LOCUS       CP052180                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0045 plasmid pF16KP0045-2,
            complete sequence.
ACCESSION   CP052180
VERSION     CP052180.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609958
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:00:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,397
            CDSs (total)                      :: 5,275
            Genes (coding)                    :: 5,129
            CDSs (with protein)               :: 5,129
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 73 of 146
            Pseudo Genes (incomplete)         :: 72 of 146
            Pseudo Genes (internal stop)      :: 28 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0045"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0045-2"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(7674..9294,1..344))
                     /locus_tag="HJX70_26935"
     CDS             complement(join(7674..9294,1..344))
                     /locus_tag="HJX70_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJO32634.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRMDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1825..1974)
                     /locus_tag="HJX70_26940"
     CDS             complement(1825..1974)
                     /locus_tag="HJX70_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJO32635.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2059..2316)
                     /locus_tag="HJX70_26945"
     CDS             complement(2059..2316)
                     /locus_tag="HJX70_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO32636.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(2326..4011)
                     /locus_tag="HJX70_26950"
     CDS             complement(2326..4011)
                     /locus_tag="HJX70_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJO32637.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            4414..4833
                     /gene="exc2"
                     /locus_tag="HJX70_26955"
     CDS             4414..4833
                     /gene="exc2"
                     /locus_tag="HJX70_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJO32638.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            5139..5402
                     /locus_tag="HJX70_26960"
     CDS             5139..5402
                     /locus_tag="HJX70_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJO32639.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            5392..5691
                     /locus_tag="HJX70_26965"
     CDS             5392..5691
                     /locus_tag="HJX70_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJO32640.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5753..6130)
                     /locus_tag="HJX70_26970"
     CDS             complement(5753..6130)
                     /locus_tag="HJX70_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJO32641.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            6199..6378
                     /locus_tag="HJX70_26975"
     CDS             6199..6378
                     /locus_tag="HJX70_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJO32642.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6406..6936)
                     /locus_tag="HJX70_26980"
     CDS             complement(6406..6936)
                     /locus_tag="HJX70_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJO32643.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6943..7674)
                     /locus_tag="HJX70_26985"
     CDS             complement(6943..7674)
                     /locus_tag="HJX70_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJO32644.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     BGC             1..9011
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1144..1722
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6947..7674)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gccagaccgg ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg
       61 accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta
      121 tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc
      181 atccgtccgg cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg
      241 gccagaccgt aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc
      301 tccggaatgt gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag
      361 acgatggttt gccggcgtga tgtgcagccc ccgctgcaca agccgcggac atgccccaag
      421 gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc
      481 ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg
      541 tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga
      601 gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca
      661 ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg
      721 tgaatttctg gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt
      781 gcgtcggtgg cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct
      841 cgcggggatg acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta
      901 aaatgttcag cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata
      961 taaagactta atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt
     1021 gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg
     1081 cgggcgctgc ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac
     1141 atgtgaggca aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg
     1201 actgagttca cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca
     1261 tgaatcagtc aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga
     1321 atcgctccct cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc
     1381 tttctccctt gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg
     1441 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc
     1501 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac
     1561 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
     1621 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
     1681 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
     1741 cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat
     1801 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga
     1861 cgatggtgcc accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag
     1921 aagaaagcct gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct
     1981 tccctgtatt taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc
     2041 atccttgata acttttgctt accagttacc atcccggtaa acaaaggata caaagtaatc
     2101 aaatttacta atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt
     2161 ttcaacatca aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat
     2221 gacagaacca tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt
     2281 cttatcaaac caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt
     2341 aatgtttcgt tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc
     2401 gaggtgttcg ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc
     2461 ataaatttta cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt
     2521 aggggtttta ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg
     2581 atcaggaaaa taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc
     2641 atttaattta ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc
     2701 cccggaaagc gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg
     2761 ggcagctttt aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc
     2821 ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc
     2881 ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc
     2941 cttctctgtc gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag
     3001 ctcagctttc gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca
     3061 tgcctgctgg cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaagaac
     3121 atccgtcacc gacacataga ccggtttctg tccgctctct ttcgggaaac gaataacggc
     3181 ctcacgggag ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt tttcagcaat
     3241 aatgcctttt ggcggggctt tcgcggccgg aacaccttta ggtacgctca cctgcagaga
     3301 cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac
     3361 cacaggaaca ctcattggcc ggccggtgac aaccgcaata tgctgccgct cgtccttcac
     3421 aatatccgcc acacgtttag tgacgctgac cgtcgcctgg tccagcggca gggagctgac
     3481 cggcgtctcc gttaccgtgt ctgccggcaa tgacgtcacg atttttgaca tcattttcgg
     3541 atcatctttt gctatctcag aaggcagcac tccgtaaatc gcaatccccc acagaccaaa
     3601 cttaaacgga cctttcaggg cagccagcac atcagcaaca gcggccgaca acgcatcgcc
     3661 ggaaacggat aaagccagtc cttcagcccc cggagtggcc agggcaggca gaccgaaggc
     3721 catcgcggtg gccgaagact gtccgccacc agagctcccg ctatgattgc cgctgttacc
     3781 gccattacca ccgggtttac tcccgccgtt accaaactcc cccgggttat agttatgaat
     3841 atccccgtta ggtgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc
     3901 acctgatgaa gggccacctt tacctgatgt accgttcaca ttcccactgg cctgaccacc
     3961 attgtgtccc agacctgaat tacccggacc tcgtccgtct ccgccactca tatacttttc
     4021 ctctttgact tttaaaacaa taaactaaaa taaaatactg tacatataac cactggtttt
     4081 atatacagta taaaaattac gctgccttgt ttttcatgtc aagtctgtaa atttgcgttt
     4141 ttgtgaaaat gatcaaaaaa acagccactc acaccatgcg caatcccctc cagatgcaac
     4201 aaaaaccagc acaaacggcg ccgttctccg gcctcagtat gttttactga aatcattata
     4261 tgagatacca gaaagccccc tccgggacag gaaaagaaac ggggggtctg gttcgggttg
     4321 ttcggtcact gaacacggtc agaaactgat gataagatag cctgcggata tataactcaa
     4381 tgagaaaaaa gaattaaagg taaggagacg ctcatgagac gttccggcag caaactgtgt
     4441 cttatcgcca tgataacagc gacagcaact ctcactgcct gtgctccaaa gggcagcgtg
     4501 gaacagcata cccggcatta cgtatatgct tcagatgacg gttttgatcc caatttttcc
     4561 acccagaaag ctgacacaac acgaatgatg gtgccttttt tccggcagtt ctgggatatg
     4621 ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg tgcagcaacg cattcagcag
     4681 tttcacagtc aggaattttt aaactcactc caaggcacaa cacaatttgc gggtaccgat
     4741 taccgcagca aagacctcac accgaaaaaa tcaaggctac tggctgacac catttcagct
     4801 gtttatctcg atggctatga aggcagacag taagtggatt taccataacg cgtcattacg
     4861 cgcaccgctc agacgggctc agggcgtttc cggcggctac gggataaaac aggcaacaac
     4921 ccccccggaa aactgccgga aacgcgcccg ataaatttta tcctaatgca tgcctatgca
     4981 cgcaactgca tggcatatgt tttgacgtaa aatggatgcc gtaagccctt ttttaccgac
     5041 tttctgcttc atcagcctga ctgacacgac catattcttt cagaccgccc cctgctaaac
     5101 ttccttcata ttgccgcatt aacccgggag gctccgcaat gagcaacact acacactacg
     5161 aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc ccgcatactg ccggaaggtg
     5221 gcccggacaa agctgcgctg ttgcagcgac tggctaacga agaactggcc caggcagagt
     5281 atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga
     5341 ccacggaaca actccgccag cgcctgcatg gccgttatca ggaactgcgc gatgccgtat
     5401 gacgtggaat ggaagcaggg agccatcgag gatgtaacga cgctgtttga ctatatcgcg
     5461 gaaaattcat ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac
     5521 aaactggccg acttcccgcg actctatgaa gtggactcac gctatggcga ggacgttcgc
     5581 cggatcagtc tgatgggaca gcatgtcctc tatgatgttg atgaccagac cagaaaagtc
     5641 cgggttctgg ccgtggtcgg ccagcgccag aatccccata ttgttctcta ataaccggtg
     5701 acggggacgc aattcattgc gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt
     5761 ttccccacgg ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc tccggtttgt
     5821 gttccagcag tctcatggac tgcatttcct gccgtagccc ggccacctcc tccctgagtg
     5881 cttttatctc cgcgatcaac gcgtcggagg ccattttttc agtctcaccc gacagtctca
     5941 actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg ataagctcgc
     6001 tggtatccac cacccggccg ccatcagctt ccgtacggta tgatacgcgg cctttcgcca
     6061 tatcccgata cagggaactt cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca
     6121 cccacgccat tgtctcaccc cgctattttt gagacagtat acctgacagg cgcacaccat
     6181 gcccggaagg aaagaatcat gacaaaacag gaaaaaacag ccctcaatat ggctaaattt
     6241 attaaggagc agtcgctgct attgctcgat aaactgaatg agctggatat ggataccccg
     6301 gccgacatgt gcgagcggct acacgaggat gcggaaacac tctggcaggt gatgcgggat
     6361 aagctcgggg aggaataaca gcaccggcat ggccggtgct ggcatttatt ttttgccgaa
     6421 cggccaccac cggcgggcag gtttgtcctc cggcagttca ggtaccggca cgttaaaata
     6481 gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc
     6541 gtgccgctca ctcaccacgt caatcatcgc gttaatctgc tccggtgagc acttcccggc
     6601 atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc accttgcgat agaggtaatc
     6661 acacagcttc attcctgtgg gtttgttccg ttcccattgc ggatttttac cagtgaaaaa
     6721 gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat
     6781 cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag
     6841 caccaccata tcttccagat gctcgctggt cggcatgatg cagcgctcgc cgttccggtc
     6901 ctccgcgtct tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa
     6961 tttaaagcac gcaaacaacc gttcacgatc cagctctgcc gttttgcggg cctcgccggt
     7021 ctgtgggttc acgatttcat ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc
     7081 ccggtccggg cagatgtacc agatttcatc gatgccgtta gctttgcggt tgaacagata
     7141 cccggacacc acttcctgat accggcgacg gctcttgacg gtgcgctcca cctcaaacgc
     7201 caccgtttta ccgtccagct ccgccagggc gtccggcact ttcatgcctt taagactcag
     7261 acggtgcaga atgcgccagc cggtggcccc tctggcttcc atatcaaggc gcagggattg
     7321 cacggccagc cggtgcggca gatgtgctgc cgacaccctt gacggctgaa acggaataac
     7381 atccgccagc ggttcgtcct cgtcgaacga gaacgccacg ccatgcggcg tcagccccca
     7441 cacgctgacc tgacggccgt ccaccggata tttggtccgg accagcagcc ctttcgccac
     7501 caggctgtcg aggaagcggt agaccggctg caccgccgca atccccagcc gccgcgcaac
     7561 cagctccgcc gtggaacaca gctcttcccg caaccagcgc agggtcagcc gggttttctc
     7621 cgcgttccgc gcctgtcgtt tcgcgtggct gacgttgtac agctccagcg ccatcagtaa
     7681 atccccgcaa aatcatcatc cgctttcggc ccgccgccgt cggccggggg ccggaactcc
     7741 gccaggggat cggttttcgc cagcaccggg aaacgctgca acgggatttc ccggcgctca
     7801 acccgcagcg gactgctgaa cgccatccgg gccacaccca gcccggtcat gaccgcgaac
     7861 ccgtccggca ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg
     7921 aagctgtccc cgctgatatg ctccacgttc cccgcctccc ggctggcacg gcggcgctcc
     7981 acgtccatcc tgatttcccc ggtctgcccc gccgcccact gcgcggactc caggctggcc
     8041 gcccggtaaa accagcggat cggcgtgtta tccagtaccg tggttttcac aaattcagcg
     8101 ggcagatcct gcccgcactg cccgaaatcg cccagcgact ggtgcgcgag caacagattg
     8161 cagtcccggc tgcgcagcgt ccccagtgcg ttcagtacat acttcgacag cagatattta
     8221 atttcatcga gcatcacgct gacgtgtggc cactcccgga actcgtcccg cgcaatgatg
     8281 atttgcgcgc agcgggcaaa cagcattttt tgcacacgga tcaccgcctc gtcgtccatc
     8341 gagccgataa cgtacaggca gccgccgccg ttgataatcc ccgccacatc gatgccactg
     8401 tccgtctgca ggacgctcag gtcagcgacg ttttccagcc tagtgatcaa ccccttcacg
     8461 tcctttttca gggcctccgg cagcgcgtac gccgccgcca gcacctgctg aatgtttgcc
     8521 ccctgcggaa actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg
     8581 tccgccacgt cgccggtctc gcccaggtta agcccggcaa tcagcaggtt tttcagcgcg
     8641 tactggtcga gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc
     8701 agggtgaagt ccggacagtg cgcccggaag acgtgcggcg cccatgcgtc ccctttgggg
     8761 tcgaagtaca ccaccgcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc
     8821 atcacgctct tgccgctccc gggcaggccg aggatctgaa tgttggtctt gcgccaggta
     8881 tcacgcggaa ccagcaccgg cttgccctgc tcatccttac ccagaaacac cgcctgctcc
     8941 tgccgggcct gctccgagga aaaatcggcc agcgcattaa ccggtaccgg ctccccgaaa
     9001 tgcaggtggc gcacgtcggt catctctcct gtccgctggc tgctgcgccg gctctcctga
     9061 ttcagtttcg cgttcaccgc ctcgtcagca gccgcaaaat gaccgtcaag cagcaggaac
     9121 gccagcagcc gcgccaacac aatcccgaca gccagcccgc ccgcgcacca ggcaatcccg
     9181 aacaggttag ccggctgcag caggtaataa tctgcgtttg cgtagaggtc cggacgcagg
     9241 acggtttctc cccagctctc gataaacacg gcgacaacca gcccccccag aagg
//
