LOCUS       CP052196                2628 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0014 plasmid pF16KP0014-4,
            complete sequence.
ACCESSION   CP052196
VERSION     CP052196.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609954
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:00:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,564
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 52 of 126
            Pseudo Genes (incomplete)         :: 68 of 126
            Pseudo Genes (internal stop)      :: 26 of 126
            Pseudo Genes (multiple problems)  :: 17 of 126
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0014"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0014-4"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(348..770)
                     /locus_tag="HJX66_28405"
     CDS             complement(348..770)
                     /locus_tag="HJX66_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJO59557.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHAKDIKVID
                     TSELIRVFGSLDGVIDTVKYDVKIDAGSTGINSVGLHDLQHRIALLEAENNGLKGAVRA
                     RDEHIDSLRQAMQLLEHKHEPSSSPHSTWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(770..961)
                     /locus_tag="HJX66_28410"
     CDS             complement(770..961)
                     /locus_tag="HJX66_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO59558.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDFDVEADLCEKLHDDA
                     EHLFRTLTSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1734..2192)
                     /locus_tag="HJX66_28415"
     CDS             complement(1734..2192)
                     /locus_tag="HJX66_28415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO59559.1"
                     /translation="MKGKTSHFFLFFVFCFSCFFSSVLSARELTKEEVEAVEIGVGQQL
                     KDPYSAKYTHGSYMFEGRSGGVYCGKVNAKNSYGAYIGDRLFAVAFIDTKSDGLLAVPV
                     GSMDSQEAISSLCASSGYDLRVNKIFKDDVNKERARKGFSKLDDAYFY"
                     /name="hypothetical protein"
     Typing          1007..1553
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.909"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gactctagag tcaccatggt caaaaagcca ccaacccggc aagtcgactc tagagtcaag
       61 tcatcataac gtactgatta tatggcgtga ttcacatatg aatcgtgggc aagtcatcgg
      121 cgattcgcca tagagtcgtc cacgactcgg attcacctta caaaaaataa aattgcgtaa
      181 tttaaaatag ataaccaatt gaaactaaag gtctatctgc aaaattttaa ttttgcgtag
      241 tgtgtgggta tttttagtgg tgaggagcgt tctgagatct ggcgattttt gggaaggttc
      301 aggaactccc cgggatggcc ggagaggaaa actcaagcat ggcaggatca ggattttttc
      361 cagaatttcc accacgtgga atggggtgat gaagaaggtt cgtgcttgtg ctccagaagc
      421 tgcatagcct gacgaaggga atcgatatgc tcatctcgag ccctaacagc tcctttaaga
      481 ccattgtttt cagcttcaag taaagcgatt ctatgttgta aatcatgtaa accaacgctg
      541 ttaatacctg tagatcctgc gtcaattttt acatcatact ttacagtatc gatgacacca
      601 tcaagagaac caaacactct aataagctct gaagtatcaa taactttaat atcctttgcg
      661 tgctttacaa cagaggtgta acttaacttc ccactgttaa tcatcttgta aattgtgggg
      721 cgactaacct taacaagccg tgccgcttca ctaatagaca ctctggccat cagttaccat
      781 cctgtaattc atccagtctc gaggtcagtg tacggaaaag atgctccgca tcatcgtgga
      841 gtttctcaca aaggtcggcc tcgacatcga aatcaagctc gttaagcttc tccagcagga
      901 gcagagactg attctggatg aatttagcca tgttcagcgc tgttttctgt tgttttgtca
      961 taagaggcct cagagcgtca gtgctcagtg tagggatgtt ctcttttccg cttcctcgct
     1021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtgcggactt acaaacaggg
     1081 cggaaatttc ctggtagatg ccaggaagga acgtaacagg gaaaccatga cgccgcggca
     1141 aagccgtttt tccataggct ccgcccccct gatgagcatc acagaatctg acactcaaat
     1201 cagtggtggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     1261 ctcatgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     1321 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctggg
     1381 ctatatgcac gaaccccccg tttagcccga ctgccacgcc ctgtaaggta actaatctct
     1441 tgagtccaac ccttacggac acgatacatc gccactggcg gcagccattg gtaacgggtg
     1501 tacaagatat tactgatgca gagttcttga agttaaagcc taactgcggc taaactagaa
     1561 ggacaaatta ggttgcagtg gtcttgttca ccctgttacc acggttcaga agagttacag
     1621 ctcaactgat ccatcgaaag agccacctta cagggtggct ttttcaattc aacgggtaaa
     1681 ttacgccaac aattaggatt cagtcaattt tttactactc agtaagctgc tagctaatag
     1741 aaataagcat catccagctt agaaaaaccc ttccgtgctc gctctttatt cacatcatct
     1801 ttaaatattt tattcactct aagatcataa ccagaagatg cacaaagaga tgaaatagcc
     1861 tcttggctat ccatactgcc aactggtacg gctagcagtc catctgattt agtatcaata
     1921 aaggctactg caaacaaacg gtctccaatg tatgcaccat aagaattttt cgcattaacc
     1981 ttcccacaat acacaccccc gctacgccct tcgaacatat aggagccatg agtgtatttt
     2041 gcagagtatg gatctttgag ctgctggcct actccaattt caacggcctc cacctcctct
     2101 ttggtcagct ccctcgcaga taaaactgag gaaaaaaaac aggaaaaaca aaaaacaaaa
     2161 aacaaaaaaa agtgactggt tttacctttc acattaaagc cctactatgt tgtccagaat
     2221 aaatcagcat acacagcctt atttaccata acaccgcatt acgcgcaccg ctcaaacgct
     2281 ccagaaacga ttctggggcg taacccatta aatactacgt cttaccccct gaaaacgcct
     2341 caaaatcgcg ctgatgggtt ttacgggcat gaattgttat gcaccagact ggctggagct
     2401 agatttcatc ctgaattcac gccagacaac tcaaaacccg caccaaaacc gactgaaaac
     2461 ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaacc
     2521 ggttttcagc acgttcagga atcgggttcg tcgacggccg gcaatgacgt aatggcaagt
     2581 cgccggacga ctcaccacac gattcaccgg cgattcgact cgtgactt
//
