LOCUS       CP051993               13163 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain ER08597.3 plasmid pER08597.3A.2,
            complete sequence.
ACCESSION   CP051993
VERSION     CP051993.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN14669395
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.580385723231x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/23/2020 10:19:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,019
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,660
            CDSs (with protein)               :: 2,660
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 251 of 278
            Pseudo Genes (incomplete)         :: 28 of 278
            Pseudo Genes (internal stop)      :: 13 of 278
            Pseudo Genes (multiple problems)  :: 14 of 278
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER08597.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER08597.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2017"
                     /collected_by="Mount Sinai Hospital"
     gene            1102..1758
                     /locus_tag="HIK55_15025"
     CDS             1102..1758
                     /locus_tag="HIK55_15025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="QOB75851.1"
                     /translation="MKKDKYYIYNEKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRM
                     SWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSY
                     KSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTG
                     IVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNDQ"
                     /name="class D beta-lactamase"
     gene            1748..2128
                     /gene="blaI"
                     /locus_tag="HIK55_15030"
     ARG             1749..2125
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            2392..2970
                     /locus_tag="HIK55_15035"
     CDS             2392..2970
                     /locus_tag="HIK55_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QOB75853.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(3331..4074)
                     /locus_tag="HIK55_15040"
     CDS             complement(3331..4074)
                     /locus_tag="HIK55_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QOB75854.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            4500..5483
                     /locus_tag="HIK55_15045"
     CDS             4500..5483
                     /locus_tag="HIK55_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QOB75855.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            5476..5792
                     /locus_tag="HIK55_15050"
                     /pseudo=""
     CDS             5476..5792
                     /locus_tag="HIK55_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            6339..6488
                     /locus_tag="HIK55_15055"
     CDS             6339..6488
                     /locus_tag="HIK55_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB75856.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(6656..7348)
                     /locus_tag="HIK55_15060"
     CDS             complement(6656..7348)
                     /locus_tag="HIK55_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB75857.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            7458..7652
                     /locus_tag="HIK55_15065"
     CDS             7458..7652
                     /locus_tag="HIK55_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB75858.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            8767..9384
                     /gene="cadD"
                     /locus_tag="HIK55_15070"
     ARG             8768..9381
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9403..9750
                     /locus_tag="HIK55_15075"
     CDS             9403..9750
                     /locus_tag="HIK55_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QOB75860.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            9907..9999
                     /locus_tag="HIK55_15080"
     CDS             9907..9999
                     /locus_tag="HIK55_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QOB75861.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10171..11142)
                     /locus_tag="HIK55_15085"
                     /pseudo=""
     CDS             complement(10171..11142)
                     /locus_tag="HIK55_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /name="oxidoreductase"
     gene            11266..11685
                     /locus_tag="HIK55_15090"
     CDS             11266..11685
                     /locus_tag="HIK55_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QOB75862.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(12211..13056)
                     /gene="blaZ"
                     /locus_tag="HIK55_15095"
     ARG             complement(12215..13056)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(12212..13056)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          5849..5996
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(3332..4074)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4501..5483
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5615..5784
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 tggctaaatt actaataatg agcgtagtaa gcttttgttt tatatttcta ttgttagtat
       61 tttttaggta catattaaaa cgctatttta attatagttt aaattataaa gtttggtatc
      121 taactatgct tgctggatta attcctttta tcccaattaa attctctttt ataaaattta
      181 ataatgtgaa taatcaagcg cccacagttg aaagtaagtc acacgatttg aaccataaca
      241 taaataccac caaacctatt caagagttca caacagatat ccataagttt aattgggatt
      301 caattgataa tatctgcaca gttatttgga tagttttagt tattatttta agttttaaat
      361 ttttgaaagc cttattatat cttaaatatt taaagaaaca gtcactttat ctaaacgaaa
      421 atgaaaaaaa taaaatagat acgatacttt tcaaccatca atataaaaaa aatattgtga
      481 ttcgaaaagc agaggctatt caatctccaa taactttttg gtatgggaaa tatattattt
      541 tgattcctag ttcatatttt aaaagtgtaa ttgataaaag actaaaatat ataatactgc
      601 atgaatatgc tcatgctaaa aatagagata ctttacattt aattatcttt aatatcttct
      661 ctattattat gagttacaat ccattagtac atattgtaaa aagaaagata attcatgaca
      721 atgaagtaga agccgataga tttgtcctta ataatattaa caaaaatgaa tttaaaactt
      781 atgcggaatc tattatggac tccgtattaa aaaccccatt ttccaacaaa aatatattaa
      841 gccattcatt taatggtaaa aaatcattac tcaaaagtag attaattaat ataaaagaag
      901 ccgatctgaa aaagcagtca aaattaattc taatttttat ttgtattttt acttttttta
      961 tcatgataat tcaaagccag tttttaatgg ggcaatcctt aactgattat aattataaaa
     1021 aagcccttac aaagcgatta ccaaatttta gatgaaagta aaaattttgg ttcaaatagt
     1081 ggatctttcg ttatgtacag catgaagaaa gacaaatact atatttataa tgaaaaagaa
     1141 agtagaaaaa gatattctcc tgattcaact tacaaaattt atttagcact gtttggactt
     1201 gaccgtcata ttataagtga taaaaattca cgtatgtcat ggaatcataa acattatctt
     1261 ttcgagtctt ggaataagga acaagattta aatacagcaa tgcaaaattc agttaattgg
     1321 tacttcgaac gtattagtaa tcaaatacca aagaactata ctgcggctca actcaagcaa
     1381 ttaaattatg gtaatgaaaa tttgggaagt tataaaagct attggatgga agatagtttg
     1441 aaaatatcta atcttgaaca agtaatagtt tttaaaaata tgatggaaca aaataaccat
     1501 tttagtaaaa aagcaaagaa tcaattatct tcttcattat tgattaagaa aaatgaaaag
     1561 tatgaactgt atgggaaaac aggtacaggt atagtaaacg ggaagtataa taatgggtgg
     1621 tttgtaggtt acgtaattac aaatcatgat aagtattatt ttgctacaca tttatcagat
     1681 ggaaacccgt ctgggaaaaa tgctgaatta ataagtgaaa aaatattaaa aggaatgggt
     1741 gttttaaatg accaataagc aagttgaaat atctatggct gaatgggatg ttatgaatat
     1801 aatatgggat aaaaaatcag tatcagctaa tgaaattgta gttgaaattc aaaaatataa
     1861 agaagttagt gataaaacga ttagaacatt aatcacaaga ctatataaaa aagagattat
     1921 aaaacgatac aaatcagaaa atatttattt ttactcatca aatattaaag aagacgatat
     1981 taaaatgaaa actgctaaaa cctttcttaa taaactgtat ggaggggata tgaaaagttt
     2041 agtgctgaat tttgcgaaaa atgaagaatt aaataacaaa gaaattgaag aattgcgaga
     2101 cattttaaat gatattagta aaaagtaatt agaggtatgt ttcaaacata atacatatgt
     2161 aaaccatact tgactcttat taaattagtt ttgttaatga catttaaaat atattattaa
     2221 agatgagagt gtccagaaaa taaccatttt ctgtacactc ttgttttttg tctaaataag
     2281 taagtattac atagaattat gtgttcaaaa acccaacaaa aaatctatgt tcacaaaaga
     2341 cctcgtaatc aagttatggt acactaaagg tgtatatagg aggtctgtat tttgaaaata
     2401 ggttatgcaa gagtttcaac tggattacaa aatttgaatt tacaggagga tcgattaaat
     2461 acatatggtt gtgaaaagat atttaatgac catatgagtg gttcaaaaag taagcgacct
     2521 ggtttagata aagcaattga atttgcgagg tcaggagata cgattgttgt ttggagatta
     2581 gatagactag gacggaacat ggaggattta atcacgttag ttaatgaact taacgaacga
     2641 ggtgtgagtt ttcatagttt agaagaaaat ataacgatgg ataaatcaag ttctacagga
     2701 caattattat ttcatttatt cgcagcattt gcagagtttg aacgtaattt aattttagag
     2761 cgatcatcag ccggtcgaat tgcagcacgt gctagaggcc gatatggagg tagacctgaa
     2821 aaattaaata aacaagattt aaacttactt aagacacttt atgataatgg tacgccaatt
     2881 aaaacaattg cagagcagtg gcaagtttca cgtacaacta tttatcgtta tctcaataaa
     2941 ttagaggcaa aagagaataa aaacaagtag agcaactaat ctagcttact ataatgaatc
     3001 tactaaataa ttttatatat cattaacata caaagacttt gattcaatgt cactcgatgt
     3061 aaaagagacg ttctgacagc gtctaaaatc tcataatatt ttcctgttat gagattttaa
     3121 taataacaca agaaaaaagc gtacaaattc gtacgctttt ttaatataat acctgtttca
     3181 ctaatgaacg tttataacgc aatcatgatg agagtattaa tacagttaat tatttgaaat
     3241 actttagttt acataatcga tgattaccag aagttctcac tatataaaag acactaaaat
     3301 tttataactt ttagtgccta atcaattatt ttagctacca aacaaacgac tccaaaaccc
     3361 ctttttaggt tccgtttctt cactcttttc ttctatatca ctatcattgt catttagatt
     3421 agctggaacg tcttcttttt ttgcttcttc accttgtgta ctttcgtcgc tttgacttcc
     3481 agatatatcc ttatattgaa tctctgtttc tttagtttct tcctgatctc tgttaatatt
     3541 tacaggatta gtagtatatg ttttttcttg tgcatcaacg ttttgtctag tgttagtaga
     3601 tgtatcaaag ggataattaa ggtgtttttc tttttctaat tgattttcta atttttgaat
     3661 ctttttatta ctctctaatg ccagaacttg ctgattttca agcaattttg atctttcttg
     3721 caactctttt ctgtaaaatt caattgttgt ttcatgacga ctttcttgtt tttcaatttg
     3781 attatttaaa gtatcaatct gatttttaag gatttctact atttgagcat cgcttttcga
     3841 ttcaaaatta atattatccg accctttttt ttgtttgtct tcagtagtac tttcaaaacc
     3901 atatttcttt ttattttcat ttactctttg aatgattttt tctatatcgg tatcttcttt
     3961 aataaacgaa gtgttttcct gttttattgt ctctatattc aatcgcttaa tattattaaa
     4021 aatcgtttgc ttactaaccc ctaattcttc actcaacttt ttcacacttt tcatatcatc
     4081 aaccctttat aaaatgttta taaacccttg ataaaatatt ataagcttat aatgcttaat
     4141 aataaagtga ttaataaata aaaataaaca tccatcaacc tttttatcaa gggtttatga
     4201 aaggtttatg atatttatca agggtttata aacccttgat aaagctatat tttccctttt
     4261 ataagtttat ttaaagtgct ataataaact taatataaca aaaaccccaa ctactggtaa
     4321 tagtcggggt ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt
     4381 gtattatata atgaataata tcttagtgca agcacctaaa tcattgaata aatgtaaagc
     4441 taaataaaaa cgttttatta cctcttacca aactaaaata tggaaagagg ttttttgtca
     4501 tgcaaaatca atattttaca gtgcaagaaa attacaaaga aagattttat caaatcccta
     4561 aagtattctt taccagtgaa aattataaaa atctaactaa tgatatgaaa atagcctatg
     4621 ctatcttacg tgatcgctta aatttatcta ttaaaaatag ctgggttgat gaagatggta
     4681 acatttactt tgtttattca aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa
     4741 agttaacaaa aataaaaaaa gggttagaaa acgatggtct actcattcaa aaacgacgtg
     4801 gtttaaataa accaaatatt ctatatttaa tgaaacctat tgtcacagaa cgtgatattt
     4861 acaaaataga aaaagaagaa aacgatgttg aaccttacgg cgagaaggaa gttcgaaaat
     4921 cgaacgtcca gaagttcgaa aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag
     4981 ttcgaaaatc gaacactaat gatactgact ttaatgacat tgactttaat gatactgaga
     5041 ataatgatat gaatgacttg aatgatataa agtacaaaaa tgaaattagt aatcattcaa
     5101 atcattcaaa tcaatttaca cataattttg atgataaaga aatgctattg caagaattcc
     5161 cagaacaact aactaattat ttattaaaat atgattatag agatttagaa atcataaagg
     5221 ctgttattct taaagcaaaa aaatcattta attcaaggca tgaagatatg cattacatgt
     5281 tagaggacat tgaggatgaa atattaactt ctttaaaaag acttaaaaaa gctatacatg
     5341 ataggggagt taaaggccaa aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa
     5401 caattctaac tgaacttgaa gaaacacatg ccctttatat gcgccgtaaa aacatgaaac
     5461 aatataatat ctttaatcaa taattcaaat gattttataa cactcgaaga gacatcaaaa
     5521 tattccgtta aatcatctga ctttttaact gatgacgacg aaaaaaatca agaaattgta
     5581 agtgatttag aaaaaggttt gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa
     5641 caaaagcata aaattttaaa cttagacgat gccgaagatg gtaatttgat taatacaagt
     5701 gatgaagata aaacgacaga cgaagaaaaa aagcacattc aattaccgca atttggaatt
     5761 tcgaaaagca aaattattat ttaagacatt agtgataact gatgtctttt tttattgttt
     5821 ttctgtaaaa aagtgctgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt
     5881 gtgacactac agctttgtat gatatcactt taaaataact taaaaccctt ggaatgtctg
     5941 gccttgccag atctatcatt tttgaatgat agcaaatttc ccttatgctc ttacggagat
     6001 tttagagtta aattaaaatt tctaagtttt tgataagaaa agccctgcag gaatttagaa
     6061 aaacatgggg aagttttaaa gtattttatg ctaattttta aatttgcatg taaatgggca
     6121 gtatctaaaa aattagtcac tgattttgct gaaatttttg tttgtgaaat tagcatatat
     6181 ttatttggct catatttgcg ttttaagaga ctgtatagat ttttaagtac aaaacctata
     6241 tgatttgcct ctaagtcttc aaaatgcctc ttaaaatcca aaataaaagt gtttaatatt
     6301 gtaataagga ttgctatttt tataaaagga gagatatcat gaagattaaa actttcgcta
     6361 cttttactca tcgtcaatta gataaacaag ttaataggtt tttaaagaat gatattcatg
     6421 tattagaaat tcaaacacac agtaattggt tatatatttc agctattgtt ttttatgagg
     6481 ataaataagg aacatcgtat ttatgccgaa aaaatttatt gatgttgaga agaaccctta
     6541 actaaacttg cagacgaatg tcggcatagc gtgagctatt aagccgacca ttcgacaagt
     6601 tttgggattg ttaagggttc cgaggctcaa cgtcaataaa acaattggaa taaaatcaac
     6661 ctacagagca tccagctgca aatgcagttc cgcaaactgc ttcacataca ccgccggcta
     6721 ttggatttac gattgcccat actccacagt agtgagcaca tgcataaagt ccagccatac
     6781 tacaagcgaa tgctttgccg ttccatttga atcctgcata tgttaccggt ctgtttttat
     6841 caactttttc agccttatat tcaacgtaat ttttgttaat aagtattttt ccctctttgt
     6901 tttcagtcat acttcttttt tcggcaatga attttgtgat ttttttgttg tagttatcat
     6961 atattgtttc agaaactaca atttcttttg ttttttcatt tttataagcg ttattagcta
     7021 tgtagccata tgcattttta tcatttgctt ctgtagctag atattttaat ttagaatttt
     7081 tgtcttctag ctttattttt ttaaatcctt cttttttcat ttgcttttcg aatttttgca
     7141 tatcttcatt tttattttgc aattgttcta gaaaacttat ttctttcttg gtcatttgtg
     7201 atacttcgat gtttttgctt ttcaattttt ctttttgatt ttctagtaaa gaactttttt
     7261 gttcttcatt tgcgtaagca ttagaaaaat tcgcgctaaa taccagtagg aacaaggaaa
     7321 ttattgaaaa taattttata attttcattt gatacctcca agataataag atattttaat
     7381 tgtataagta agcgctttat aaaatcaata tttattttaa tatatttata aacatatttt
     7441 tgaaaggagt ctattttatg aatgaaaatt tagaaaaata tataaaaagt ttgccacttt
     7501 taggtttgtt aattagtatt ttgttaattg ttttattctt ttttatttta gatgttgatg
     7561 aagatttatt cgtggttttt ttatactgtt tattgccttt gatagttaat ttgtctattt
     7621 atggttttta tgtttttgtt aaaagaaagt aagattatga ataattttgt ttttaatttg
     7681 tatgctttga ggggttggaa aacggtaggt gtatagatta gatttcttgt ttttttgaaa
     7741 cgagtgtgaa cgagtttctt cttgtcttga tttttgataa aaacgcccta caggaattta
     7801 gaaaaacgtg gggaagtttt aaagaatttt atgctaattt ttaatttgca tgtaaatggg
     7861 cagtgtctaa aaatagttat tgtttttgct caaatttttg tttttgaaaa tagcatatat
     7921 ttatttggct catatttgcg ttttaaaagc atatataagt tttagatata aaactatatg
     7981 aatcacccct aaatctttaa aatgccctct taaaattcaa aataagggca taaaaaataa
     8041 aagctacccc agataaagtg aggtagcttt ttctttcata cggtctattt atttttcttt
     8101 cgacgccgta tcgtcgatgt aattcaaaaa agttatgggc tataaatcta catcactttt
     8161 tcaagaatgt agtagcaata ttcaacgcaa attaattttt ataactgctc ggaatatctc
     8221 aagccgtttc tttaattttg aaataaaaaa atcgacgaag gtcgattacg tttttgcaca
     8281 tgtcgattta tccgacgtat aagtgcgccc ttacgggatt taactagatt ataacgacga
     8341 attttagacc tgtaaagcaa taacaattag gtctaccagg tagataaagt gtacaaagta
     8401 gaaccgaaaa agttatttat tttgaagatt tttcgtagat ataatttgct aataaacttc
     8461 ctaaaactga ataaagaaaa atagttaaac tcatgtaatc actccataat tctgaagtgt
     8521 ctccatgtct acgcatagac acgcatttat gccgaaaaaa tttattgatg ttgagaagaa
     8581 cccttaacta aacttgcaga cgaatgtcgg catagcgtga gctattaagc cgaccattcg
     8641 acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa
     8701 gcacctatga ttatttaaaa atataagtta taatacattc aaaaatattt ttgaatgagg
     8761 tgtattatga ttcaaacggt tgttgctgct gctgttcttt atattgctac agcagttgat
     8821 ttattagtaa ttttattaat attttttgct agagcaaaga ctagaaaaga atatagagat
     8881 atttatgttg gtcaatattt agggtctatt attttaatat tagttagttt gtttttagct
     8941 tttgtattaa attatgttcc agaaaagtgg atattaggtt tattgggttt aataccaatt
     9001 tatcttggaa ttaaagtggc tatttatgat gattgtgaag gagaaaagag agctaaaaaa
     9061 gaattgaatg aaaaaggatt gtctaaatta gttggtacgg ttgcaattgt tacgatagca
     9121 agttgtggcg ccgataatat tggtttattt gttccgtatt ttgtgacatt aagtgttact
     9181 aatttattac ttactctgtt tgtcttttta attttaattt tcttcttggt gtttactgca
     9241 caaaaattag ctaatattcc aggaattgga gaaattgttg agaaatttag tcgttggatt
     9301 atggctatta tttatatagc tttaggttta tttattatta ttgaaaatga cactattcaa
     9361 acaattttag gatttatatt ttaatttagg gtgtgatttt atatgagtta tgaaaatact
     9421 tgtgatgtga tctgtgtaca tgaggataaa gttaacaatg ctttaagttt tttagaagat
     9481 gataaatcta agaaattact taacatttta gaaaaaattt gtgatgagaa gaaattgaaa
     9541 atcatattat ctttgattaa agaagatgag ttgtgtgttt gtgatatttc tttgatattg
     9601 aaaatgagtg ttgcttcaac ttcacatcat ttaaggcttt tatataaaaa tgaggtactt
     9661 gatttttata aagatggaaa gatggcatat tattttatta aagacgatga aataagagag
     9721 tttttctcta aaaatcatga gggtttttga aacgagtttc ttcttgtctt gatactataa
     9781 aaaacaaagt tatataacat aagacgactt atcggaagta gatatgcttg tatattcaca
     9841 gatggtataa taatagagtc gcctatctct caggcgtcaa ttgacgaaga gaggaggtgc
     9901 attactttgc taatcatttt cgttcacatc atagcaccag tcatcagtgg ctgtgctgtt
     9961 gcgtattata cttattggct tagtaaacgc aacaaataat taataggcga ctttataagc
    10021 cagcatcgaa aaaatcccca cactactgca atagtgtggg gattggtgca ttaattgtta
    10081 atcattttcg ttatataaat tataacactg gttagtagag aaatgcaaaa tgattgaatt
    10141 aatagtaaat gtattaatga atactcacac ttatactttt aaaatagttc ttcctaaatg
    10201 ttgtcctttt aaaagctgat ctatcgtttt atagaattct tcaaatccaa tttcattgta
    10261 tagaagtgaa tttcctatat tccattcatt tgctaactta ctccatatat cttcacggat
    10321 attatgatca acatatacag aatcaatgcc aattaattta atacctctaa gaataaacgg
    10381 taatatatta gcgttaagtg aatttcctgc tacatttcca catacagtag caacaccttg
    10441 ataatttaga cgttttaaac cgtataatgt tacattactc cccactgtat ctaagaaata
    10501 atcaaaaata ccttttttaa gcggtttatt atcatcttca attattatta cttgttttgc
    10561 acctaatttt ttttgcgatt tctatttgct tttcttttct tacgatagca gtgatatttt
    10621 catatccaat tttatgtaaa atttgtatag caacactgcc cacaccacct gtagcccaga
    10681 cactaaaatt tcaggttgtg atttgatatt cattccatat ttttctagtt caaaaattga
    10741 taatgctgcc gtaaaaccgg ctgtgccata taccatagac tctttaatag acatttcttg
    10801 gggaatagga acaatccact ctccaggcac ttgtgcatat tctgaaaatc cacctgtatg
    10861 tgtcacgcca acatcataac cagtaactaa tacattatcg ccttctttaa acttatcatt
    10921 cgaacttgat tcaacaatac ctgcaaaatc tataccagga atcataggat aatctctaat
    10981 tacaccgcct ttatattgta aagctagcat atccttataa tttattgaag agtataccac
    11041 ttttactaat acatcaccct tcgataaatg agattcatca actttttcta ctttataact
    11101 aacatttgat gcactttttc tcactactac cgcattgaac atttattagc cttctttcac
    11161 aattgttttg tagaagtatg tttttgttgc aaaactattt tcctacaaaa cttatcatga
    11221 tatgataagc gagtcaatac taatgattta agagaggttt tagttgtgga attagataat
    11281 tgtataaatt atttgttgag tacttctcag aatagagttt ttaaacattt taaaagttta
    11341 ttaagtccct ataatattac tccagcagaa tatggtgttt tgaattgctt gattaatagt
    11401 aatttgaata cacctaaagc gattggtaat actttaaatt tagaaccttc aacaatatct
    11461 ggaatattag ataagatgtc caaaaaggat ctaataacac gtcaaactga taatgaaaat
    11521 agaagaactg tgctaataaa cagtacaaat aaagcaaaag acttatggcc aacattagaa
    11581 aagaaagcct atcaacttaa taatcattac ttcgctaatt taactaatga agaaactgta
    11641 gtgtttaaaa agatattact aaagataaat aaaactacgt tttaattttt acgttataaa
    11701 aaagttgaag tgcgaattat aatcgagtaa atataaatga tagcttagct taattatata
    11761 ttaaagccaa ctatactttt attcccgaaa aacctactat ccaaatttct aagatacttt
    11821 agcctctata ataaacatat tgtaatataa tttatgtatt atttaggttt accctaatcg
    11881 aacaataaaa aatacttgta aaatgctatt gtcactttgc ttgatatatg agaattattt
    11941 aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct ttttcgattg
    12001 atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat atacaatatt
    12061 tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg gaaaatcagt
    12121 ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa actataagat
    12181 gttatcagta tttattatgc atttagaata ttaaaattcc ttcattacac tcttggcggt
    12241 ttcacttatc aacttatcat ttggcttatc acttttattg tctttattcg taaaaatgac
    12301 taaaacaata ggttcagatt ggcccttagg ataaacaaaa gcaacatcat ttctagaagc
    12361 atatgttatt gcttgaccac ttttatcagc aaccttatag tcttttggaa caccatcttt
    12421 aattaaagtg tctccgtttt tattattaaa cattaaatca agtaagaaat ttttattttt
    12481 tttgcttaat tttccatttg cgataagttt atttaaagtc ttgccgaaag cagcaggcgt
    12541 tgaagtatct tttttgctct ttggtgaata gtaatttaat tctatctcat atctaactgg
    12601 atttgttact ttatctccta gttcttttag acgttgttta acttttttga ttccaccgat
    12661 ttcttttata attttattgt ttgctgtatt atcactatat gtcattgaag cctcaataag
    12721 ttcttttaaa gtgatatctt ttcctacata tttttctaaa ataggagaat aagcaactat
    12781 atcatctttg ttaatatgta cttttttatt taacttatta taaggtactt gttctaacaa
    12841 aatagcacta tttatcgctt ttgaagtcga agcataggca aatctcttat ctgaattaaa
    12901 ttttacttcc ttaccacttt tagtatttaa agcataaaca ccaatatgag cattatattt
    12961 tttttctaaa tcatttaact ctttggcatg tgaactgttt gaattacatg cacttaaaac
    13021 taaagcaatt acaattaaaa atattaactt tttcaaaata aaccctccga tattacagtt
    13081 gtaattttta taaatcaata atattacaat tgtaatattg atgtcaattg tttattataa
    13141 atatttgaaa gaaggtttca aaa
//
