LOCUS       CP051836                2679 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_1997A plasmid pFLT_1997A-2,
            complete sequence.
ACCESSION   CP051836
VERSION     CP051836.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648851
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,217
            CDSs (with protein)               :: 5,217
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 94 of 214
            Pseudo Genes (incomplete)         :: 90 of 214
            Pseudo Genes (internal stop)      :: 60 of 214
            Pseudo Genes (multiple problems)  :: 23 of 214
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_1997A"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_1997A-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="1997"
                     /collected_by="USMARC"
     gene            join(2349..2679,1..74)
                     /locus_tag="HH261_27795"
     CDS             join(2349..2679,1..74)
                     /locus_tag="HH261_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI42684.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDE
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(475..732)
                     /locus_tag="HH261_27800"
     CDS             complement(475..732)
                     /locus_tag="HH261_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI42680.1"
                     /translation="MSKRQRLRRRARGRRFSIGSAPLTSIIKTDAQVRGGETRQDYKDT
                     RRFPLEAPSCALLFLPFGLPMSFRSYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            complement(920..1114)
                     /locus_tag="HH261_27805"
     CDS             complement(920..1114)
                     /locus_tag="HH261_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRI42681.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1398..1721
                     /locus_tag="HH261_27810"
     CDS             1398..1721
                     /locus_tag="HH261_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QRI42682.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTVEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGNREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            2080..2352
                     /locus_tag="HH261_27815"
     CDS             2080..2352
                     /locus_tag="HH261_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI42683.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     Typing          1153..1389
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          complement(379..886)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.441"
                     /coverage="95.0"
                     /evalue="2.2999999999999997e-156"
     Typing          1399..1718
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="1.59e-61"
ORIGIN
        1 gaacttctgc gaaggatatt gtgcaacaaa tgagattgat agagaaacgt tacgagcaag
       61 gtagtatgga ttaactggat gctgaacgtt tagcgaggtt acggcgtgcc aagggggggg
      121 ggcataatgt aaggatcttc ttgagatcct tgtcttgtcg tcgtagtctc ttgtctatga
      181 aacgaaaaaa ccacccggca aggtggtttt tcgaaggttc tcagagctac caactctttg
      241 agccgaggta actggcttgg aggagcgcag tcaccaaaac ttgttctttc agtttagcct
      301 taacaggcgc atgacttcaa ggctaactcc tctaattcgg ttaccagtgg ctgctgccag
      361 tggcgataag tcgtgtcttt ccgggttgga ttcaagtcga tagttaccgg ataaggcgca
      421 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
      481 ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taactgcgga atgacattgg
      541 taaaccgaaa ggcaggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcggttt ttatgatgct
      661 cgtcaggggg gcggagccta tggaaaaacg cctcccgcgc gcccttcttc tgagtctttg
      721 tcttttgctc acatgttctt tccggtttta tcccccgatt ctgtggataa ccgtattacc
      781 gcctttgagt gagctgacac cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
      841 agcgaggaag cggaagagcg ccttatgtga cattttctcc ttacgctcct ctatgccgtt
      901 ctgcatcctg tctggagcgt tatatcccgg taagattttc cgcttcaaag cgtgtctgta
      961 tgctgttctg gagttcttct gcgagttcgt gcagtttctc acacatggcg gcctgttcgt
     1021 cggcattgag tgcgtccagt ttttcgagca gcgtcaggct ctgacttttt atgaatcccg
     1081 ccatgttgag tacggcttgc tgctgcttgt tcatcttttc gttttctccg ttctgtctgt
     1141 catctgcgtt gtgtgattat atcgcgtacc acttttcgac tgttttgctg ccgctattct
     1201 gccgcttggc tttttgacgg gcatttctgt cagacaacac tgtcactgcc aaaaaactgc
     1261 cgtgcctttg tcggtaattc gagcttgctg acaggacagg atgtgcaatt gttataccgc
     1321 gcatacatgc acgctattac aattgccctg gtcagggctt tgccccgaca cccatgtcag
     1381 atacggagcc atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca
     1441 gtcgccctgt tcgcctgacg gtagaagaag accaggaaat cagaaaaagg gctgctgaat
     1501 gcggcaagac cgtttccggt tttttacggg cggcagctct cggtaagaaa gttaactcac
     1561 tgactgatga tcgggtactg aaagaagtta tgagactggg ggcgttacag aaaaaactct
     1621 ttatcgacgg caagcgtgtc gggaacaggg agtatgcgga ggtgctgatc gctattacgg
     1681 agtatcaccg tgccctgtta tccagactta tggcagatta gcttcccgga gagaaaactg
     1741 tcgaaaacag acggtatgaa caccgtaagc tcccaaagtg atcaccattc gctttcatgc
     1801 atagctatgc agcgagctga aaacgatcct gacgcatcct tcctgttttc ccggggtaaa
     1861 acatctcttt ttgcggtgtc tgcgtcagaa tcgcgttcag cgcgtttcag cggtgcgtac
     1921 aatactggta ttatggtaaa tgtaagattt tatatttatg tttttttgcg cacaaaacag
     1981 atatggatgt actctataaa gttacttgat caataaatta ttgagtgatg tgattgttcg
     2041 ctaataaata tatgagtata aggaatgctt ggagggaata tgagtgatgt taagtcagat
     2101 caaataatta aatatttgtc tgaaaagtgg ggagggagca aatgtccctt gtgtaaaaaa
     2161 gggcgttgga atgtttctga caaaattttt gaactaagag aatttcatga aggtaatgtt
     2221 gttattgggg caggtccgat agtcccaatt attcctgtaa cttgtgataa ttgcgggaat
     2281 gttgttcttg ttaatgccat tatctcaggg tgcttatctg ctgacaataa gcaagatgga
     2341 ggctcaaagt gagtggtggt aataatttta aaatgtctca ggactatgaa attgctcaac
     2401 caaaaaagaa aatggcttac cctattcttg ttgaagaatg ggatttttta atagggaaaa
     2461 tagaaagtat tagtgatgat gaaaatatat accatacgat tggttctata tttttaggtg
     2521 tggctggctc ggcattgata gcagcattaa ctttagattt gccatcagat gatggttata
     2581 tgccaaggca gatattaatt tcatggtacg tattttgggc aagccttatt tgtggagtgc
     2641 tttctatgtt ttttggtaga aagaataaaa agattaata
//
