LOCUS       CP052149                3991 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F16KP0108 plasmid pF16KP0108-2,
            complete sequence.
ACCESSION   CP052149
VERSION     CP052149.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609967
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:30:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,449
            Genes (coding)                    :: 5,346
            CDSs (with protein)               :: 5,346
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 49 of 103
            Pseudo Genes (incomplete)         :: 50 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 10 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F16KP0108"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF16KP0108-2"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(3862..3991,1..143))
                     /locus_tag="HJX79_27845"
     CDS             complement(join(3862..3991,1..143))
                     /locus_tag="HJX79_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN79274.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(947..1369)
                     /locus_tag="HJX79_27850"
     CDS             complement(947..1369)
                     /locus_tag="HJX79_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJN79271.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1369..1560)
                     /locus_tag="HJX79_27855"
     CDS             complement(1369..1560)
                     /locus_tag="HJX79_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJN79272.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1517..1926
                     /locus_tag="HJX79_27860"
                     /pseudo=""
     CDS             1517..1926
                     /locus_tag="HJX79_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            complement(2307..3281)
                     /locus_tag="HJX79_27865"
     CDS             complement(2307..3281)
                     /locus_tag="HJX79_27865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN79273.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     Typing          1609..2080
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 acctgtattt tacctttccg gtcataccca aaaacggtgt aggtcacatc atcctcatca
       61 tagacttcat gacagatgga gcatgagtcc gggtattttt tgatgagtgc attaatttct
      121 tttacctgag cgtcagtagc caaaacatct ccctgagtca ctgattatca caatccgctg
      181 actctaacac agactcaggc atccgccgtt gatttaccat aacgtcccat tacacgcacc
      241 gctaaaacgg cctcagaacg attctggggc gtaacccatt aaatcctacg tcttaccccc
      301 tgaaaacgcc tcaaaatcgc tccagggcgt tttacgggca tgaatcgtta tgcacttgac
      361 tgactgtctg gggatttcag cctgaattca ggccaggcaa ctcaaaaccc gcaccaaacc
      421 agactgaaaa cggtgaacgg gtgcgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc
      481 agccggaaaa ccggttttca gcacgttcag gaatcgggtt tgtcgagacc agcaatcaca
      541 ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgac tcgtgacttg
      601 actctagagt cgccatggtc aaaaagccac caacccggca agtcgactct agagtcaagt
      661 catcataacg tactgattat atggcgtgat tcacatatga atcgtgggca agtcatcggc
      721 gattcgccat agagtcgtcc acgactcgga ttcaccttac aaaaaataaa attgcgtaat
      781 ttaaaataga taactgattg aaaataaagg tctatctgca aaattttaat tttgcgtagt
      841 gtgtgggtat ttttagtggt gaggagcgtt cagggatctg acgatttttg ggaaggttca
      901 ggaactcccc ggaatggccg gagagcaaaa tggaagcatg gcgggatcag gattttttcc
      961 agaatttcca ccacggggaa tggggtggtg aagaaggttc gtgcttgtgc tccagaagct
     1021 gcatagcctg acgaagggaa tcaatatgct catctcgagc cttaacagcc cctttaagac
     1081 catcgttttc agcttcaagt aaagcgattc tatgttgtaa atcatgtaaa ccaacgctgt
     1141 taatacctgt agattctgcg tcacttttta catcatattt tacagtatcg attacaccat
     1201 caagagaacc aaacactcta attagctctg aagtatcaat aactttaata gctttcccgt
     1261 gctttaccac agaggtgtaa cttaacttcc cactgttaat catcttgtaa attgtggggc
     1321 gactaacctt aacaagccgt gccgcttcac taatagacac tatggccatc agttaccatc
     1381 ctgtaattca tccagtctcg aggacagtgt acggaaaaga tgctccgcat catcatggag
     1441 tttctcacaa aggtcggcct cgacatcgag atcaagctcg ttaagcttat ccagcaggag
     1501 cagagactga ttctggatga atttagccat gttcagcgct gttttctgtt gttttgtcat
     1561 aagaagcctc agagcgtcag tgctcaatgt caaactagtc tcttttccgc ttcctcgctc
     1621 actcagtcgc tacgctccag ccgttcggct gcggcgagcg gcaggagcgt gtacataaaa
     1681 acgtaggcaa ccaagatatg aggcagaaaa gcaaaggccg ccgaacggct gtttttccat
     1741 aggctccgcc cccctgacaa gcatcgcgga aacggacgct caaatcagtg gtggcgaaac
     1801 ccgacaggac ttaaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg
     1861 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttt ctcattccat
     1921 gcctgatact cggctccggg aaggcagttc gctccaagct ggactgtatg cacgaacccc
     1981 ccgttcagtc cgactgctgc gcccgttcca gtaactatct cttgagtcca acccggaaag
     2041 acacgactta tcgccattgg cagcagccac tggtaattga tttagaggag ttagtcttga
     2101 agttatgcgc cagttaaggc taaactaaac gaactgtttt ggtgactgcg ctcctctaag
     2161 ccagttacct cggttcagaa acttgccggt tactgaacct tcgaaaaacc accttgccgg
     2221 gtggtttttt cgtttgaggg gtagagtacg ctgcacctca aaggatcgtc aaggatcttt
     2281 ttggaattca tagagctagc cactgcctat ttgatagcca cctttaattc ctccagctca
     2341 gcgtcagtaa acaacccttc aagatcatta tctttatttc ctatgacctt ggttcttatt
     2401 ttttcttgcc tgcgaatgaa ttcaggatct gtaactttaa tagtcagagc cctgcgaatc
     2461 atatccttaa tatcggagtt atcagcttgg caacgacgca aaatatattg atagcaagcc
     2521 ttattgtaaa taagtatagg gttatcctca ttactcataa gtaactcatc cgtaattgac
     2581 agagcatcat caatacgatc aagtcttttc aaaatataag ctttaagcac taagagcttg
     2641 tcgtagtcat ccttagctac atcctctctt ttaacaagag acaatgattc cgttacagca
     2701 tctaatgcct cagttagttt tttcttaaca gaaagagcct catccggaat ggacatcatt
     2761 gattctgctt tatccacagc tgaaatggct ttaaagtatc ctagctcgaa ttttgttgtt
     2821 ttcttatcca tttcaagctg ttctatcagc tccttgctcc tatcaagctc atctttaaac
     2881 tcatcttgtt cagcctccaa atcttgttgc tttttatcta taccgtctac ttgctctttt
     2941 aatactttgt tggcaagacc atccagtaat gcgtacccaa gatacgcaga gatccccgaa
     3001 acagaaataa gaagcaatat ttgttctatg aacttagttc caccaccatc agccacagga
     3061 aatattatac ttgagtaatc aacatgaaga aatgatgata tgaaaggaac acaaataaat
     3121 gcggatgcta taccaaatct ataaacacta gctcttttat tattctcatc cttatctttt
     3181 gaaaatttat tttgggagtc tcggcaacct ctggaatagc tccccataac accaccaaaa
     3241 caaattagag atgcaacaaa caataggtta aaaaaagcca tgagagaatc cttgcgcaaa
     3301 gcaaacaata aaactttgtt caggtaagtt aagataagct caatcatata ctaaccaata
     3361 acaaaaaagg cagccttggc tgcctttttc tgctgacttg acacttacaa gtctggacgc
     3421 tcatcgtgaa gtttgatttt tttcatagag cacctctcat taattgttcc tttgcaagcc
     3481 catgcttgct tagcgctgaa tatagggcat aaacccagca ctcacaacaa caaaaatgct
     3541 taaaatgata aaatcaaccc atgtattgac attgggtgaa aaaaaaagta taatgcactg
     3601 gtagccaaag gggcggggct tcgcttattc cttaggaata agcgaagccc cgcccctttg
     3661 gctaccataa ttaaatgatt gaatttaaag gattttttta caataacatc cttcctctca
     3721 atagtcatat aaacatgccc catgtattcg tcattctgga tttttcaaaa gaatgaatgc
     3781 gtgcatttac cataacgcct catttcacgc agatatgaaa aagcccgcac aaggcgggct
     3841 tcagactcaa cagatacgga atcactctgt gaaaaactgc tttgccatgg ggtgattctg
     3901 catgatctcg tcacgttctt ccgggtcaaa gcacccacag acacccagca gaaccggctc
     3961 tgtcagcatt ccggcacagc atcccgtggt g
//
