LOCUS       CP052211                3378 bp    DNA     linear   BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0085 plasmid unnamed.
ACCESSION   CP052211
VERSION     CP052211.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609950
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:27:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,614
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,375
            CDSs (with protein)               :: 5,375
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 58 of 116
            Pseudo Genes (incomplete)         :: 54 of 116
            Pseudo Genes (internal stop)      :: 19 of 116
            Pseudo Genes (multiple problems)  :: 12 of 116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0085"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(169..570)
                     /locus_tag="HJX62_28030"
     CDS             complement(169..570)
                     /locus_tag="HJX62_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QJO70223.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(570..761)
                     /locus_tag="HJX62_28035"
     CDS             complement(570..761)
                     /locus_tag="HJX62_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO70224.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            718..1142
                     /locus_tag="HJX62_28040"
                     /pseudo=""
     CDS             718..1142
                     /locus_tag="HJX62_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1167..1300
                     /locus_tag="HJX62_28045"
                     /pseudo=""
     CDS             1167..1300
                     /locus_tag="HJX62_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1431..1769)
                     /gene="mazF"
                     /locus_tag="HJX62_28050"
     CDS             complement(1431..1769)
                     /gene="mazF"
                     /locus_tag="HJX62_28050"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="QJO70225.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(1766..1996)
                     /locus_tag="HJX62_28055"
     CDS             complement(1766..1996)
                     /locus_tag="HJX62_28055"
                     /inference="COORDINATES: protein motif:HMM:NF015947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="QJO70226.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2314..2865
                     /locus_tag="HJX62_28060"
     CDS             2314..2865
                     /locus_tag="HJX62_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO70227.1"
                     /translation="MVLELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTV
                     QRTINKLISKGFLKRKIRAKSVNDKGLSNLYSFEPLIEKLLISISNNEDQYEELQKEQG
                     ERDLKTVDQLVRIAAAGGGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKT
                     VDELVRIAAAGKGCVLFELE"
                     /name="helix-turn-helix domain-containing protein"
     Typing          810..1268
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.526"
                     /coverage="90.0"
                     /evalue="1.69e-147"
ORIGIN
        1 tttaataaca cctaacaaac tgatattaat gagttatctg caaaatttta attttgcgtg
       61 gtgtgtgggt attttaagtg gtgaggtgcg ttcagagatc tgacgatttt tgggaaggtt
      121 caggaactcc ccggaatggc cggagaggaa aactgaagca tgacggtgtc aggatttttt
      181 ccagaatttc caccatggcg ccggggattc agttaatttt gcctggtgat tttcaagaag
      241 gagcatggcc tgtttcaggc tgtcaatgtg cgaatccttg gacaaaagta aggtgttcag
      301 gtgttcaatt tcctgtttca gatgacgaat aacctgttca ttctggtcag caaaggctgt
      361 atcacgctgt tcagagacct gtccagaaag atgttcaccc ttaggttgct caaaggaacc
      421 aaagacacgc aaaagctctg acgtatctac catttttgca ttacgttcac cgctacaggt
      481 ggacaggtca cctgctttaa ttaacctgtg cagtgttgtt ctactcttac ccgtcaatcg
      541 ggctgcctca ctaatagaca ctctggccat cagttaccat cctgtaatgc atccagtctc
      601 gaggacagtg tacggaaaag atgctccgca tcatcatgga gtttctcaca aaggtcggcc
      661 tcagcatcaa gatcaagctc gttaagcttc tccagcagga gcagagactg atgctggatg
      721 aatttagcca tgttcagcgc tgttttctgt tgtttcgtca taagaagcct cagagcgtca
      781 gtgctcagtg taaaaccagt ctcttttccg cttcctcgct cactcagtcg ctacgctcct
      841 gccgttcggc tgcggcgagc ggtcgcaacg cacacataac ggcataggac agacagcacg
      901 aagagggaca aggccgcaca acggccgttt ttccataggc tccgcccccc ctgacaagca
      961 tcacaaaatt tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     1021 ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cccgcctttc ggtttaccgg
     1081 tgtcattccg ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccggtt
     1141 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactactgcg
     1201 ctaattcggt aactatcaac ttgagtccaa cccgaacaag cacgccaaag cgccattggc
     1261 agtagccatc ggtcgctcta cgggggaaag aaaatcttga ttgctgtaac agtaagcagt
     1321 tacacttgat aaacaatatt ggttactgtt cccctttaga gcagtcaccg aaggccgaaa
     1381 atgctggtaa cattttcggc ctgaaaagac cggcttatgc cggtcttttt ttatgcttta
     1441 ataatttttg aaaccatatc aacaactgta gttactgtct cgccggaaac gctatcaaca
     1501 gtacgtgccg ctctggcctt ccagtctaaa gacttcactt gatcagccaa gataacccct
     1561 gttgttttag tagtcccatc aacaggaact tcaaaagggt acccttttat ctgattagtt
     1621 atggggcaaa caacagcaaa cccagtgact tggttaaaca aatcatcaga aacgacaaga
     1681 gcaggccttt tccctgcctg ctcatgccct gattgagggt taaaatctaa aagaatgaca
     1741 tcgcctctgg ctggacaata tgtcgtcaca gcatctcctt accttgagag tcacatagat
     1801 actcgtgatg gcgattttct gcagtgcact gtccgagcag ttcttcaagc gtcgggccag
     1861 ctttagtaat tactaaatcc ctttcagtga cgcggaaatt aacaacatca ccgatactaa
     1921 gcccaagctg cttcatgaac ggctgcggga tacgcaaagc agcgccatta ccccatttac
     1981 caatagccgt agtcataata cctccaaagc agatggataa acaatgtgta aacaatattt
     2041 gtattgtata gtctttgagt tcagtttgaa gggtggcacc aatgccaccc taaaaagggg
     2101 taactactta aacaaataaa tacttatttg tttaagtagt taccaatggc aaaaaaaatt
     2161 tttttttaac taagtggcga taacaaattg atttacaaag aaaaaaaaat aaaccgcaac
     2221 caaataccag cgatcttagc aagcaggtgg ggatatagca tcctagagga agcagggtgg
     2281 acaactgttc ctaatttatt gcttgaaaaa cagatggttc tagaattgag taacaccgaa
     2341 atgctgttac tcattcatct aatctctttc attcactttg cagacacccc agcattccca
     2401 agcatttcta cactagcagg taggatcaac caacacccaa gaaccgtgca gcgaaccata
     2461 aacaaattaa tatctaaagg gttcttgaag agaaaaatcc gcgcaaaatc agtcaatgac
     2521 aaaggcttga gcaatcttta cagctttgaa cctttgatag aaaagctatt aatatcaatt
     2581 agtaacaacg aagaccagta tgaagaactt cagaaagaac aaggagaaag agacttgaaa
     2641 acagtagatc aattagttcg gattgctgca gcaggaggtg gtttacgttt aagtgcatcc
     2701 acaaaaacag tggatcagct cgtgcggatt gccgcagccg caggtaactc gataaccccc
     2761 aataaagcaa agttagttct gtttgatctt gagtccaaga ctgttgatga gctagttcga
     2821 attgctgcag caggaaaggg gtgtgtacta ttcgaattgg agtaacacac taaaaagatc
     2881 actataagtt taaacatcaa accagtctta atttaacata aagccctatt acgtgcaccg
     2941 ctaaaacggc ttcagggcgt ttctgacggg aaaccggcaa aacaggcaac aaaccacccg
     3001 aaaacctgcc agaaacgcgc aggggctgtt tttacggcat acatgagtat gcatgcaagt
     3061 gcatagcgcg ggcattcatg ccattcagcc agggtaagtc ctgtttccgg cgtttttctg
     3121 atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaggcgt tgccggcgca
     3181 gccggaaaac ccgttttcag cacgttcaag aatccagttc agcgacagcc ggcaatgacg
     3241 tgatggcaag tcaccggacg acttagcaca cgattcaccg gcgattcgac tcgtgacttg
     3301 actctagagt caccaaggtc aaaaaaacac caaatgaacg actcaactct agagtcaagt
     3361 cgtcataagt tactgata
//
