LOCUS       CP052223                3288 bp    DNA     linear   BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0053 plasmid unnamed.
ACCESSION   CP052223
VERSION     CP052223.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609948
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:33:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,499
            CDSs (with protein)               :: 5,499
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 65 of 147
            Pseudo Genes (incomplete)         :: 77 of 147
            Pseudo Genes (internal stop)      :: 26 of 147
            Pseudo Genes (multiple problems)  :: 21 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0053"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(423..974)
                     /locus_tag="HJX60_28815"
     CDS             complement(423..974)
                     /locus_tag="HJX60_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO86683.1"
                     /translation="MVLELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTV
                     QRTINKLISKGFLKRKIRAKSVNDKGLSNLYSFEPLIEKLLISISNNEDQYEELQKEQG
                     ERDLKTVDQLVRIAAAGGGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKT
                     VDELVRIAAAGKGCVLFELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            1292..1522
                     /locus_tag="HJX60_28820"
     CDS             1292..1522
                     /locus_tag="HJX60_28820"
                     /inference="COORDINATES: protein motif:HMM:NF015947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="QJO86679.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1519..1857
                     /gene="mazF"
                     /locus_tag="HJX60_28825"
     CDS             1519..1857
                     /gene="mazF"
                     /locus_tag="HJX60_28825"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="QJO86680.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(1988..2121)
                     /locus_tag="HJX60_28830"
                     /pseudo=""
     CDS             complement(1988..2121)
                     /locus_tag="HJX60_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2146..2570)
                     /locus_tag="HJX60_28835"
                     /pseudo=""
     CDS             complement(2146..2570)
                     /locus_tag="HJX60_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            2527..2718
                     /locus_tag="HJX60_28840"
     CDS             2527..2718
                     /locus_tag="HJX60_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO86681.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2718..3119
                     /locus_tag="HJX60_28845"
     CDS             2718..3119
                     /locus_tag="HJX60_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QJO86682.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     Typing          complement(2021..2479)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.526"
                     /coverage="90.0"
                     /evalue="1.69e-147"
ORIGIN
        1 gaatcgccgg tgaatcgtgt gctaagtcgt ccggtgactt gccatcacgt cattgccggc
       61 tgtcgctgaa ctggattctt gaacgtgctg aaaacgggtt ttccggctgc gccggcaacg
      121 cctcacggcc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatcag aaaaacgccg
      181 gaaacaggac ttaccctggc tgaatggcat gaatgcccgc gctatgcact tgcatgcata
      241 ctcatgtatg ccgtaaaaac agcccctgcg cgtttctggc aggttttcgg gtggtttgtt
      301 gcctgttttg ccggtttccc gtcagaaacg ccctgaagcc gttttagcgg tgcacgtaat
      361 agggctttat gttaaattaa gactggtttg atgtttaaac ttatagtgat ctttttagtg
      421 tgttactcca attcgaatag tacacacccc tttcctgctg cagcaattcg aactagctca
      481 tcaacagtct tggactcaag atcaaacaga actaactttg ctttattggg ggttatcgag
      541 ttacctgcgg ctgcggcaat ccgcacgagc tgatccactg tttttgtgga tgcacttaaa
      601 cgtaaaccac ctcctgctgc agcaatccga actaattgat ctactgtttt caagtctctt
      661 tctccttgtt ctttctgaag ttcttcatac tggtcttcgt tgttactaat tgatattaat
      721 agcttttcta tcaaaggttc aaagctgtaa agattgctca agcctttgtc attgactgat
      781 tttgcgcgga tttttctctt caagaaccct ttagatatta atttgtttat ggttcgctgc
      841 acggttcttg ggtgttggtt gatcctacct gctagtgtag aaatgcttgg gaatgctggg
      901 gtgtctgcaa agtgaatgaa agagattaga tgaatgagta acagcatttc ggtgttactc
      961 aattctagaa ccatctgttt ttcaagcaat aaattaggaa cagttgtcca ccctgcttcc
     1021 tctaggatgc tatatcccca cctgcttgct aagatcgctg gtatttggtt gcggtttatt
     1081 tttttttctt tgtaaatcaa tttgttatcg ccacttagtt aaaaaaaaat tttttttgcc
     1141 attggtaact acttaaacaa ataagtattt atttgtttaa gtagttaccc ctttttaggg
     1201 tggcattggt gccacccttc aaactgaact caaagactat acaatacaaa tattgtttac
     1261 acattgttta tccatctgct ttggaggtat tatgactacg gctattggta aatggggtaa
     1321 tggcgctgct ttgcgtatcc cgcagccgtt catgaagcag cttgggctta gtatcggtga
     1381 tgttgttaat ttccgcgtca ctgaaaggga tttagtaatt actaaagctg gcccgacgct
     1441 tgaagaactg ctcggacagt gcactgcaga aaatcgccat cacgagtatc tatgtgactc
     1501 tcaaggtaag gagatgctgt gacgacatat tgtccagcca gaggcgatgt cattctttta
     1561 gattttaacc ctcaatcagg gcatgagcag gcagggaaaa ggcctgctct tgtcgtttct
     1621 gatgatttgt ttaaccaagt cactgggttt gctgttgttt gccccataac taatcagata
     1681 aaagggtacc cttttgaagt tcctgttgat gggactacta aaacaacagg ggttatcttg
     1741 gctgatcaag tgaagtcttt agactggaag gccagagcgg cacgtactgt tgatagcgtt
     1801 tccggcgaga cagtaactac agttgttgat atggtttcaa aaattattaa agcataaaaa
     1861 aagaccggca taagccggtc ttttcaggcc gaaaatgtta ccagcatttt cggccttcgg
     1921 tgactgctct aaaggggaac agtaaccaat attgtttatc aagtgtaact gcttactgtt
     1981 acagcaatca agattttctt tcccccgtag agcgaccgat ggctactgcc aatggcgctt
     2041 tggcgtgctt gttcgggttg gactcaagtt gatagttacc gaattagcgc agtagtcgga
     2101 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctaac cggaactgag
     2161 tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     2221 aggcgggaac aggagagcgc acgagggagc catcaggggg aaacgcctgg tatctttata
     2281 gtcctgtcgg gtttcgccac cactgatttg agcgtcaaat tttgtgatgc ttgtcagggg
     2341 gggcggagcc tatggaaaaa cggccgttgt gcggccttgt ccctcttcgt gctgtctgtc
     2401 ctatgccgtt atgtgtgcgt tgcgaccgct cgccgcagcc gaacggcagg agcgtagcga
     2461 ctgagtgagc gaggaagcgg aaaagagact ggttttacac tgagcactga cgctctgagg
     2521 cttcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat ccagcatcag
     2581 tctctgctcc tgctggagaa gcttaacgag cttgatcttg atgctgaggc cgacctttgt
     2641 gagaaactcc atgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgca
     2701 ttacaggatg gtaactgatg gccagagtgt ctattagtga ggcagcccga ttgacgggta
     2761 agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc tgtagcggtg
     2821 aacgtaatgc aaaaatggta gatacgtcag agcttttgcg tgtctttggt tcctttgagc
     2881 aacctaaggg tgaacatctt tctggacagg tctctgaaca gcgtgataca gcctttgctg
     2941 accagaatga acaggttatt cgtcatctga aacaggaaat tgaacacctg aacaccttac
     3001 ttttgtccaa ggattcgcac attgacagcc tgaaacaggc catgctcctt cttgaaaatc
     3061 accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa aaatcctgac
     3121 accgtcatgc ttcagttttc ctctccggcc attccgggga gttcctgaac cttcccaaaa
     3181 atcgtcagat ctctgaacgc acctcaccac ttaaaatacc cacacaccac gcaaaattaa
     3241 aattttgcag ataactcatt aatatcagtt tgttaggtgt tattaaat
//
