LOCUS       CP052191                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0019 plasmid pF16KP0019-2,
            complete sequence.
ACCESSION   CP052191
VERSION     CP052191.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609955
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:26:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,433
            CDSs (total)                      :: 5,310
            Genes (coding)                    :: 5,160
            CDSs (with protein)               :: 5,160
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 77 of 150
            Pseudo Genes (incomplete)         :: 70 of 150
            Pseudo Genes (internal stop)      :: 28 of 150
            Pseudo Genes (multiple problems)  :: 23 of 150
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0019"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0019-2"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(9225..9294,1..350)
                     /gene="exc2"
                     /locus_tag="HJX67_27115"
     CDS             join(9225..9294,1..350)
                     /gene="exc2"
                     /locus_tag="HJX67_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJJ35952.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            656..919
                     /locus_tag="HJX67_27120"
     CDS             656..919
                     /locus_tag="HJX67_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJJ35953.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            909..1208
                     /locus_tag="HJX67_27125"
     CDS             909..1208
                     /locus_tag="HJX67_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJJ35954.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1270..1647)
                     /locus_tag="HJX67_27130"
     CDS             complement(1270..1647)
                     /locus_tag="HJX67_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJJ35955.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            1716..1895
                     /locus_tag="HJX67_27135"
     CDS             1716..1895
                     /locus_tag="HJX67_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJJ35956.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1923..2453)
                     /locus_tag="HJX67_27140"
     CDS             complement(1923..2453)
                     /locus_tag="HJX67_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJJ35957.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2460..3191)
                     /locus_tag="HJX67_27145"
     CDS             complement(2460..3191)
                     /locus_tag="HJX67_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJJ35958.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(3191..5155)
                     /locus_tag="HJX67_27150"
     CDS             complement(3191..5155)
                     /locus_tag="HJX67_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJJ35959.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6636..6785)
                     /locus_tag="HJX67_27155"
     CDS             complement(6636..6785)
                     /locus_tag="HJX67_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJJ35960.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6870..7127)
                     /locus_tag="HJX67_27160"
     CDS             complement(6870..7127)
                     /locus_tag="HJX67_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ35961.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(7137..8822)
                     /locus_tag="HJX67_27165"
     CDS             complement(7137..8822)
                     /locus_tag="HJX67_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJJ35962.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     BGC             2137..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5955..6533
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2464..3191)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ctccaaaggg cagcgtggaa cagcataccc ggcattacgt atatgcttca gatgacggtt
       61 ttgatcccaa tttttccacc cagaaagctg acacaacacg aatgatggtg ccttttttcc
      121 ggcagttctg ggatatgggc gcaaaggata aagcgaccgg aaaatcacgg agtgatgtgc
      181 agcaacgcat tcagcagttt cacagtcagg aatttttaaa ctcactccaa ggcacaacac
      241 aatttgcggg taccgattac cgcagcaaag acctcacacc gaaaaaatca aggctactgg
      301 ctgacaccat ttcagctgtt tatctcgatg gctatgaagg cagacagtaa gtggatttac
      361 cataacgcgt cattacgcgc accgctcaga cgggctcagg gcgtttccgg cggctacggg
      421 ataaaacagg caacaacccc cccggaaaac tgccggaaac gcgcccgata aattttatcc
      481 taatgcatgc ctatgcacgc aactgcatgg catatgtttt gacgtaaaat ggatgccgta
      541 agcccttttt taccgacttt ctgcttcatc agcctgactg acacgaccat attctttcag
      601 accgccccct gctaaacttc cttcatattg ccgcattaac ccgggaggct ccgcaatgag
      661 caacactaca cactacgaaa acgccaactt tcttcgggaa ctggctgaaa gcctgccccg
      721 catactgccg gaaggtggcc cggacaaagc tgcgctgttg cagcgactgg ctaacgaaga
      781 actggcccag gcagagtatg aagatcaggt tcgcgccaaa gtgaccgccg cccgtgctga
      841 cacgcgtccg ggtatgacca cggaacaact ccgccagcgc ctgcatggcc gttatcagga
      901 actgcgcgat gccgtatgac gtggaatgga agcagggagc catcgaggat gtaacgacgc
      961 tgtttgacta tatcgcggaa aattcatccc tgtgggatgc gcagaacgtt accgaccggg
     1021 ttctcgctgc ggcggacaaa ctggccgact tcccgcgact ctatgaagtg gactcacgct
     1081 atggcgagga cgttcgccgg atcagtctga tgggacagca tgtcctctat gatgttgatg
     1141 accagaccag aaaagtccgg gttctggccg tggtcggcca gcgccagaat ccccatattg
     1201 ttctctaata accggtgacg gggacgcaat tcattgcgtg gccgtcgccg gttggacaag
     1261 cacagcgcgt cagcgttttc cccacggcca ccaccggcgc ggtgttccgg ccggggtctc
     1321 tgctgtctcc ggtttgtgtt ccagcagtct catggactgc atttcctgcc gtagcccggc
     1381 cacctcctcc ctgagtgctt ttatctccgc gatcaacgcg tcggaggcca ttttttcagt
     1441 ctcacccgac agtctcaact cgtcccgctc gtgagtctca tcctgtctta actcaccgta
     1501 cacccggata agctcgctgg tatccaccac ccggccgcca tcagcttccg tacggtatga
     1561 tacgcggcct ttcgccatat cccgatacag ggaacttcgg gctttacccg tcagttccgt
     1621 cgcctgtctc accgtcaccc acgccattgt ctcaccccgc tatttttgag acagtatacc
     1681 tgacaggcgc acaccatgcc cggaaggaaa gaatcatgac aaaacaggaa aaaacagccc
     1741 tcaatatggc taaatttatt aaggagcagt cgctgctatt gctcgataaa ctgaatgagc
     1801 tggatatgga taccccggcc gacatgtgcg agcggctaca cgaggatgcg gaaacactct
     1861 ggcaggtgat gcgggataag ctcggggagg aataacagca ccggcatggc cggtgctggc
     1921 atttattttt tgccgaacgg ccaccaccgg cgggcaggtt tgtcctccgg cagttcaggt
     1981 accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt cagcttctcg
     2041 ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa tcatcgcgtt aatctgctcc
     2101 ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa ccgctccacc
     2161 ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt tgttccgttc ccattgcgga
     2221 tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc
     2281 ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa aacatacgcc
     2341 ccggtctgcc aggcgagcac caccatatct tccagatgct cgctggtcgg catgatgcag
     2401 cgctcgccgt tccggtcctc cgcgtcttca ataaatacca tgtacagcgc cataatccct
     2461 cactccgttg tcataaattt aaagcacgca aacaaccgtt cacgatccag ctctgccgtt
     2521 ttgcgggcct cgccggtctg tgggttcacg atttcatcca ccgacaggat tgcccgctgc
     2581 accctgacct gcgtcgcccg gtccgggcag atgtaccaga tttcatcgat gccgttagct
     2641 ttgcggttga acagataccc ggacaccact tcctgatacc ggcgacggct cttgacggtg
     2701 cgctccacct caaacgccac cgttttaccg tccagctccg ccagggcgtc cggcactttc
     2761 atgcctttaa gactcagacg gtgcagaatg cgccagccgg tggcccctct ggcttccata
     2821 tcaaggcgca gggattgcac ggccagccgg tgcggcagat gtgctgccga cacccttgac
     2881 ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt cgaacgagaa cgccacgcca
     2941 tgcggcgtca gcccccacac gctgacctga cggccgtcca ccggatattt ggtccggacc
     3001 agcagccctt tcgccaccag gctgtcgagg aagcggtaga ccggctgcac cgccgcaatc
     3061 cccagccgcc gcgcaaccag ctccgccgtg gaacacagct cttcccgcaa ccagcgcagg
     3121 gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg cgtggctgac gttgtacagc
     3181 tccagcgcca tcagtaaatc cccgcaaaat catcatccgc tttcggcccg ccgccgtcgg
     3241 ccgggggccg gaactccgcc aggggatcgg ttttcgccag caccgggaaa cgctgcaacg
     3301 ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc catccgggcc acacccagcc
     3361 cggtcatgac cgcgaacccg tccggcaggt gttgcagggt gttcacgtca aacagcggcc
     3421 gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc cacgttcccc gcctcccggc
     3481 tggcacggcg gcgctccacg tccaccctga tttccccggt ctgccccgcc gcccactgcg
     3541 cggactccag gctggccgcc cggtaaaacc agcggatcgg cgtgttatcc agtaccgtgg
     3601 ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc gaaatcgccc agcgactggt
     3661 gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc cagtgcgttc agtacatact
     3721 tcgacagcag atatttaatt tcatcgagca tcacgctgac gtgtggccac tcccggaact
     3781 cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag cattttttgc acacggatca
     3841 ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc gccgccgttg ataatccccg
     3901 ccacatcgat gccactgtcc gtctgcagga cgctcaggtc agcgacgttt tccagcctag
     3961 tgatcaaccc cttcacgtcc tttttcaggg cctccggcag cgcgtacgcc gccgccagca
     4021 cctgctgaat gtttgccccc tgcggaaact gctccgcaat cagcttcgcc gctttctgct
     4081 cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc caggttaagc ccggcaatca
     4141 gcaggttttt cagcgcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc
     4201 ccggccgcag atccagcagg gtgaagtccg gacagtgcgc ccggaagacg tgcggcgccc
     4261 atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc gaaatcgcgt acgcagcgaa
     4321 tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg caggccgagg atctgaatgt
     4381 tggtcttgcg ccaggtatca cgcggaacca gcaccggctt gccctgctca tccttaccca
     4441 gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa atcggccagc gcattaaccg
     4501 gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat ctctcctgtc cgctggctgc
     4561 tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcagcc gcaaaatgac
     4621 cgtcaagcag caggaacgcc agcagccgcg ccaacacaat cccgacagcc agcccgcccg
     4681 cgcaccaggc aatcccgaac aggttagccg gctgcagcag gtaataatct gcgtttgcgt
     4741 agaggtccgg acgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc
     4801 cccccagaag ggccagaccg gccgcgccgg tcagccagta cgcgtagttc cagacatgtc
     4861 gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc cagcaccgaa aatggcgctg
     4921 gccgtttatt atcgcgctta tagcgatcgg catactcccg tcgctcctta tgactcagcc
     4981 ccacaaaacg catccgtccg gcccaaagtt cagccagacc gaaaaacagg ccgataacta
     5041 ggaaagcccc ggccagaccg taatactcct ggtacagcca gctgacccac ggataaatct
     5101 gccgggccag ctccggaatg tgccacttcc aggcaagcga atccgtatta aacatgttct
     5161 ctctccttca gacgatggtt tgccggcgtg atgtgcagcc cccgctgcac aagccgcgga
     5221 catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca gctttcacca
     5281 cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg aacaacgagc
     5341 acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg
     5401 cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg agcagctttg
     5461 ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga
     5521 cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacggagcc agaaagaggg
     5581 ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca ggggatatcc ccctgaggtc
     5641 agccacgccc tcgcggggat gacctgtatt tctccgtaag gagaaaaaag caacagagat
     5701 aggttaacgt aaaatgttca gcgaaaaacc gatatgattc acctatcctg gtaatttatt
     5761 atcagggaat ataaagactt aatcagggga caatatgcca catcagcgcg tccggtgtcc
     5821 gtcttgtagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac gctccggccg
     5881 tgagactgcg gcgggcgctg cggacacatg caaagttacc cacagattcc gtgaataagc
     5941 aggggactaa catgtgaggc aaaacggcaa ggccacgcag gtggcgtttt tccataggct
     6001 ccgccctcct gactgagttc acataaacag acgctttttc ggtgcatctg tgggagccgg
     6061 tggctcaacc atgaatcagt caggagtggc gaaacccgac aggacttaaa gatcatcacc
     6121 gtttccgatg aatcgctccc tcttgcgctc tcctgttcca accctgccgt ttcccggata
     6181 cctgttccgc ctttctccct tgcgggaagt gtggcgcttt ctcatagctc acactctggt
     6241 atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ccccccgttc
     6301 agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg gaaaagcacg
     6361 caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta
     6421 aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg
     6481 gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc
     6541 ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt
     6661 tcagaggagg acgatggtgc caccgttcca ccctgaacat cccggatata gtttgcctga
     6721 catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa gaaaaacaac ctttaccttt
     6781 ttcatatacc ttccctgtat ttaaaaaccc cggtattcat ccctgaacac cggaagtctg
     6841 tatttcaaat catccttgat aacttttgct taccagttac catcccggta aacaaaggat
     6901 acaaagtaat caaatttact aatatcgatt acatttttaa agtgtggctg taatatcgaa
     6961 acccatgatt tttcaacatc aaaccaacca ttattaatat tatcctttaa aggcatcccc
     7021 agactttcaa tgacagaacc atcatcaccg aagtcttttg agtattcacc acctttaaac
     7081 tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa gccccatatt tacctcttaa
     7141 agatattttt taatgtttcg ttttggatcc ggccctttaa cctgcttacc cgtttttgga
     7201 tcgaatgccc cgaggtgttc gccatcactg gcgcggtacc cttcaagctc accgtgctgg
     7261 gaatcccatt cataaatttt acgcttttta tcaccatacc atcgggcccg cttaccacca
     7321 ccaccttgtt taggggtttt aggtttaccc tctttcaact ctcccaaccc cttaatatct
     7381 tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt tagttccctt acgaggtttt
     7441 ttcttttcct catttaattt attttctgca tccttcgctt ttttctcctt ttgtttccgg
     7501 ctctccatcg ccccggaaag cgcagcctta tccctggata aggattcatt catttcgttt
     7561 aaagatgtct gggcagcttt taatttctca ttctgattct gaatatcctt atcaagatag
     7621 cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt cacaaaatca
     7681 tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc agcccttgca
     7741 ccggggatcg ccttctctgt cgatgcaatg cgactgttta aggtcgcaat atttttatct
     7801 tcagcatcca gctcagcttt cgctttatca tactctcttt tcagcccctc ttccgggtga
     7861 gcagcgtccc atgcctgctg gcgacgcttt tcttcctcca gacgctgttt tacctgtgcc
     7921 ggagtaagaa catccgtcac cgacacatag accggtttct gtccgctctc tttcgggaaa
     7981 cgaataacgg cctcacggga gttaccaccg gccgtaaaac cagccggacg tgaatcacct
     8041 ttttcagcaa taatgccttt tggcggggct ttcgcggccg gaacaccttt aggtacgctc
     8101 acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg ttttgtcggt
     8161 ttcgcatcca ccacaggaac actcattggc cggccggtga caaccgcaat atgctgccgc
     8221 tcgtccttca caatatccgc cacacgttta gtgacgctga ccgtcgcctg gtccagcggc
     8281 agggagctga ccggcgtctc cgttaccgtg tctgccggca atgacgtcac gatttttgac
     8341 atcattttcg gatcatcttt tgctatctca gaaggcagca ctccgtaaat cgcaatcccc
     8401 cacagaccaa acttaaacgg acctttcagg gcagccagca catcagcaac agcggccgac
     8461 aacgcatcgc cggaaacgga taaagccagt ccttcagccc ccggagtggc cagggcaggc
     8521 agaccgaagg ccatcgcggt ggccgaagac tgtccgccac cagagctccc gctatgattg
     8581 ccgctgttac cgccattacc accgggttta ctcccgccgt taccaaactc ccccgggtta
     8641 tagttatgaa tatccccgtt aggtgtatgc gtcgtacccc agcccggccc gctgtttgga
     8701 tccgtaccac cacctgatga agggccacct ttacctgatg taccgttcac attcccactg
     8761 gcctgaccac cattgtgtcc cagacctgaa ttacccggac ctcgtccgtc tccgccactc
     8821 atatactttt cctctttgac ttttaaaaca ataaactaaa ataaaatact gtacatataa
     8881 ccactggttt tatatacagt ataaaaatta cgctgccttg tttttcatgt caagtctgta
     8941 aatttgcgtt tttgtgaaaa tgatcaaaaa aacagccact cacaccatgc gcaatcccct
     9001 ccagatgcaa caaaaaccag cacaaacggc gccgttctcc ggcctcagta tgttttactg
     9061 aaatcattat atgagatacc agaaagcccc ctccgggaca ggaaaagaaa cggggggtct
     9121 ggttcgggtt gttcggtcac tgaacacggt cagaaactga tgataagata gcctgcggat
     9181 atataactca atgagaaaaa agaattaaag gtaaggagac gctcatgaga cgttccggca
     9241 gcaaactgtg tcttatcgcc atgataacag cgacagcaac tctcactgcc tgtg
//
