LOCUS       CP051908               13163 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain ER11327.3 plasmid pER11327.3A.1,
            complete sequence.
ACCESSION   CP051908
VERSION     CP051908.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN14669443
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 161.565653280543x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/23/2020 10:16:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,838
            Genes (coding)                    :: 2,718
            CDSs (with protein)               :: 2,718
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 88 of 120
            Pseudo Genes (incomplete)         :: 26 of 120
            Pseudo Genes (internal stop)      :: 22 of 120
            Pseudo Genes (multiple problems)  :: 14 of 120
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER11327.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER11327.3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2017"
                     /collected_by="Mount Sinai Hospital"
     gene            221..964
                     /locus_tag="HIL03_00005"
     CDS             221..964
                     /locus_tag="HIL03_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QOA42385.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(1325..1903)
                     /locus_tag="HIL03_00010"
     CDS             complement(1325..1903)
                     /locus_tag="HIL03_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QOA42386.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(2167..2547)
                     /gene="blaI"
                     /locus_tag="HIL03_00015"
     ARG             complement(2171..2547)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(2537..4294)
                     /gene="blaR1"
                     /locus_tag="HIL03_00020"
     ARG             complement(2541..4294)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            4401..5246
                     /gene="blaZ"
                     /locus_tag="HIL03_00025"
     ARG             4402..5243
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5772..6191)
                     /locus_tag="HIL03_00030"
     CDS             complement(5772..6191)
                     /locus_tag="HIL03_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QOA42390.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            6315..7286
                     /locus_tag="HIL03_00035"
     CDS             6315..7286
                     /locus_tag="HIL03_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QOA42391.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(7458..7550)
                     /locus_tag="HIL03_00040"
     CDS             complement(7458..7550)
                     /locus_tag="HIL03_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QOA42392.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(7707..8054)
                     /locus_tag="HIL03_00045"
     CDS             complement(7707..8054)
                     /locus_tag="HIL03_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QOA42393.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(8073..8690)
                     /gene="cadD"
                     /locus_tag="HIL03_00050"
     ARG             complement(8077..8690)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9806..10000)
                     /locus_tag="HIL03_00055"
     CDS             complement(9806..10000)
                     /locus_tag="HIL03_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA42395.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            10110..10802
                     /locus_tag="HIL03_00060"
     CDS             10110..10802
                     /locus_tag="HIL03_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA42396.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            complement(10970..11119)
                     /locus_tag="HIL03_00065"
     CDS             complement(10970..11119)
                     /locus_tag="HIL03_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA42397.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(11666..11982)
                     /locus_tag="HIL03_00070"
                     /pseudo=""
     CDS             complement(11666..11982)
                     /locus_tag="HIL03_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(11975..12958)
                     /locus_tag="HIL03_00075"
     CDS             complement(11975..12958)
                     /locus_tag="HIL03_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QOA42398.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     ARG             4402..5246
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(11463..11610)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          222..964
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11669..11844)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(11976..12958)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attaagttta ttatagcact ttaaataaac ttataaaagg gaaaatatag ctttatcaag
       61 ggtttataaa cccttgataa atatcataaa cctttcataa acccttgata aaaaggttga
      121 tggatgttta tttttattta ttaatcactt tattattaag cattataagc ttataatatt
      181 ttatcaaggg tttataaaca ttttataaag ggttgatgat atgaaaagtg tgaaaaagtt
      241 gagtgaagaa ttaggggtta gtaagcaaac gatttttaat aatattaagc gattgaatat
      301 agagacaata aaacaggaaa acacttcgtt tattaaagaa gataccgata tagaaaaaat
      361 cattcaaaga gtaaatgaaa ataaaaagaa atatggtttt gaaagtacta ctgaagacaa
      421 acaaaaaaaa gggtcggata atattaattt tgaatcgaaa agcgatgctc aaatagtaga
      481 aatccttaaa aatcagattg atactttaaa taatcaaatt gaaaaacaag aaagtcgtca
      541 tgaaacaaca attgaatttt acagaaaaga gttgcaagaa agatcaaaat tgcttgaaaa
      601 tcagcaagtt ctggcattag agagtaataa aaagattcaa aaattagaaa atcaattaga
      661 aaaagaaaaa caccttaatt atccctttga tacatctact aacactagac aaaacgttga
      721 tgcacaagaa aaaacatata ctactaatcc tgtaaatatt aacagagatc aggaagaaac
      781 taaagaaaca gagattcaat ataaggatat atctggaagt caaagcgacg aaagtacaca
      841 aggtgaagaa gcaaaaaaag aagacgttcc agctaatcta aatgacaatg atagtgatat
      901 agaagaaaag agtgaagaaa cggaacctaa aaaggggttt tggagtcgtt tgtttggtag
      961 ctaaaataat tgattaggca ctaaaagtta taaaatttta gtgtctttta tatagtgaga
     1021 acttctggta atcatcgatt atgtaaacta aagtatttca aataattaac tgtattaata
     1081 ctctcatcat gattgcgtta taaacgttca ttagtgaaac aggtattata ttaaaaaagc
     1141 gtacgaattt gtacgctttt ttcttgtgtt attattaaaa tctcataaca ggaaaatatt
     1201 atgagatttt agacgctgtc agaacgtctc ttttacatcg agtgacattg aatcaaagtc
     1261 tttgtatgtt aatgatatat aaaattattt agtagattca ttatagtaag ctagattagt
     1321 tgctctactt gtttttattc tcttttgcct ctaatttatt gagataacga taaatagttg
     1381 tacgtgaaac ttgccactgc tctgcaattg ttttaattgg cgtaccatta tcataaagtg
     1441 tcttaagtaa gtttaaatct tgtttattta atttttcagg tctacctcca tatcggcctc
     1501 tagcacgtgc tgcaattcga ccggctgatg atcgctctaa aattaaatta cgttcaaact
     1561 ctgcaaatgc tgcgaataaa tgaaataata attgtcctgt agaacttgat ttatccatcg
     1621 ttatattttc ttctaaacta tgaaaactca cacctcgttc gttaagttca ttaactaacg
     1681 tgattaaatc ctccatgttc cgtcctagtc tatctaatct ccaaacaaca atcgtatctc
     1741 ctgacctcgc aaattcaatt gctttatcta aaccaggtcg cttacttttt gaaccactca
     1801 tatggtcatt aaatatcttt tcacaaccat atgtatttaa tcgatcctcc tgtaaattca
     1861 aattttgtaa tccagttgaa actcttgcat aacctatttt caaaatacag acctcctata
     1921 tacaccttta gtgtaccata acttgattac gaggtctttt gtgaacatag attttttgtt
     1981 gggtttttga acacataatt ctatgtaata cttacttatt tagacaaaaa acaagagtgt
     2041 acagaaaatg gttattttct ggacactctc atctttaata atatatttta aatgtcatta
     2101 acaaaactaa tttaataaga gtcaagtatg gtttacatat gtattatgtt tgaaacatac
     2161 ctctaattac tttttactaa tatcatttaa aatgtctcgc aattcttcaa tttctttgtt
     2221 atttaattct tcatttttcg caaaattcag cactaaactt ttcatatccc ctccatacag
     2281 tttattaaga aaggttttag cagttttcat tttaatatcg tcttctttaa tatttgatga
     2341 gtaaaaataa atattttctg atttgtatcg ttttataatc tcttttttat atagtcttgt
     2401 gattaatgtt ctaatcgttt tatcactaac ttctttatat ttttgaattt caactacaat
     2461 ttcattagct gatactgatt ttttatccca tattatattc ataacatccc attcagccat
     2521 agatatttca acttgcttat tggtcattta aaacacccat tccttttaat attttttcac
     2581 ttattaattc agcatttttc ccagacgggt ttccatctga taaatgtgta gcaaaataat
     2641 acttatcatg atttgtaatt acgtaaccta caaaccaccc attattatac ttcccgttta
     2701 ctatacctgt acctgttttc ccatacagtt catacttttc atttttctta atcaataatg
     2761 aagaagataa ttgattcttt gcttttttac taaaatggtt attttgttcc atcatatttt
     2821 taaaaactat tacttgttca agattagata ttttcaaact atcttccatc caatagcttt
     2881 tataacttcc caaattttca ttaccataat ttaattgctt gagttgagcc gcagtatagt
     2941 tctttggtat ttgattacta atacgttcga agtaccaatt aactgaattt tgcattgctg
     3001 tatttaaatc ttgttcctta ttccaagact cgaaaagata atgtttatga ttccatgaca
     3061 tacgtgaatt tttatcactt ataatatgac ggtcaagtcc aaacagtgct aaataaattt
     3121 tgtaagttga atcaggagaa tatctttttc tactttcttt ttcattataa atatagtatt
     3181 tgtctttctt catgctgtac ataacgaaag atccactatt tgaaccaaaa tttttacttt
     3241 catctaaaat ttggtaatcg ctttgtaagg gctttttata attataatca gttaaggatt
     3301 gccccattaa aaactggctt tgaattatca tgataaaaaa agtaaaaata caaataaaaa
     3361 ttagaattaa ttttgactgc tttttcagat cggcttcttt tatattaatt aatctacttt
     3421 tgagtaatga ttttttacca ttaaatgaat ggcttaatat atttttgttg gaaaatgggg
     3481 tttttaatac ggagtccata atagattccg cataagtttt aaattcattt ttgttaatat
     3541 tattaaggac aaatctatcg gcttctactt cattgtcatg aattatcttt ctttttacaa
     3601 tatgtactaa tggattgtaa ctcataataa tagagaagat attaaagata attaaatgta
     3661 aagtatctct atttttagca tgagcatatt catgcagtat tatatatttt agtcttttat
     3721 caattacact tttaaaatat gaactaggaa tcaaaataat atatttccca taccaaaaag
     3781 ttattggaga ttgaatagcc tctgcttttc gaatcacaat atttttttta tattgatggt
     3841 tgaaaagtat cgtatctatt ttattttttt cattttcgtt tagataaagt gactgtttct
     3901 ttaaatattt aagatataat aaggctttca aaaatttaaa acttaaaata ataactaaaa
     3961 ctatccaaat aactgtgcag atattatcaa ttgaatccca attaaactta tggatatctg
     4021 ttgtgaactc ttgaataggt ttggtggtat ttatgttatg gttcaaatcg tgtgacttac
     4081 tttcaactgt gggcgcttga ttattcacat tattaaattt tataaaagag aatttaattg
     4141 ggataaaagg aattaatcca gcaagcatag ttagatacca aactttataa tttaaactat
     4201 aattaaaata gcgttttaat atgtacctaa aaaatactaa caatagaaat ataaaacaaa
     4261 agcttactac gctcattatt agtaatttag ccattttgaa accttctttc aaatatttat
     4321 aataaacaat tgacatcaat attacaattg taatattatt gatttataaa aattacaact
     4381 gtaatatcgg agggtttatt ttgaaaaagt taatattttt aattgtaatt gctttagttt
     4441 taagtgcatg taattcaaac agttcacatg ccaaagagtt aaatgattta gaaaaaaaat
     4501 ataatgctca tattggtgtt tatgctttaa atactaaaag tggtaaggaa gtaaaattta
     4561 attcagataa gagatttgcc tatgcttcga cttcaaaagc gataaatagt gctattttgt
     4621 tagaacaagt accttataat aagttaaata aaaaagtaca tattaacaaa gatgatatag
     4681 ttgcttattc tcctatttta gaaaaatatg taggaaaaga tatcacttta aaagaactta
     4741 ttgaggcttc aatgacatat agtgataata cagcaaacaa taaaattata aaagaaatcg
     4801 gtggaatcaa aaaagttaaa caacgtctaa aagaactagg agataaagta acaaatccag
     4861 ttagatatga gatagaatta aattactatt caccaaagag caaaaaagat acttcaacgc
     4921 ctgctgcttt cggcaagact ttaaataaac ttatcgcaaa tggaaaatta agcaaaaaaa
     4981 ataaaaattt cttacttgat ttaatgttta ataataaaaa cggagacact ttaattaaag
     5041 atggtgttcc aaaagactat aaggttgctg ataaaagtgg tcaagcaata acatatgctt
     5101 ctagaaatga tgttgctttt gtttatccta agggccaatc tgaacctatt gttttagtca
     5161 tttttacgaa taaagacaat aaaagtgata agccaaatga taagttgata agtgaaaccg
     5221 ccaagagtgt aatgaaggaa ttttaatatt ctaaatgcat aataaatact gataacatct
     5281 tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata tcaaaaactg
     5341 attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa actagaaata
     5401 ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt gttcatcaat
     5461 cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat aaggttaaat
     5521 aattctcata tatcaagcaa agtgacaata gcattttaca agtatttttt attgttcgat
     5581 tagggtaaac ctaaataata cataaattat attacaatat gtttattata gaggctaaag
     5641 tatcttagaa atttggatag taggtttttc gggaataaaa gtatagttgg ctttaatata
     5701 taattaagct aagctatcat ttatatttac tcgattataa ttcgcacttc aactttttta
     5761 taacgtaaaa attaaaacgt agttttattt atctttagta atatcttttt aaacactaca
     5821 gtttcttcat tagttaaatt agcgaagtaa tgattattaa gttgataggc tttcttttct
     5881 aatgttggcc ataagtcttt tgctttattt gtactgttta ttagcacagt tcttctattt
     5941 tcattatcag tttgacgtgt tattagatcc tttttggaca tcttatctaa tattccagat
     6001 attgttgaag gttctaaatt taaagtatta ccaatcgctt taggtgtatt caaattacta
     6061 ttaatcaagc aattcaaaac accatattct gctggagtaa tattataggg acttaataaa
     6121 cttttaaaat gtttaaaaac tctattctga gaagtactca acaaataatt tatacaatta
     6181 tctaattcca caactaaaac ctctcttaaa tcattagtat tgactcgctt atcatatcat
     6241 gataagtttt gtaggaaaat agttttgcaa caaaaacata cttctacaaa acaattgtga
     6301 aagaaggcta ataaatgttc aatgcggtag tagtgagaaa aagtgcatca aatgttagtt
     6361 ataaagtaga aaaagttgat gaatctcatt tatcgaaggg tgatgtatta gtaaaagtgg
     6421 tatactcttc aataaattat aaggatatgc tagctttaca atataaaggc ggtgtaatta
     6481 gagattatcc tatgattcct ggtatagatt ttgcaggtat tgttgaatca agttcgaatg
     6541 ataagtttaa agaaggcgat aatgtattag ttactggtta tgatgttggc gtgacacata
     6601 caggtggatt ttcagaatat gcacaagtgc ctggagagtg gattgttcct attccccaag
     6661 aaatgtctat taaagagtct atggtatatg gcacagccgg ttttacggca gcattatcaa
     6721 tttttgaact agaaaaatat ggaatgaata tcaaatcaca acctgaaatt ttagtgtctg
     6781 gggctacagg tggtgtgggc agtgttgcta tacaaatttt acataaaatt ggatatgaaa
     6841 atatcactgc tatcgtaaga aaagaaaagc aaatagaaat cgcaaaaaaa ttaggtgcaa
     6901 aacaagtaat aataattgaa gatgataata aaccgcttaa aaaaggtatt tttgattatt
     6961 tcttagatac agtggggagt aatgtaacat tatacggttt aaaacgtcta aattatcaag
     7021 gtgttgctac tgtatgtgga aatgtagcag gaaattcact taacgctaat atattaccgt
     7081 ttattcttag aggtattaaa ttaattggca ttgattctgt atatgttgat cataatatcc
     7141 gtgaagatat atggagtaag ttagcaaatg aatggaatat aggaaattca cttctataca
     7201 atgaaattgg atttgaagaa ttctataaaa cgatagatca gcttttaaaa ggacaacatt
     7261 taggaagaac tattttaaaa gtataagtgt gagtattcat taatacattt actattaatt
     7321 caatcatttt gcatttctct actaaccagt gttataattt atataacgaa aatgattaac
     7381 aattaatgca ccaatcccca cactattgca gtagtgtggg gattttttcg atgctggctt
     7441 ataaagtcgc ctattaatta tttgttgcgt ttactaagcc aataagtata atacgcaaca
     7501 gcacagccac tgatgactgg tgctatgatg tgaacgaaaa tgattagcaa agtaatgcac
     7561 ctcctctctt cgtcaattga cgcctgagag ataggcgact ctattattat accatctgtg
     7621 aatatacaag catatctact tccgataagt cgtcttatgt tatataactt tgttttttat
     7681 agtatcaaga caagaagaaa ctcgtttcaa aaaccctcat gatttttaga gaaaaactct
     7741 cttatttcat cgtctttaat aaaataatat gccatctttc catctttata aaaatcaagt
     7801 acctcatttt tatataaaag ccttaaatga tgtgaagttg aagcaacact cattttcaat
     7861 atcaaagaaa tatcacaaac acacaactca tcttctttaa tcaaagataa tatgattttc
     7921 aatttcttct catcacaaat tttttctaaa atgttaagta atttcttaga tttatcatct
     7981 tctaaaaaac ttaaagcatt gttaacttta tcctcatgta cacagatcac atcacaagta
     8041 ttttcataac tcatataaaa tcacacccta aattaaaata taaatcctaa aattgtttga
     8101 atagtgtcat tttcaataat aataaataaa cctaaagcta tataaataat agccataatc
     8161 caacgactaa atttctcaac aatttctcca attcctggaa tattagctaa tttttgtgca
     8221 gtaaacacca agaagaaaat taaaattaaa aagacaaaca gagtaagtaa taaattagta
     8281 acacttaatg tcacaaaata cggaacaaat aaaccaatat tatcggcgcc acaacttgct
     8341 atcgtaacaa ttgcaaccgt accaactaat ttagacaatc ctttttcatt caattctttt
     8401 ttagctctct tttctccttc acaatcatca taaatagcca ctttaattcc aagataaatt
     8461 ggtattaaac ccaataaacc taatatccac ttttctggaa cataatttaa tacaaaagct
     8521 aaaaacaaac taactaatat taaaataata gaccctaaat attgaccaac ataaatatct
     8581 ctatattctt ttctagtctt tgctctagca aaaaatatta ataaaattac taataaatca
     8641 actgctgtag caatataaag aacagcagca gcaacaaccg tttgaatcat aatacacctc
     8701 attcaaaaat atttttgaat gtattataac ttatattttt aaataatcat aggtgcttta
     8761 ttccaattgc tttattgacg ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa
     8821 tggtcggctt aatagctcac gctatgccga cattcgtctg caagtttagt taagggttct
     8881 tctcaacatc aataaatttt ttcggcataa atgcgtgtct atgcgtagac atggagacac
     8941 ttcagaatta tggagtgatt acatgagttt aactattttt ctttattcag ttttaggaag
     9001 tttattagca aattatatct acgaaaaatc ttcaaaataa ataacttttt cggttctact
     9061 ttgtacactt tatctacctg gtagacctaa ttgttattgc tttacaggtc taaaattcgt
     9121 cgttataatc tagttaaatc ccgtaagggc gcacttatac gtcggataaa tcgacatgtg
     9181 caaaaacgta atcgaccttc gtcgattttt ttatttcaaa attaaagaaa cggcttgaga
     9241 tattccgagc agttataaaa attaatttgc gttgaatatt gctactacat tcttgaaaaa
     9301 gtgatgtaga tttatagccc ataacttttt tgaattacat cgacgatacg gcgtcgaaag
     9361 aaaaataaat agaccgtatg aaagaaaaag ctacctcact ttatctgggg tagcttttat
     9421 tttttatgcc cttattttga attttaagag ggcattttaa agatttaggg gtgattcata
     9481 tagttttata tctaaaactt atatatgctt ttaaaacgca aatatgagcc aaataaatat
     9541 atgctatttt caaaaacaaa aatttgagca aaaacaataa ctatttttag acactgccca
     9601 tttacatgca aattaaaaat tagcataaaa ttctttaaaa cttccccacg tttttctaaa
     9661 ttcctgtagg gcgtttttat caaaaatcaa gacaagaaga aactcgttca cactcgtttc
     9721 aaaaaaacaa gaaatctaat ctatacacct accgttttcc aaccccctca aagcatacaa
     9781 attaaaaaca aaattattca taatcttact ttcttttaac aaaaacataa aaaccataaa
     9841 tagacaaatt aactatcaaa ggcaataaac agtataaaaa aaccacgaat aaatcttcat
     9901 caacatctaa aataaaaaag aataaaacaa ttaacaaaat actaattaac aaacctaaaa
     9961 gtggcaaact ttttatatat ttttctaaat tttcattcat aaaatagact cctttcaaaa
    10021 atatgtttat aaatatatta aaataaatat tgattttata aagcgcttac ttatacaatt
    10081 aaaatatctt attatcttgg aggtatcaaa tgaaaattat aaaattattt tcaataattt
    10141 ccttgttcct actggtattt agcgcgaatt tttctaatgc ttacgcaaat gaagaacaaa
    10201 aaagttcttt actagaaaat caaaaagaaa aattgaaaag caaaaacatc gaagtatcac
    10261 aaatgaccaa gaaagaaata agttttctag aacaattgca aaataaaaat gaagatatgc
    10321 aaaaattcga aaagcaaatg aaaaaagaag gatttaaaaa aataaagcta gaagacaaaa
    10381 attctaaatt aaaatatcta gctacagaag caaatgataa aaatgcatat ggctacatag
    10441 ctaataacgc ttataaaaat gaaaaaacaa aagaaattgt agtttctgaa acaatatatg
    10501 ataactacaa caaaaaaatc acaaaattca ttgccgaaaa aagaagtatg actgaaaaca
    10561 aagagggaaa aatacttatt aacaaaaatt acgttgaata taaggctgaa aaagttgata
    10621 aaaacagacc ggtaacatat gcaggattca aatggaacgg caaagcattc gcttgtagta
    10681 tggctggact ttatgcatgt gctcactact gtggagtatg ggcaatcgta aatccaatag
    10741 ccggcggtgt atgtgaagca gtttgcggaa ctgcatttgc agctggatgc tctgtaggtt
    10801 gattttattc caattgtttt attgacgttg agcctcggaa cccttaacaa tcccaaaact
    10861 tgtcgaatgg tcggcttaat agctcacgct atgccgacat tcgtctgcaa gtttagttaa
    10921 gggttcttct caacatcaat aaattttttc ggcataaata cgatgttcct tatttatcct
    10981 cataaaaaac aatagctgaa atatataacc aattactgtg tgtttgaatt tctaatacat
    11041 gaatatcatt ctttaaaaac ctattaactt gtttatctaa ttgacgatga gtaaaagtag
    11101 cgaaagtttt aatcttcatg atatctctcc ttttataaaa atagcaatcc ttattacaat
    11161 attaaacact tttattttgg attttaagag gcattttgaa gacttagagg caaatcatat
    11221 aggttttgta cttaaaaatc tatacagtct cttaaaacgc aaatatgagc caaataaata
    11281 tatgctaatt tcacaaacaa aaatttcagc aaaatcagtg actaattttt tagatactgc
    11341 ccatttacat gcaaatttaa aaattagcat aaaatacttt aaaacttccc catgtttttc
    11401 taaattcctg cagggctttt cttatcaaaa acttagaaat tttaatttaa ctctaaaatc
    11461 tccgtaagag cataagggaa atttgctatc attcaaaaat gatagatctg gcaaggccag
    11521 acattccaag ggttttaagt tattttaaag tgatatcata caaagctgta gtgtcacact
    11581 tttgtgacac tacatacagc atttgtcaca aaaataagac agcacttttt tacagaaaaa
    11641 caataaaaaa agacatcagt tatcactaat gtcttaaata ataattttgc ttttcgaaat
    11701 tccaaattgc ggtaattgaa tgtgcttttt ttcttcgtct gtcgttttat cttcatcact
    11761 tgtattaatc aaattaccat cttcggcatc gtctaagttt aaaattttat gcttttgttt
    11821 aagcaatcca ccataactta acatacgttt tcgatacaaa cctttttcta aatcacttac
    11881 aatttcttga tttttttcgt cgtcatcagt taaaaagtca gatgatttaa cggaatattt
    11941 tgatgtctct tcgagtgtta taaaatcatt tgaattattg attaaagata ttatattgtt
    12001 tcatgttttt acggcgcata taaagggcat gtgtttcttc aagttcagtt agaattgttt
    12061 gcattaaata agcttgcatt gacttgattg tttccttttg gcctttaact cccctatcat
    12121 gtatagcttt tttaagtctt tttaaagaag ttaatatttc atcctcaatg tcctctaaca
    12181 tgtaatgcat atcttcatgc cttgaattaa atgatttttt tgctttaaga ataacagcct
    12241 ttatgatttc taaatctcta taatcatatt ttaataaata attagttagt tgttctggga
    12301 attcttgcaa tagcatttct ttatcatcaa aattatgtgt aaattgattt gaatgatttg
    12361 aatgattact aatttcattt ttgtacttta tatcattcaa gtcattcata tcattattct
    12421 cagtatcatt aaagtcaatg tcattaaagt cagtatcatt agtgttcgat tttcgaactt
    12481 ctggacgttc gattttcgaa cttctggacg ttcgattttc gaacttctgg acgttcgatt
    12541 ttcgaacttc cttctcgccg taaggttcaa catcgttttc ttctttttct attttgtaaa
    12601 tatcacgttc tgtgacaata ggtttcatta aatatagaat atttggttta tttaaaccac
    12661 gtcgtttttg aatgagtaga ccatcgtttt ctaacccttt ttttattttt gttaactttt
    12721 cttttttaca atttaagatt tccattagct tttcatttga ataaacaaag taaatgttac
    12781 catcttcatc aacccagcta tttttaatag ataaatttaa gcgatcacgt aagatagcat
    12841 aggctatttt catatcatta gttagatttt tataattttc actggtaaag aatactttag
    12901 ggatttgata aaatctttct ttgtaatttt cttgcactgt aaaatattga ttttgcatga
    12961 caaaaaacct ctttccatat tttagtttgg taagaggtaa taaaacgttt ttatttagct
    13021 ttacatttat ttaatgattt aggtgcttgc actaagatat tattcattat ataatacaag
    13081 tatcgaaaat tatataaata aacagtttct aaacgtttta ttaccaaacc ccgactatta
    13141 ccagtagttg gggtttttgt tat
//
