LOCUS       CP052215                4976 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0079 plasmid pE17KP0079-2,
            complete sequence.
ACCESSION   CP052215
VERSION     CP052215.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609949
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,472
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,249
            CDSs (with protein)               :: 5,249
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 49 of 103
            Pseudo Genes (incomplete)         :: 46 of 103
            Pseudo Genes (internal stop)      :: 23 of 103
            Pseudo Genes (multiple problems)  :: 13 of 103
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0079"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE17KP0079-2"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4431..4976,1..357))
                     /locus_tag="HJX61_27295"
     CDS             complement(join(4431..4976,1..357))
                     /locus_tag="HJX61_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="QJO22113.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSAIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKKMVE"
                     /name="replicase"
     gene            complement(605..880)
                     /locus_tag="HJX61_27300"
     CDS             complement(605..880)
                     /locus_tag="HJX61_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="QJO22114.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(861..1106)
                     /locus_tag="HJX61_27305"
     CDS             complement(861..1106)
                     /locus_tag="HJX61_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="QJO22119.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            1343..1504
                     /locus_tag="HJX61_27310"
     CDS             1343..1504
                     /locus_tag="HJX61_27310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO22115.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(2281..2682)
                     /locus_tag="HJX61_27315"
     CDS             complement(2281..2682)
                     /locus_tag="HJX61_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QJO22116.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEQHSEQVFEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAAWWKFWKKS"
                     /name="DNA-binding protein"
     gene            3071..3685
                     /locus_tag="HJX61_27320"
     CDS             3071..3685
                     /locus_tag="HJX61_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="QJO22117.1"
                     /translation="MGKHKVFISYHHANDQYYKNAIEKMNEQHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDKYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGAYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(3722..4429)
                     /locus_tag="HJX61_27325"
     CDS             complement(3722..4429)
                     /locus_tag="HJX61_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO22118.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
ORIGIN
        1 ttttttacgt agcgcattct ccacggaggc ggcgtagcgt aacggtttta ttttgccgtc
       61 cggcgcggtg cgtatcgagt tttccagcgc gtacagcagg tgcgcgtgtc cgttttcggg
      121 gttagttatg gtcagcgtcg gtgcaggcgc gttgcggtcg ctccagtcga tggcggaacc
      181 ggcacggtca acgtcaaatc ccagccagaa catggcgtga ggctgattga actggatata
      241 tttcgccaga atagcgcgtt ccttaccggc gatacggaca ccaaagtgca gatcgtccga
      301 aaagtacggc ttgtgtggta agcgttcgtt gaaaagttcg agtgcggcgt tatgcaatgt
      361 gccctgcgct tatggcgggc tgttgtctgg cacggttcat gtgctaatat cctttcaagg
      421 tttcgaccta gccctggtta tcatgcgatc cgccaagata tgacatgatt tccggggctt
      481 tgttttttct gaggctaacg ttacaccgac tttgcctttg tgtcgatctg acagcgactt
      541 accctaacat ccgttgtacg ccttgcatga aaagcccgca tcaagcgggc tatatcgact
      601 tttgctattt gtcaccatag tgataacggg ctgcgtagat gatgatttct ccgttctgga
      661 ttgaataaac cagacggtgg ctctgatcaa tcctgcggga gtagagtgcc ggatctttgt
      721 ggtgccgcag ccgttcgggt tttcctatac ctgtcagcgg atcagcctcg atgctgagca
      781 acagttgccg gatacgctca aacttctttt tgtcagtatc acgccaatag ttaaggtcat
      841 cagcgccatt cctagtgaac ttaatattcc acttcgacaa attcgccgcg ctccgcctgt
      901 ttcattgact cattgatatg ctctgcgttc gccggattgc tgaacagata gacagtttcg
      961 attagcgcct catactctcc cgcatcgatc ataacaatat caggcgcatc acggcgcatg
     1021 acacgtaccg gttcagcatc atcagtgaca cgcttcatcg tctcggcaaa atgtttacgg
     1081 gcatcagtaa aattgattgc atgcatgttg ttaaccccca tttgttgggg cttagcatag
     1141 aacatgtaca caaataagta catatagata tttccctaac aacccacaac caaatcagct
     1201 caaaatgtga actcaatcga ataaccacaa aaacaacaag tcaatacatt gaagcataat
     1261 ttcaagcaat tacatttatg aagtgcggca caatttcgta ccgctaggag cttagatcct
     1321 aaaacgagca aaggaaaagt aaatggcaaa acattggtct gggcaagaat gtccaaaaac
     1381 aggtacttac ggtcaatatc atgataccga caactcatac gctggttctg aatatgatcg
     1441 ccatgtgagc aagggtgata agttccctcc gagcaaaaac aaccaccact tccaagaaaa
     1501 ataaaatatc ttcagaagtc taaactaaaa gccctctggg gctttttttg tacctgccat
     1561 tcacttgatt taccataaca tccgttgccc gcaccgcctc agaggccctc agagcgtttc
     1621 tgacgggaaa ccggcaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcacagg
     1681 ctctgttttt acggcatgca tgagtatgcg cacaagtgca tagcgcggaa attgacacca
     1741 ttcagccagg ggaagtcctg tttccggcgt tcttctcctc agaaaacggt gaacggggac
     1801 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttcgtcgacg
     1861 gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca ccggcgattc
     1921 gactcatgac ttgactctag agtcaccgcg gtcaaaaaaa cgccaaagcg acgattcgac
     1981 tctagagtca agtcgtcata agttactgat agtttggagt gagtcgtcga tgactctatg
     2041 acgagtcgtc ggcaagtcgt acacgaatcg ccgacgactt ggattgacct tatagaaggt
     2101 aatactgcgt aatttaataa caaccaacta actgatatta atgagttatc tgcaaaattt
     2161 taattttgcg tggggtgtgg gtatttttag tggtgaggag cgttcagaga tctggcggta
     2221 tctgggaagg ttcaggaacg ccccgggatg gccagagagg aaaactgaag catcgcggga
     2281 tcaggatttt ttccagaact tccaccatgc cgccggggat tcagttaatt tggcctggtg
     2341 attttcaaga aggagcatgg cttgtttcaa gctgtcaatg tgcgaatctt tggacaaaag
     2401 taaggtgttc aggtgttcaa tctcctgttt cagatgacga atcacttgtt cattctggtc
     2461 agcagaagct gtatcacgct gttcaaatac ctgttcagaa tgctgttcac ccttaggttg
     2521 ctcaaaggga ccaaaaacac gtaaaagctc tgaagtatct accatttttg catttcgttc
     2581 accgctacaa gtggacagct cacctgtttt gattaacctg tgcagcgttg tccggctctt
     2641 acccgtcagc cgtgcagcct cactaataga cactctggtc atcagttatc atcctttaat
     2701 gcattcagtc tcaaggaaac aggggccagc ttacgacata ccggtccaga agtgatgtgt
     2761 ttttcggaag ggcgatgaaa cacaggagtg accgtgcaaa atgcaggcaa cagatgactt
     2821 tccgcttcct cgcgcattcg ctcactacgt tcggtcatga gactgcggcg agcggtacgt
     2881 acgaatgaag tgaggttata gatctcttct caaggcgttt agggaagcaa agagccctcc
     2941 ctccctaaac cagtcatggt gagaccttgc tgaagggacc ctgaaaaaaa cagctctcta
     3001 agagagctgt tatctgatat aggttcaagt gcaaaataga gtgcacacaa acaaaaaaag
     3061 gacaataaaa atgggaaagc ataaggtatt cattagctac caccatgcga atgatcagta
     3121 ctacaaaaac gcaattgaaa aaatgaatga gcaacatgaa atattcgtga atcgctcagt
     3181 atctttaggt gatatagatg aagatgaagc tccacaaaaa ataagagaga taataagaga
     3241 taaatatctt agagatacat cagttcttat ccttcttgtt ggaactgaaa caaaaaacag
     3301 aaaacacgta gattgggaac tttactctag catgagagat agtaccatta acaaaaaatc
     3361 aggaatttta gtcgtcaatc tcccgtcaac aaatactacg tacattcgtt caacacatgg
     3421 aactaatgaa aaatcagaag ttcatcctac cgtaactgaa tggttttcta ttgataaacg
     3481 ctcaacttat gaggaaaggt tcccatatat gcctgcaaga ataatagaca acttcatgaa
     3541 gagcggggcg tatatatcag tggtcaactg gaatcaaata gaaaataacc cagaaacact
     3601 cagaaaatta atagacatga cttataacga tagagaaaaa gcaacgtatg atatgtcgag
     3661 agaaatgaga atgcataatt catagtacta tccaaggagt agccttggct actcctttaa
     3721 tttaccagtt ccacttatct gtattgattc caaacttagg cttcacatca ttaacaaaga
     3781 accggttaac atctttaaaa ccttttaaaa tacgctcata atcataatac tgcatttgta
     3841 ttttatttat gctggttgca tgaaatctca tgacatcacc gctaacccta tcaacaacct
     3901 catccttgtc tgatatgtac ttataccaac gcttgtagtt gttctggtag ttagttagaa
     3961 atctatttaa caccatgagc atctcattgc tgaaatcata aatctcacga tcttttttag
     4021 ggtttagtat tttcatttca tcacgaataa attggtaagt ctgataatat gatgaaataa
     4081 cctcatcaaa ggcatcaacg ttttttctta atttgaaaat tgctcttttt gaatctaggt
     4141 gattttttac actctttttg taaagtgtag cactctcatt gaaaagtata ttcactccac
     4201 caaggctaag tttctcaacc ttaaccgaat actttttaag taagtagagg gataccatca
     4261 gccaaataaa aacagatatc aaaagaatag aaagggcgcc agttacaaga ggtctattct
     4321 tactaaaaac tgtgtaaaca gacggaatgt aattagatat atcaacagta cacatcacgg
     4381 caataaatgc tagaaaaact atagtccaaa gagttacgaa ggttttcatc ttattccacc
     4441 atttttttct tgatataaaa atacttccgc ctgcttattc ccatagctac ccaaggtttt
     4501 atttcctcta ggcttccttt cctccttgac tgtcctgact ttttccctct ctttgattga
     4561 atttcagagg aatgagtaat atcaacaaat ttactgaact cttcaggtga aaatttattt
     4621 gatgtccatt tagcaatgct cttagctatc cctcttacct cattttcatc cagaggtcgt
     4681 gcaaattgca ggttgtatgc gcaagcgcgt tcgtagcagg cttgcagcca ctgctcatac
     4741 tcaggccagc cctgacggat ggcgcgatat gcccacttac gggttttgtc gaacaacgtg
     4801 cagttacggc ctaagccgta gtcggcaacg atttccttgt cattccctgt gttcagatcg
     4861 agaaagtcgg ccagccagtc cagagtgtag agcttcggct gccacacggc gattttccag
     4921 tggttgtggt tcggattttt gcagatgagc cccgaatatc cagcgtcagc ctctaa
//
