LOCUS       CP052146                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F17KP0012 plasmid pF17KP0012-3,
            complete sequence.
ACCESSION   CP052146
VERSION     CP052146.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609969
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:16:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,381
            Genes (coding)                    :: 5,229
            CDSs (with protein)               :: 5,229
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 78 of 152
            Pseudo Genes (incomplete)         :: 74 of 152
            Pseudo Genes (internal stop)      :: 30 of 152
            Pseudo Genes (multiple problems)  :: 28 of 152
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F17KP0012"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF17KP0012-3"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(20..382)
                     /locus_tag="HJX81_27485"
     CDS             complement(20..382)
                     /locus_tag="HJX81_27485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJN73918.1"
                     /translation="MRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSELIRVY
                     GELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEHKPETA
                     ETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            466..645
                     /locus_tag="HJX81_27490"
     CDS             466..645
                     /locus_tag="HJX81_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJN73919.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(673..1203)
                     /locus_tag="HJX81_27495"
     CDS             complement(673..1203)
                     /locus_tag="HJX81_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN73920.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1210..1941)
                     /locus_tag="HJX81_27500"
     CDS             complement(1210..1941)
                     /locus_tag="HJX81_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJN73921.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(1941..3905)
                     /locus_tag="HJX81_27505"
     CDS             complement(1941..3905)
                     /locus_tag="HJX81_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJN73922.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5386..5535)
                     /locus_tag="HJX81_27510"
     CDS             complement(5386..5535)
                     /locus_tag="HJX81_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJN73923.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5620..5877)
                     /locus_tag="HJX81_27515"
     CDS             complement(5620..5877)
                     /locus_tag="HJX81_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJN73924.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(5887..7572)
                     /locus_tag="HJX81_27520"
     CDS             complement(5887..7572)
                     /locus_tag="HJX81_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJN73925.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            7975..8394
                     /gene="exc2"
                     /locus_tag="HJX81_27525"
     CDS             7975..8394
                     /gene="exc2"
                     /locus_tag="HJX81_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJN73926.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            8700..8963
                     /locus_tag="HJX81_27530"
     CDS             8700..8963
                     /locus_tag="HJX81_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJN73927.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            8953..9252
                     /locus_tag="HJX81_27535"
     CDS             8953..9252
                     /locus_tag="HJX81_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJN73928.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             887..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4705..5283
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1214..1941)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gttggacaag cacagcgcgt cagcgttttc cccacggcca ccaccggcgc ggtgttccgg
       61 ccggggtctc tgctgtctcc ggtttgtgtt ccagcagtct catggactgc atttcctgcc
      121 gtagcccggc cacctcctcc ctgagtgctt ttatctccgc gatcaacgcg tcggaggcca
      181 ttttttcagt ctcacccgac agtctcaact cgtcccgctc gtgagtctca tcctgtctta
      241 actcaccgta cacccggata agctcgctgg tatccaccac ccggccgcca tcagcttccg
      301 tacggtatga tacgcggcct ttcgccatat cccgatacag ggaacttcgg gctttacccg
      361 tcagttccgt cgcctgtctc accgtcaccc acgccattgt ctcaccccgc tatttttgag
      421 acagtatacc tgacaggcgc acaccatgcc cggaaggaaa gaatcatgac aaaacaggaa
      481 aaaacagccc tcaatatggc taaatttatt aaggagcagt cgctgctatt gctcgataaa
      541 ctgaatgagc tggatatgga taccccggcc gacatgtgcg agcggctaca cgaggatgcg
      601 gaaacactct ggcaggtgat gcgggataag ctcggggagg aataacagca ccggcatggc
      661 cggtgctggc atttattttt tgccgaacgg ccaccaccgg cgggcaggtt tgtcctccgg
      721 cagttcaggt accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt
      781 cagcttctcg ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa tcatcgcgtt
      841 aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa
      901 ccgctccacc ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt tgttccgttc
      961 ccattgcgga tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc
     1021 gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa
     1081 aacatacgcc ccggtctgcc aggcgagcac caccatatct tccagatgct cgctggtcgg
     1141 catgatgcag cgctcgccgt tccggtcctc cgcgtcttca ataaatacca tgtacagcgc
     1201 cataatccct cactccgttg tcataaattt aaagcacgca aacaaccgtt cacgatccag
     1261 ctctgccgtt ttgcgggcct cgccggtctg tgggttcacg atttcatcca ccgacaggat
     1321 tgcccgctgc accctgacct gcgtcgcccg gtccgggcag atgtaccaga tttcatcgat
     1381 gccgttagct ttgcggttga acagataccc ggacaccact tcctgatacc ggcgacggct
     1441 cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctccg ccagggcgtc
     1501 cggcactttc atgcctttaa gactcagacg gtgcagaatg cgccagccgg tggcccctct
     1561 ggcttccata tcaaggcgca gggattgcac ggccagccgg tgcggcagat gtgctgccga
     1621 cacccttgac ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt cgaacgagaa
     1681 cgccacgcca tgcggcgtca gcccccacac gctgacctga cggccgtcca ccggatattt
     1741 ggtccggacc agcagccctt tcgccaccag gctgtcgagg aagcggtaga ccggctgcac
     1801 cgccgcaatc cccagccgcc gcgcaaccag ctccgccgtg gaacacagct cttcccgcaa
     1861 ccagcgcagg gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg cgtggctgac
     1921 gttgtacagc tccagcgcca tcagtaaatc cccgcaaaat catcatccgc tttcggcccg
     1981 ccgccgtcgg ccgggggccg gaactccgcc aggggatcgg ttttcgccag caccgggaaa
     2041 cgctgcaacg ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc catccgggcc
     2101 acacccagcc cggtcatgac cgcgaacccg tccggcaggt gttgcagggt gttcacgtca
     2161 aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc cacgttcccc
     2221 gcctcccggc tggcacggcg gcgctccacg tccaccctga tttccccggt ctgccccgcc
     2281 gcccactgcg cggactccag gctggccgcc cggtaaaacc agcggatcgg cgtgttatcc
     2341 agtaccgtgg ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc gaaatcgccc
     2401 agcgactggt gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc cagtgcgttc
     2461 agtacatact tcgacagcag atatttaatt tcatcgagca tcacgctgac gtgtggccac
     2521 tcccggaact cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag cattttttgc
     2581 acacggatca ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc gccgccgttg
     2641 ataatccccg ccacatcgat gccactgtcc gtctgcagga cgctcaggtc agcgacgttt
     2701 tccagcctag tgatcaaccc cttcacgtcc tttttcaggg cctccggcag cgcgtacgcc
     2761 gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat cagcttcgcc
     2821 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc caggttaagc
     2881 ccggcaatca gcaggttttt cagcgcgtac tggtcgagat cgcggaacag attcagctgc
     2941 gccggtttcc ccggccgcag atccagcagg gtgaagtccg gacagtgcgc ccggaagacg
     3001 tgcggcgccc atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc gaaatcgcgt
     3061 acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg caggccgagg
     3121 atctgaatgt tggtcttgcg ccaggtatca cgcggaacca gcaccggctt gccctgctca
     3181 tccttaccca gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa atcggccagc
     3241 gcattaaccg gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat ctctcctgtc
     3301 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcagcc
     3361 gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat cccgacagcc
     3421 agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag gtaataatct
     3481 gcgtttgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat aaacacggcg
     3541 acaaccagcc cccccagaag ggccagaccg gccgcgccgg tcagccagta cgcgtagttc
     3601 cagacatgtc gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc cagcaccgaa
     3661 aatggcgctg gccgtttatt atcgcgctta tagcgatcgg catactcccg tcgctcctta
     3721 tgactcagcc ccacaaaacg catccgtccg gcccaaagtt cagccagacc gaaaaacagg
     3781 ccgataacta ggaaagcccc ggccagaccg taatactcct ggtacagcca gctgacccac
     3841 ggataaatct gccgggccag ctccggaatg tgccacttcc aggcaagcga atccgtatta
     3901 aacatgttct ctctccttca gacgatggtt tgccggcgtg atgtgcagcc cccgctgcac
     3961 aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca
     4021 gctttcacca cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg
     4081 aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc
     4141 ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg
     4201 agcagctttg ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc
     4261 gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacggagcc
     4321 agaaagaggg ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca ggggatatcc
     4381 ccctgaggtc agccacgccc tcgcggggat gacctgtatt tctccgtaag gagaaaaaag
     4441 caacagagat aggttaacgt aaaatgttca gcgaaaaacc gatatgattc acctatcctg
     4501 gtaatttatt atcagggaat ataaagactt aatcagggga caatatgcca catcagcgcg
     4561 tccggtgtcc gtcttgtagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac
     4621 gctccggccg tgagactgcg gcgggcgctg cggacacatg caaagttacc cacagattcc
     4681 gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag gtggcgtttt
     4741 tccataggct ccgccctcct gactgagttc acataaacag acgctttttc ggtgcatctg
     4801 tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac aggacttaaa
     4861 gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca accctgccgt
     4921 ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt ctcatagctc
     4981 acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     5041 ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     5101 gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     5161 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     5221 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
     5281 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     5341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     5401 ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat cccggatata
     5461 gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa gaaaaacaac
     5521 ctttaccttt ttcatatacc ttccctgtat ttaaaaaccc cggtattcat ccctgaacac
     5581 cggaagtctg tatttcaaat catccttgat aacttttgct taccagttac catcccggta
     5641 aacaaaggat acaaagtaat caaatttact aatatcgatt acatttttaa agtgtggctg
     5701 taatatcgaa acccatgatt tttcaacatc aaaccaacca ttattaatat tatcctttaa
     5761 aggcatcccc agactttcaa tgacagaacc atcatcaccg aagtcttttg agtattcacc
     5821 acctttaaac tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa gccccatatt
     5881 tacctcttaa agatattttt taatgtttcg ttttggatcc ggccctttaa cctgcttacc
     5941 cgtttttgga tcgaatgccc cgaggtgttc gccatcactg gcgcggtacc cttcaagctc
     6001 accgtgctgg gaatcccatt cataaatttt acgcttttta tcaccatacc atcgggcccg
     6061 cttaccacca ccaccttgtt taggggtttt aggtttaccc tctttcaact ctcccaaccc
     6121 cttaatatct tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt tagttccctt
     6181 acgaggtttt ttcttttcct catttaattt attttctgca tccttcgctt ttttctcctt
     6241 ttgtttccgg ctctccatcg ccccggaaag cgcagcctta tccctggata aggattcatt
     6301 catttcgttt aaagatgtct gggcagcttt taatttctca ttctgattct gaatatcctt
     6361 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     6421 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     6481 agcccttgca ccggggatcg ccttctctgt cgatgcaatg cgactgttta aggtcgcaat
     6541 atttttatct tcagcatcca gctcagcttt cgctttatca tactctcttt tcagcccctc
     6601 ttccgggtga gcagcgtccc atgcctgctg gcgacgcttt tcttcctcca gacgctgttt
     6661 tacctgtgcc ggagtaagaa catccgtcac cgacacatag accggtttct gtccgctctc
     6721 tttcgggaaa cgaataacgg cctcacggga gttaccaccg gccgtaaaac cagccggacg
     6781 tgaatcacct ttttcagcaa taatgccttt tggcggggct ttcgcggccg gaacaccttt
     6841 aggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg
     6901 ttttgtcggt ttcgcatcca ccacaggaac actcattggc cggccggtga caaccgcaat
     6961 atgctgccgc tcgtccttca caatatccgc cacacgttta gtgacgctga ccgtcgcctg
     7021 gtccagcggc agggagctga ccggcgtctc cgttaccgtg tctgccggca atgacgtcac
     7081 gatttttgac atcattttcg gatcatcttt tgctatctca gaaggcagca ctccgtaaat
     7141 cgcaatcccc cacagaccaa acttaaacgg acctttcagg gcagccagca catcagcaac
     7201 agcggccgac aacgcatcgc cggaaacgga taaagccagt ccttcagccc ccggagtggc
     7261 cagggcaggc agaccgaagg ccatcgcggt ggccgaagac tgtccgccac cagagctccc
     7321 gctatgattg ccgctgttac cgccattacc accgggttta ctcccgccgt taccaaactc
     7381 ccccgggtta tagttatgaa tatccccgtt aggtgtatgc gtcgtacccc agcccggccc
     7441 gctgtttgga tccgtaccac cacctgatga agggccacct ttacctgatg taccgttcac
     7501 attcccactg gcctgaccac cattgtgtcc cagacctgaa ttacccggac ctcgtccgtc
     7561 tccgccactc atatactttt cctctttgac ttttaaaaca ataaactaaa ataaaatact
     7621 gtacatataa ccactggttt tatatacagt ataaaaatta cgctgccttg tttttcatgt
     7681 caagtctgta aatttgcgtt tttgtgaaaa tgatcaaaaa aacagccact cacaccatgc
     7741 gcaatcccct ccagatgcaa caaaaaccag cacaaacggc gccgttctcc ggcctcagta
     7801 tgttttactg aaatcattat atgagatacc agaaagcccc ctccgggaca ggaaaagaaa
     7861 cggggggtct ggttcgggtt gttcggtcac tgaacacggt cagaaactga tgataagata
     7921 gcctgcggat atataactca atgagaaaaa agaattaaag gtaaggagac gctcatgaga
     7981 cgttccggca gcaaactgtg tcttatcgcc atgataacag cgacagcaac tctcactgcc
     8041 tgtgctccaa agggcagcgt ggaacagcat acccggcatt acgtatatgc ttcagatgac
     8101 ggttttgatc ccaatttttc cacccagaaa gctgacacaa cacgaatgat ggtgcctttt
     8161 ttccggcagt tctgggatat gggcgcaaag gataaagcga ccggaaaatc acggagtgat
     8221 gtgcagcaac gcattcagca gtttcacagt caggaatttt taaactcact ccaaggcaca
     8281 acacaatttg cgggtaccga ttaccgcagc aaagacctca caccgaaaaa atcaaggcta
     8341 ctggctgaca ccatttcagc tgtttatctc gatggctatg aaggcagaca gtaagtggat
     8401 ttaccataac gcgtcattac gcgcaccgct cagacgggct cagggcgttt ccggcggcta
     8461 cgggataaaa caggcaacaa cccccccgga aaactgccgg aaacgcgccc gataaatttt
     8521 atcctaatgc atgcctatgc acgcaactgc atggcatatg ttttgacgta aaatggatgc
     8581 cgtaagccct tttttaccga ctttctgctt catcagcctg actgacacga ccatattctt
     8641 tcagaccgcc ccctgctaaa cttccttcat attgccgcat taacccggga ggctccgcaa
     8701 tgagcaacac tacacactac gaaaacgcca actttcttcg ggaactggct gaaagcctgc
     8761 cccgcatact gccggaaggt ggcccggaca aagctgcgct gttgcagcga ctggctaacg
     8821 aagaactggc ccaggcagag tatgaagatc aggttcgcgc caaagtgacc gccgcccgtg
     8881 ctgacacgcg tccgggtatg accacggaac aactccgcca gcgcctgcat ggccgttatc
     8941 aggaactgcg cgatgccgta tgacgtggaa tggaagcagg gagccatcga ggatgtaacg
     9001 acgctgtttg actatatcgc ggaaaattca tccctgtggg atgcgcagaa cgttaccgac
     9061 cgggttctcg ctgcggcgga caaactggcc gacttcccgc gactctatga agtggactca
     9121 cgctatggcg aggacgttcg ccggatcagt ctgatgggac agcatgtcct ctatgatgtt
     9181 gatgaccaga ccagaaaagt ccgggttctg gccgtggtcg gccagcgcca gaatccccat
     9241 attgttctct aataaccggt gacggggacg caattcattg cgtggccgtc gccg
//
