LOCUS       CP051817                6078 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_2004G plasmid pFLT_2004G-2,
            complete sequence.
ACCESSION   CP051817
VERSION     CP051817.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648857
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:45:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,384
            Genes (coding)                    :: 5,179
            CDSs (with protein)               :: 5,179
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 92 of 205
            Pseudo Genes (incomplete)         :: 89 of 205
            Pseudo Genes (internal stop)      :: 54 of 205
            Pseudo Genes (multiple problems)  :: 23 of 205
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_2004G"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_2004G-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="2004"
                     /collected_by="USMARC"
     gene            8..199
                     /locus_tag="HH255_27050"
     CDS             8..199
                     /locus_tag="HH255_27050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI11360.1"
                     /translation="MLPLSAARNLGDLLPLSAARNLGDLLPLSAARNLGDLLPLSAARN
                     SGDLLPLSAARNLGHLLP"
                     /name="hypothetical protein"
     gene            253..768
                     /locus_tag="HH255_27055"
     CDS             253..768
                     /locus_tag="HH255_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="QRI11351.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(880..1590)
                     /locus_tag="HH255_27060"
     CDS             complement(880..1590)
                     /locus_tag="HH255_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI11352.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            2135..2392
                     /locus_tag="HH255_27065"
     CDS             2135..2392
                     /locus_tag="HH255_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI11353.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            2402..2683
                     /locus_tag="HH255_27070"
     CDS             2402..2683
                     /locus_tag="HH255_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRI11354.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     gene            complement(2996..3229)
                     /gene="mbeD"
                     /locus_tag="HH255_27075"
     CDS             complement(2996..3229)
                     /gene="mbeD"
                     /locus_tag="HH255_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QRI11355.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3236..3754)
                     /locus_tag="HH255_27080"
     CDS             complement(3236..3754)
                     /locus_tag="HH255_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QRI11356.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="mobilization protein"
     gene            complement(3732..4442)
                     /locus_tag="HH255_27085"
                     /pseudo=""
     CDS             complement(3732..4442)
                     /locus_tag="HH255_27085"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4432..4755)
                     /locus_tag="HH255_27090"
     CDS             complement(4432..4755)
                     /locus_tag="HH255_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QRI11357.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4820..5011
                     /locus_tag="HH255_27095"
     CDS             4820..5011
                     /locus_tag="HH255_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRI11358.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5652..5777
                     /locus_tag="HH255_27100"
     CDS             5652..5777
                     /locus_tag="HH255_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QRI11359.1"
                     /translation="MHEPPVQSDCCALSGNYPLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     Typing          complement(5090..5174)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          5474..5991
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2920..4442)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.762"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aggtgatttg ctacctttaa gtgcagctag aaatttaggt gatttgctac ctttaagtgc
       61 agctagaaat ttaggtgatt tgctaccttt aagtgcagct agaaatttag gtgatttgct
      121 acctttaagt gcagctagaa attcaggtga tttgctacct ttaagtgcag ctagaaattt
      181 aggtcattta ctaccttaaa gtaataaagt aaaaaaggcg actatacaca gtcgcctttt
      241 ttacgagaaa aaatgaaatt ttacacaata tcaagcagtt acataaaata cctgaaggac
      301 tttgatgaca aagttcccaa tagcgaagat cctacataca acaatcctaa ggctttcatt
      361 ggcatagtac tagagattga aggacataaa tatttagcac ctttaacatc gccaaaagca
      421 tggcatgcta atgtaaaaga gtcatcaccc gccttcttca aacttcatga aaacggtgtc
      481 cctgataatc agcttggatt gattaatctg aaatttatga ttccaataat cgaagctgaa
      541 gtgtctttac ttgatctgga tagcatgcct gatacgcctt acaaaagaat gctatataaa
      601 cagttacaat ttattcgtgt aaatgaagat aaaatatctg aaaaatcaaa actattaaga
      661 aaccttgcct tgcaaggcag aatgcaagga acatgcgact ttgccgtgtt agaagaaaaa
      721 tatcaacatt tcggtaaaaa acctgaagac atggaaatag acgattaacc attctttttt
      781 atggaattga aggtccaaaa tcaagcggtt ttggacctat tttttttgct ctcatccaat
      841 ataagaatga gatattctaa ttttgatttc gagaagcaat caaaaaggca agtcctcctc
      901 tgaaagatta agcacctctg atttcctttt gtaagtgaga gcgctgttac ttcccgctct
      961 aaagtagaag taacatgaat catgaaacat ttccaacggc ttgttttcta ttgatttttt
     1021 aacaatccta tcaagtaaac cgatatgaat aagataggta atagctttat ttagttcagg
     1081 tagtttgcat ttcagttttt tacatattgt ttttttcctg gcaagagtat actcatttct
     1141 attatctcga gcataaagaa ggtacatgta ggtttgtaaa gctagcaact caaatggata
     1201 tctgttgtgc atggattgaa atgcgcttat ttttccatct tcacctacaa ttcctttaaa
     1261 gggaagctta caccaaccat catcatgctt ctctaataca tctatggtat aaattttttt
     1321 acgtactgtc gttgataaat tttttatcaa ctcttttgca taaagaattt taagcccttc
     1381 gttaactaac gatcggctta atgatgctat ctcacatagg ttactaaaag ttagctcaac
     1441 agttctctgt cctgaatctg actctttgca gaaaaggcaa aatgttaaat aaatctttaa
     1501 cgcggcgatg gcttttgctg tatcaagatc gttgaattca gatattttga tacctatcca
     1561 tagtgaaggc attcttttcc agtacagcat aaatcacctc attcccagaa tttgaaaaat
     1621 gaaatagacc aaccccaata cagacataga tagcaccaga ttagttagca ccactgcgac
     1681 cttcgcaagc ctttcagcca aaaacacacc tttctcaaac attttttatc cctcctgata
     1741 gctttgtttt tttgattatc tcaggctgtg aaggcgttgt aacgccttca caaaaatcta
     1801 tctagaaaga taagataagg caaaagaaag atatctatat gttatataag atattttatc
     1861 ttgcttttgg tattcttaga aatcatatta tggttcatgg tgaaccgtaa atgcagtgct
     1921 gatgcttttc tggttacgtt aaatgttttc acattaggac tttagatcag aacaaacaaa
     1981 gcgttgtgcc ttgcaagcca ccctgcataa agcactaaat ttctttatct cattaaattc
     2041 accttgccaa aaggagcaat aatccctacc ttacaacttt aacaaaacta aaaatcttta
     2101 gcggacttag ccgccgagta tagagaaata tgccatgcct aaaaaaacat caaaaccaaa
     2161 cgatttaagc aacactatca acaacataaa gaaagaaatt aactcaggtt ttactgagtt
     2221 acttaatcgc gtagaagcct tagaggctag tgacgcacaa cacagcatgg cgatacgtga
     2281 tttacaaatt caggctagag ccgctcgcgg agacaagcgc atggatatag caagagactt
     2341 tggcctcagc gagggacgaa ttagccagat cgttaatgcg ggtcgtaact aagacagact
     2401 tatgaaaaat gaagacgact acaaaacagg atggacgact cagacaacaa acccggcaac
     2461 agggaaaaaa tgttctggag gcgcagccag aaacttaagg atatatcaag ccggtggagc
     2521 aaactccgtg cgggtaaaag ctgcaatcga gggtgtccag tcaatacagc caatcatcga
     2581 tatgcaacag tcacaaatcg agcaacaaca aactcaaata gcaatgctga ctcaatcttt
     2641 gtctcaggca atcaatgagc taacaaaaag caggaatcaa taggctataa aggatgttat
     2701 ttaccataac atccgttgcc cgcaccgccc cggacagctt cgccgcgaac ggggcggcag
     2761 tcagccaaaa ccaacaacaa atcacccgaa aagctaccgc gatcgcgcct gataaatttt
     2821 aaccgtatga atatctatgc aaccagatgg tgcagactac attagcccgt caaaatccac
     2881 tgaatctgcc attttttata gtttcgccat cacagcgaca acgaaggacc atccagcgtg
     2941 cgttcccgtt ccagggataa cgcatgttcg cgctcatgta tcaaccgttc ctctcttatc
     3001 tccagttctc gctgtataac tggctcaagc gttctgtccg ctcgctcaag tgctgcacct
     3061 gctgactcaa ctgcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa
     3121 acatcttctg caattccacg aaggcgctct cccattcgct cagccgctgc atatagtcct
     3181 gttgcagctg ctctaaggcg ttcagcaaat gtgtttccag ctctgtcact ctgtatcact
     3241 ccttcagatg tacccattcc ttcccctgaa aggaaatcac ctctgctgac ttcccgtaag
     3301 gaaggaccag aaatttcctg ttcccgtcct gcacaaactc cacgccccat gtcttcgcgt
     3361 tcagtttctg taatgtctct tcctgcttcc tgatatcttc caggttcgcc tgtatcctcc
     3421 cgccaagata ccagagcgtc ccgccactcg cggtaaacag gagaaagact atccccagta
     3481 acatcatgcc cgtattccct gccagcttta acacgtccct cctgtgctgc atcatcgcct
     3541 ctttcacccc ttcccggtgt ttttccagcg attcctccgt cgaggccgta aaccgggcta
     3601 tagcgccttt gattttcgtc tcgtttgagg tcacagcctc gcttacagat tcgccgagcc
     3661 tgctgaattc gtcgttcagc attttctctg tagattcggc tctctctttc agctttttct
     3721 cgaactccgc gcccgtccgt aaaagattgc tcataaaacg ctcctttcag tctgatattc
     3781 ctcccgccgt tcgggtctgc aatgctgata ctgcttcgcg tcaccctgac cacgtcaagc
     3841 cccgcctctg tgagcgcctg aatcacatcc tgacggcctt ttatctctcc gtaatggtaa
     3901 agcacctcca gacctcgtgt aacgctctca gcaagcgcct gtttcgtttc cggcaggtta
     3961 tcagggagtg tcagcgtcct gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg
     4021 tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc
     4081 tggaggcgtt ttccgctctg tagctccatg ttcggaatga caaaattcag ctcaagacgc
     4141 cccttgtcct ggtgctccac ccacagaatg ctgtactgat ttttttcgag accgggcatc
     4201 agtacgcgct caaagctctc catcactttt tcacgtcctc ccggcggcag ttccttctct
     4261 gcgaacgaca gaacaccgga cgtgtatttc ttcgcaaatg gcgtggcatc aataagctcc
     4321 cggacttctt ccgggttacc ctgaagtacc gttgcacctt cccggttacg ctccctcccc
     4381 agaaggtaat caaccggacc actgccacca ccttttcccc gggcatgaaa cttaactatc
     4441 atcccgcgct ccctgttccc tgacgacctg tcgtaagctg cgcaactctc tctcgatggc
     4501 catcagtgcg accaccacct gaacccggtc gccggaagac cactgcccgc tgttcacctt
     4561 cctcgccgtc tgattaaggt tattcccgat ggcagccagc tgacgcagta acggcggtgc
     4621 cagtgtcggc agcttcccgg aacgggcaac cggctcaccc aggcagaccc gccgcatcca
     4681 taccgccagt tgtttaccct cacagcgttc cagtaaccgg gcatgttcat catcagtaac
     4741 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     4801 cccttacacg gaggcatcag tgactaaaca ggaaaaaacc gcccttaaca tggcccgctt
     4861 tatcagaagc cagacattaa cgctgctgga gaaactcaac gaactggacg cggatgaaca
     4921 ggcagacatc tgtgaatctc ttcacgacca cgccgatgag ctttaccgca gctgcctcgc
     4981 acgcttcggg gatgacggtg aaaccctctg acacaagcag ctcccggaga cggtcacagc
     5041 ttgtctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag ggggtttcgg
     5101 ggcgaagccc tgaaccagtc acgtagcgat agcggagtgt atactggctt aaccatgcgg
     5161 catcagtgca gattgtatga aaagtgcgcc atgccgggtg tgaaatgccg cacagatgcg
     5221 taaggagaaa atgcagcatc agacgctctt ccgcttcctc gctcactgac tcgctacgct
     5281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa aacggtaatg cggttatcca
     5341 cagaatcagg ggataacacc aaaagaaaca tgtgagcaaa aaacaagaac ccggaaaagg
     5401 ccacgccgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaaccgac
     5461 gctcaagtca gaggtggcga aacccgacag gacttaaaga taccaggcgt ttccccctgg
     5521 tggctccctc gtgcgctctc ctgttcctgc cattcggttt accggtgtca ctccgctgtt
     5581 atggccgcgt ttatctcatt ccacgcctga cactcggttc cgggtaggca gttcgctcca
     5641 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact
     5701 atccacttga gtccaaaccg gaaagacacg acaaagcgcc actggcagca gccattggta
     5761 acttggttcg cagatgattt gttagctaaa cacgaggttg ctcttgaagt gtgcgcctca
     5821 gtacggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
     5881 gtccagaaac ttgccggttt actgaacctt cgaaaaaacc accttgccgg gtggtttttc
     5941 cgttttcaga ccaagagatt acgcgcagaa aaagatgatc tcaagaagat ccttttgatt
     6001 agtgccgtca tttgactgaa catcccatgc taagcaccta gttgtaagcc caagcgaaga
     6061 tcatgtatac ttagttca
//
