LOCUS       CP052195                4052 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0014 plasmid pF16KP0014-3,
            complete sequence.
ACCESSION   CP052195
VERSION     CP052195.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609954
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:00:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,564
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 52 of 126
            Pseudo Genes (incomplete)         :: 68 of 126
            Pseudo Genes (internal stop)      :: 26 of 126
            Pseudo Genes (multiple problems)  :: 17 of 126
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0014"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0014-3"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            130..471
                     /locus_tag="HJX66_28380"
     CDS             130..471
                     /locus_tag="HJX66_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJO59556.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            434..1171
                     /locus_tag="HJX66_28385"
                     /pseudo=""
     CDS             434..1171
                     /locus_tag="HJX66_28385"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1149..1634
                     /locus_tag="HJX66_28390"
     CDS             1149..1634
                     /locus_tag="HJX66_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJO59553.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="mobilization protein"
     gene            1638..1850
                     /gene="mbeD"
                     /locus_tag="HJX66_28395"
     CDS             1638..1850
                     /gene="mbeD"
                     /locus_tag="HJX66_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJO59554.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="mbeD"
     gene            complement(2358..2816)
                     /locus_tag="HJX66_28400"
     CDS             complement(2358..2816)
                     /locus_tag="HJX66_28400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO59555.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          3916..4052
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.367"
                     /coverage="83.0"
                     /evalue="1.26e-45"
     Typing          complement(3453..3933)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          462..1966
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaagccac tgacgcgcct
       61 gcggcttgtc caacaccgtg ccgaccggga aacatttccc gcccgtcccg gtgttatcag
      121 gaggctactc tggcagacaa acgcaacaag atgctcacga tgtgggtgac ggaagacgaa
      181 caccggcggc tgctcgagcg gtgcgacggc aggcagctgg cggcgtggat gcgtcagtcc
      241 tgcctcgatg agaagccggc gcgtgcctgc agactgccgt caatatcgcc ggcgctgctg
      301 cgccagcttg ccggtatggg taacaacctg aaccagatcg cccggaaagt gaatgccgtt
      361 ggtgccggtc atgatcgggt gcagattgtg gccgccctga tggcaataga cgcgggactg
      421 gagcggctgc ggcatgcggt gctggaaaaa gggggggatg atgatcgtta aatttcatcc
      481 ccgggggcgc ggtggtggtg ccggtccggt ggattacctg ctgggaaaag accggcagcg
      541 cgagggggcg accgtgctgc agggaaaacc ggaggaagtc cgggagctta tcgatgcctc
      601 gccgtatgcg aaaaagtaca cctccggcgt gctgtcgttc gcggaggctg aactgctgcc
      661 cgggcagcgg gagcagatca tggccagctt tgagcgggtt ctgatgcccg ggctcgataa
      721 agaccagtac agcattctgt gggtggagca caccgacaag gggcggctgg agctgaattt
      781 cctgatcccc aacactgaac tgctgaccgg gaaacgcctg cagccgtatt acgatcgcgc
      841 cgaccggccg cgcatcgatg cctggcagac cgtggtgaac ggcagactgg gactgcatga
      901 tccaaacgcc ccggagaacc ggcggttact ggttacgccg tccgcactgc cggaaacgaa
      961 gctggaagcc gctcaggcga ttacacgtgg gttgctggcc ttagcctcat ccggggagct
     1021 caaaactcgc gaggacgtca caggtgcgct cacagcggca ggttttgagg tggtccgcac
     1081 tacaaaaaac agtatcagca ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg
     1141 agccatttat gagcagtctt ttagcgctgg cgaaggactt ggagcagcaa tcgaaagcgc
     1201 agcagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag aaatctgtaa
     1261 aagcggaact gagcgcaagc gcgaagagaa tcagcgacgc catcagcgcc cacgagcagg
     1321 gtatgaccgc ggcgatgcag tcgaaccgcc tgagcgtgct gcgtatggtc ggccggacgt
     1381 ggctgaccat caccctggtc tcggtactgc tgatcgccat gagcgggagc attctgtggt
     1441 ggcaggggca gcagatcacc gggaattacc agactatccg ggcgcaggag cggacgcagg
     1501 ccatgttgtc ggagaaaaac cacggcgttc agctgtcgct ctgcggggag aagaaactgt
     1561 cctgcgtcaa agtgaacccg aaagcgggag cttacgggga ggaagggaac tggatggtac
     1621 tggagaggaa atagcccatg acggaactgg aaaaacagtt gctgaccgca ttagagcagc
     1681 tacatcagga ctactcgcaa aggctggacg agtgggagag cgccttcgcg gaatggcaga
     1741 agatgtctgg tcttatgcaa cgggagaacg cggcgctgaa cgagcgcgtc acgcgcttga
     1801 gtcagcaggt ggcgaacttg agcaggcagt tacagcgttt gagccagtag tgcagcggca
     1861 tgaaatggcg gtggccgccg aacgggcata cgagcagcga cagcacgaaa aagagctggc
     1921 ggaagcccgt tcccgtcagc ggagctataa cggtccgacg ctgggctgac aggcttcacc
     1981 agtgacaaac cccacgaaga agggctcata aatttcccgc taaatacggt cgtgatgaaa
     2041 acatcacggc gtatacaacg gagggattat gagactgttt aacgtgattg agacggtgct
     2101 gaactgcctg tacatggcgc tggagatctg ggactggctg gagaagcatt ttcctgtttt
     2161 taaggcgttc atccagattc tggcctgaaa tcagaccgta tgcatctggc tgcatgacca
     2221 tgcagccggg taaaatttat ctgtcacgaa cggggcggtg tatcgggtgg tttgttgccg
     2281 gttttacctg actgccgccc cgtttgtgac gaacatgtcc ggagcggtgc gggcaagaga
     2341 tgttatggta aatcatatta gttctgaatg attcgggctc caccagtcat tactatttga
     2401 gcaccacctg acatttttat acttgtaccc tggctagtca ttgtcgtatc accttgtatg
     2461 atcgtttttc cagtagcatg ttgacctcca ccagtgacta aaattgacgc ttgtacttga
     2521 gatccattta tcaaaacagt tccggcttgg tgatcaaatt ttattccgct caatgctttt
     2581 aattcagctc catctagctg aatttcacca ttggctgctg cagcctgaat ggccccctgc
     2641 atctgagaaa ataatttatc aacttcgtca aaaggtaatg gtgtttcaag cagtgaggct
     2701 tgttgttcaa cttcaggatg tttacttgct aacttcccta tgatgccttt gattgcatca
     2761 gaagtaagat tgcttaatgc gccagtgaga acggcaagta gtgtggtttc aatcattata
     2821 tttccctcgt tagattgctg tgatgcgtat tatttactct ttataatctc cgattctgtc
     2881 catatggtta gttgcactat tacgtttata agtgccactt ttaccatata tatatccaac
     2941 gctacattgt ggacattttg gcgtctcgtc attatgagtg aaatttattt ctcctccgac
     3001 agatagggtt tttacgtttt ccggataatc taatgtagcc acgccggtcc attcacaatt
     3061 cgagcagaca acatcgaaag atgttgggat actcttgcag ttgatgttga tttgcatatg
     3121 cataacctcg attttgcaca gaaattacag tgttattagt tgcataccga ctataacctg
     3181 agtttgtgat ggagtggtgt ttttttgctt tgacttgagt aagagaaaga gatgatcttc
     3241 ttgagataat ctctttctga gcgtagtctt ttgctctgta aacgaaaaaa ccaccctggc
     3301 aggtggtttt tcgaaggtta gttaatcctg gcagatttac taaccgtggt aacagtcttg
     3361 tgcgagacat gtcaccaaag ctgtccttcc agtgtagccg cagtaaggcc accacttcaa
     3421 gaactctgaa tacatctctc gcacatcctg tttaccagtg gctactgcca atggcgattt
     3481 gtcgtgcctt ttcgggttgg actcaagacg atagttaccg aaaagagcgt ggtagtcgga
     3541 ctgaacgggg ggatcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     3601 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     3661 tggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata
     3721 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tttatgatac tcgtcagggg
     3781 ggcggagcct atggaaaaac ggcaacgccg catcctcttc ccggcttctc ctgatttgag
     3841 cgctgcttgc gtatcccgtc atctgtgagc tggtaacgcc cgccgcagtc tcacggcagg
     3901 agcgcagcga ctgagtgagc gaggaggcgt catttcacct ttaccagcat gttgcactta
     3961 cgcccgtcag ccacattttc cccttcccac atgaagcact ttcatacatt accactccgc
     4021 cgtaacatat ttagccagta tacactccgc ta
//
