LOCUS       CP052210                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain E17KP0085 plasmid pE17KP0085-3,
            complete sequence.
ACCESSION   CP052210
VERSION     CP052210.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609950
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:27:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,614
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,375
            CDSs (with protein)               :: 5,375
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 58 of 116
            Pseudo Genes (incomplete)         :: 54 of 116
            Pseudo Genes (internal stop)      :: 19 of 116
            Pseudo Genes (multiple problems)  :: 12 of 116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="E17KP0085"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE17KP0085-3"
                     /geo_loc_name="South Korea: Jeonnam"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            250..669
                     /gene="exc2"
                     /locus_tag="HJX62_27975"
     CDS             250..669
                     /gene="exc2"
                     /locus_tag="HJX62_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJO70212.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            975..1238
                     /locus_tag="HJX62_27980"
     CDS             975..1238
                     /locus_tag="HJX62_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJO70213.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            1228..1527
                     /locus_tag="HJX62_27985"
     CDS             1228..1527
                     /locus_tag="HJX62_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJO70214.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1589..1966)
                     /locus_tag="HJX62_27990"
     CDS             complement(1589..1966)
                     /locus_tag="HJX62_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJO70215.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            2035..2214
                     /locus_tag="HJX62_27995"
     CDS             2035..2214
                     /locus_tag="HJX62_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO70216.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2242..2772)
                     /locus_tag="HJX62_28000"
     CDS             complement(2242..2772)
                     /locus_tag="HJX62_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO70217.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2779..3510)
                     /locus_tag="HJX62_28005"
     CDS             complement(2779..3510)
                     /locus_tag="HJX62_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJO70218.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(3510..5474)
                     /locus_tag="HJX62_28010"
     CDS             complement(3510..5474)
                     /locus_tag="HJX62_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJO70219.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6955..7104)
                     /locus_tag="HJX62_28015"
     CDS             complement(6955..7104)
                     /locus_tag="HJX62_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJO70220.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7189..7446)
                     /locus_tag="HJX62_28020"
     CDS             complement(7189..7446)
                     /locus_tag="HJX62_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO70221.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(7456..9141)
                     /locus_tag="HJX62_28025"
     CDS             complement(7456..9141)
                     /locus_tag="HJX62_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO70222.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     BGC             2456..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6274..6852
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2783..3510)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ccactcacac catgcgcaat cccctccaga tgcaacaaaa accagcacaa acggcgccgt
       61 tctccggcct cagtatgttt tactgaaatc attatatgag ataccagaaa gccccctccg
      121 ggacaggaaa agaaacgggg ggtctggttc gggttgttcg gtcactgaac acggtcagaa
      181 actgatgata agatagcctg cggatatata actcaatgag aaaaaagaat taaaggtaag
      241 gagacgctca tgagacgttc cggcagcaaa ctgtgtctta tcgccatgat aacagcgaca
      301 gcaactctca ctgcctgtgc tccaaagggc agcgtggaac agcatacccg gcattacgta
      361 tatgcttcag atgacggttt tgatcccaat ttttccaccc agaaagctga cacaacacga
      421 atgatggtgc cttttttccg gcagttctgg gatatgggcg caaaggataa agcgaccgga
      481 aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc acagtcagga atttttaaac
      541 tcactccaag gcacaacaca atttgcgggt accgattacc gcagcaaaga cctcacaccg
      601 aaaaaatcaa ggctactggc tgacaccatt tcagctgttt atctcgatgg ctatgaaggc
      661 agacagtaag tggatttacc ataacgcgtc attacgcgca ccgctcagac gggctcaggg
      721 cgtttccggc ggctacggga taaaacaggc aacaaccccc ccggaaaact gccggaaacg
      781 cgcccgataa attttatcct aatgcatgcc tatgcacgca actgcatggc atatgttttg
      841 acgtaaaatg gatgccgtaa gccctttttt accgactttc tgcttcatca gcctgactga
      901 cacgaccata ttctttcaga ccgccccctg ctaaacttcc ttcatattgc cgcattaacc
      961 cgggaggctc cgcaatgagc aacactacac actacgaaaa cgccaacttt cttcgggaac
     1021 tggctgaaag cctgccccgc atactgccgg aaggtggccc ggacaaagct gcgctgttgc
     1081 agcgactggc taacgaagaa ctggcccagg cagagtatga agatcaggtt cgcgccaaag
     1141 tgaccgccgc ccgtgctgac acgcgtccgg gtatgaccac ggaacaactc cgccagcgcc
     1201 tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg tggaatggaa gcagggagcc
     1261 atcgaggatg taacgacgct gtttgactat atcgcggaaa attcatccct gtgggatgcg
     1321 cagaacgtta ccgaccgggt tctcgctgcg gcggacaaac tggccgactt cccgcgactc
     1381 tatgaagtgg actcacgcta tggcgaggac gttcgccgga tcagtctgat gggacagcat
     1441 gtcctctatg atgttgatga ccagaccaga aaagtccggg ttctggccgt ggtcggccag
     1501 cgccagaatc cccatattgt tctctaataa ccggtgacgg ggacgcaatt cattgcgtgg
     1561 ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc ccacggccac caccggcgcg
     1621 gtgttccggc cggggtctct gctgtctccg gtttgtgttc cagcagtctc atggactgca
     1681 tttcctgccg tagcccggcc acctcctccc tgagtgcttt tatctccgcg atcaacgcgt
     1741 cggaggccat tttttcagtc tcacccgaca gtctcaactc gtcccgctcg tgagtctcat
     1801 cctgtcttaa ctcaccgtac acccggataa gctcgctggt atccaccacc cggccgccat
     1861 cagcttccgt acggtatgat acgcggcctt tcgccatatc ccgatacagg gaacttcggg
     1921 ctttacccgt cagttccgtc gcctgtctca ccgtcaccca cgccattgtc tcaccccgct
     1981 atttttgaga cagtatacct gacaggcgca caccatgccc ggaaggaaag aatcatgaca
     2041 aaacaggaaa aaacagccct caatatggct aaatttatta aggagcagtc gctgctattg
     2101 ctcgataaac tgaatgagct ggatatggat accccggccg acatgtgcga gcggctacac
     2161 gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcggggagga ataacagcac
     2221 cggcatggcc ggtgctggca tttatttttt gccgaacggc caccaccggc gggcaggttt
     2281 gtcctccggc agttcaggta ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag
     2341 ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcactca ccacgtcaat
     2401 catcgcgtta atctgctccg gtgagcactt cccggcatca accacctgca caccgttctc
     2461 gatggcgaac cgctccacct tgcgatagag gtaatcacac agcttcattc ctgtgggttt
     2521 gttccgttcc cattgcggat ttttaccagt gaaaaagtac gtggcttttt cgtcgctgtt
     2581 gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg cccggtcgac
     2641 aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatatctt ccagatgctc
     2701 gctggtcggc atgatgcagc gctcgccgtt ccggtcctcc gcgtcttcaa taaataccat
     2761 gtacagcgcc ataatccctc actccgttgt cataaattta aagcacgcaa acaaccgttc
     2821 acgatccagc tctgccgttt tgcgggcctc gccggtctgt gggttcacga tttcatccac
     2881 cgacaggatt gcccgctgca ccctgacctg cgtcgcccgg tccgggcaga tgtaccagat
     2941 ttcatcgatg ccgttagctt tgcggttgaa cagatacccg gacaccactt cctgataccg
     3001 gcgacggctc ttgacggtgc gctccacctc aaacgccacc gttttaccgt ccagctccgc
     3061 cagggcgtcc ggcactttca tgcctttaag actcagacgg tgcagaatgc gccagccggt
     3121 ggcccctctg gcttccatat caaggcgcag ggattgcacg gccagccggt gcggcagatg
     3181 tgctgccgac acccttgacg gctgaaacgg aataacatcc gccagcggtt cgtcctcgtc
     3241 gaacgagaac gccacgccat gcggcgtcag cccccacacg ctgacctgac ggccgtccac
     3301 cggatatttg gtccggacca gcagcccttt cgccaccagg ctgtcgagga agcggtagac
     3361 cggctgcacc gccgcaatcc ccagccgccg cgcaaccagc tccgccgtgg aacacagctc
     3421 ttcccgcaac cagcgcaggg tcagccgggt tttctccgcg ttccgcgcct gtcgtttcgc
     3481 gtggctgacg ttgtacagct ccagcgccat cagtaaatcc ccgcaaaatc atcatccgct
     3541 ttcggcccgc cgccgtcggc cgggggccgg aactccgcca ggggatcggt tttcgccagc
     3601 accgggaaac gctgcaacgg gatttcccgg cgctcaaccc gcagcggact gctgaacgcc
     3661 atccgggcca cacccagccc ggtcatgacc gcgaacccgt ccggcaggtg ttgcagggtg
     3721 ttcacgtcaa acagcggccg cgcctccttc tggatgaagc tgtccccgct gatatgctcc
     3781 acgttccccg cctcccggct ggcacggcgg cgctccacgt ccaccctgat ttccccggtc
     3841 tgccccgccg cccactgcgc ggactccagg ctggccgccc ggtaaaacca gcggatcggc
     3901 gtgttatcca gtaccgtggt tttcacaaat tcagcgggca gatcctgccc gcactgcccg
     3961 aaatcgccca gcgactggtg cgcgagcaac agattgcagt cccggctgcg cagcgtcccc
     4021 agtgcgttca gtacatactt cgacagcaga tatttaattt catcgagcat cacgctgacg
     4081 tgtggccact cccggaactc gtcccgcgca atgatgattt gcgcgcagcg ggcaaacagc
     4141 attttttgca cacggatcac cgcctcgtcg tccatcgagc cgataacgta caggcagccg
     4201 ccgccgttga taatccccgc cacatcgatg ccactgtccg tctgcaggac gctcaggtca
     4261 gcgacgtttt ccagcctagt gatcaacccc ttcacgtcct ttttcagggc ctccggcagc
     4321 gcgtacgccg ccgccagcac ctgctgaatg tttgccccct gcggaaactg ctccgcaatc
     4381 agcttcgccg ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc ggtctcgccc
     4441 aggttaagcc cggcaatcag caggtttttc agcgcgtact ggtcgagatc gcggaacaga
     4501 ttcagctgcg ccggtttccc cggccgcaga tccagcaggg tgaagtccgg acagtgcgcc
     4561 cggaagacgt gcggcgccca tgcgtcccct ttggggtcga agtacaccac cgcgtccccg
     4621 aaatcgcgta cgcagcgaat gagcgcgttg gtggccatca cgctcttgcc gctcccgggc
     4681 aggccgagga tctgaatgtt ggtcttgcgc caggtatcac gcggaaccag caccggcttg
     4741 ccctgctcat ccttacccag aaacaccgcc tgctcctgcc gggcctgctc cgaggaaaaa
     4801 tcggccagcg cattaaccgg taccggctcc ccgaaatgca ggtggcgcac gtcggtcatc
     4861 tctcctgtcc gctggctgct gcgccggctc tcctgattca gtttcgcgtt caccgcctcg
     4921 tcagcagccg caaaatgacc gtcaagcagc aggaacgcca gcagccgcgc caacacaatc
     4981 ccgacagcca gcccgcccgc gcaccaggca atcccgaaca ggttagccgg ctgcagcagg
     5041 taataatctg cgtttgcgta gaggtccgga cgcaggacgg tttctcccca gctctcgata
     5101 aacacggcga caaccagccc ccccagaagg gccagaccgg ccgcgccggt cagccagtac
     5161 gcgtagttcc agacatgtcg acgtgccctg accatccgcc cgggcgaatc cagcgaggcc
     5221 agcaccgaaa atggcgctgg ccgtttatta tcgcgcttat agcgatcggc atactcccgt
     5281 cgctccttat gactcagccc cacaaaacgc atccgtccgg cccaaagttc agccagaccg
     5341 aaaaacaggc cgataactag gaaagccccg gccagaccgt aatactcctg gtacagccag
     5401 ctgacccacg gataaatctg ccgggccagc tccggaatgt gccacttcca ggcaagcgaa
     5461 tccgtattaa acatgttctc tctccttcag acgatggttt gccggcgtga tgtgcagccc
     5521 ccgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg tccgtggtga
     5581 ttgcgttcag ctttcaccac ggaggccagc ctctttccgc cccatgcgga aaaggcgcag
     5641 ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg
     5701 gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc
     5761 aaaatcagga gcagctttgc tgcttcgcca ggtgcgtcca gcaccgtgga tttttgtggg
     5821 tggtcaggcg tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg
     5881 gacggagcca gaaagagggg gagagaccgt gcgtcggtgg cgtccagcca gcgtaagcag
     5941 gggatatccc cctgaggtca gccacgccct cgcggggatg acctgtattt ctccgtaagg
     6001 agaaaaaagc aacagagata ggttaacgta aaatgttcag cgaaaaaccg atatgattca
     6061 cctatcctgg taatttatta tcagggaata taaagactta atcaggggac aatatgccac
     6121 atcagcgcgt ccggtgtccg tcttgtaggt gccggggaaa ttgcgcctcc tcgctcactc
     6181 ggtcgctacg ctccggccgt gagactgcgg cgggcgctgc ggacacatgc aaagttaccc
     6241 acagattccg tgaataagca ggggactaac atgtgaggca aaacggcaag gccacgcagg
     6301 tggcgttttt ccataggctc cgccctcctg actgagttca cataaacaga cgctttttcg
     6361 gtgcatctgt gggagccggt ggctcaacca tgaatcagtc aggagtggcg aaacccgaca
     6421 ggacttaaag atcatcaccg tttccgatga atcgctccct cttgcgctct cctgttccaa
     6481 ccctgccgtt tcccggatac ctgttccgcc tttctccctt gcgggaagtg tggcgctttc
     6541 tcatagctca cactctggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt
     6601 aagcaagaac cccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag
     6661 tccaacccgg aaaagcacgc aaaacgccac tggcagcagc cattggtaac tgggagttcg
     6721 cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta
     6781 cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag
     6841 ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag
     6901 ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg
     6961 aaccgcgatc cccgtcagtt cagaggagga cgatggtgcc accgttccac cctgaacatc
     7021 ccggatatag tttgcctgac atgcgaccag aagaaagcct gaaaaaatca atataaaaag
     7081 aaaaacaacc tttacctttt tcatatacct tccctgtatt taaaaacccc ggtattcatc
     7141 cctgaacacc ggaagtctgt atttcaaatc atccttgata acttttgctt accagttacc
     7201 atcccggtaa acaaaggata caaagtaatc aaatttacta atatcgatta catttttaaa
     7261 gtgtggctgt aatatcgaaa cccatgattt ttcaacatca aaccaaccat tattaatatt
     7321 atcctttaaa ggcatcccca gactttcaat gacagaacca tcatcaccga agtcttttga
     7381 gtattcacca cctttaaact cttcggtttt cttatcaaac caatgaatat gtaatttaag
     7441 ccccatattt acctcttaaa gatatttttt aatgtttcgt tttggatccg gccctttaac
     7501 ctgcttaccc gtttttggat cgaatgcccc gaggtgttcg ccatcactgg cgcggtaccc
     7561 ttcaagctca ccgtgctggg aatcccattc ataaatttta cgctttttat caccatacca
     7621 tcgggcccgc ttaccaccac caccttgttt aggggtttta ggtttaccct ctttcaactc
     7681 tcccaacccc ttaatatctt cagtcttggg atcaggaaaa taatcatggc cgtaatcttt
     7741 agttccctta cgaggttttt tcttttcctc atttaattta ttttctgcat ccttcgcttt
     7801 tttctccttt tgtttccggc tctccatcgc cccggaaagc gcagccttat ccctggataa
     7861 ggattcattc atttcgttta aagatgtctg ggcagctttt aatttctcat tctgattctg
     7921 aatatcctta tcaagatagc gaacctgatc ctgccacccg gagccatatt catgaggcgg
     7981 gttataagtc acaaaatcat ctttatttgc ctctgcctct tttacctttt tatcggcttc
     8041 ctgaacagca gcccttgcac cggggatcgc cttctctgtc gatgcaatgc gactgtttaa
     8101 ggtcgcaata tttttatctt cagcatccag ctcagctttc gctttatcat actctctttt
     8161 cagcccctct tccgggtgag cagcgtccca tgcctgctgg cgacgctttt cttcctccag
     8221 acgctgtttt acctgtgccg gagtaagaac atccgtcacc gacacataga ccggtttctg
     8281 tccgctctct ttcgggaaac gaataacggc ctcacgggag ttaccaccgg ccgtaaaacc
     8341 agccggacgt gaatcacctt tttcagcaat aatgcctttt ggcggggctt tcgcggccgg
     8401 aacaccttta ggtacgctca cctgcagaga cgggagaccc ggaatcgaca cactgaatac
     8461 ccccggacgt tttgtcggtt tcgcatccac cacaggaaca ctcattggcc ggccggtgac
     8521 aaccgcaata tgctgccgct cgtccttcac aatatccgcc acacgtttag tgacgctgac
     8581 cgtcgcctgg tccagcggca gggagctgac cggcgtctcc gttaccgtgt ctgccggcaa
     8641 tgacgtcacg atttttgaca tcattttcgg atcatctttt gctatctcag aaggcagcac
     8701 tccgtaaatc gcaatccccc acagaccaaa cttaaacgga cctttcaggg cagccagcac
     8761 atcagcaaca gcggccgaca acgcatcgcc ggaaacggat aaagccagtc cttcagcccc
     8821 cggagtggcc agggcaggca gaccgaaggc catcgcggtg gccgaagact gtccgccacc
     8881 agagctcccg ctatgattgc cgctgttacc gccattacca ccgggtttac tcccgccgtt
     8941 accaaactcc cccgggttat agttatgaat atccccgtta ggtgtatgcg tcgtacccca
     9001 gcccggcccg ctgtttggat ccgtaccacc acctgatgaa gggccacctt tacctgatgt
     9061 accgttcaca ttcccactgg cctgaccacc attgtgtccc agacctgaat tacccggacc
     9121 tcgtccgtct ccgccactca tatacttttc ctctttgact tttaaaacaa taaactaaaa
     9181 taaaatactg tacatataac cactggtttt atatacagta taaaaattac gctgccttgt
     9241 ttttcatgtc aagtctgtaa atttgcgttt ttgtgaaaat gatcaaaaaa acag
//
