LOCUS       CP052142                3896 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F17KP0040 plasmid pF17KP0040-4,
            complete sequence.
ACCESSION   CP052142
VERSION     CP052142.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609970
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:06:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,741
            CDSs (total)                      :: 5,620
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 58 of 131
            Pseudo Genes (incomplete)         :: 69 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 12 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F17KP0040"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF17KP0040-4"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(3729..3896,1..807)
                     /locus_tag="HJX82_28685"
     CDS             join(3729..3896,1..807)
                     /locus_tag="HJX82_28685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN58094.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(1188..1597)
                     /locus_tag="HJX82_28690"
                     /pseudo=""
     CDS             complement(1188..1597)
                     /locus_tag="HJX82_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            1554..1745
                     /locus_tag="HJX82_28695"
     CDS             1554..1745
                     /locus_tag="HJX82_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJN58091.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1745..2167
                     /locus_tag="HJX82_28700"
     CDS             1745..2167
                     /locus_tag="HJX82_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJN58092.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2879..3151
                     /locus_tag="HJX82_28705"
     CDS             2879..3151
                     /locus_tag="HJX82_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN58093.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          complement(1035..1506)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 tgtgttcctt tcatatcatc atttcttcat gttgattact caagtataat atttcctgtg
       61 gctgatggtg gtggaactaa gttcatagaa caaatattgc ttcttatttc tgtttcgggg
      121 atctctgcgt atcttgggta cgcattactg gatggtcttg ccaacaaagt attaaaagag
      181 caagtagacg gtatagataa aaagcaacaa gatttggagg ctgaacaaga tgagtttaaa
      241 gatgagcttg ataggagcaa ggagctgata gaacagcttg aaatggataa gaaaacaaca
      301 aaattcgagc taggatactt taaagccatt tcagctgtgg ataaagcaga atcaatgatg
      361 tccattccgg atgaggctct ttctgttaag aaaaaactaa ctgaggcatt agatgctgta
      421 acggaatcat tgtctcttgt taaaagagag gatgtagcta aggatgacta cgacaagctc
      481 ttagtgctta aagcttatat tttgaaaaga cttgatcgta ttgatgatgc tctgtcaatt
      541 acggatgagt tacttatgag taatgaggat aaccctatac ttatttacaa taaggcttgc
      601 tatcaatata ttttgcgtcg ttgccaagct gataactccg atattaagga tatgattcgc
      661 agggctctga ctattaaagt tacagatcct gaattcattc gcaggcaaga aaaaataaga
      721 accaaggtca taggaaataa agataatgat cttgaagggt tgtttactga cgctgagctg
      781 gaggaattaa aggtggctat caaataggca gtggctagct ctatgaattc caaaaagatc
      841 cttgacgatc ctttgaggtg cagcgtactc tacccctcaa acgaaaaaac cacccggcaa
      901 ggtggttttt cgaaggttca gtaaccggca agtttctgaa ccgaggtaac tggcttagag
      961 gagcgcagtc accaaaacag ttcgtttagt ttagccttaa ctggcgcata acttcaagac
     1021 taactcctct aaatcaatta ccagtggctg ctgccaatgg cgataagtcg tgtctttccg
     1081 ggttggactc aagagatagt tactggaacg ggcgcagcag tcggactgaa cggggggttc
     1141 gtgcatacag tccagcttgg agcgaactgc cttcccggag ccgagtatca ggcatggaat
     1201 gagaaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1261 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc
     1321 caccactgat ttgagcgtcc gtttccgcga tgcttgtcag gggggcggag cctatggaaa
     1381 aacagccgtt cggcggcctt tgcttttctg cctcatatct tggttgccta cgtttttatg
     1441 tacacgctcc tgccgctcgc cgcagccgaa cggctggagc gtagcgactg agtgagcgag
     1501 gaagcggaaa agagactagt ttgacattga gcactgacgc tctgaggctt cttatgacaa
     1561 aacaacagaa aacagcgctg aacatggcta aattcatcca gaatcagtct ctgctcctgc
     1621 tggataagct taacgagctt gatctcgatg tcgaggccga cctttgtgag aaactccatg
     1681 atgatgcgga gcatcttttc cgtacactgt cctcgagact ggatgaatta caggatggta
     1741 actgatggcc atagtgtcta ttagtgaagc ggcacggctt gttaaggtta gtcgccccac
     1801 aatttacaag atgattaaca gtgggaagtt aagttacacc tctgtggtaa agcacgggaa
     1861 agctattaaa gttattgata cttcagagct aattagagtg tttggttctc ttgatggtgt
     1921 aatcgatact gtaaaatatg atgtaaaaag tgacgcagaa tctacaggta ttaacagcgt
     1981 tggtttacat gatttacaac atagaatcgc tttacttgaa gctgaaaacg atggtcttaa
     2041 aggggctgtt aaggctcgag atgagcatat tgattccctt cgtcaggcta tgcagcttct
     2101 ggagcacaag cacgaacctt cttcaccacc ccattccccg tggtggaaat tctggaaaaa
     2161 atcctgatcc cgccatgctt ccattttgct ctccggccat tccggggagt tcctgaacct
     2221 tcccaaaaat cgtcagatcc ctgaacgctc ctcaccacta aaaataccca cacactacgc
     2281 aaaattaaaa ttttgcagat agacctttat tttcaatcag ttatctattt taaattacgc
     2341 aattttattt tttgtaaggt gaatccgagt cgtggacgac tctatggcga atcgccgatg
     2401 acttgcccac gattcatatg tgaatcacgc catataatca gtacgttatg atgacttgac
     2461 tctagagtcg acttgccata atgtgattgc tggtctcgac aaacccgatt cctgaacgtg
     2521 ctgaaaaccg gttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgca
     2581 cccgttcacc gttttcagtc tggtttggtg cgggttttga gttgcctggc ctgaattcag
     2641 gctgaaatcc ccagacagtc agtcaagtgc ataacgattc atgcccgtaa aacgccctgg
     2701 agcgattttg aggcgttttc agggggtaag acgtaggatt taatgggtta cgccccagaa
     2761 tcgttctgag gccgttttag cggtgcgtgt aatgggacgt tatggtaaat caacggcgga
     2821 tgcctgagtc tgtgttagag tcagcggatt gtgataatca gtgactcagg gagatgtttt
     2881 ggctactgac gctcaggtaa aagaaattaa tgcactcatc aaaaaatacc cggactcatg
     2941 ctccatctgt catgaagtct atgatgagga tgatgtgacc tacaccgttt ttgggtatga
     3001 ccggaaaggt aaaatacagg tcaccacggg atgctgtgcc ggaatgctga cagagccggt
     3061 tctgctgggt gtctgtgggt gctttgaccc ggaagaacgt gacgagatca tgcagaatca
     3121 ccccatggca aagcagtttt tcacagagtg attccgtatc tgttgagtct gaagcccgcc
     3181 ttgtgcgggc tttttcatat ctgcgtgaaa tgaggcgtta tggtaaatgc acgcattcat
     3241 tcttttgaaa aatccagaat gacgaataca tggggcatgt ttatatgact attgagagga
     3301 aggatgttat tgtaaaaaaa tcctttaaat tcaatcattt aattatggta gccaaagggg
     3361 cggggcttcg cttattctaa ggaataagcg aagccccgcc cctttggcta ccagtgcatt
     3421 atactttttt tcacccaatg tcaatacatg ggttgatttt atcattttaa gcatttttgt
     3481 tgttgtgagt gctgggttta tgccctatat tcagcgctaa gcaagcatgg gcttgcaaag
     3541 gaacaattaa tgagaggtgc tctatgaaaa aaatcaaact tcacgatgag cgtccagact
     3601 tgtaagtgtc aagtcagcag aaaaaggcag ccaaggctgc cttttttgtt attggttagt
     3661 atatgattga gcttatctta acttacctga acaaagtttt attgtttgct ttgcgcaagg
     3721 attctctcat ggcttttttt aacctattgt ttgttgcatc tctaatttgt tttggtggtg
     3781 ttatggggag ctattccaga ggttgccgag actcccaaaa taaattttca aaagataagg
     3841 atgagaataa taaaagagct agtgtttata gatttggtat agcatccgca tttatt
//
