LOCUS       CP052221                5219 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0053 plasmid pE17KP0053-4,
            complete sequence.
ACCESSION   CP052221
VERSION     CP052221.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609948
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:33:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,499
            CDSs (with protein)               :: 5,499
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 65 of 147
            Pseudo Genes (incomplete)         :: 77 of 147
            Pseudo Genes (internal stop)      :: 26 of 147
            Pseudo Genes (multiple problems)  :: 21 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0053"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE17KP0053-4"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(4876..5219,1..415)
                     /locus_tag="HJX60_28740"
                     /pseudo=""
     CDS             join(4876..5219,1..415)
                     /locus_tag="HJX60_28740"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            393..923
                     /locus_tag="HJX60_28745"
     CDS             393..923
                     /locus_tag="HJX60_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJO86665.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTAWTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="mobilization protein"
     gene            930..1163
                     /gene="mbeD"
                     /locus_tag="HJX60_28750"
     CDS             930..1163
                     /gene="mbeD"
                     /locus_tag="HJX60_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJO86666.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(1708..2004)
                     /locus_tag="HJX60_28755"
     CDS             complement(1708..2004)
                     /locus_tag="HJX60_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO86667.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            2216..2413
                     /locus_tag="HJX60_28760"
     CDS             2216..2413
                     /locus_tag="HJX60_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO86668.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            2431..2715
                     /locus_tag="HJX60_28765"
     CDS             2431..2715
                     /locus_tag="HJX60_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO86669.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="hypothetical protein"
     gene            complement(2779..3168)
                     /locus_tag="HJX60_28770"
     CDS             complement(2779..3168)
                     /locus_tag="HJX60_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO86670.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            4090..4269
                     /locus_tag="HJX60_28775"
     CDS             4090..4269
                     /locus_tag="HJX60_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO86671.1"
                     /translation="MSEEAEKRLDVHFLLTHLCGISHPVWRTFHTIRTDAAWLSQYTLR
                     YRYVTGSGLRPDTR"
                     /name="hypothetical protein"
     gene            complement(4355..4546)
                     /locus_tag="HJX60_28780"
     CDS             complement(4355..4546)
                     /locus_tag="HJX60_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO86672.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4611..4934
                     /locus_tag="HJX60_28785"
     CDS             4611..4934
                     /locus_tag="HJX60_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJO86673.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4184..4270
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.47e-38"
     Typing          complement(3616..4172)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.462"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1267
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.052"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4579..4931
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.305"
                     /coverage="100.0"
                     /evalue="5.85e-70"
ORIGIN
        1 caagggacgg cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg
       61 cctccagccg tactacgacc gggcagacag gccgagaatt gatgcctggc agacgctggt
      121 gaatcaccat tacgggctgc atgaccccaa cgccccggag aaccgccgga tactgactct
      181 ctctgataac ctgccggaaa cgaaacaggc gcttgcagag agcgtcacac gcgggataga
      241 cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg cgctcacaga
      301 ggcagggctt aaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg
      361 tgggaagaat atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgcgg
      421 agttcgagaa aaagctgaaa gagagagccg aatctacaga gacaatactg aacgacgagt
      481 tcaggaagct cgaaaaatct gtaagcaagg ctgtgacatc aaacgagacg aaaatcaaag
      541 acgctatagc cctgttcaca gcttggacag aggaatcact gaaaaaacac cgggaagggg
      601 tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca
      661 tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg ctctggtatc
      721 ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag aatctggaga
      781 agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga cgttttctgg
      841 tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg aaacagaatg
      901 ccgtaaggct ggtacaggag taacagagca tgacggagct ggaaagacat ttgctgaacg
      961 cattagagca actgcaacag gactatatgc agcggctgaa cgaatgggag agcgccttcg
     1021 tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg
     1081 tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc ttgagccagt
     1141 tatacagcga gaacaggaga taagggacga acggctgata caggaacgcg aacatgcgtt
     1201 atccctggag cgtgagcgcc agctggaaat acaggaacgg acactggatg gcccttcgct
     1261 gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc attttttggg
     1321 ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct gaaaacacag
     1381 gaacgcgtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgttgct gtgttttgcc
     1441 tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctccg gtccccgtct
     1501 tcttcgcttc cgaaagcatg aaaaaaatcc gtttcagctg tctctggctg gcaattcctg
     1561 catgattcac atagctgcat agctatgcat gtggttaaaa tttaccgggt gcgatcgcgg
     1621 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgccgaacg
     1681 cgtttcagcg gtgcgtacaa taagtgatta tggtaaatca cccttccagg cgttcagccc
     1741 ttcttccggt ttttttccgt tcaggaattt acgggcgaga cgaatgcgtc ttgcctggta
     1801 aatcgcatcg taggcctgtg actgtcccac accgttactg atttcactga ccagatggcg
     1861 caggtcggcc tgtgtcagtg tgtaaacgtg tttctggatg aggtcacggg ctgtccgctc
     1921 ttcgtatccg gcagcgataa gtgcagccag caggcgtttg tctgttttgc tcagtcggtg
     1981 gcgaccagat tttgttttgc tcacgttatc tcctctctgt tttttctcct gatgttatcc
     2041 ggtgctgttt ttctgctgcg aatcttccct tcctgatttt tataagggtg attcagatca
     2101 caaaaatgaa aattcatatc attgacagga aaaataatgc ggcgtaattt atactgtata
     2161 taaaactagt gtttatatat acagtatatt ctgtgtaaat aaagggaggg ataaaatgaa
     2221 agaagaaaaa gtattgcttc accgtttctt atttgtagtt aggaataaga atggttgtga
     2281 actatcatgt tcggcagacc ttatgggaac aagggatgat gtttataagt atttctctga
     2341 ttctgttagt gggctagatg ttgagcttat agatgtttct tgcgagagtg aatgggaaga
     2401 acattcgcat tgaactaatc aggagattat atgatttctc aaaatgtttg ggctcctcct
     2461 ttcttttttg gtccttgggt tgaacttgaa aatcatactg gtgatatcgc gtacactgtc
     2521 tattttgata cattgtctaa tgtacccggt ccatttattg cttttatctg gtatgcccct
     2581 aatgggccaa gaacagttaa cgttattggt ccagcatctt atcagatctc ttgcaagggc
     2641 gctggtgttg ataaaattaa atttaaaagt gtcagtggga cagggcagat tattcatgta
     2701 tacactacct tataataaga gctgaataga tataatcctg cctaagctat accttaagca
     2761 ggattatatc tattaatatt attttgaata tgcttttatg atttccatca ctgttgtttc
     2821 atcgttcaca ggttgataat aatgggttgg atttagtgca gaatgaatga ttgcaggttg
     2881 ggatattact tttccatcaa aatatgaaat caggggtttc cctatatttt tactagttat
     2941 ttcgtttagt tttttcttac ataaactatc tgtgtttact tctattatga cgcctttgtt
     3001 tttttctgat tgggaagggt acattttaag tatgcaatat ttattcagaa tgatgctgtc
     3061 accaccaact ttgtaaacaa tgtttttgtt tgtttcagta cttgaacatc ctataagtaa
     3121 ggtcagtata gtactcgatg ttattgactt tactgatgat ttgaacatat taactccatt
     3181 ttattttgtt aaaattccaa tctataaata catatacacg taaaaataaa caatgcgaac
     3241 gttttcactt atattatccc catgatgtga ttgaatggtg gttccatata agagagggca
     3301 attatactat ctttctctcc tgaactgatg ggattgcagt ttagagaaaa aagatcaaag
     3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg aaaaaaccac
     3421 cctggcaggt ggtttttcga aggttagcta atcctggcag attacctaac cgggtagctg
     3481 gcttaagtgg agcacagata ccaaaagatg tcctttcagt gtagccgcta taaggcttca
     3541 acttcaagag ttctgcgtat ctaaatccac tttttagtta ccaatggctg ctgccagtgg
     3601 cgttaagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3781 ggacaggtat ccggtaaacg gtaaggtcgg aacaggagag cgcacgaggg agcttccggg
     3841 gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg cgcggccttc
     3961 ttcctgttcc tggtcttttg ctcacatgtt ttttccggtg ttatcccctg attctgtgga
     4021 taacaccatt tcgtttttga gtgagtagat accgctcgcc gcagtcgaac gaccgagcgt
     4081 agcgagtcag tgagcgagga agcggaaaag cggctggacg tgcattttct ccttacgcat
     4141 ctgtgcggca tttcacaccc ggtatggcgc acttttcata ccatccgcac tgatgccgca
     4201 tggttaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct gcgccccgac
     4261 acccgctaaa acccgctgac gtgccctgac gggcttgtca gctcccggca tccgctcaca
     4321 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatccccga
     4381 agcgagcgag gcaactgcgg taaagctcat cggcgtggtc gtgaagcgat tcacagatat
     4441 ctgcctgttc atccgcgtcc agttcgttga gcttctccag cagcgttaat gtctggcttc
     4501 ttataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     4561 aagggggatt tctgttcatg gggataatga taccgatgaa acgcgagagg atgctcacaa
     4621 tacgggttac tgatgatgaa catgcccggt tacttgaacg ctgtgagggt aaacagctgg
     4681 cggtatggat gcggcgggtc tgcctgggtg agccggttgc ccgttccggg aggctgccga
     4741 cactggcacc gccgttactg cgtcagctgg ccgccatcgg gaataacctg aatcagacag
     4801 cgaggaaggt gaacagcggg cagtggtctt ccggtgaccg ggttcaggtg gtggccgcac
     4861 tgatggccat cggggatgag ctgcgccggc tgcgtctggc cgtcagggaa caggggacgc
     4921 gggatgatag ttaagtttca tgcccgggga aaaggtggtg gcagtggtcc ggttgattac
     4981 ctgctgggaa gggagcgtaa ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa
     5041 gtacgggaac tcatcgatgc cacgccattt gcgaagaaat acacgtccgg tgtgctgtcg
     5101 ttcgcggaga aggagctgcc accgggagga cgtgaaaagg tgatggcgag ctttgagcgt
     5161 gtactgatgc ccggtctcga aaaaaatcag tacagcatcc tgtgggtgga gcaccagga
//
