LOCUS       CP052238                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0029 plasmid pE17KP0029-3,
            complete sequence.
ACCESSION   CP052238
VERSION     CP052238.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609945
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,832
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 68 of 144
            Pseudo Genes (incomplete)         :: 77 of 144
            Pseudo Genes (internal stop)      :: 19 of 144
            Pseudo Genes (multiple problems)  :: 18 of 144
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0029"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE17KP0029-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(8939..9294,1..22))
                     /locus_tag="HJX57_29110"
     CDS             complement(join(8939..9294,1..22))
                     /locus_tag="HJX57_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ30802.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            91..270
                     /locus_tag="HJX57_29115"
     CDS             91..270
                     /locus_tag="HJX57_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ30792.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(298..828)
                     /locus_tag="HJX57_29120"
     CDS             complement(298..828)
                     /locus_tag="HJX57_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ30793.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(835..1566)
                     /locus_tag="HJX57_29125"
     CDS             complement(835..1566)
                     /locus_tag="HJX57_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ30794.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(1566..3530)
                     /locus_tag="HJX57_29130"
     CDS             complement(1566..3530)
                     /locus_tag="HJX57_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ30795.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5011..5160)
                     /locus_tag="HJX57_29135"
     CDS             complement(5011..5160)
                     /locus_tag="HJX57_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ30796.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5245..5502)
                     /locus_tag="HJX57_29140"
     CDS             complement(5245..5502)
                     /locus_tag="HJX57_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ30797.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(5512..7197)
                     /locus_tag="HJX57_29145"
     CDS             complement(5512..7197)
                     /locus_tag="HJX57_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ30798.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            7600..8019
                     /gene="exc2"
                     /locus_tag="HJX57_29150"
     CDS             7600..8019
                     /gene="exc2"
                     /locus_tag="HJX57_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ30799.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            8325..8588
                     /locus_tag="HJX57_29155"
     CDS             8325..8588
                     /locus_tag="HJX57_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ30800.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            8578..8877
                     /locus_tag="HJX57_29160"
     CDS             8578..8877
                     /locus_tag="HJX57_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ30801.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             512..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4330..4908
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(839..1566)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gtctcaccgt cacccacgcc attgtctcac cccgctattt ttgagacagt atacctgaca
       61 ggcgcacacc atgcccggaa ggaaagaatc atgacaaaac aggaaaaaac agccctcaat
      121 atggctaaat ttattaagga gcagtcgctg ctattgctcg ataaactgaa tgagctggat
      181 atggataccc cggccgacat gtgcgagcgg ctacacgagg atgcggaaac actctggcag
      241 gtgatgcggg ataagctcgg ggaggaataa cagcaccggc atggccggtg ctggcattta
      301 ttttttgccg aacggccacc accggcgggc aggtttgtcc tccggcagtt caggtaccgg
      361 cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac
      421 cgcgctttcc ccgtgccgct cactcaccac gtcaatcatc gcgttaatct gctccggtga
      481 gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccaccttgcg
      541 atagaggtaa tcacacagct tcattcctgt gggtttgttc cgttcccatt gcggattttt
      601 accagtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat
      661 ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt
      721 ctgccaggcg agcaccacca tatcttccag atgctcgctg gtcggcatga tgcagcgctc
      781 gccgttccgg tcctccgcgt cttcaataaa taccatgtac agcgccataa tccctcactc
      841 cgttgtcata aatttaaagc acgcaaacaa ccgttcacga tccagctctg ccgttttgcg
      901 ggcctcgccg gtctgtgggt tcacgatttc atccaccgac aggattgccc gctgcaccct
      961 gacctgcgtc gcccggtccg ggcagatgta ccagatttca tcgatgccgt tagctttgcg
     1021 gttgaacaga tacccggaca ccacttcctg ataccggcga cggctcttga cggtgcgctc
     1081 cacctcaaac gccaccgttt taccgtccag ctccgccagg gcgtccggca ctttcatgcc
     1141 tttaagactc agacggtgca gaatgcgcca gccggtggcc cctctggctt ccatatcaag
     1201 gcgcagggat tgcacggcca gccggtgcgg cagatgtgct gccgacaccc ttgacggctg
     1261 aaacggaata acatccgcca gcggttcgtc ctcgtcgaac gagaacgcca cgccatgcgg
     1321 cgtcagcccc cacacgctga cctgacggcc gtccaccgga tatttggtcc ggaccagcag
     1381 ccctttcgcc accaggctgt cgaggaagcg gtagaccggc tgcaccgccg caatccccag
     1441 ccgccgcgca accagctccg ccgtggaaca cagctcttcc cgcaaccagc gcagggtcag
     1501 ccgggttttc tccgcgttcc gcgcctgtcg tttcgcgtgg ctgacgttgt acagctccag
     1561 cgccatcagt aaatccccgc aaaatcatca tccgctttcg gcccgccgcc gtcggccggg
     1621 ggccggaact ccgccagggg atcggttttc gccagcaccg ggaaacgctg caacgggatt
     1681 tcccggcgct caacccgcag cggactgctg aacgccatcc gggccacacc cagcccggtc
     1741 atgaccgcga acccgtccgg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc
     1801 tccttctgga tgaagctgtc cccgctgata tgctccacgt tccccgcctc ccggctggca
     1861 cggcggcgct ccacgtccac cctgatttcc ccggtctgcc ccgccgccca ctgcgcggac
     1921 tccaggctgg ccgcccggta aaaccagcgg atcggcgtgt tatccagtac cgtggttttc
     1981 acaaattcag cgggcagatc ctgcccgcac tgcccgaaat cgcccagcga ctggtgcgcg
     2041 agcaacagat tgcagtcccg gctgcgcagc gtccccagtg cgttcagtac atacttcgac
     2101 agcagatatt taatttcatc gagcatcacg ctgacgtgtg gccactcccg gaactcgtcc
     2161 cgcgcaatga tgatttgcgc gcagcgggca aacagcattt tttgcacacg gatcaccgcc
     2221 tcgtcgtcca tcgagccgat aacgtacagg cagccgccgc cgttgataat ccccgccaca
     2281 tcgatgccac tgtccgtctg caggacgctc aggtcagcga cgttttccag cctagtgatc
     2341 aaccccttca cgtccttttt cagggcctcc ggcagcgcgt acgccgccgc cagcacctgc
     2401 tgaatgtttg ccccctgcgg aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg
     2461 atgcggtagt ggtccgccac gtcgccggtc tcgcccaggt taagcccggc aatcagcagg
     2521 tttttcagcg cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc
     2581 cgcagatcca gcagggtgaa gtccggacag tgcgcccgga agacgtgcgg cgcccatgcg
     2641 tcccctttgg ggtcgaagta caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc
     2701 gcgttggtgg ccatcacgct cttgccgctc ccgggcaggc cgaggatctg aatgttggtc
     2761 ttgcgccagg tatcacgcgg aaccagcacc ggcttgccct gctcatcctt acccagaaac
     2821 accgcctgct cctgccgggc ctgctccgag gaaaaatcgg ccagcgcatt aaccggtacc
     2881 ggctccccga aatgcaggtg gcgcacgtcg gtcatctctc ctgtccgctg gctgctgcgc
     2941 cggctctcct gattcagttt cgcgttcacc gcctcgtcag cagccgcaaa atgaccgtca
     3001 agcagcagga acgccagcag ccgcgccaac acaatcccga cagccagccc gcccgcgcac
     3061 caggcaatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt tgcgtagagg
     3121 tccggacgca ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc
     3181 agaagggcca gaccggccgc gccggtcagc cagtacgcgt agttccagac atgtcgacgt
     3241 gccctgacca tccgcccggg cgaatccagc gaggccagca ccgaaaatgg cgctggccgt
     3301 ttattatcgc gcttatagcg atcggcatac tcccgtcgct ccttatgact cagccccaca
     3361 aaacgcatcc gtccggccca aagttcagcc agaccgaaaa acaggccgat aactaggaaa
     3421 gccccggcca gaccgtaata ctcctggtac agccagctga cccacggata aatctgccgg
     3481 gccagctccg gaatgtgcca cttccaggca agcgaatccg tattaaacat gttctctctc
     3541 cttcagacga tggtttgccg gcgtgatgtg cagcccccgc tgcacaagcc gcggacatgc
     3601 cccaaggcat ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt caccacggag
     3661 gccagcctct ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc
     3721 ccagcgtgtg agaggaaaag gcggcacccc agtgcggtct tttccggtgt tccggccccc
     3781 gtcagagttc gccatagcga gcggctgacc acccgcaaaa tcaggagcag ctttgctgct
     3841 tcgccaggtg cgtccagcac cgtggatttt tgtgggtggt caggcgtgag cctgacgggg
     3901 gccgggtgaa tttctggcgt ggccggcgtc cagccggacg gagccagaaa gagggggaga
     3961 gaccgtgcgt cggtggcgtc cagccagcgt aagcagggga tatccccctg aggtcagcca
     4021 cgccctcgcg gggatgacct gtatttctcc gtaaggagaa aaaagcaaca gagataggtt
     4081 aacgtaaaat gttcagcgaa aaaccgatat gattcaccta tcctggtaat ttattatcag
     4141 ggaatataaa gacttaatca ggggacaata tgccacatca gcgcgtccgg tgtccgtctt
     4201 gtaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga
     4261 ctgcggcggg cgctgcggac acatgcaaag ttacccacag attccgtgaa taagcagggg
     4321 actaacatgt gaggcaaaac ggcaaggcca cgcaggtggc gtttttccat aggctccgcc
     4381 ctcctgactg agttcacata aacagacgct ttttcggtgc atctgtggga gccggtggct
     4441 caaccatgaa tcagtcagga gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc
     4501 cgatgaatcg ctccctcttg cgctctcctg ttccaaccct gccgtttccc ggatacctgt
     4561 tccgcctttc tcccttgcgg gaagtgtggc gctttctcat agctcacact ctggtatctc
     4621 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaaccccc cgttcagccc
     4681 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa gcacgcaaaa
     4741 cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt agctaaacac
     4801 gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag atttggttgc
     4861 tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact taaccttcga
     4921 tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa
     4981 aaggatctca agaagatcct ttgatctttt ctactgaacc gcgatccccg tcagttcaga
     5041 ggaggacgat ggtgccaccg ttccaccctg aacatcccgg atatagtttg cctgacatgc
     5101 gaccagaaga aagcctgaaa aaatcaatat aaaaagaaaa acaaccttta cctttttcat
     5161 ataccttccc tgtatttaaa aaccccggta ttcatccctg aacaccggaa gtctgtattt
     5221 caaatcatcc ttgataactt ttgcttacca gttaccatcc cggtaaacaa aggatacaaa
     5281 gtaatcaaat ttactaatat cgattacatt tttaaagtgt ggctgtaata tcgaaaccca
     5341 tgatttttca acatcaaacc aaccattatt aatattatcc tttaaaggca tccccagact
     5401 ttcaatgaca gaaccatcat caccgaagtc ttttgagtat tcaccacctt taaactcttc
     5461 ggttttctta tcaaaccaat gaatatgtaa tttaagcccc atatttacct cttaaagata
     5521 ttttttaatg tttcgttttg gatccggccc tttaacctgc ttacccgttt ttggatcgaa
     5581 tgccccgagg tgttcgccat cactggcgcg gtacccttca agctcaccgt gctgggaatc
     5641 ccattcataa attttacgct ttttatcacc ataccatcgg gcccgcttac caccaccacc
     5701 ttgtttaggg gttttaggtt taccctcttt caactctccc aaccccttaa tatcttcagt
     5761 cttgggatca ggaaaataat catggccgta atctttagtt cccttacgag gttttttctt
     5821 ttcctcattt aatttatttt ctgcatcctt cgcttttttc tccttttgtt tccggctctc
     5881 catcgccccg gaaagcgcag ccttatccct ggataaggat tcattcattt cgtttaaaga
     5941 tgtctgggca gcttttaatt tctcattctg attctgaata tccttatcaa gatagcgaac
     6001 ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa aatcatcttt
     6061 atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc ttgcaccggg
     6121 gatcgccttc tctgtcgatg caatgcgact gtttaaggtc gcaatatttt tatcttcagc
     6181 atccagctca gctttcgctt tatcatactc tcttttcagc ccctcttccg ggtgagcagc
     6241 gtcccatgcc tgctggcgac gcttttcttc ctccagacgc tgttttacct gtgccggagt
     6301 aagaacatcc gtcaccgaca catagaccgg tttctgtccg ctctctttcg ggaaacgaat
     6361 aacggcctca cgggagttac caccggccgt aaaaccagcc ggacgtgaat cacctttttc
     6421 agcaataatg ccttttggcg gggctttcgc ggccggaaca cctttaggta cgctcacctg
     6481 cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg tcggtttcgc
     6541 atccaccaca ggaacactca ttggccggcc ggtgacaacc gcaatatgct gccgctcgtc
     6601 cttcacaata tccgccacac gtttagtgac gctgaccgtc gcctggtcca gcggcaggga
     6661 gctgaccggc gtctccgtta ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat
     6721 tttcggatca tcttttgcta tctcagaagg cagcactccg taaatcgcaa tcccccacag
     6781 accaaactta aacggacctt tcagggcagc cagcacatca gcaacagcgg ccgacaacgc
     6841 atcgccggaa acggataaag ccagtccttc agcccccgga gtggccaggg caggcagacc
     6901 gaaggccatc gcggtggccg aagactgtcc gccaccagag ctcccgctat gattgccgct
     6961 gttaccgcca ttaccaccgg gtttactccc gccgttacca aactcccccg ggttatagtt
     7021 atgaatatcc ccgttaggtg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt
     7081 accaccacct gatgaagggc cacctttacc tgatgtaccg ttcacattcc cactggcctg
     7141 accaccattg tgtcccagac ctgaattacc cggacctcgt ccgtctccgc cactcatata
     7201 cttttcctct ttgactttta aaacaataaa ctaaaataaa atactgtaca tataaccact
     7261 ggttttatat acagtataaa aattacgctg ccttgttttt catgtcaagt ctgtaaattt
     7321 gcgtttttgt gaaaatgatc aaaaaaacag ccactcacac catgcgcaat cccctccaga
     7381 tgcaacaaaa accagcacaa acggcgccgt tctccggcct cagtatgttt tactgaaatc
     7441 attatatgag ataccagaaa gccccctccg ggacaggaaa agaaacgggg ggtctggttc
     7501 gggttgttcg gtcactgaac acggtcagaa actgatgata agatagcctg cggatatata
     7561 actcaatgag aaaaaagaat taaaggtaag gagacgctca tgagacgttc cggcagcaaa
     7621 ctgtgtctta tcgccatgat aacagcgaca gcaactctca ctgcctgtgc tccaaagggc
     7681 agcgtggaac agcatacccg gcattacgta tatgcttcag atgacggttt tgatcccaat
     7741 ttttccaccc agaaagctga cacaacacga atgatggtgc cttttttccg gcagttctgg
     7801 gatatgggcg caaaggataa agcgaccgga aaatcacgga gtgatgtgca gcaacgcatt
     7861 cagcagtttc acagtcagga atttttaaac tcactccaag gcacaacaca atttgcgggt
     7921 accgattacc gcagcaaaga cctcacaccg aaaaaatcaa ggctactggc tgacaccatt
     7981 tcagctgttt atctcgatgg ctatgaaggc agacagtaag tggatttacc ataacgcgtc
     8041 attacgcgca ccgctcagac gggctcaggg cgtttccggc ggctacggga taaaacaggc
     8101 aacaaccccc ccggaaaact gccggaaacg cgcccgataa attttatcct aatgcatgcc
     8161 tatgcacgca actgcatggc atatgttttg acgtaaaatg gatgccgtaa gccctttttt
     8221 accgactttc tgcttcatca gcctgactga cacgaccata ttctttcaga ccgccccctg
     8281 ctaaacttcc ttcatattgc cgcattaacc cgggaggctc cgcaatgagc aacactacac
     8341 actacgaaaa cgccaacttt cttcgggaac tggctgaaag cctgccccgc atactgccgg
     8401 aaggtggccc ggacaaagct gcgctgttgc agcgactggc taacgaagaa ctggcccagg
     8461 cagagtatga agatcaggtt cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg
     8521 gtatgaccac ggaacaactc cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg
     8581 ccgtatgacg tggaatggaa gcagggagcc atcgaggatg taacgacgct gtttgactat
     8641 atcgcggaaa attcatccct gtgggatgcg cagaacgtta ccgaccgggt tctcgctgcg
     8701 gcggacaaac tggccgactt cccgcgactc tatgaagtgg actcacgcta tggcgaggac
     8761 gttcgccgga tcagtctgat gggacagcat gtcctctatg atgttgatga ccagaccaga
     8821 aaagtccggg ttctggccgt ggtcggccag cgccagaatc cccatattgt tctctaataa
     8881 ccggtgacgg ggacgcaatt cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc
     8941 agcgttttcc ccacggccac caccggcgcg gtgttccggc cggggtctct gctgtctccg
     9001 gtttgtgttc cagcagtctc atggactgca tttcctgccg tagcccggcc acctcctccc
     9061 tgagtgcttt tatctccgcg atcaacgcgt cggaggccat tttttcagtc tcacccgaca
     9121 gtctcaactc gtcccgctcg tgagtctcat cctgtcttaa ctcaccgtac acccggataa
     9181 gctcgctggt atccaccacc cggccgccat cagcttccgt acggtatgat acgcggcctt
     9241 tcgccatatc ccgatacagg gaacttcggg ctttacccgt cagttccgtc gcct
//
