LOCUS       CP052174                2804 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0064 plasmid pF16KP0064-2,
            complete sequence.
ACCESSION   CP052174
VERSION     CP052174.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609961
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 21:09:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,332
            Genes (coding)                    :: 5,227
            CDSs (with protein)               :: 5,227
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 59 of 105
            Pseudo Genes (internal stop)      :: 22 of 105
            Pseudo Genes (multiple problems)  :: 17 of 105
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0064"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0064-2"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2365..2804,1..304))
                     /locus_tag="HJX73_27265"
     CDS             complement(join(2365..2804,1..304))
                     /locus_tag="HJX73_27265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="QJJ03861.1"
                     /translation="MGNSKRNIKKLNDNFRESILDYAISHNLKCANALIALYATGCRPD
                     EIETGIKVNFDSKNNKLSFKIIGSKLNYQQKRGIRIREVTIKITDKNLQYYKPIIDIFE
                     KNPQAYDLKIKTESSKAFSGYITKISKKLWPRKKHHVSAYSFRHAKATELKNSENFDKE
                     EIAKIMGHASIRSQESYGRKNSRSKGGFDDITDVETSSKPRGGDRLLRFKIASKQQTAA
                     KIAAISTPPDVASPPPTAPVRSLKR"
                     /name="site-specific integrase"
     gene            complement(316..1029)
                     /locus_tag="HJX73_27270"
     CDS             complement(316..1029)
                     /locus_tag="HJX73_27270"
                     /inference="COORDINATES: protein motif:HMM:NF013122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="QJJ03859.1"
                     /translation="MATKIENDEVIPDEELNKIIEEINNEEDKRIKVIVDDYQQLTQNG
                     FFVLDTETTDLVGEVVSFSLIDDKNNILINEYCKPTRNKISKTAQSIHNISNKMIKDSR
                     NIISIYEDFLEIINREENKNKTIFIYNEGFDLSTIYRSLNYAGYKYKENNDFDDVEFRS
                     RCVMKDFAYYYGEHNEYYDTDKYKWKKLEFAANYFNIETKNLELHNSAVDCYLTLKVIE
                     NIRDHFKKENEYFSV"
                     /name="3'-5' exonuclease"
     gene            complement(1409..1903)
                     /locus_tag="HJX73_27275"
     CDS             complement(1409..1903)
                     /locus_tag="HJX73_27275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QJJ03860.1"
                     /translation="MGLAQVKALDNIQEFEFDDEVAEQVQGLQEKGNVKVTAIIQPEKK
                     NSLKSEFVMAFTSNLRALAELNISQSELKVITYILEIMEYGNLISLNQSQIARDLDMKK
                     SNMSVIFKKLTEKGVLIKEKGHLFMNSNLFAKGLNHKLNTERRENLKTAQFENDKFTRS
                     F"
                     /name="MarR family transcriptional regulator"
ORIGIN
        1 tatctattat aggtttgtaa tattgaagat ttttatctgt gatttttatc gtaacttctc
       61 taattctaat acctcttttt tgttgatagt ttaattttga acctataatt ttgaaactta
      121 acttattgtt tttactatca aaattaactt taattccggt ttctatttcg tctggtctac
      181 acccagtcgc atataatgcg atcaaggcgt tagcgcattt taggttatgc gaaatcgcat
      241 aatctaaaat actctctcga aaattatcat ttagtttttt tatatttctt tttgaattac
      301 ccatattttt ttcacctaca cagaaaaata ctcgttttct tttttaaaat gatctctaat
      361 gttttcaatt acttttaaag tcaaataaca atcgacggcg gaattatgca attctaagtt
      421 tttcgtttcg atattaaaat aatttgctgc gaattcgagt tttttccatt tatatttatc
      481 tgtatcatag tactcattat gttctccgta ataataggca aagtctttca ttacgcaacg
      541 agatctaaat tctacatcgt cgaaatcgtt attttcttta tatttatatc cggcataatt
      601 taaagatcta taaattgttg aaagatcaaa accctcatta taaataaaaa tagttttatt
      661 tttattttct tctctattta ttatttctaa aaaatcttcg tatattgaaa ttatatttct
      721 actatctttg atcattttat ttgaaatatt atgtattgac tgtgctgttt ttgaaatctt
      781 atttctagta ggtttgcaat attcatttat taatatattg tttttgtcgt caatcaatga
      841 aaaactaaca acttcaccaa caagatctgt tgtttccgta tcaagaacga aaaaaccgtt
      901 ttgcgtaagt tgttgatagt catcaacaat tactttaatt cttttatctt cctcattgtt
      961 tatttcttct attattttat ttaattcctc atctggaatt acttcgtcat tttcaatttt
     1021 tgttgccata atttcacctc atttattgtt ttttatattg ttaacatcaa aatccggaga
     1081 tttcaaaaat tcgctacgct caaatttgaa ataccgtaaa acccaaacca tccccccata
     1141 ttaaatttat cgctctcgct caaaattcaa tatgaccccc tatgtaaggg ggtggcctta
     1201 tttcaatatg acgttgtcat atctcataat gccgtgttgt tccaccccag gaacggggtt
     1261 gaaacaacaa aagataatat agatataacc cggtggaaat taatgtcaat atctcaaata
     1321 tggagaaaat aagaataaaa aaaatccctt agaaaataga atttttttct attttttaag
     1381 ggatttggcc cgtccggcga ggacggtttt aaaatgatct tgtgaattta tcgttttcaa
     1441 attgtgcagt ttttaaattc tctcttcgtt ctgtatttaa tttatgattt aaacccttag
     1501 caaatagatt agaattcata aacaaatgtc ctttttcttt tattaaaact cccttctcag
     1561 tgagtttctt aaaaataaca gacatattag attttttcat atctaaatct ctcgcaatct
     1621 gcgattgatt tagactaatt agattcccgt actccattat ttctaaaatg tacgtaatta
     1681 cttttaattc gctttgacta atatttaatt ctgctaatgc tcttaaatta cttgtaaaag
     1741 ccataacaaa ttcgcttttg agcgaattct ttttctcagg ttgtataata gcagtgactt
     1801 ttacatttcc tttttcttgt aaaccttgta cctgttctgc gacctcatca tcgaattcaa
     1861 attcttgaat attatcaagt gctttaactt gtgctaaccc cataattttt ttactccttt
     1921 ttaaattgta taaactcagc tcttttataa tatattttgt gtactagtca ataacaaagt
     1981 aaaaaaaaat taaactttgt tatatgtctt tggactatag tccaatcctg atagttatga
     2041 gatctcataa ctggagttat gagtttacat aacttcgagt tataagatta cataactgga
     2101 gttatgagtt tacataactt ttttttgaaa aaaactatat ataacaatgt cttgatagta
     2161 tgttaagaag taagaattaa taagaacata agaaaggaaa acaatccagg ctgtgccagt
     2221 cgatagatcc aaaaaaccca tctgaaaaac catcccagtt acccctccgg ggctctgctt
     2281 cgcaatatgg aggattttgc tataaaaacc gtcctggccg gacgggccaa cacttcaaag
     2341 ccacacagca agctgtgtag cttctcagcg ttttaatgaa cgaacgggag ccgttggagg
     2401 tggacttgct acatctggag gggtggaaat agccgcgatt tttgcggctg tctgctgttt
     2461 actggcaatc ttgaaacgaa gcaatctatc gccaccacgg ggcttagagc ttgtttcaac
     2521 atctgtaatg tcgtcaaatc cacctttgct tctgctgttc tttcttccgt aactttcttg
     2581 agatctgatc gaggcatggc ccataatttt agcgatttct tccttatcaa aattctctga
     2641 gttttttaat tctgttgctt ttgcatgtct aaaactatat gcagaaacat gatgtttttt
     2701 tctgggccaa agttttttac ttatttttgt aatatatccg gagaatgctt tagaactttc
     2761 agttttaatt tttaaatcat atgcctgagg gtttttctca aaaa
//
