LOCUS       CP052206                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0002 plasmid pF16KP0002-3,
            complete sequence.
ACCESSION   CP052206
VERSION     CP052206.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609952
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:33:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,599
            CDSs (total)                      :: 5,476
            Genes (coding)                    :: 5,321
            CDSs (with protein)               :: 5,321
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 74 of 155
            Pseudo Genes (incomplete)         :: 75 of 155
            Pseudo Genes (internal stop)      :: 34 of 155
            Pseudo Genes (multiple problems)  :: 27 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0002"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0002-3"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(9232..9294,1..669))
                     /locus_tag="HJX64_27945"
     CDS             complement(join(9232..9294,1..669))
                     /locus_tag="HJX64_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ51706.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(669..2633)
                     /locus_tag="HJX64_27950"
     CDS             complement(669..2633)
                     /locus_tag="HJX64_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ51696.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4114..4263)
                     /locus_tag="HJX64_27955"
     CDS             complement(4114..4263)
                     /locus_tag="HJX64_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ51697.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4348..4605)
                     /locus_tag="HJX64_27960"
     CDS             complement(4348..4605)
                     /locus_tag="HJX64_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ51698.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(4615..6300)
                     /locus_tag="HJX64_27965"
     CDS             complement(4615..6300)
                     /locus_tag="HJX64_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ51699.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            6703..7122
                     /gene="exc2"
                     /locus_tag="HJX64_27970"
     CDS             6703..7122
                     /gene="exc2"
                     /locus_tag="HJX64_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ51700.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            7428..7691
                     /locus_tag="HJX64_27975"
     CDS             7428..7691
                     /locus_tag="HJX64_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ51701.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            7681..7980
                     /locus_tag="HJX64_27980"
     CDS             7681..7980
                     /locus_tag="HJX64_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ51702.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8042..8419)
                     /locus_tag="HJX64_27985"
     CDS             complement(8042..8419)
                     /locus_tag="HJX64_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ51703.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            8488..8667
                     /locus_tag="HJX64_27990"
     CDS             8488..8667
                     /locus_tag="HJX64_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ51704.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8695..9225)
                     /locus_tag="HJX64_27995"
     CDS             complement(8695..9225)
                     /locus_tag="HJX64_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ51705.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3433..4011
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..669)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="92.0"
                     /evalue="5.4e-136"
ORIGIN
        1 gcgggcctcg ccggtctgtg ggttcacgat ttcatccacc gacaggattg cccgctgcac
       61 cctgacctgc gtcgcccggt ccgggcagat gtaccagatt tcatcgatgc cgttagcttt
      121 gcggttgaac agatacccgg acaccacttc ctgataccgg cgacggctct tgacggtgcg
      181 ctccacctca aacgccaccg ttttaccgtc cagctccgcc agggcgtccg gcactttcat
      241 gcctttaaga ctcagacggt gcagaatgcg ccagccggtg gcccctctgg cttccatatc
      301 aaggcgcagg gattgcacgg ccagccggtg cggcagatgt gctgccgaca cccttgacgg
      361 ctgaaacgga ataacatccg ccagcggttc gtcctcgtcg aacgagaacg ccacgccatg
      421 cggcgtcagc ccccacacgc tgacctgacg gccgtccacc ggatatttgg tccggaccag
      481 cagccctttc gccaccaggc tgtcgaggaa gcggtagacc ggctgcaccg ccgcaatccc
      541 cagccgccgc gcaaccagct ccgccgtgga acacagctct tcccgcaacc agcgcagggt
      601 cagccgggtt ttctccgcgt tccgcgcctg tcgtttcgcg tggctgacgt tgtacagctc
      661 cagcgccatc agtaaatccc cgcaaaatca tcatccgctt tcggcccgcc gccgtcggcc
      721 gggggccgga actccgccag gggatcggtt ttcgccagca ccgggaaacg ctgcaacggg
      781 atttcccggc gctcaacccg cagcggactg ctgaacgcca tccgggccac acccagcccg
      841 gtcatgaccg cgaacccgtc cggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc
      901 gcctccttct ggatgaagct gtccccgctg atatgctcca cgttccccgc ctcccggctg
      961 gcacggcggc gctccacgtc caccctgatt tccccggtct gccccgccgc ccactgcgcg
     1021 gactccaggc tggccgcccg gtaaaaccag cggatcggcg tgttatccag taccgtggtt
     1081 ttcacaaatt cagcgggcag atcctgcccg cactgcccga aatcgcccag cgactggtgc
     1141 gcgagcaaca gattgcagtc ccggctgcgc agcgtcccca gtgcgttcag tacatacttc
     1201 gacagcagat atttaatttc atcgagcatc acgctgacgt gtggccactc ccggaactcg
     1261 tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca ttttttgcac acggatcacc
     1321 gcctcgtcgt ccatcgagcc gataacgtac aggcagccgc cgccgttgat aatccccgcc
     1381 acatcgatgc cactgtccgt ctgcaggacg ctcaggtcag cgacgttttc cagcctagtg
     1441 atcaacccct tcacgtcctt tttcagggcc tccggcagcg cgtacgccgc cgccagcacc
     1501 tgctgaatgt ttgccccctg cggaaactgc tccgcaatca gcttcgccgc tttctgctcg
     1561 ctgatgcggt agtggtccgc cacgtcgccg gtctcgccca ggttaagccc ggcaatcagc
     1621 aggtttttca gcgcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc
     1681 ggccgcagat ccagcagggt gaagtccgga cagtgcgccc ggaagacgtg cggcgcccat
     1741 gcgtcccctt tggggtcgaa gtacaccacc gcgtccccga aatcgcgtac gcagcgaatg
     1801 agcgcgttgg tggccatcac gctcttgccg ctcccgggca ggccgaggat ctgaatgttg
     1861 gtcttgcgcc aggtatcacg cggaaccagc accggcttgc cctgctcatc cttacccaga
     1921 aacaccgcct gctcctgccg ggcctgctcc gaggaaaaat cggccagcgc attaaccggt
     1981 accggctccc cgaaatgcag gtggcgcacg tcggtcatct ctcctgtccg ctggctgctg
     2041 cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcagccgc aaaatgaccg
     2101 tcaagcagca ggaacgccag cagccgcgcc aacacaatcc cgacagccag cccgcccgcg
     2161 caccaggcaa tcccgaacag gttagccggc tgcagcaggt aataatctgc gtttgcgtag
     2221 aggtccggac gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc
     2281 cccagaaggg ccagaccggc cgcgccggtc agccagtacg cgtagttcca gacatgtcga
     2341 cgtgccctga ccatccgccc gggcgaatcc agcgaggcca gcaccgaaaa tggcgctggc
     2401 cgtttattat cgcgcttata gcgatcggca tactcccgtc gctccttatg actcagcccc
     2461 acaaaacgca tccgtccggc ccaaagttca gccagaccga aaaacaggcc gataactagg
     2521 aaagccccgg ccagaccgta atactcctgg tacagccagc tgacccacgg ataaatctgc
     2581 cgggccagct ccggaatgtg ccacttccag gcaagcgaat ccgtattaaa catgttctct
     2641 ctccttcaga cgatggtttg ccggcgtgat gtgcagcccc cgctgcacaa gccgcggaca
     2701 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccacg
     2761 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
     2821 gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc
     2881 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
     2941 gcttcgccag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg
     3001 ggggccgggt gaatttctgg cgtggccggc gtccagccgg acggagccag aaagaggggg
     3061 agagaccgtg cgtcggtggc gtccagccag cgtaagcagg ggatatcccc ctgaggtcag
     3121 ccacgccctc gcggggatga cctgtatttc tccgtaagga gaaaaaagca acagagatag
     3181 gttaacgtaa aatgttcagc gaaaaaccga tatgattcac ctatcctggt aatttattat
     3241 cagggaatat aaagacttaa tcaggggaca atatgccaca tcagcgcgtc cggtgtccgt
     3301 cttgtaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccggccgtg
     3361 agactgcggc gggcgctgcg gacacatgca aagttaccca cagattccgt gaataagcag
     3421 gggactaaca tgtgaggcaa aacggcaagg ccacgcaggt ggcgtttttc cataggctcc
     3481 gccctcctga ctgagttcac ataaacagac gctttttcgg tgcatctgtg ggagccggtg
     3541 gctcaaccat gaatcagtca ggagtggcga aacccgacag gacttaaaga tcatcaccgt
     3601 ttccgatgaa tcgctccctc ttgcgctctc ctgttccaac cctgccgttt cccggatacc
     3661 tgttccgcct ttctcccttg cgggaagtgt ggcgctttct catagctcac actctggtat
     3721 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaacc ccccgttcag
     3781 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacgca
     3841 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     3901 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     3961 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     4021 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     4081 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
     4141 agaggaggac gatggtgcca ccgttccacc ctgaacatcc cggatatagt ttgcctgaca
     4201 tgcgaccaga agaaagcctg aaaaaatcaa tataaaaaga aaaacaacct ttaccttttt
     4261 catatacctt ccctgtattt aaaaaccccg gtattcatcc ctgaacaccg gaagtctgta
     4321 tttcaaatca tccttgataa cttttgctta ccagttacca tcccggtaaa caaaggatac
     4381 aaagtaatca aatttactaa tatcgattac atttttaaag tgtggctgta atatcgaaac
     4441 ccatgatttt tcaacatcaa accaaccatt attaatatta tcctttaaag gcatccccag
     4501 actttcaatg acagaaccat catcaccgaa gtcttttgag tattcaccac ctttaaactc
     4561 ttcggttttc ttatcaaacc aatgaatatg taatttaagc cccatattta cctcttaaag
     4621 atatttttta atgtttcgtt ttggatccgg ccctttaacc tgcttacccg tttttggatc
     4681 gaatgccccg aggtgttcgc catcactggc gcggtaccct tcaagctcac cgtgctggga
     4741 atcccattca taaattttac gctttttatc accataccat cgggcccgct taccaccacc
     4801 accttgttta ggggttttag gtttaccctc tttcaactct cccaacccct taatatcttc
     4861 agtcttggga tcaggaaaat aatcatggcc gtaatcttta gttcccttac gaggtttttt
     4921 cttttcctca tttaatttat tttctgcatc cttcgctttt ttctcctttt gtttccggct
     4981 ctccatcgcc ccggaaagcg cagccttatc cctggataag gattcattca tttcgtttaa
     5041 agatgtctgg gcagctttta atttctcatt ctgattctga atatccttat caagatagcg
     5101 aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc
     5161 tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc
     5221 ggggatcgcc ttctctgtcg atgcaatgcg actgtttaag gtcgcaatat ttttatcttc
     5281 agcatccagc tcagctttcg ctttatcata ctctcttttc agcccctctt ccgggtgagc
     5341 agcgtcccat gcctgctggc gacgcttttc ttcctccaga cgctgtttta cctgtgccgg
     5401 agtaagaaca tccgtcaccg acacatagac cggtttctgt ccgctctctt tcgggaaacg
     5461 aataacggcc tcacgggagt taccaccggc cgtaaaacca gccggacgtg aatcaccttt
     5521 ttcagcaata atgccttttg gcggggcttt cgcggccgga acacctttag gtacgctcac
     5581 ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt
     5641 cgcatccacc acaggaacac tcattggccg gccggtgaca accgcaatat gctgccgctc
     5701 gtccttcaca atatccgcca cacgtttagt gacgctgacc gtcgcctggt ccagcggcag
     5761 ggagctgacc ggcgtctccg ttaccgtgtc tgccggcaat gacgtcacga tttttgacat
     5821 cattttcgga tcatcttttg ctatctcaga aggcagcact ccgtaaatcg caatccccca
     5881 cagaccaaac ttaaacggac ctttcagggc agccagcaca tcagcaacag cggccgacaa
     5941 cgcatcgccg gaaacggata aagccagtcc ttcagccccc ggagtggcca gggcaggcag
     6001 accgaaggcc atcgcggtgg ccgaagactg tccgccacca gagctcccgc tatgattgcc
     6061 gctgttaccg ccattaccac cgggtttact cccgccgtta ccaaactccc ccgggttata
     6121 gttatgaata tccccgttag gtgtatgcgt cgtaccccag cccggcccgc tgtttggatc
     6181 cgtaccacca cctgatgaag ggccaccttt acctgatgta ccgttcacat tcccactggc
     6241 ctgaccacca ttgtgtccca gacctgaatt acccggacct cgtccgtctc cgccactcat
     6301 atacttttcc tctttgactt ttaaaacaat aaactaaaat aaaatactgt acatataacc
     6361 actggtttta tatacagtat aaaaattacg ctgccttgtt tttcatgtca agtctgtaaa
     6421 tttgcgtttt tgtgaaaatg atcaaaaaaa cagccactca caccatgcgc aatcccctcc
     6481 agatgcaaca aaaaccagca caaacggcgc cgttctccgg cctcagtatg ttttactgaa
     6541 atcattatat gagataccag aaagccccct ccgggacagg aaaagaaacg gggggtctgg
     6601 ttcgggttgt tcggtcactg aacacggtca gaaactgatg ataagatagc ctgcggatat
     6661 ataactcaat gagaaaaaag aattaaaggt aaggagacgc tcatgagacg ttccggcagc
     6721 aaactgtgtc ttatcgccat gataacagcg acagcaactc tcactgcctg tgctccaaag
     6781 ggcagcgtgg aacagcatac ccggcattac gtatatgctt cagatgacgg ttttgatccc
     6841 aatttttcca cccagaaagc tgacacaaca cgaatgatgg tgcctttttt ccggcagttc
     6901 tgggatatgg gcgcaaagga taaagcgacc ggaaaatcac ggagtgatgt gcagcaacgc
     6961 attcagcagt ttcacagtca ggaattttta aactcactcc aaggcacaac acaatttgcg
     7021 ggtaccgatt accgcagcaa agacctcaca ccgaaaaaat caaggctact ggctgacacc
     7081 atttcagctg tttatctcga tggctatgaa ggcagacagt aagtggattt accataacgc
     7141 gtcattacgc gcaccgctca gacgggctca gggcgtttcc ggcggctacg ggataaaaca
     7201 ggcaacaacc cccccggaaa actgccggaa acgcgcccga taaattttat cctaatgcat
     7261 gcctatgcac gcaactgcat ggcatatgtt ttgacgtaaa atggatgccg taagcccttt
     7321 tttaccgact ttctgcttca tcagcctgac tgacacgacc atattctttc agaccgcccc
     7381 ctgctaaact tccttcatat tgccgcatta acccgggagg ctccgcaatg agcaacacta
     7441 cacactacga aaacgccaac tttcttcggg aactggctga aagcctgccc cgcatactgc
     7501 cggaaggtgg cccggacaaa gctgcgctgt tgcagcgact ggctaacgaa gaactggccc
     7561 aggcagagta tgaagatcag gttcgcgcca aagtgaccgc cgcccgtgct gacacgcgtc
     7621 cgggtatgac cacggaacaa ctccgccagc gcctgcatgg ccgttatcag gaactgcgcg
     7681 atgccgtatg acgtggaatg gaagcaggga gccatcgagg atgtaacgac gctgtttgac
     7741 tatatcgcgg aaaattcatc cctgtgggat gcgcagaacg ttaccgaccg ggttctcgct
     7801 gcggcggaca aactggccga cttcccgcga ctctatgaag tggactcacg ctatggcgag
     7861 gacgttcgcc ggatcagtct gatgggacag catgtcctct atgatgttga tgaccagacc
     7921 agaaaagtcc gggttctggc cgtggtcggc cagcgccaga atccccatat tgttctctaa
     7981 taaccggtga cggggacgca attcattgcg tggccgtcgc cggttggaca agcacagcgc
     8041 gtcagcgttt tccccacggc caccaccggc gcggtgttcc ggccggggtc tctgctgtct
     8101 ccggtttgtg ttccagcagt ctcatggact gcatttcctg ccgtagcccg gccacctcct
     8161 ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cattttttca gtctcacccg
     8221 acagtctcaa ctcgtcccgc tcgtgagtct catcctgtct taactcaccg tacacccgga
     8281 taagctcgct ggtatccacc acccggccgc catcagcttc cgtacggtat gatacgcggc
     8341 ctttcgccat atcccgatac agggaacttc gggctttacc cgtcagttcc gtcgcctgtc
     8401 tcaccgtcac ccacgccatt gtctcacccc gctatttttg agacagtata cctgacaggc
     8461 gcacaccatg cccggaagga aagaatcatg acaaaacagg aaaaaacagc cctcaatatg
     8521 gctaaattta ttaaggagca gtcgctgcta ttgctcgata aactgaatga gctggatatg
     8581 gataccccgg ccgacatgtg cgagcggcta cacgaggatg cggaaacact ctggcaggtg
     8641 atgcgggata agctcgggga ggaataacag caccggcatg gccggtgctg gcatttattt
     8701 tttgccgaac ggccaccacc ggcgggcagg tttgtcctcc ggcagttcag gtaccggcac
     8761 gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc
     8821 gctttccccg tgccgctcac tcaccacgtc aatcatcgcg ttaatctgct ccggtgagca
     8881 cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca ccttgcgata
     8941 gaggtaatca cacagcttca ttcctgtggg tttgttccgt tcccattgcg gatttttacc
     9001 agtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
     9061 tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg
     9121 ccaggcgagc accaccatat cttccagatg ctcgctggtc ggcatgatgc agcgctcgcc
     9181 gttccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc ctcactccgt
     9241 tgtcataaat ttaaagcacg caaacaaccg ttcacgatcc agctctgccg tttt
//
