LOCUS       CP052141                5472 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain F17KP0040 plasmid pF17KP0040-3,
            complete sequence.
ACCESSION   CP052141
VERSION     CP052141.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609970
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:06:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,741
            CDSs (total)                      :: 5,620
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 58 of 131
            Pseudo Genes (incomplete)         :: 69 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 12 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F17KP0040"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF17KP0040-3"
                     /geo_loc_name="South Korea: Busan"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(4575..5472,1..854)
                     /locus_tag="HJX82_28655"
                     /pseudo=""
     CDS             join(4575..5472,1..854)
                     /locus_tag="HJX82_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            896..1138
                     /locus_tag="HJX82_28660"
     CDS             896..1138
                     /locus_tag="HJX82_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="QJN58090.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(1170..1820)
                     /gene="tetR(A)"
                     /locus_tag="HJX82_28665"
     CDS             complement(1170..1820)
                     /gene="tetR(A)"
                     /locus_tag="HJX82_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="QJN58089.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1926..3125
                     /gene="tet(A)"
                     /locus_tag="HJX82_28670"
     ARG             1927..3122
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3157..4041)
                     /locus_tag="HJX82_28675"
     CDS             complement(3157..4041)
                     /locus_tag="HJX82_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="QJN58087.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(4179..4571)
                     /locus_tag="HJX82_28680"
     CDS             complement(4179..4571)
                     /locus_tag="HJX82_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="QJN58088.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="cysteine hydrolase"
ORIGIN
        1 gacggctgtt ctatacccat atctccgacc agtacgcgcc gttcagcacc cgcgtggtga
       61 atgtcggcgt ccgcgattcc acctatgtgc tcgacggcct gctgtaccac gagtccgacc
      121 tgcggatcga ggagcactac accgacacgg ccggcttcac cgatcacgtc tttgccctga
      181 tgcacctgct aggcttccgc ttcgcgccgc gcatccgcga cctcggcgaa accaagctgt
      241 acgtgccgca gggcgtgcaa gcctacccga cgttgcgccc gctgatcggc ggcaccctga
      301 acatcaagca cgtgcgtgcc cactgggacg acatcctgcg cctggccagc tcgatcaagc
      361 agggcaccgt caccgcctcg ctgatgctgc gcaagctcgg cagctacccg cgccagaacg
      421 gactggccgt ggccctgcgc gagctgggcc ggatcgagcg cacgctgttc atcctggact
      481 ggctgcaaag tgttgaactg cgccgccgcg tgcatgccgg cctgaacaaa ggtgaggcgc
      541 gcaactcgct ggccagggcg gtgttcttca accgccttgg ggaaatcagg gatcggagct
      601 tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggct atcgtgctgt
      661 ggaacacggt gtacctggaa cgcgccaccc aggggttggt cgaggccggc aagccggtgg
      721 acggcgagct gctgcaattc ctgtcgccgc tgggctggga gcacatcaac ctaaccggcg
      781 attacgtctg gcggcagagc cgcagactgg aagacgggaa gtttcggccc ttacggatgc
      841 ccggaaaacc ttagcgtacg attttttccg aattctgcgg gctcccctat ctcatctgcg
      901 caaggcagaa cgtgaagacg gccgcgagcg ccaggcgcac gaggccggcg cgcggacccg
      961 cgccacggcc cacgagcgga cgccgcagca ggagcgccag aaggccgcca gagaggccga
     1021 gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta
     1081 cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg
     1141 agtgggttgc gctccggcag cggtcctgat caatcgtcac cctttctcgg tccttcaacg
     1201 ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg
     1261 aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag
     1321 agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc
     1381 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
     1441 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
     1501 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
     1561 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
     1621 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
     1681 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
     1741 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
     1801 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
     1861 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
     1921 cagacgtgaa acccaacaga cccctgatcg taattctgag cactgtcgcg ctcgacgctg
     1981 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
     2041 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttgatg caatttgcct
     2101 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct
     2161 cgctggccgg cgctgctgtc gactacgcca tcatggcgac ggcgcctttc ctttgggttc
     2221 tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt
     2281 atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct
     2341 gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc
     2401 cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt
     2461 tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc
     2521 cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct
     2581 tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg
     2641 atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc
     2701 attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg
     2761 cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac
     2821 ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg
     2881 cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct
     2941 cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct
     3001 atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact
     3061 tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc
     3121 gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat
     3181 acgcgcccta ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag
     3241 gacgccgatg atttgaagcg cactcagcgt ctgatccaag aacaaccatc ctagcaacac
     3301 ggcggtcccc gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     3361 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     3421 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     3481 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     3541 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     3601 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
     3661 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
     3721 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
     3781 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
     3841 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
     3901 taaacccgcc ggcaacgccc gcagcatcgc gaccgtcatc ggtgagaagt tcggcaggta
     3961 ttgggtggtg acaatgtagg tgctgcccca aatggcaggt gctatcgctg tgaacaataa
     4021 atcgggcgtg cgtaatgaca tgcgattaca agacctccgc tacggcgatg ggtttttgag
     4081 agttgcaggc gccgcgatga ccgcaccctg acttggactg gccctgcctc gcaccaatcc
     4141 acctgcggct atggccgcga tcatgcgagg cccgaaaatt acacgacctg tacacgtccg
     4201 ccgaacccgg aacgcaacag agcttcgtgg gcatcccgat ccgggtcgta gcagcagtcc
     4261 tggaccaaac gcacgtcgta gtccgcatca ctagcccagg cgacgcttga aagaacaacg
     4321 ccggtggtgc ttatcccggc catgacaagc gtgcttacgc cgcgcgtccg aaggtcggcg
     4381 tcgagcgccg tgccataaaa gacgctggct cgcggacatg catagaaaag gtcgccccgt
     4441 tcgatcgcaa gcccttcgac gggcagaccg gtgcgaaatc gtccgctcgg gagatacggt
     4501 gagatttggc gattcgtcgc cggcggcgca tgttcatact cttcgccgag cgaaaagttg
     4561 gggaacagca cgggccggcc gcgcaaggcg tgctggcagc cgtgcagacc ctgcgtgaga
     4621 tgaacgccga caacctgcgc aaggtgccgg ccgatgcacc cacggccttc atcaagccgc
     4681 gctggaagcc gctggtgatc accccggaag gcctcgaccg gaaattctac gaaatctgcg
     4741 ccctgtccga gctgaagaac gccctgcgct ccggcgacat ctgggtcaag ggctcgcggc
     4801 agttccgcga cttcgacgac tacctgctgc cggccgagaa gttcgccgca ctcaagcgcg
     4861 agcaggccct gcccctggcg atcaacccga acagcgacca gtacctggaa gagcgtttgc
     4921 agctgctgga cgagcagttg gccaccgtca cccgcctggc caaggacaac gagctgcccg
     4981 atgccatcct caccgagtca gggctgaaaa tcaccccgct ggatgcggcg gtgccggatc
     5041 gggcgcaggc gctgatcgac caaaccagcc agttactgcc gcgcatcaag atcaccgaac
     5101 tgctgatgga cgtggacgac tggacgggct tcagccgcca cttcacccac ttgaaggacg
     5161 gggccgaggc caaagacagg acgttgctgc tgtccgcaat cctcggtgat gcgatcaacc
     5221 tcgggctgac caagatggcc gagtcgagcc ccggcctgac ctacgccaag ctgtcctggc
     5281 tgcaagcctg gcacatccgc gacgaaacct attcggcggc cttggccgag ctggtcaacc
     5341 accagtatcg ccacgccttt gccgcccact ggggcgacgg cacgacctca tcctccgatg
     5401 gccagcgctt ccgcgcgggt ggccggggcg agagcaccgg gcacgtcaac ccgaagtacg
     5461 gtagcgagcc gg
//
