LOCUS       CP052166                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0082 plasmid pF16KP0082-4,
            complete sequence.
ACCESSION   CP052166
VERSION     CP052166.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609964
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:12:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,617
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 86 of 169
            Pseudo Genes (incomplete)         :: 80 of 169
            Pseudo Genes (internal stop)      :: 36 of 169
            Pseudo Genes (multiple problems)  :: 31 of 169
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0082"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0082-4"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(8071..9294,1..741)
                     /locus_tag="HJX76_28665"
     CDS             join(8071..9294,1..741)
                     /locus_tag="HJX76_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJN95232.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            741..1472
                     /locus_tag="HJX76_28670"
     CDS             741..1472
                     /locus_tag="HJX76_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJN95233.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            1479..2009
                     /locus_tag="HJX76_28675"
     CDS             1479..2009
                     /locus_tag="HJX76_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN95234.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2037..2216)
                     /locus_tag="HJX76_28680"
     CDS             complement(2037..2216)
                     /locus_tag="HJX76_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJN95235.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2285..2662
                     /locus_tag="HJX76_28685"
     CDS             2285..2662
                     /locus_tag="HJX76_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJN95236.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2724..3023)
                     /locus_tag="HJX76_28690"
     CDS             complement(2724..3023)
                     /locus_tag="HJX76_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJN95237.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3013..3276)
                     /locus_tag="HJX76_28695"
     CDS             complement(3013..3276)
                     /locus_tag="HJX76_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJN95238.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(3582..4001)
                     /gene="exc2"
                     /locus_tag="HJX76_28700"
     CDS             complement(3582..4001)
                     /gene="exc2"
                     /locus_tag="HJX76_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJN95239.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4404..6089
                     /locus_tag="HJX76_28705"
     CDS             4404..6089
                     /locus_tag="HJX76_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJN95240.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            6099..6356
                     /locus_tag="HJX76_28710"
     CDS             6099..6356
                     /locus_tag="HJX76_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJN95241.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            6441..6590
                     /locus_tag="HJX76_28715"
     CDS             6441..6590
                     /locus_tag="HJX76_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJN95242.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6694..7272)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          742..1469
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gtcctgcaga cggacagtgg catcgatgtg gcggggatta tcaacggcgg cggctgcctg
       61 tacgttatcg gctcgatgga cgacgaggcg gtgatccgtg tgcaaaaaat gctgtttgcc
      121 cgctgcgcgc aaatcatcat tgcgcgggac gagttccggg agtggccaca cgtcagcgtg
      181 atgctcgatg aaattaaata tctgctgtcg aagtatgtac tgaacgcact ggggacgctg
      241 cgcagccggg actgcaatct gttgctcgcg caccagtcgc tgggcgattt cgggcagtgc
      301 gggcaggatc tgcccgctga atttgtgaaa accacggtac tggataacac gccgatccgc
      361 tggttttacc gggcggccag cctggagtcc gcgcagtggg cggcggggca gaccggggaa
      421 atcagggtgg acgtggagcg ccgccgtgcc agccgggagg cggggaacgt ggagcatatc
      481 agcggggaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa
      541 cacctgccgg acgggttcgc ggtcatgacc gggctgggtg tggcccggat ggcgttcagc
      601 agtccgctgc gggttgagcg ccgggaaatc ccgttgcagc gtttcccggt gctggcgaaa
      661 accgatcccc tggcggagtt ccggcccccg gccgacggcg gcgggccgaa agcggatgat
      721 gattttgcgg ggatttactg atggcgctgg agctgtacaa cgtcagccac gcgaaacgac
      781 aggcgcggaa cgcggagaaa acccggctga ccctgcgctg gttgcgggaa gagctgtgtt
      841 ccacggcgga gctggttgcg cggcggctgg ggattgcggc ggtgcagccg gtctaccgct
      901 tcctcgacag cctggtggcg aaagggctgc tggtccggac caaatatccg gtggacggcc
      961 gtcaggtcag cgtgtggggg ctgacgccgc atggcgtggc gttctcgttc gacgaggacg
     1021 aaccgctggc ggatgttatt ccgtttcagc cgtcaagggt gtcggcagca catctgccgc
     1081 accggctggc cgtgcaatcc ctgcgccttg atatggaagc cagaggggcc accggctggc
     1141 gcattctgca ccgtctgagt cttaaaggca tgaaagtgcc ggacgccctg gcggagctgg
     1201 acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa gagccgtcgc cggtatcagg
     1261 aagtggtgtc cgggtatctg ttcaaccgca aagctaacgg catcgatgaa atctggtaca
     1321 tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc aatcctgtcg gtggatgaaa
     1381 tcgtgaaccc acagaccggc gaggcccgca aaacggcaga gctggatcgt gaacggttgt
     1441 ttgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac atggtattta
     1501 ttgaagacgc ggaggaccgg aacggcgagc gctgcatcat gccgaccagc gagcatctgg
     1561 aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg
     1621 cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc aacagcgacg
     1681 aaaaagccac gtactttttc actggtaaaa atccgcaatg ggaacggaac aaacccacag
     1741 gaatgaagct gtgtgattac ctctatcgca aggtggagcg gttcgccatc gagaacggtg
     1801 tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg attgacgtgg
     1861 tgagtgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag ctgatgcggg
     1921 aatataacag ccgctatttt aacgtgccgg tacctgaact gccggaggac aaacctgccc
     1981 gccggtggtg gccgttcggc aaaaaataaa tgccagcacc ggccatgccg gtgctgttat
     2041 tcctccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc gtgtagccgc
     2101 tcgcacatgt cggccggggt atccatatcc agctcattca gtttatcgag caatagcagc
     2161 gactgctcct taataaattt agccatattg agggctgttt tttcctgttt tgtcatgatt
     2221 ctttccttcc gggcatggtg tgcgcctgtc aggtatactg tctcaaaaat agcggggtga
     2281 gacaatggcg tgggtgacgg tgagacaggc gacggaactg acgggtaaag cccgaagttc
     2341 cctgtatcgg gatatggcga aaggccgcgt atcataccgt acggaagctg atggcggccg
     2401 ggtggtggat accagcgagc ttatccgggt gtacggtgag ttaagacagg atgagactca
     2461 cgagcgggac gagttgagac tgtcgggtga gactgaaaaa atggcctccg acgcgttgat
     2521 cgcggagata aaagcactca gggaggaggt ggccgggcta cggcaggaaa tgcagtccat
     2581 gagactgctg gaacacaaac cggagacagc agagaccccg gccggaacac cgcgccggtg
     2641 gtggccgtgg ggaaaacgct gacgcgctgt gcttgtccaa ccggcgacgg ccacgcaatg
     2701 aattgcgtcc ccgtcaccgg ttattagaga acaatatggg gattctggcg ctggccgacc
     2761 acggccagaa cccggacttt tctggtctgg tcatcaacat catagaggac atgctgtccc
     2821 atcagactga tccggcgaac gtcctcgcca tagcgtgagt ccacttcata gagtcgcggg
     2881 aagtcggcca gtttgtccgc cgcagcgaga acccggtcgg taacgttctg cgcatcccac
     2941 agggatgaat tttccgcgat atagtcaaac agcgtcgtta catcctcgat ggctccctgc
     3001 ttccattcca cgtcatacgg catcgcgcag ttcctgataa cggccatgca ggcgctggcg
     3061 gagttgttcc gtggtcatac ccggacgcgt gtcagcacgg gcggcggtca ctttggcgcg
     3121 aacctgatct tcatactctg cctgggccag ttcttcgtta gccagtcgct gcaacagcgc
     3181 agctttgtcc gggccacctt ccggcagtat gcggggcagg ctttcagcca gttcccgaag
     3241 aaagttggcg ttttcgtagt gtgtagtgtt gctcattgcg gagcctcccg ggttaatgcg
     3301 gcaatatgaa ggaagtttag cagggggcgg tctgaaagaa tatggtcgtg tcagtcaggc
     3361 tgatgaagca gaaagtcggt aaaaaagggc ttacggcatc cattttacgt caaaacatat
     3421 gccatgcagt tgcgtgcata ggcatgcatt aggataaaat ttatcgggcg cgtttccggc
     3481 agttttccgg gggggttgtt gcctgtttta tcccgtagcc gccggaaacg ccctgagccc
     3541 gtctgagcgg tgcgcgtaat gacgcgttat ggtaaatcca cttactgtct gccttcatag
     3601 ccatcgagat aaacagctga aatggtgtca gccagtagcc ttgatttttt cggtgtgagg
     3661 tctttgctgc ggtaatcggt acccgcaaat tgtgttgtgc cttggagtga gtttaaaaat
     3721 tcctgactgt gaaactgctg aatgcgttgc tgcacatcac tccgtgattt tccggtcgct
     3781 ttatcctttg cgcccatatc ccagaactgc cggaaaaaag gcaccatcat tcgtgttgtg
     3841 tcagctttct gggtggaaaa attgggatca aaaccgtcat ctgaagcata tacgtaatgc
     3901 cgggtatgct gttccacgct gccctttgga gcacaggcag tgagagttgc tgtcgctgtt
     3961 atcatggcga taagacacag tttgctgccg gaacgtctca tgagcgtctc cttaccttta
     4021 attctttttt ctcattgagt tatatatccg caggctatct tatcatcagt ttctgaccgt
     4081 gttcagtgac cgaacaaccc gaaccagacc ccccgtttct tttcctgtcc cggagggggc
     4141 tttctggtat ctcatataat gatttcagta aaacatactg aggccggaga acggcgccgt
     4201 ttgtgctggt ttttgttgca tctggagggg attgcgcatg gtgtgagtgg ctgttttttt
     4261 gatcattttc acaaaaacgc aaatttacag acttgacatg aaaaacaagg cagcgtaatt
     4321 tttatactgt atataaaacc agtggttata tgtacagtat tttattttag tttattgttt
     4381 taaaagtcaa agaggaaaag tatatgagtg gcggagacgg acgaggtccg ggtaattcag
     4441 gtctgggaca caatggtggt caggccagtg ggaatgtgaa cggtacatca ggtaaaggtg
     4501 gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt acgacgcata
     4561 cacctaacgg ggatattcat aactataacc cgggggagtt tggtaacggc gggagtaaac
     4621 ccggtggtaa tggcggtaac agcggcaatc atagcgggag ctctggtggc ggacagtctt
     4681 cggccaccgc gatggccttc ggtctgcctg ccctggccac tccgggggct gaaggactgg
     4741 ctttatccgt ttccggcgat gcgttgtcgg ccgctgttgc tgatgtgctg gctgccctga
     4801 aaggtccgtt taagtttggt ctgtggggga ttgcgattta cggagtgctg ccttctgaga
     4861 tagcaaaaga tgatccgaaa atgatgtcaa aaatcgtgac gtcattgccg gcagacacgg
     4921 taacggagac gccggtcagc tccctgccgc tggaccaggc gacggtcagc gtcactaaac
     4981 gtgtggcgga tattgtgaag gacgagcggc agcatattgc ggttgtcacc ggccggccaa
     5041 tgagtgttcc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga
     5101 ttccgggtct cccgtctctg caggtgagcg tacctaaagg tgttccggcc gcgaaagccc
     5161 cgccaaaagg cattattgct gaaaaaggtg attcacgtcc ggctggtttt acggccggtg
     5221 gtaactcccg tgaggccgtt attcgtttcc cgaaagagag cggacagaaa ccggtctatg
     5281 tgtcggtgac ggatgttctt actccggcac aggtaaaaca gcgtctggag gaagaaaagc
     5341 gtcgccagca ggcatgggac gctgctcacc cggaagaggg gctgaaaaga gagtatgata
     5401 aagcgaaagc tgagctggat gctgaagata aaaatattgc gaccttaaac agtcgcattg
     5461 catcgacaga gaaggcgatc cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg
     5521 taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg
     5581 gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatgaga
     5641 aattaaaagc tgcccagaca tctttaaacg aaatgaatga atccttatcc agggataagg
     5701 ctgcgctttc cggggcgatg gagagccgga aacaaaagga gaaaaaagcg aaggatgcag
     5761 aaaataaatt aaatgaggaa aagaaaaaac ctcgtaaggg aactaaagat tacggccatg
     5821 attattttcc tgatcccaag actgaagata ttaaggggtt gggagagttg aaagagggta
     5881 aacctaaaac ccctaaacaa ggtggtggtg gtaagcgggc ccgatggtat ggtgataaaa
     5941 agcgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtac cgcgccagtg
     6001 atggcgaaca cctcggggca ttcgatccaa aaacgggtaa gcaggttaaa gggccggatc
     6061 caaaacgaaa cattaaaaaa tatctttaag aggtaaatat ggggcttaaa ttacatattc
     6121 attggtttga taagaaaacc gaagagttta aaggtggtga atactcaaaa gacttcggtg
     6181 atgatggttc tgtcattgaa agtctgggga tgcctttaaa ggataatatt aataatggtt
     6241 ggtttgatgt tgaaaaatca tgggtttcga tattacagcc acactttaaa aatgtaatcg
     6301 atattagtaa atttgattac tttgtatcct ttgtttaccg ggatggtaac tggtaagcaa
     6361 aagttatcaa ggatgatttg aaatacagac ttccggtgtt cagggatgaa taccggggtt
     6421 tttaaataca gggaaggtat atgaaaaagg taaaggttgt ttttcttttt atattgattt
     6481 tttcaggctt tcttctggtc gcatgtcagg caaactatat ccgggatgtt cagggtggaa
     6541 cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     6601 aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac
     6661 cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta
     6721 actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg
     6781 cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc
     6841 aatggctgct gccagtggcg ttttgcgtgc ttttccgggt tggactcaag tgaacagtta
     6901 ccggataagg cgcagcagtc gggctgaacg gggggttctt gcttacagcc cagcttggag
     6961 cgaacgacct acaccgagcc gagataccag agtgtgagct atgagaaagc gccacacttc
     7021 ccgcaaggga gaaaggcgga acaggtatcc gggaaacggc agggttggaa caggagagcg
     7081 caagagggag cgattcatcg gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac
     7141 tcctgactga ttcatggttg agccaccggc tcccacagat gcaccgaaaa agcgtctgtt
     7201 tatgtgaact cagtcaggag ggcggagcct atggaaaaac gccacctgcg tggccttgcc
     7261 gttttgcctc acatgttagt cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt
     7321 gtccgcagcg cccgccgcag tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc
     7381 gcaatttccc cggcacctac aagacggaca ccggacgcgc tgatgtggca tattgtcccc
     7441 tgattaagtc tttatattcc ctgataataa attaccagga taggtgaatc atatcggttt
     7501 ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttac ggagaaatac
     7561 aggtcatccc cgcgagggcg tggctgacct cagggggata tcccctgctt acgctggctg
     7621 gacgccaccg acgcacggtc tctccccctc tttctggctc cgtccggctg gacgccggcc
     7681 acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg
     7741 gtgctggacg cacctggcga agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc
     7801 tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc
     7861 cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca
     7921 tggggcggaa agaggctggc ctccgtggtg aaagctgaac gcaatcacca cggacgtgac
     7981 cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc
     8041 cggcaaacca tcgtctgaag gagagagaac atgtttaata cggattcgct tgcctggaag
     8101 tggcacattc cggagctggc ccggcagatt tatccgtggg tcagctggct gtaccaggag
     8161 tattacggtc tggccggggc tttcctagtt atcggcctgt ttttcggtct ggctgaactt
     8221 tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga gtatgccgat
     8281 cgctataagc gcgataataa acggccagcg ccattttcgg tgctggcctc gctggattcg
     8341 cccgggcgga tggtcagggc acgtcgacat gtctggaact acgcgtactg gctgaccggc
     8401 gcggccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga
     8461 gaaaccgtcc tgcgtccgga cctctacgca aacgcagatt attacctgct gcagccggct
     8521 aacctgttcg ggattgcctg gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg
     8581 ctgctggcgt tcctgctgct tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg
     8641 aaactgaatc aggagagccg gcgcagcagc cagcggacag gagagatgac cgacgtgcgc
     8701 cacctgcatt tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag
     8761 gcccggcagg agcaggcggt gtttctgggt aaggatgagc agggcaagcc ggtgctggtt
     8821 ccgcgtgata cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg gagcggcaag
     8881 agcgtgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg
     8941 tacttcgacc ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac
     9001 ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc
     9061 gaccagtacg cgctgaaaaa cctgctgatt gccgggctta acctgggcga gaccggcgac
     9121 gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc ggagcagttt
     9181 ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg
     9241 aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg tcgctgacct gagc
//
