LOCUS       CP051968                3125 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain ER09615.3 plasmid pER09615.3A.1,
            complete sequence.
ACCESSION   CP051968
VERSION     CP051968.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN14669408
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.290803767661x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/23/2020 09:58:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,926
            CDSs (total)                      :: 2,844
            Genes (coding)                    :: 2,781
            CDSs (with protein)               :: 2,781
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 43 of 63
            Pseudo Genes (incomplete)         :: 23 of 63
            Pseudo Genes (internal stop)      :: 10 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER09615.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER09615.3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2017"
                     /collected_by="Mount Sinai Hospital"
     gene            join(3125,1..995)
                     /locus_tag="HIK68_14535"
     CDS             join(3125,1..995)
                     /locus_tag="HIK68_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QOB40066.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1578..1766
                     /locus_tag="HIK68_14540"
     CDS             1578..1766
                     /locus_tag="HIK68_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40067.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2093..2674)
                     /locus_tag="HIK68_14545"
     CDS             complement(2093..2674)
                     /locus_tag="HIK68_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOB40068.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1066..1230
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..995
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgcaatataa tactactaga agtataaccg aaaatcaaga taataaaacg ttaaaagata
       61 tgacgaaaag tgggaaacaa cgcccatgga gagaaaagaa aatagataat gtaagctatg
      121 cagatatact agaaatttta aaaatcaaaa aggcttttaa tgtaaaacaa tgtggtaata
      181 ttttagaatt taagccaact gatgaaggct atttgaagtt acataagaca tggttttgta
      241 aatcaaaatt atgtccggtt tgtaattgga gacgtgctat gaaaaatagt tatcaagctc
      301 aaaaagtgat tgaaaaagta attaaggaaa agccaaaagc acgttggttg tttttaacac
      361 tttcaacaaa aaatgcgata gatggagata ctttagaaca aagtttgaag catctaacta
      421 aagcatttga taggttgagt agatataaaa aggttaaaca aaatcttgtt ggatttatgc
      481 gttcaacaga agttaccgtt aataaaaatg acggtagtta taatcagcac atgcatgttt
      541 tgttatgtgt tgaaaatgca tattttagaa aaaaagagaa ttatataact caagaagaat
      601 gggttaattt atggcaaaga gcattacaag ttgactatcg acctgttgct aatgttaaag
      661 cgatcaaacc gaatagaaaa ggcgataaag acattgaatc ggcaatcaaa gagacctcaa
      721 aatattcggt taaatcatct gattttttaa ctgatgatga tgaaaaaaat caagaaattg
      781 taagtgattt agaaaaaggt ttgtatcgaa aacgtatgtt aagttatggt ggattgctta
      841 aacaaaaaca taaaatttta aacttagacg atgtcgaaga tggtaatttg attaatgcaa
      901 gtgatgaaga taaaacaaca gacgaagaag aaaaagcaca ttcaattacc gcaatttgga
      961 atttcgaaaa gcaaaattat tatttaagac attagtgttg actaatgtct tttttgttga
     1021 ttttttataa aaaagtactg tcttattttt gtgacaaatg ctgtatgtag tgtcacaaaa
     1081 ataagacaaa cgcaatatat tgtgtcacaa aaataagaca gtacagcttt gtatgatatc
     1141 actttaaaat accttaaaac ccctggaatt tctggctttg ccagacctat catttttgaa
     1201 tgatagcaaa ttctccttat gctcttacgg agtttttaga gaaaaattaa aaattctcga
     1261 tttttgataa aaaacgccct gcaggaattt agaaaaacat gtggaagttt taaaggattt
     1321 tatgctaatt tttaatttgc atgtaaatgg gcagtgttaa aaaattagtc actggttttg
     1381 ctgaaatttt tgtttgtgaa attaccatat atttatttgg ctcatatttg cgttttaaga
     1441 gcttgtatat atttttaagt taaaaaccta tatgataccc cctaaacctt taaaataccc
     1501 ccttaaaact caaaataaag gtatttataa tttgagtaag tcttgtgata aagtttttta
     1561 aaaaaggagt gtttattttg aataaaaaca cagtgttaga tgaaggttac gttatagcaa
     1621 ctattttaaa tgttttcttt ttacttggat taatttttat atctcatttg gaaaatttat
     1681 atatattaat tccgtatgtt attttgatgg gaataaatgc tatttatttg gttgttaagg
     1741 ttatgaattt caaaaagaat aattagaata tttgagggga ttttgaaacg agtttcttct
     1801 tgttttcaca ctgttttttt attcctattg gtgttgttgc ttactttttg tttttctata
     1861 aagatgatgc ttttgacgag atggaagaaa aatatgatta tcatgaagat aataaaaaat
     1921 agacgacgca tttatgccga gaaaatttat tgatgttgag aagaaccctt gagactacaa
     1981 cttgcagacg aatgtcgcat agcgtgagct attaagcgac cattcgacaa gttttgggat
     2041 tgttaagggt tccgaggctc aacgtcaata aagcaattgg aataaatagt atctaaagta
     2101 acccgtattt aagattacat tgtacatcct tcgaaaccat agtagctgcg attccagcta
     2161 ggtttccttt tatcccaatt ttagctaatt tttttgctac gccctttaag tttttggctt
     2221 taacatcttc aaaaactgct gtgattatgt ttccagttaa aagttctcca tatgagttta
     2281 aaacttcaga atagaaacag tcaccgctat ttttgtagtt cccaccaact gctctaggtg
     2341 atattgaact agttttcttt ttattctcta tgtcgttttt tagtttttgg aatccttcag
     2401 gaacatatcc atatcgagat ttggcatttt ctagattaac atctttagct gttccgtctt
     2461 tatctaaaat aataattttg ccatatatgt attttagttc tttttcaatt tctttttcgt
     2521 ttaaagtttc aacgttttta tctgcaatgt tatttttgtt ttgggcttga gcttctttga
     2581 aattaaaatc caagaccatt gaaagtatgg ttaaaatact taaagatact actaaaatct
     2641 ttcttagtga tatactgttt tgaattgcat tcattgtaaa tgccccttgt tttgttattt
     2701 aaatgaattc aatataatat taacatataa aaacaaagga gtttaatatg aataagtttt
     2761 tttacttttt gattatttta ttatctgtat ttttaatttt taattttgtt aattcgattt
     2821 tatttgataa tccttttgac tttttagaaa gttttattta ttcattggtt ttcacattaa
     2881 tttatttagg tttagagggt cataaaaaga agaaggaata ggttgttttt tgaaacgagt
     2941 gtgaacgagt ttcttcttgt cttgatacta tatagaaata actcgatttt atatatatag
     3001 ctgtaactgt tgatattaca gtgtttaaac gtgtttttgt gcgtgaaagg aaaatttgac
     3061 aataaaaaac cccagttata ttattaaggt gtcgaatctt aaataatact gggggtcttt
     3121 ttata
//
