LOCUS       CP052271                3559 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0268 plasmid pE16K0268-3, complete
            sequence.
ACCESSION   CP052271
VERSION     CP052271.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609937
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:46:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,647
            CDSs (total)                      :: 5,523
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 53 of 126
            Pseudo Genes (incomplete)         :: 65 of 126
            Pseudo Genes (internal stop)      :: 22 of 126
            Pseudo Genes (multiple problems)  :: 11 of 126
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0268"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16K0268-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(3508..3559,1..140)
                     /locus_tag="HJX49_28205"
     CDS             join(3508..3559,1..140)
                     /locus_tag="HJX49_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO81180.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            140..562
                     /locus_tag="HJX49_28210"
     CDS             140..562
                     /locus_tag="HJX49_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO81181.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1426..1650
                     /locus_tag="HJX49_28215"
     CDS             1426..1650
                     /locus_tag="HJX49_28215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO81179.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            1652..2296
                     /locus_tag="HJX49_28220"
     CDS             1652..2296
                     /locus_tag="HJX49_28220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO81182.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            2296..2772
                     /locus_tag="HJX49_28225"
     CDS             2296..2772
                     /locus_tag="HJX49_28225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO81183.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2979..3092)
                     /locus_tag="HJX49_28230"
     CDS             complement(2979..3092)
                     /locus_tag="HJX49_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJO81184.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(3117..3551)
                     /locus_tag="HJX49_28235"
                     /pseudo=""
     CDS             complement(3117..3551)
                     /locus_tag="HJX49_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     Typing          complement(2980..3387)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 agtctctgct cctgctggag aagcttaacg agcttgatct tgatgtagag gccgaccttt
       61 gtgagaaact ccacgatgat gcggagcatc ttttccgtac actgtcttcg ggactggatg
      121 aattacagga tggaaactga tggccagagt gtctattagt gaagcggctc ggcttgttaa
      181 ggttagtcgc cccacaattt acaagatgat taacagtggg aaactaagtt acacctctgt
      241 tgtaaagcat gggaaaaata ctaaagttgt tgatacatca gagcttatta gggtttttgg
      301 ctcgctttat ggtgggggcg acactgtaaa acatgatgta aaaggtgacg caggcgctac
      361 aggggttaac atctctggtt tacatgattt acagcataaa attgctttgc ttgaagctga
      421 aaacgatggt cttaaagggg ctgttaaagc tcgtgatgaa catatcgatt ctcttcgcca
      481 ggctatgcag cttctggaac acaagctcga accctctcca caggttcatt ctccgtggtg
      541 gaaattctgg aagaaatcct gaccccgtca tgcttcagtt ttcctctccg gccattccgg
      601 ggagttcctg aaccttccca aaaatcgcca gatctctgaa cgctcctcac cactaaaaat
      661 atccacaccc cacgcaaaat taaaattttg cagatttgta ttttatatca gtagtttacg
      721 ttctataaat ttcgtttttt taattttatt tatacagatc cgactctatg acgactcgtc
      781 atagagtccc cggcgagtcg tcatagagtc ggtaacgact caggactata aaaacaataa
      841 gttaacaacg acccgactct agacccgact ccccgtttgg ttgtttttta gacgtgacga
      901 ctctgggtac gactctagtg aagagtcgcc ggtgaatcgt gtggtgagtc gtccggcgac
      961 ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaacgtgc tgaaaaccgg
     1021 ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg
     1081 ttttcggatg ggaagaacgc cggaaacagg acttaccctg gctgaatggc gtgaattttc
     1141 gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg
     1201 gcgggttttc gggtggtttg ttgcctattt taccggtttc ccgtcagaaa cgccctgagg
     1261 ccgttttagc ggtgcgtaca attttggtgt tatgttaaat catggccaca aaattccata
     1321 aagtgaccaa tgtaatgtta aatgagcatt gtggatgaaa gatgttgttt tatggtatta
     1381 ttttttatgt tctaaactta aatttatttc tatcggggta ttataatgag caataaagat
     1441 tctttagaaa gaaaagtaac accaagaaaa aatgaacctg tattcccgat ggctctaaga
     1501 gttgggatag ctgaagatat ttgtttttta gagttagtgg actttcctga aaaagaatca
     1561 ggaagggttt tttattccat agctctaacc aaaaaacatg caaagaacct tatcgatgga
     1621 ttgaataaat tcgttgagtc aggtgattag tatgaaatac attaaccact gcgatagtga
     1681 agattattat attactaatg atagcattga tctcggtgtt cgttatgatg tttctgatta
     1741 taataagtta gagctaaatg atttaagaga tccggatttg tttaaagttt tgggtcgagc
     1801 atttgttgtt gttgagaatt ctgtttggga gtcttggaat gatgatgtta aaacattaga
     1861 ttgctctggt gacggttatg agtcaatttt aaaacattac gaatcattag aattatcagg
     1921 tgaattctta ttgtttttta aaaaaataag aagtgccatt tctgtttata atagagaata
     1981 cgagttctgc actggtgacc tagaaccatc tttgcaggct attgttcagt tgattaggta
     2041 tgcccctgaa cttgctgcaa aaaaagacgc tcagatttac tttgataaaa aatcagagtg
     2101 ttttgggctt atatataaac aaactaagaa aagcaaaggc acgctaaatt taatagtgaa
     2161 aacaaataac gagattattt tttcctttgt tagaaaagtt tctggagtgg ttaaggttac
     2221 aggtagggca tatattggcg atagccttga taactccatt gctatcaaat cattatttaa
     2281 aatgatggag tggtgatgac tgtgttatca actttaaata acctcaaatc aatcccttgt
     2341 attaatgcat ttacgggcag agcaaataat tcatatttat ggcgttatgt tatggaagat
     2401 cataaagatg agtttggcgc tacaactatt aagtgttttt atcttaggga ggaaatttca
     2461 gaggattatg tgtctattta tcattcaaat aaagattatt atgaaggaat ggtaagggat
     2521 gtacataaaa gaatccctgt agggaaaacc aaacctgcca tatatctctc atttgatgca
     2581 gcggaagtga tcgagcaagt taacaaacca gataaatgta ttgatataaa ggacgaaggt
     2641 taccctcact tgggtttgtg gtatttattt gatattaatg atgaatttac tttgattgag
     2701 gtgactacaa ctattttgga attatcctct ttttttcaaa gaaatgaagg tggcgtggtt
     2761 gttcctcttt agcgaaaacc gtcttgacgt catcaagtgt gacctagtac tcttcttgaa
     2821 aaagaaaaac cgcctttgcg gggcggtttt tcttaggttc taagagtttg cggctctatg
     2881 aaccaaggta actggctagt tagaggagcg cagtaaccaa aatcgttctt tcagtttagc
     2941 cttaactggc acataacttc aagactaact cctctaaatc agttaccagt ggctgctgcc
     3001 agtggcgctt cgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggaaaaggcg
     3061 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     3121 ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc
     3181 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg
     3241 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg
     3301 cttgtcaggg gggcggagcc tatggaaaaa cggccgctgt gcggcctctc cttctcccca
     3361 tcacgcgatc gctcggtttt ctcgagcttt tatacgcatg ctcctgccgc tcgccgcagc
     3421 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaagagaa catccctaca
     3481 ctgagcactg acgctctgag gcctcttatg acgaaacaac agaaaacagc gctgaacatg
     3541 gctaaattca tccagaatc
//
