LOCUS       CP051969               14307 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain ER09615.3 plasmid pER09615.3A.2,
            complete sequence.
ACCESSION   CP051969
VERSION     CP051969.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN14669408
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.290803767661x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/23/2020 09:58:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,926
            CDSs (total)                      :: 2,844
            Genes (coding)                    :: 2,781
            CDSs (with protein)               :: 2,781
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 43 of 63
            Pseudo Genes (incomplete)         :: 23 of 63
            Pseudo Genes (internal stop)      :: 10 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER09615.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER09615.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2017"
                     /collected_by="Mount Sinai Hospital"
     gene            291..1274
                     /locus_tag="HIK68_14550"
     CDS             291..1274
                     /locus_tag="HIK68_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QOB40069.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            1267..1583
                     /locus_tag="HIK68_14555"
                     /pseudo=""
     CDS             1267..1583
                     /locus_tag="HIK68_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2130..2279
                     /locus_tag="HIK68_14560"
     CDS             2130..2279
                     /locus_tag="HIK68_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40070.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(2447..3139)
                     /locus_tag="HIK68_14565"
     CDS             complement(2447..3139)
                     /locus_tag="HIK68_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40071.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            3249..3443
                     /locus_tag="HIK68_14570"
     CDS             3249..3443
                     /locus_tag="HIK68_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40072.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4559..5176
                     /gene="cadD"
                     /locus_tag="HIK68_14575"
     ARG             4560..5173
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5195..5542
                     /locus_tag="HIK68_14580"
     CDS             5195..5542
                     /locus_tag="HIK68_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QOB40074.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5699..5791
                     /locus_tag="HIK68_14585"
     CDS             5699..5791
                     /locus_tag="HIK68_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QOB40075.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5963..6934)
                     /locus_tag="HIK68_14590"
     CDS             complement(5963..6934)
                     /locus_tag="HIK68_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QOB40076.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            7058..7477
                     /locus_tag="HIK68_14595"
     CDS             7058..7477
                     /locus_tag="HIK68_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QOB40077.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(8003..8848)
                     /gene="blaZ"
                     /locus_tag="HIK68_14600"
     ARG             complement(8007..8848)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8955..10712
                     /gene="blaR1"
                     /locus_tag="HIK68_14605"
     ARG             8956..10709
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10702..11082
                     /gene="blaI"
                     /locus_tag="HIK68_14610"
     ARG             10703..11079
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            11346..11924
                     /locus_tag="HIK68_14615"
     CDS             11346..11924
                     /locus_tag="HIK68_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QOB40081.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(12147..12332)
                     /locus_tag="HIK68_14620"
     CDS             complement(12147..12332)
                     /locus_tag="HIK68_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40082.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTILI
                     LLTSIITVVSVNDEDT"
                     /name="hypothetical protein"
     gene            12594..13268
                     /locus_tag="HIK68_14625"
     CDS             12594..13268
                     /locus_tag="HIK68_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="QOB40083.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(13429..14172)
                     /locus_tag="HIK68_14630"
     CDS             complement(13429..14172)
                     /locus_tag="HIK68_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QOB40084.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             complement(8004..8848)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1640..1787
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          292..1274
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1406..1575
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
     Typing          complement(13430..14172)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatatttatc aagggtttat aaacccttga taaagctata ttttcccttt tataagttta
       61 tttaaagtgc tataataaac ttaatataac aaaaacccca actactggta atagtcgggg
      121 tttggtaata aaacgtttag aaactgttta tttatataat tttcgatact tgtattatat
      181 aatgaataat atcttagtgc aagcacctaa atcattaaat aaatgtaaag ctaaataaaa
      241 acgttttatt acctcttacc aaactaaaat atggaaagag gttttttgtc atgcaaaatc
      301 aatattttac agtgcaagaa aattacaaag aaagatttta tcaaatccct aaagtattct
      361 ttaccagtga aaattataaa aatctaacta atgatatgaa aatagcctat gctatcttac
      421 gtgatcgctt aaatttatct attaaaaata gctgggttga tgaagatggt aacatttact
      481 ttgtttattc aaatgaaaag ctaatggaaa tcttaaattg taaaaaagaa aagttaacaa
      541 aaataaaaaa agggttagaa aacgatggtc tactcattca aaaacgacgt ggtttaaata
      601 aaccaaatat tctatattta atgaaaccta ttgtcacaga acgtgatatt tacaaaatag
      661 aaaaagaaga aaacgatgtt gaaccttacg gcgagaagga agttcgaaaa tcgaacgtcc
      721 agaagttcga aaatcgaacg tccagaagtt cgaaaatcga acgtccagaa gttcgaaaat
      781 cgaacactaa tgatactgac tttaatgaca ttgactttaa tgatactgag aataatgata
      841 tgaatgactt gaatgatata aagtacaaaa atgaaattag taatcattca aatcattcaa
      901 atcaatttac acataatttt gatgataaag aaatgctatt gcaagaattc ccagaacaac
      961 taactaatta tttattaaaa tatgattata gagatttaga aatcataaag gctgttattc
     1021 ttaaagcaaa aaaatcattt aattcaaggc atgaagatat gcattacatg ttagaggaca
     1081 ttgaggatga aatattaact tctttaaaaa gacttaaaaa agctatacat gataggggag
     1141 ttaaaggcca aaaggaaaca atcaagtcaa tgcaagctta tttaatgcaa acaattctaa
     1201 ctgaacttga agaaacacat gccctttata tgcgccgtaa aaacatgaaa caatataata
     1261 tctttaatca ataattcaaa tgattttata acactcgaag agacatcaaa atattccgtt
     1321 aaatcatctg actttttaac tgatgacgac gaaaaaaatc aagaaattgt aagtgattta
     1381 gaaaaaggtt tgtatcgaaa acgtatgtta agttatggtg gattgcttaa acaaaagcat
     1441 aaaattttaa acttagacga tgccgaagat ggtaatttga ttaatacaag tgatgaagat
     1501 aaaacgacag acgaagaaaa aaagcacatt caattaccgc aatttggaat ttcgaaaagc
     1561 aaaattatta tttaagacat tagtgataac tgatgtcttt ttttattgtt tttctgtaaa
     1621 aaagtgctgt cttatttttg tgacaaatgc tgtatgtagt gtcacaaaag tgtgacacta
     1681 cagctttgta tgatatcact ttaaaataac ttaaaaccct tggaatgtct ggccttgcca
     1741 gatctatcat ttttgaatga tagcaaattt cccttatgct cttacggaga ttttagagtt
     1801 aaattaaaat ttctaagttt ttgataagaa aagccctgca ggaatttaga aaaacatggg
     1861 gaagttttaa agtattttat gctaattttt aaatttgcat gtaaatgggc agtatctaaa
     1921 aaattagtca ctgattttgc tgaaattttt gtttgtgaaa ttagcatata tttatttggc
     1981 tcatatttgc gttttaagag actgtataga tttttaagta caaaacctat atgatttgcc
     2041 tctaagtctt caaaatgcct cttaaaatcc aaaataaaag tgtttaatat tgtaataagg
     2101 attgctattt ttataaaagg agagatatca tgaagattaa aactttcgct acttttactc
     2161 atcgtcaatt agataaacaa gttaataggt ttttaaagaa tgatattcat gtattagaaa
     2221 ttcaaacaca cagtaattgg ttatatattt cagctattgt tttttatgag gataaataag
     2281 gaacatcgta tttatgccga aaaaatttat tgatgttgag aagaaccctt aactaaactt
     2341 gcagacgaat gtcggcatag cgtgagctat taagccgacc attcgacaag ttttgggatt
     2401 gttaagggtt ccgaggctca acgtcaataa aacaattgga ataaaatcaa cctacagagc
     2461 atccagctgc aaatgcagtt ccgcaaactg cttcacatac accgccggct attggattta
     2521 cgattgccca tactccacag tagtgagcac atgcataaag tccagccata ctacaagcga
     2581 atgctttgcc gttccatttg aatcctgcat atgttaccgg tctgttttta tcaacttttt
     2641 cagccttata ttcaacgtaa tttttgttaa taagtatttt tccctctttg ttttcagtca
     2701 tacttctttt ttcggcaatg aattttgtga tttttttgtt gtagttatca tatattgttt
     2761 cagaaactac aatttctttt gttttttcat ttttataagc gttattagct atgtagccat
     2821 atgcattttt atcatttgct tctgtagcta gatattttaa tttagaattt ttgtcttcta
     2881 gctttatttt tttaaatcct tcttttttca tttgcttttc gaatttttgc atatcttcat
     2941 ttttattttg caattgttct agaaaactta tttctttctt ggtcatttgt gatacttcga
     3001 tgtttttgct tttcaatttt tctttttgat tttctagtaa agaacttttt tgttcttcat
     3061 ttgcgtaagc attagaaaaa ttcgcgctaa ataccagtag gaacaaggaa attattgaaa
     3121 ataattttat aattttcatt tgatacctcc aagataataa gatattttaa ttgtataagt
     3181 aagcgcttta taaaatcaat atttatttta atatatttat aaacatattt ttgaaaggag
     3241 tctattttat gaatgaaaat ttagaaaaat atataaaaag tttgccactt ttaggtttgt
     3301 taattagtat tttgttaatt gttttattct tttttatttt agatgttgat gaagatttat
     3361 tcgtggtttt tttatactgt ttattgcctt tgatagttaa tttgtctatt tatggttttt
     3421 atgtttttgt taaaagaaag taagattatg aataattttg tttttaattt gtatgctttg
     3481 agggggttgg aaaacggtag gtgtatagat tagatttctt gtttttttga aacgagtgtg
     3541 aacgagtttc ttcttgtctt gatttttgat aaaaacgccc tacaggaatt tagaaaaacg
     3601 tggggaagtt ttaaagaatt ttatgctaat ttttaatttg catgtaaatg ggcagtgtct
     3661 aaaaatagtt attgtttttg ctcaaatttt tgtttttgaa aatagcatat atttatttgg
     3721 ctcatatttg cgttttaaaa gcatatataa gttttagata taaaactata tgaatcaccc
     3781 ctaaatcttt aaaatgccct cttaaaattc aaaataaggg cataaaaaat aaaagctacc
     3841 ccagataaag tgaggtagct ttttctttca tacggtctat ttatttttct ttcgacgccg
     3901 tatcgtcgat gtaattcaaa aaagttatgg gctataaatc tacatcactt tttcaagaat
     3961 gtagtagcaa tattcaacgc aaattaattt ttataactgc tcggaatatc tcaagccgtt
     4021 tctttaattt tgaaataaaa aaatcgacga aggtcgatta cgtttttgca catgtcgatt
     4081 tatccgacgt ataagtgcgc ccttacggga tttaactaga ttataacgac gaattttaga
     4141 cctgtaaagc aataacaatt aggtctacca ggtagataaa gtgtacaaag tagaaccgaa
     4201 aaagttattt attttgaaga tttttcgtag atataatttg ctaataaact tcctaaaact
     4261 gaataaagaa aaatagttaa actcatgtaa tcactccata attctgaagt gtctccatgt
     4321 ctacgcatag acacgcattt atgccgaaaa aatttattga tgttgagaag aacccttaac
     4381 taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatt cgacaagttt
     4441 tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata aagcacctat
     4501 gattatttaa aaatataagt tataatacat tcaaaaatat ttttgaatga ggtgtattat
     4561 gattcaaacg gttgttgctg ctgctgttct ttatattgct acagcagttg atttattagt
     4621 aattttatta atattttttg ctagagcaaa gactagaaaa gaatatagag atatttatgt
     4681 tggtcaatat ttagggtcta ttattttaat attagttagt ttgtttttag cttttgtatt
     4741 aaattatgtt ccagaaaagt ggatattagg tttattgggt ttaataccaa tttatcttgg
     4801 aattaaagtg gctatttatg atgattgtga aggagaaaag agagctaaaa aagaattgaa
     4861 tgaaaaagga ttgtctaaat tagttggtac ggttgcaatt gttacgatag caagttgtgg
     4921 cgccgataat attggtttat ttgttccgta ttttgtgaca ttaagtgtta ctaatttatt
     4981 acttactctg tttgtctttt taattttaat tttcttcttg gtgtttactg cacaaaaatt
     5041 agctaatatt ccaggaattg gagaaattgt tgagaaattt agtcgttgga ttatggctat
     5101 tatttatata gctttaggtt tatttattat tattgaaaat gacactattc aaacaatttt
     5161 aggatttata ttttaattta gggtgtgatt ttatatgagt tatgaaaata cttgtgatgt
     5221 gatctgtgta catgaggata aagttaacaa tgctttaagt tttttagaag atgataaatc
     5281 taagaaatta cttaacattt tagaaaaaat ttgtgatgag aagaaattga aaatcatatt
     5341 atctttgatt aaagaagatg agttgtgtgt ttgtgatatt tctttgatat tgaaaatgag
     5401 tgttgcttca acttcacatc atttaaggct tttatataaa aatgaggtac ttgattttta
     5461 taaagatgga aagatggcat attattttat taaagacgat gaaataagag agtttttctc
     5521 taaaaatcat gagggttttt gaaacgagtt tcttcttgtc ttgatactat aaaaaacaaa
     5581 gttatataac ataagacgac ttatcggaag tagatatgct tgtatattca cagatggtat
     5641 aataatagag tcgcctatct ctcaggcgtc aattgacgaa gagaggaggt gcattacttt
     5701 gctaatcatt ttcgttcaca tcatagcacc agtcatcagt ggctgtgctg ttgcgtatta
     5761 tacttattgg cttagtaaac gcaacaaata attaataggc gactttataa gccagcatcg
     5821 aaaaaatccc cacactactg caatagtgtg gggattggtg cattaattgt taatcatttt
     5881 cgttatataa attataacac tggttagtag agaaatgcaa aatgattgaa ttaatagtaa
     5941 atgtattaat gaatactcac acttatactt ttaaaatagt tcttcctaaa tgttgtcctt
     6001 ttaaaagctg atctatcgtt ttatagaatt cttcaaatcc aatttcattg tatagaagtg
     6061 aatttcctat attccattca tttgctaact tactccatat atcttcacgg atattatgat
     6121 caacatatac agaatcaatg ccaattaatt taatacctct aagaataaac ggtaatatat
     6181 tagcgttaag tgaatttcct gctacatttc cacatacagt agcaacacct tgataattta
     6241 gacgttttaa accgtataat gttacattac tccccactgt atctaagaaa taatcaaaaa
     6301 tacctttttt aagcggttta ttatcatctt caattattat tacttgtttt gcacctaatt
     6361 tttttgcgat ttctatttgc ttttcttttc ttacgatagc agtgatattt tcatatccaa
     6421 ttttatgtaa aatttgtata gcaacactgc ccacaccacc tgtagcccca gacactaaaa
     6481 tttcaggttg tgatttgata ttcattccat atttttctag ttcaaaaatt gataatgctg
     6541 ccgtaaaacc ggctgtgcca tataccatag actctttaat agacatttct tggggaatag
     6601 gaacaatcca ctctccaggc acttgtgcat attctgaaaa tccacctgta tgtgtcacgc
     6661 caacatcata accagtaact aatacattat cgccttcttt aaacttatca ttcgaacttg
     6721 attcaacaat acctgcaaaa tctataccag gaatcatagg ataatctcta attacaccgc
     6781 ctttatattg taaagctagc atatccttat aatttattga agagtatacc acttttacta
     6841 atacatcacc cttcgataaa tgagattcat caactttttc tactttataa ctaacatttg
     6901 atgcactttt tctcactact accgcattga acatttatta gccttctttc acaattgttt
     6961 tgtagaagta tgtttttgtt gcaaaactat tttcctacaa aacttatcat gatatgataa
     7021 gcgagtcaat actaatgatt taagagaggt tttagttgtg gaattagata attgtataaa
     7081 ttatttgttg agtacttctc agaatagagt ttttaaacat tttaaaagtt tattaagtcc
     7141 ctataatatt actccagcag aatatggtgt tttgaattgc ttgattaata gtaatttgaa
     7201 tacacctaaa gcgattggta atactttaaa tttagaacct tcaacaatat ctggaatatt
     7261 agataagatg tccaaaaagg atctaataac acgtcaaact gataatgaaa atagaagaac
     7321 tgtgctaata aacagtacaa ataaagcaaa agacttatgg ccaacattag aaaagaaagc
     7381 ctatcaactt aataatcatt acttcgctaa tttaactaat gaagaaactg tagtgtttaa
     7441 aaagatatta ctaaagataa ataaaactac gttttaattt ttacgttata aaaaagttga
     7501 agtgcgaatt ataatcgagt aaatataaat gatagcttag cttaattata tattaaagcc
     7561 aactatactt ttattcccga aaaacctact atccaaattt ctaagatact ttagcctcta
     7621 taataaacat attgtaatat aatttatgta ttatttaggt ttaccctaat cgaacaataa
     7681 aaaatacttg taaaatgcta ttgtcacttt gcttgatata tgagaattat ttaaccttat
     7741 aaatgagaaa aaagcaacgc actttaaata agatacgttg ctttttcgat tgatgaacac
     7801 ctataattaa actattcatc tattatttat gattttttgt atatacaata tttctagttt
     7861 gttaaagaga attaagaaaa taaatctcga aaataataaa gggaaaatca gtttttgata
     7921 tcaaaattat acatgtcaac gataatacaa aatataatac aaactataag atgttatcag
     7981 tatttattat gcatttagaa tattaaaatt ccttcattac actcttggcg gtttcactta
     8041 tcaacttatc atttggctta tcacttttat tgtctttatt cgtaaaaatg actaaaacaa
     8101 taggttcaga ttggccctta ggataaacaa aagcaacatc atttctagaa gcatatgtta
     8161 ttgcttgacc acttttatca gcaaccttat agtcttttgg aacaccatct ttaattaaag
     8221 tgtctccgtt tttattatta aacattaaat caagtaagaa atttttattt tttttgctta
     8281 attttccatt tgcgataagt ttatttaaag tcttgccgaa agcagcaggc gttgaagtat
     8341 cttttttgct ctttggtgaa tagtaattta attctatctc atatctaact ggatttgtta
     8401 ctttatctcc tagttctttt agacgttgtt taactttttt gattccaccg atttctttta
     8461 taattttatt gtttgctgta ttatcactat atgtcattga agcctcaata agttctttta
     8521 aagtgatatc ttttcctaca tatttttcta aaataggaga ataagcaact atatcatctt
     8581 tgttaatatg tactttttta tttaacttat tataaggtac ttgttctaac aaaatagcac
     8641 tatttatcgc ttttgaagtc gaagcatagg caaatctctt atctgaatta aattttactt
     8701 ccttaccact tttagtattt aaagcataaa caccaatatg agcattatat tttttttcta
     8761 aatcatttaa ctctttggca tgtgaactgt ttgaattaca tgcacttaaa actaaagcaa
     8821 ttacaattaa aaatattaac tttttcaaaa taaaccctcc gatattacag ttgtaatttt
     8881 tataaatcaa taatattaca attgtaatat tgatgtcaat tgtttattat aaatatttga
     8941 aagaaggtgt caaaatggct aaattactaa taatgagcgt agtaagcttt tgttttatat
     9001 ttctattgtt agtatttttt aggtacatat taaaacgcta ttttaattat agtttaaatt
     9061 ataaagtttg gtatctaact atgcttgctg gattaattcc ttttatccca attaaattct
     9121 cttttataaa atttaataat gtgaataatc aagcgcccac agttgaaagt aagtcacacg
     9181 atttgaacca taacataaat accaccaaac ctattcaaga gttcacaaca gatatccata
     9241 agtttaattg ggattcaatt gataatatct gcacagttat ttggatagtt ttagttatta
     9301 ttttaagttt taaatttttg aaagccttat tatatcttaa atatttaaag aaacagtcac
     9361 tttatctaaa cgaaaatgaa aaaaataaaa tagatacgat acttttcaac catcaatata
     9421 aaaaaaatat tgtgattcga aaagcagagg ctattcaatc tccaataact ttttggtatg
     9481 ggaaatatat tattttgatt cctagttcat attttaaaag tgtaattgat aaaagactaa
     9541 aatatataat actgcatgaa tatgctcatg ctaaaaatag agatacttta catttaatta
     9601 tctttaatat cttctctatt attatgagtt acaatccatt agtacatatt gtaaaaagaa
     9661 agataattca tgacaatgaa gtagaagccg atagatttgt ccttaataat attaacaaaa
     9721 atgaatttaa aacttatgcg gaatctatta tggactccgt attaaaaacc ccattttcca
     9781 acaaaaatat attaagccat tcatttaatg gtaaaaaatc attactcaaa agtagattaa
     9841 ttaatataaa agaagccgat ctgaaaaagc agtcaaaatt aattctaatt tttatttgta
     9901 tttttacttt ttttatcatg ataattcaaa gccagttttt aatggggcaa tccttaactg
     9961 attataatta taaaaagccc ttacaaagcg attaccaaat tttagatgaa agtaaaaatt
    10021 ttggttcaaa tagtggatct ttcgttatgt acagcatgaa gaaagacaaa tactatattt
    10081 ataatgaaaa agaaagtaga aaaagatatt ctcctgattc aacttacaaa atttatttag
    10141 cactgtttgg acttgaccgt catattataa gtgataaaaa ttcacgtatg tcatggaatc
    10201 ataaacatta tcttttcgag tcttggaata aggaacaaga tttaaataca gcaatgcaaa
    10261 attcagttaa ttggtacttc gaacgtatta gtaatcaaat accaaagaac tatactgcgg
    10321 ctcaactcaa gcaattaaat tatggtaatg aaaatttggg aagttataaa agctattgga
    10381 tggaagatag tttgaaaata tctaatcttg aacaagtaat agtttttaaa aatatgatgg
    10441 aacaaaataa ccattttagt aaaaaagcaa agaatcaatt atcttcttca ttattgatta
    10501 agaaaaatga aaagtatgaa ctgtatggga aaacaggtac aggtatagta aacgggaagt
    10561 ataataatgg gtggtttgta ggttacgtaa ttacaaatca tgataagtat tattttgcta
    10621 cacatttatc agatggaaac ccgtctggga aaaatgctga attaataagt gaaaaaatat
    10681 taaaaggaat gggtgtttta aatgaccaat aagcaagttg aaatatctat ggctgaatgg
    10741 gatgttatga atataatatg ggataaaaaa tcagtatcag ctaatgaaat tgtagttgaa
    10801 attcaaaaat ataaagaagt tagtgataaa acgattagaa cattaatcac aagactatat
    10861 aaaaaagaga ttataaaacg atacaaatca gaaaatattt atttttactc atcaaatatt
    10921 aaagaagacg atattaaaat gaaaactgct aaaacctttc ttaataaact gtatggaggg
    10981 gatatgaaaa gtttagtgct gaattttgcg aaaaatgaag aattaaataa caaagaaatt
    11041 gaagaattgc gagacatttt aaatgatatt agtaaaaagt aattagaggt atgtttcaaa
    11101 cataatacat atgtaaacca tacttgactc ttattaaatt agttttgtta atgacattta
    11161 aaatatatta ttaaagatga gagtgtccag aaaataacca ttttctgtac actcttgttt
    11221 tttgtctaaa taagtaagta ttacatagaa ttatgtgttc aaaaacccaa caaaaaatct
    11281 atgttcacaa aagacctcgt aatcaagtta tggtacacta aaggtgtata taggaggtct
    11341 gtattttgaa aataggttat gcaagagttt caactggatt acaaaatttg aatttacagg
    11401 aggatcgatt aaatacatat ggttgtgaaa agatatttaa tgaccatatg agtggttcaa
    11461 aaagtaagcg acctggttta gataaagcaa ttgaatttgc gaggtcagga gatacgattg
    11521 ttgtttggag attagataga ctaggacgga acatggagga tttaatcacg ttagttaatg
    11581 aacttaacga acgaggtgtg agttttcata gtttagaaga aaatataacg atggataaat
    11641 caagttctac aggacaatta ttatttcatt tattcgcagc atttgcagag tttgaacgta
    11701 atttaatttt agagcgatca tcagccggtc gaattgcagc acgtgctaga ggccgatatg
    11761 gaggtagacc tgaaaaatta aataaacaag atttaaactt acttaagaca ctttatgata
    11821 atggtacgcc aattaaaaca attgcagagc agtggcaagt ttcacgtaca actatttatc
    11881 gttatctcaa taaattagag gcaaaagaga ataaaaacaa gtagagcaac taatctagct
    11941 tactataatg aatctactaa ataattttat atatcattaa catacaaaga ctttgattca
    12001 atgtcactcg atgtaaaaga gacgttctga cagcgtctaa aatctcataa tattttcctg
    12061 ttatgagatt ttaataataa cacaagaaaa aagcgtacaa attcgtacgc ttttttaata
    12121 taatacctgt ttcactaatg aacgttttaa gtatcttcat cattaactga aactacagta
    12181 ataatactcg tcaacaatat tagtatagtt aaaatagaaa gacttaggac tggcataacc
    12241 gaactaaaat tatatttgaa atataaaagt gctaatgaag agagaaatgt aaaaataata
    12301 aataccaaag accatttttc taatttattc attggcttca cctctttgaa cttttatata
    12361 atcataggag atatttttac tacgatttat aaaaaataag aagataatca ttattaaaga
    12421 aacgattaat ataatgttga atcctatagt atcacctaat tcgtttacac ctttgcctaa
    12481 tatcggagat acaaagcttt gtattaataa tgaaagagat gcccatatag taaatgaggt
    12541 tctgttgcaa agttgaattt atagtataat tttaacaaaa aggagtcttc tgtatgaact
    12601 atttcagata taaacaattt aacaaggatg ttatcactgt agctgttggc tactatctaa
    12661 gatatgcatt gagttatcgt gatatatctg aaatattaag ggaacgtggt gtaaacgttc
    12721 atcattcaac ggtctaccgt tgggttcaag aatatgcccc aattttatat caaatttgga
    12781 agaaaaagca taaaaaagct tattacaaat ggcgtattga tgagacgtac atcaaaataa
    12841 aaggaaaatg gagctattta tatcgtgcca ttgatgcaga gggacataca ttagatattt
    12901 ggttgcgtaa gcaacgagat aatcattcag catatgcgtt tatcaaacgt ctcattaaac
    12961 aatttggtaa acctcaaaag gtaattacag atcaggcacc ttcaacgaag gtagcaatgg
    13021 ttaaagtaat taaagctttt aaacttaaac ctgactgcca ttgtacatcg aaatatctga
    13081 ataacctcat tgagcaagat caccgtcata ttaaagtaag aaagacaagg tatcaaagta
    13141 tcaatacagc aaagaatact ttaaaaggta ttgagtgtat ttacgctcta tataaaaaga
    13201 accgcaggtc tcttcagatc tacggatttt cgccatgcca cgaaattagc atcatgctag
    13261 caagttaagc gaacactgac atgataaatt agtggttagc tatatttttt tactttgcaa
    13321 cagaacctta tttgaaatac tttagtttac ataatcgatg attaccagaa gttctcacta
    13381 tataaaagac actaaaattt tataactttt agtgcctaat caattatttt agctaccaaa
    13441 caaacgactc caaaacccct ttttaggttc cgtttcttca ctcttttctt ctatatcact
    13501 atcattgtca tttagattag ctggaacgtc ttcttttttt gcttcttcac cttgtgtact
    13561 ttcgtcgctt tgacttccag atatatcctt atattgaatc tctgtttctt tagtttcttc
    13621 ctgatctctg ttaatattta caggattagt agtatatgtt ttttcttgtg catcaacgtt
    13681 ttgtctagtg ttagtagatg tatcaaaggg ataattaagg tgtttttctt tttctaattg
    13741 attttctaat ttttgaatct ttttattact ctctaatgcc agaacttgct gattttcaag
    13801 caattttgat ctttcttgca actcttttct gtaaaattca attgttgttt catgacgact
    13861 ttcttgtttt tcaatttgat tatttaaagt atcaatctga tttttaagga tttctactat
    13921 ttgagcatcg cttttcgatt caaaattaat attatccgac cctttttttt gtttgtcttc
    13981 agtagtactt tcaaaaccat atttcttttt attttcattt actctttgaa tgattttttc
    14041 tatatcggta tcttctttaa taaacgaagt gttttcctgt tttattgtct ctatattcaa
    14101 tcgcttaata ttattaaaaa tcgtttgctt actaacccct aattcttcac tcaacttttt
    14161 cacacttttc atatcatcaa ccctttataa aatgtttata aacccttgat aaaatattat
    14221 aagcttataa tgcttaataa taaagtgatt aataaataaa aataaacatc catcaacctt
    14281 tttatcaagg gtttatgaaa ggtttat
//
