LOCUS       CP052267                4642 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E16KP0287 plasmid pE16K0287-2, complete
            sequence.
ACCESSION   CP052267
VERSION     CP052267.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609938
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:36:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,552
            CDSs (total)                      :: 5,426
            Genes (coding)                    :: 5,307
            CDSs (with protein)               :: 5,307
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 63 of 119
            Pseudo Genes (incomplete)         :: 61 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 29 of 119
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E16KP0287"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE16K0287-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4493..4642,1..60))
                     /locus_tag="HJX50_27730"
     CDS             complement(join(4493..4642,1..60))
                     /locus_tag="HJX50_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="QJJ78332.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="DUF1471 domain-containing protein"
     gene            448..630
                     /locus_tag="HJX50_27735"
     CDS             448..630
                     /locus_tag="HJX50_27735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ78328.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            793..2136
                     /locus_tag="HJX50_27740"
                     /pseudo=""
     CDS             793..2136
                     /locus_tag="HJX50_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849282.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /name="cytidine deaminase"
     gene            complement(2529..2642)
                     /locus_tag="HJX50_27745"
     CDS             complement(2529..2642)
                     /locus_tag="HJX50_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QJJ78329.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2667..3101)
                     /locus_tag="HJX50_27750"
                     /pseudo=""
     CDS             complement(2667..3101)
                     /locus_tag="HJX50_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            3058..3249
                     /locus_tag="HJX50_27755"
     CDS             3058..3249
                     /locus_tag="HJX50_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ78330.1"
                     /translation="MTKQQKTALNMAKFIQNQSQLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3249..3650
                     /locus_tag="HJX50_27760"
     CDS             3249..3650
                     /locus_tag="HJX50_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJJ78331.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASAHQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2530..2937)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcaccgaaa gacatcatgg atagtgcggc aactgcaacg aaagttttga tggatttcat
       61 ggtcgtattc cttaagtaat tttgttctgt atggggcgtt gttgccctga tgtgataaat
      121 gataggacga ccatggactg attaaaatcg aaacaatttg cggatatcat tcaaaaaaaa
      181 agaacaagaa ttgagcgcat caagaccaga ggtttatctc tggattctag gtaccaaatg
      241 aggtggtgga tagaaatgtt aacattgatg attttatttt aacaaattga aaaataatgg
      301 ttagtttggc gtgatctctc ttgcttttct cttgccgaat ttcctaatgt ttaggataag
      361 aaaaaaaaat ctctatcgat tacgtttctt ggctattgcc tgttataaag ataacagtaa
      421 aatccatgcg tcttgcagga gggtgtgatg ggtaaagagc tatctctaaa taaaatttat
      481 gataagaatt ctagatatat taatgattct agagggcatg agctgggaaa ggaaaagaaa
      541 gttttcgatt ttgaagttga aatgattaaa aaaagaagag aggacgctat aaggaagata
      601 attgaggatt caaaatcatt aaaatggtaa gtgaggtctt ttatgagcct ggctagaaag
      661 ttaagagaaa aaagaagtaa tccgattcat agtgctaacg aggactcctc tggaggggat
      721 agcataaacc aaataacctc cagacagtct atggatcttg ttataggatt ttgcggtgct
      781 gttggctcag gaataaagtc aattgttgat gtgactgaag aagaactaga tgatctagga
      841 tatgatgttt atcgcataag agttagcgag cttatgcaaa aacttttttc agaatggcgt
      901 gatgatattg ttactccgtt tgatagatat cataagctgc aagattttgg tgatgcgtta
      961 agaaataaat attacccaca tatactatct gaagctgtaa tccatgaaat aaaaatagaa
     1021 aaaggcattg acacctcaag taaaaaaaat aaaaaggctt ttttggtcga tcagttgaag
     1081 catcaggatg aaattgaact tttaagaatg gtctaccaac ataattttta tttactaggt
     1141 gtaattagaa gtgaagtaga aagaaagcgt aatcttagag atgaagggct gagtaagtca
     1201 gaagtcgata tagttattca tcacgataga aaatcagaga gcgattatgg tcagcagact
     1261 gcgaaagcta tacttgactc tgattatttt gttaagaata atcaaggaca aagggcgcac
     1321 cttaaggcta aggttaagcg ttttattggc ttgatacatg ggctaaatgg tttaactcca
     1381 accacgtatg aaaaaggtat gtatgcagct cattcagcat ctctgcaatc tgcatgcctt
     1441 tctcgtcaag ttggtgctgc tatattggat gttaatggga atctgatttc cgttgggcgt
     1501 aatgatgtac ctaaattcaa tggtgggctt tatagtgcgg atgatggtgt caatgatcat
     1561 cgttgtgtat acaaaggtgg taaatgttat aatgaagtta gaaaactaaa gattaaaagc
     1621 aaaatttcac aaatacttat gtcggaaagc gcttgtgaac ttggtgttaa attaacagaa
     1681 gatcaagcag agcaactggc tgatgtaata tataaaaaaa caccagtatc ttctattata
     1741 gagtactctc gctcaattca tgcagagatg gatgctatta catcattagc tagactaggt
     1801 aatggaggtt tttctgataa agttttatat acaaccacat ttccttgtca taactgcgcg
     1861 agacatattg ttgctgttgg aataacaaaa gttgtttata tagagccata tgaaaaaagc
     1921 ctagctttag aattgcatga tgatgctata accgaggtga atgagcatgg gaaggttata
     1981 tttgagtcct ttgaaggtgt atcacctcgt aggtatcaaa aattcttctt ttctacagat
     2041 gaaaggaaag atagtttcgg taacgcagaa aagtactcca ctaaatataa aaatcacatt
     2101 gatatacagt ttattgatag ctatttagat ttcgaaaatc gagtcgttga tatttttaga
     2161 actaacacaa atattgattc tggcaatcat gtcgggagaa ctgattcgac tattaattta
     2221 tcttcccagc ccgtaccgcc cgtaccgccc gtaccgcccg taccgcccgt gccgcccgtg
     2281 ccgccagtac caccagtacc accagtacca ccagactcga aatgaagatc tgttgactct
     2341 gatgttatac agagcttaac cttgagacga gaaagaaaaa aaccgccttt gcggggcggt
     2401 ttttttaagg ttctagagtt tgcggctcta tgaaccaagg taactggctt ggaggagcgc
     2461 agtcaccaaa acttgttctt tcagtttagc cttaactggc gcataacttc aagactaact
     2521 cctctaaatc agttaccggt ggctgctgcc agtggtgctt ttgcatgtct taccgggttg
     2581 gactcaagat gatagttacc ggataagacg cagcagtcgg actgaacggg gggttcgtgc
     2641 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2701 caaacgcggc cataacagcg gaatgacacc ggtaaaccga atggcaggaa caggagagcg
     2761 cacgagggag ccatcagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgccg
     2821 ccactgattt gagcgtcaga ttccgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2881 cggccgctgt gcggcctttc cttttcccca tcacgagacc gctcggtttt ctcgagcttt
     2941 tacacgcatg ctcctgccgc tcgccgcagc cgaacggcag gagcgtagcg actgagtgag
     3001 cgaggaagcg gaaaagagac tgttcttaca ctgagcactg acgctctgag gcttcttatg
     3061 acgaaacaac agaaaacagc gctgaacatg gctaaattca tccagaatca gtctcagctc
     3121 ctgctggaga agcttaacga gcttgatctc gatgttgagg ccgacctttg tgagaaactc
     3181 cacgatgatg cggagcatct tttccgtaca ctgtcctcga gactggatga attacaggat
     3241 ggtaactgat ggccagagtg tctattagtg aggctgcccg actgacgggt aagagtagaa
     3301 caacactgca caggttgatt aaagcaggtg acctgtccac ctgtagcggt gaacgcaatg
     3361 caaaaatggt agatacttca gagcttttac gtgtctttgg tccctttgag caaccgaagg
     3421 gtgaacacca ttctggacag gtctctgaac aacgtgatac agcctctgct caccagagtg
     3481 aacaggtgat tcgtcatctg aaacaggaga ttgaacacct gaacacctta cttttgtcca
     3541 aggattcgca cattgacagc ctgaaacagg ccatgctcct tcttgaaaat caccaggcaa
     3601 aattaactga atccccggcg ccatggtgga aattctggaa aaaatcctga ccccgtcatg
     3661 cttcagtttt cctctccggc catcccgggg agttcctgaa ccttcccaaa aatcgccaga
     3721 tctctgaacg ctcctcacga ctaaaaatac ccacacacta cgcaaaatta aaattttgca
     3781 gatttgtatt taatatcagt agcttacttt cctgaaattt cggtttttaa aatttattta
     3841 catagatccg actctatgac gactcgtcat agagtccccg gcgagtcgtc atagagtcgg
     3901 taacgactca ggactataaa aacaataagt taataacgac ccgactctag acacgactcc
     3961 ccgtttggtt gttttttaga cgcggcgact ctgggtacga ctctagtgaa gagtcgccgg
     4021 tgaatcgtgt ggtgagtcgt ccggcgactt gccataacgt gattgccggc cgtcgacgaa
     4081 cccgattcct gaacgtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc
     4141 acgcaataaa ttgcgtcccc gttcaccgtt ttcggatgga aagaacgccg gaaacaggac
     4201 ttactctggc tgaatggcgt gaattttcgc gctatgcact tgcgtgcata ctcatgcatg
     4261 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta
     4321 ccggtttccc gtcagaaacg ccctgagacc gttttagcgg tgcgtacaat cataccttta
     4381 tgttaaatga cagatgcaaa aaagcccccg aaggggcctg aaggttttcc tgctaaggga
     4441 cgaaacgggc cgcctcccgg cgacctgttc ggcagctccc tctgcgacca tcttatttgg
     4501 tcagttcagc cgtcatgtgg acacggttat tgaactgagc gctggtgatt ttgtatgacg
     4561 caccttgttc agcggcctgt gcggcaattt ttgcttcagc acgatccagg gtggaggcgg
     4621 tagcgctgac gctctgagca aa
//
