacattcg
     4981 tctgaagacg tgaggaagtc gcttggtctg gacgattaac tattccgacc gagcgctcaa
     5041 gtcgttacgt aagatggaca agcagaacgc acgacggatt gtggatttta tggatttacg
     5101 tattgccgtt gccgcagatc cacgccactc aggtaagccg cttaaaggtg agcttggaga
     5161 attctggcgc tatcgagttg gcgattatcg catcctatgc gagatcaggg acgacgagct
     5221 gattattctg gctgctacca ttggccaccg acgtgaagtc tacgactgaa gcccgctttg
     5281 cgggtttttt ttatgtgagg cgtgaaaaat atgtcgttaa caaaatggct ttttaatttt
     5341 aaaaaatatt ttactctaaa tcataagggg tgtttatgaa atatttttcc acgggcttct
     5401 ttttgaaagg aaatgttgag gtaaaagata taatcaatga gtgtttgtca tgggtgtcaa
     5461 catcaccatt tacttcattt gttcccgcta gcttagatat gaaaagaggg ttggatgagt
     5521 ttgatattga ggtgggtgat gaaagaattg aactgataaa tttttcagat gattcgatta
     5581 tgatgtcttg ttttcgttac tctaaaatat caggggcaca taaatgggtc accgatgttg
     5641 ctgttaatta ttatttttca gaagggaaaa cctgggttca agttgaatct agtgttgtta
     5701 ctcagcaagc tgttcataag gcacctgtaa ctaaaacacc actcattgta ttgcgtctgc
     5761 tagataaatt ctctggtggg cgtgatgatg tttttaaagt aggaattgaa cctgttttac
     5821 tggatggttc agaacattct cttggctttg cttgtgatat aattaatgga aggcacaaaa
     5881 acaggcttcc tgtggt
//
