LOCUS       CP052234                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0033 plasmid pE17KP0033-3,
            complete sequence.
ACCESSION   CP052234
VERSION     CP052234.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609946
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:34:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,701
            CDSs (total)                      :: 5,579
            Genes (coding)                    :: 5,424
            CDSs (with protein)               :: 5,424
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 77 of 155
            Pseudo Genes (incomplete)         :: 77 of 155
            Pseudo Genes (internal stop)      :: 28 of 155
            Pseudo Genes (multiple problems)  :: 24 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0033"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE17KP0033-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(8205..9294,1..875)
                     /locus_tag="HJX58_28455"
     CDS             join(8205..9294,1..875)
                     /locus_tag="HJX58_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ73015.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            875..1606
                     /locus_tag="HJX58_28460"
     CDS             875..1606
                     /locus_tag="HJX58_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ73016.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            1613..2143
                     /locus_tag="HJX58_28465"
     CDS             1613..2143
                     /locus_tag="HJX58_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ73017.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2171..2350)
                     /locus_tag="HJX58_28470"
     CDS             complement(2171..2350)
                     /locus_tag="HJX58_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ73018.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2419..2796
                     /locus_tag="HJX58_28475"
     CDS             2419..2796
                     /locus_tag="HJX58_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ73019.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2858..3157)
                     /locus_tag="HJX58_28480"
     CDS             complement(2858..3157)
                     /locus_tag="HJX58_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ73020.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3147..3410)
                     /locus_tag="HJX58_28485"
     CDS             complement(3147..3410)
                     /locus_tag="HJX58_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ73021.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(3716..4135)
                     /gene="exc2"
                     /locus_tag="HJX58_28490"
     CDS             complement(3716..4135)
                     /gene="exc2"
                     /locus_tag="HJX58_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ73022.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4538..6223
                     /locus_tag="HJX58_28495"
     CDS             4538..6223
                     /locus_tag="HJX58_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ73023.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            6233..6490
                     /locus_tag="HJX58_28500"
     CDS             6233..6490
                     /locus_tag="HJX58_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ73024.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            6575..6724
                     /locus_tag="HJX58_28505"
     CDS             6575..6724
                     /locus_tag="HJX58_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ73025.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6828..7406)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          876..1603
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agctgattgc ggagcagttt ccgcaggggg caaacattca gcaggtgctg gcggcggcgt
       61 acgcgctgcc ggaggccctg aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg
      121 tcgctgacct gagcgtcctg cagacggaca gtggcatcga tgtggcgggg attatcaacg
      181 gcggcggctg cctgtacgtt atcggctcga tggacgacga ggcggtgatc cgtgtgcaaa
      241 aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggacgagttc cgggagtggc
      301 cacacgtcag cgtgatgctc gatgaaatta aatatctgct gtcgaagtat gtactgaacg
      361 cactggggac gctgcgcagc cgggactgca atctgttgct cgcgcaccag tcgctgggcg
      421 atttcgggca gtgcgggcag gatctgcccg ctgaatttgt gaaaaccacg gtactggata
      481 acacgccgat ccgctggttt taccgggcgg ccagcctgga gtccgcgcag tgggcggcgg
      541 ggcagaccgg ggaaatcagg gtggacgtgg agcgccgccg tgccagccgg gaggcgggga
      601 acgtggagca tatcagcggg gacagcttca tccagaagga ggcgcggccg ctgtttgacg
      661 tgaacaccct gcaacacctg ccggacgggt tcgcggtcat gaccgggctg ggtgtggccc
      721 ggatggcgtt cagcagtccg ctgcgggttg agcgccggga aatcccgttg cagcgtttcc
      781 cggtgctggc gaaaaccgat cccctggcgg agttccggcc cccggccgac ggcggcgggc
      841 cgaaagcgga tgatgatttt gcggggattt actgatggcg ctggagctgt acaacgtcag
      901 ccacgcgaaa cgacaggcgc ggaacgcgga gaaaacccgg ctgaccctgc gctggttgcg
      961 ggaagagctg tgttccacgg cggagctggt tgcgcggcgg ctggggattg cggcggtgca
     1021 gccggtctac cgcttcctcg acagcctggt ggcgaaaggg ctgctggtcc ggaccaaata
     1081 tccggtggac ggccgtcagg tcagcgtgtg ggggctgacg ccgcatggcg tggcgttctc
     1141 gttcgacgag gacgaaccgc tggcggatgt tattccgttt cagccgtcaa gggtgtcggc
     1201 agcacatctg ccgcaccggc tggccgtgca atccctgcgc cttgatatgg aagccagagg
     1261 ggccaccggc tggcgcattc tgcaccgtct gagtcttaaa ggcatgaaag tgccggacgc
     1321 cctggcggag ctggacggta aaacggtggc gtttgaggtg gagcgcaccg tcaagagccg
     1381 tcgccggtat caggaagtgg tgtccgggta tctgttcaac cgcaaagcta acggcatcga
     1441 tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc agggtgcagc gggcaatcct
     1501 gtcggtggat gaaatcgtga acccacagac cggcgaggcc cgcaaaacgg cagagctgga
     1561 tcgtgaacgg ttgtttgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct
     1621 gtacatggta tttattgaag acgcggagga ccggaacggc gagcgctgca tcatgccgac
     1681 cagcgagcat ctggaagata tggtggtgct cgcctggcag accggggcgt atgttttctg
     1741 ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg
     1801 gctcaacagc gacgaaaaag ccacgtactt tttcactggt aaaaatccgc aatgggaacg
     1861 gaacaaaccc acaggaatga agctgtgtga ttacctctat cgcaaggtgg agcggttcgc
     1921 catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc
     1981 gatgattgac gtggtgagtg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc
     2041 ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtacctg aactgccgga
     2101 ggacaaacct gcccgccggt ggtggccgtt cggcaaaaaa taaatgccag caccggccat
     2161 gccggtgctg ttattcctcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat
     2221 cctcgtgtag ccgctcgcac atgtcggccg gggtatccat atccagctca ttcagtttat
     2281 cgagcaatag cagcgactgc tccttaataa atttagccat attgagggct gttttttcct
     2341 gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc tgtcaggtat actgtctcaa
     2401 aaatagcggg gtgagacaat ggcgtgggtg acggtgagac aggcgacgga actgacgggt
     2461 aaagcccgaa gttccctgta tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa
     2521 gctgatggcg gccgggtggt ggataccagc gagcttatcc gggtgtacgg tgagttaaga
     2581 caggatgaga ctcacgagcg ggacgagttg agactgtcgg gtgagactga aaaaatggcc
     2641 tccgacgcgt tgatcgcgga gataaaagca ctcagggagg aggtggccgg gctacggcag
     2701 gaaatgcagt ccatgagact gctggaacac aaaccggaga cagcagagac cccggccgga
     2761 acaccgcgcc ggtggtggcc gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg
     2821 acggccacgc aatgaattgc gtccccgtca ccggttatta gagaacaata tggggattct
     2881 ggcgctggcc gaccacggcc agaacccgga cttttctggt ctggtcatca acatcataga
     2941 ggacatgctg tcccatcaga ctgatccggc gaacgtcctc gccatagcgt gagtccactt
     3001 catagagtcg cgggaagtcg gccagtttgt ccgccgcagc gagaacccgg tcggtaacgt
     3061 tctgcgcatc ccacagggat gaattttccg cgatatagtc aaacagcgtc gttacatcct
     3121 cgatggctcc ctgcttccat tccacgtcat acggcatcgc gcagttcctg ataacggcca
     3181 tgcaggcgct ggcggagttg ttccgtggtc atacccggac gcgtgtcagc acgggcggcg
     3241 gtcactttgg cgcgaacctg atcttcatac tctgcctggg ccagttcttc gttagccagt
     3301 cgctgcaaca gcgcagcttt gtccgggcca ccttccggca gtatgcgggg caggctttca
     3361 gccagttccc gaagaaagtt ggcgttttcg tagtgtgtag tgttgctcat tgcggagcct
     3421 cccgggttaa tgcggcaata tgaaggaagt ttagcagggg gcggtctgaa agaatatggt
     3481 cgtgtcagtc aggctgatga agcagaaagt cggtaaaaaa gggcttacgg catccatttt
     3541 acgtcaaaac atatgccatg cagttgcgtg cataggcatg cattaggata aaatttatcg
     3601 ggcgcgtttc cggcagtttt ccgggggggt tgttgcctgt tttatcccgt agccgccgga
     3661 aacgccctga gcccgtctga gcggtgcgcg taatgacgcg ttatggtaaa tccacttact
     3721 gtctgccttc atagccatcg agataaacag ctgaaatggt gtcagccagt agccttgatt
     3781 ttttcggtgt gaggtctttg ctgcggtaat cggtacccgc aaattgtgtt gtgccttgga
     3841 gtgagtttaa aaattcctga ctgtgaaact gctgaatgcg ttgctgcaca tcactccgtg
     3901 attttccggt cgctttatcc tttgcgccca tatcccagaa ctgccggaaa aaaggcacca
     3961 tcattcgtgt tgtgtcagct ttctgggtgg aaaaattggg atcaaaaccg tcatctgaag
     4021 catatacgta atgccgggta tgctgttcca cgctgccctt tggagcacag gcagtgagag
     4081 ttgctgtcgc tgttatcatg gcgataagac acagtttgct gccggaacgt ctcatgagcg
     4141 tctccttacc tttaattctt ttttctcatt gagttatata tccgcaggct atcttatcat
     4201 cagtttctga ccgtgttcag tgaccgaaca acccgaacca gaccccccgt ttcttttcct
     4261 gtcccggagg gggctttctg gtatctcata taatgatttc agtaaaacat actgaggccg
     4321 gagaacggcg ccgtttgtgc tggtttttgt tgcatctgga ggggattgcg catggtgtga
     4381 gtggctgttt ttttgatcat tttcacaaaa acgcaaattt acagacttga catgaaaaac
     4441 aaggcagcgt aatttttata ctgtatataa aaccagtggt tatatgtaca gtattttatt
     4501 ttagtttatt gttttaaaag tcaaagagga aaagtatatg agtggcggag acggacgagg
     4561 tccgggtaat tcaggtctgg gacacaatgg tggtcaggcc agtgggaatg tgaacggtac
     4621 atcaggtaaa ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg
     4681 gggtacgacg catacaccta acggggatat tcataactat aacccggggg agtttggtaa
     4741 cggcgggagt aaacccggtg gtaatggcgg taacagcggc aatcatagcg ggagctctgg
     4801 tggcggacag tcttcggcca ccgcgatggc cttcggtctg cctgccctgg ccactccggg
     4861 ggctgaagga ctggctttat ccgtttccgg cgatgcgttg tcggccgctg ttgctgatgt
     4921 gctggctgcc ctgaaaggtc cgtttaagtt tggtctgtgg gggattgcga tttacggagt
     4981 gctgccttct gagatagcaa aagatgatcc gaaaatgatg tcaaaaatcg tgacgtcatt
     5041 gccggcagac acggtaacgg agacgccggt cagctccctg ccgctggacc aggcgacggt
     5101 cagcgtcact aaacgtgtgg cggatattgt gaaggacgag cggcagcata ttgcggttgt
     5161 caccggccgg ccaatgagtg ttcctgtggt ggatgcgaaa ccgacaaaac gtccgggggt
     5221 attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg agcgtaccta aaggtgttcc
     5281 ggccgcgaaa gccccgccaa aaggcattat tgctgaaaaa ggtgattcac gtccggctgg
     5341 ttttacggcc ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag agagcggaca
     5401 gaaaccggtc tatgtgtcgg tgacggatgt tcttactccg gcacaggtaa aacagcgtct
     5461 ggaggaagaa aagcgtcgcc agcaggcatg ggacgctgct cacccggaag aggggctgaa
     5521 aagagagtat gataaagcga aagctgagct ggatgctgaa gataaaaata ttgcgacctt
     5581 aaacagtcgc attgcatcga cagagaaggc gatccccggt gcaagggctg ctgttcagga
     5641 agccgataaa aaggtaaaag aggcagaggc aaataaagat gattttgtga cttataaccc
     5701 gcctcatgaa tatggctccg ggtggcagga tcaggttcgc tatcttgata aggatattca
     5761 gaatcagaat gagaaattaa aagctgccca gacatcttta aacgaaatga atgaatcctt
     5821 atccagggat aaggctgcgc tttccggggc gatggagagc cggaaacaaa aggagaaaaa
     5881 agcgaaggat gcagaaaata aattaaatga ggaaaagaaa aaacctcgta agggaactaa
     5941 agattacggc catgattatt ttcctgatcc caagactgaa gatattaagg ggttgggaga
     6001 gttgaaagag ggtaaaccta aaacccctaa acaaggtggt ggtggtaagc gggcccgatg
     6061 gtatggtgat aaaaagcgta aaatttatga atgggattcc cagcacggtg agcttgaagg
     6121 gtaccgcgcc agtgatggcg aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt
     6181 taaagggccg gatccaaaac gaaacattaa aaaatatctt taagaggtaa atatggggct
     6241 taaattacat attcattggt ttgataagaa aaccgaagag tttaaaggtg gtgaatactc
     6301 aaaagacttc ggtgatgatg gttctgtcat tgaaagtctg gggatgcctt taaaggataa
     6361 tattaataat ggttggtttg atgttgaaaa atcatgggtt tcgatattac agccacactt
     6421 taaaaatgta atcgatatta gtaaatttga ttactttgta tcctttgttt accgggatgg
     6481 taactggtaa gcaaaagtta tcaaggatga tttgaaatac agacttccgg tgttcaggga
     6541 tgaataccgg ggtttttaaa tacagggaag gtatatgaaa aaggtaaagg ttgtttttct
     6601 ttttatattg attttttcag gctttcttct ggtcgcatgt caggcaaact atatccggga
     6661 tgttcagggt ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga tcgcggttca
     6721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct
     6781 gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact
     6841 gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta
     6901 gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg
     6961 aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgcttttcc gggttggact
     7021 caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggggt tcttgcttac
     7081 agcccagctt ggagcgaacg acctacaccg agccgagata ccagagtgtg agctatgaga
     7141 aagcgccaca cttcccgcaa gggagaaagg cggaacaggt atccgggaaa cggcagggtt
     7201 ggaacaggag agcgcaagag ggagcgattc atcggaaacg gtgatgatct ttaagtcctg
     7261 tcgggtttcg ccactcctga ctgattcatg gttgagccac cggctcccac agatgcaccg
     7321 aaaaagcgtc tgtttatgtg aactcagtca ggagggcgga gcctatggaa aaacgccacc
     7381 tgcgtggcct tgccgttttg cctcacatgt tagtcccctg cttattcacg gaatctgtgg
     7441 gtaactttgc atgtgtccgc agcgcccgcc gcagtctcac ggccggagcg tagcgaccga
     7501 gtgagcgagg aggcgcaatt tccccggcac ctacaagacg gacaccggac gcgctgatgt
     7561 ggcatattgt cccctgatta agtctttata ttccctgata ataaattacc aggataggtg
     7621 aatcatatcg gtttttcgct gaacatttta cgttaaccta tctctgttgc ttttttctcc
     7681 ttacggagaa atacaggtca tccccgcgag ggcgtggctg acctcagggg gatatcccct
     7741 gcttacgctg gctggacgcc accgacgcac ggtctctccc cctctttctg gctccgtccg
     7801 gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc
     7861 cacaaaaatc cacggtgctg gacgcacctg gcgaagcagc aaagctgctc ctgattttgc
     7921 gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg
     7981 cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct
     8041 gcgccttttc cgcatggggc ggaaagaggc tggcctccgt ggtgaaagct gaacgcaatc
     8101 accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg
     8161 gctgcacatc acgccggcaa accatcgtct gaaggagaga gaacatgttt aatacggatt
     8221 cgcttgcctg gaagtggcac attccggagc tggcccggca gatttatccg tgggtcagct
     8281 ggctgtacca ggagtattac ggtctggccg gggctttcct agttatcggc ctgtttttcg
     8341 gtctggctga actttgggcc ggacggatgc gttttgtggg gctgagtcat aaggagcgac
     8401 gggagtatgc cgatcgctat aagcgcgata ataaacggcc agcgccattt tcggtgctgg
     8461 cctcgctgga ttcgcccggg cggatggtca gggcacgtcg acatgtctgg aactacgcgt
     8521 actggctgac cggcgcggcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta
     8581 tcgagagctg gggagaaacc gtcctgcgtc cggacctcta cgcaaacgca gattattacc
     8641 tgctgcagcc ggctaacctg ttcgggattg cctggtgcgc gggcgggctg gctgtcggga
     8701 ttgtgttggc gcggctgctg gcgttcctgc tgcttgacgg tcattttgcg gctgctgacg
     8761 aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg acaggagaga
     8821 tgaccgacgt gcgccacctg catttcgggg agccggtacc ggttaatgcg ctggccgatt
     8881 tttcctcgga gcaggcccgg caggagcagg cggtgtttct gggtaaggat gagcagggca
     8941 agccggtgct ggttccgcgt gatacctggc gcaagaccaa cattcagatc ctcggcctgc
     9001 ccgggagcgg caagagcgtg atggccacca acgcgctcat tcgctgcgta cgcgatttcg
     9061 gggacgcggt ggtgtacttc gaccccaaag gggacgcatg ggcgccgcac gtcttccggg
     9121 cgcactgtcc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc
     9181 tgttccgcga tctcgaccag tacgcgctga aaaacctgct gattgccggg cttaacctgg
     9241 gcgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcga
//
