LOCUS       CP051812                6078 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_2006A plasmid pFLT_2006A-2,
            complete sequence.
ACCESSION   CP051812
VERSION     CP051812.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648859
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:59:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,430
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,101
            CDSs (with protein)               :: 5,101
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 85 of 199
            Pseudo Genes (internal stop)      :: 54 of 199
            Pseudo Genes (multiple problems)  :: 23 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_2006A"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_2006A-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="2006"
                     /collected_by="USMARC"
     gene            246..761
                     /locus_tag="HH253_26620"
     CDS             246..761
                     /locus_tag="HH253_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="QRH97895.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(873..1583)
                     /locus_tag="HH253_26625"
     CDS             complement(873..1583)
                     /locus_tag="HH253_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH97896.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            2128..2385
                     /locus_tag="HH253_26630"
     CDS             2128..2385
                     /locus_tag="HH253_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH97897.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            2395..2676
                     /locus_tag="HH253_26635"
     CDS             2395..2676
                     /locus_tag="HH253_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH97898.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     gene            complement(2989..3222)
                     /gene="mbeD"
                     /locus_tag="HH253_26640"
     CDS             complement(2989..3222)
                     /gene="mbeD"
                     /locus_tag="HH253_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QRH97899.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3229..3747)
                     /locus_tag="HH253_26645"
     CDS             complement(3229..3747)
                     /locus_tag="HH253_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QRH97900.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="mobilization protein"
     gene            complement(3725..4435)
                     /locus_tag="HH253_26650"
                     /pseudo=""
     CDS             complement(3725..4435)
                     /locus_tag="HH253_26650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4425..4748)
                     /locus_tag="HH253_26655"
     CDS             complement(4425..4748)
                     /locus_tag="HH253_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QRH97901.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4813..5004
                     /locus_tag="HH253_26660"
     CDS             4813..5004
                     /locus_tag="HH253_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRH97902.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5645..5770
                     /locus_tag="HH253_26665"
     CDS             5645..5770
                     /locus_tag="HH253_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QRH97903.1"
                     /translation="MHEPPVQSDCCALSGNYPLESKPERHDKAPLAAAIGNLVRR"
                     /name="replication initiation protein"
     Typing          complement(5083..5167)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          5467..5984
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2913..4435)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.762"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttgctacctt taagtgcagc tagaaattta ggtgatttgc tacctttaag tgcagctaga
       61 aatttaggtg atttgctacc tttaagtgca gctagaaatt taggtgattt gctaccttta
      121 agtgcagcta gaaattcagg tgatttgcta cctttaagtg cagctagaaa tttaggtcat
      181 ttactacctt aaagtaataa agtaaaaaag gcgactatac acagtcgcct tttttacgag
      241 aaaaaatgaa attttacaca atatcaagca gttacataaa atacctgaag gactttgatg
      301 acaaagttcc caatagcgaa gatcctacat acaacaatcc taaggctttc attggcatag
      361 tactagagat tgaaggacat aaatatttag cacctttaac atcgccaaaa gcatggcatg
      421 ctaatgtaaa agagtcatca cccgccttct tcaaacttca tgaaaacggt gtccctgata
      481 atcagcttgg attgattaat ctgaaattta tgattccaat aatcgaagct gaagtgtctt
      541 tacttgatct ggatagcatg cctgatacgc cttacaaaag aatgctatat aaacagttac
      601 aatttattcg tgtaaatgaa gataaaatat ctgaaaaatc aaaactatta agaaaccttg
      661 ccttgcaagg cagaatgcaa ggaacatgcg actttgccgt gttagaagaa aaatatcaac
      721 atttcggtaa aaaacctgaa gacatggaaa tagacgatta accattcttt tttatggaat
      781 tgaaggtcca aaatcaagcg gttttggacc tatttttttt gctctcatcc aatataagaa
      841 tgagatattc taattttgat ttcgagaagc aatcaaaaag gcaagtcctc ctctgaaaga
      901 ttaagcacct ctgatttcct tttgtaagtg agagcgctgt tacttcccgc tctaaagtag
      961 aagtaacatg aatcatgaaa catttccaac ggcttgtttt ctattgattt tttaacaatc
     1021 ctatcaagta aaccgatatg aataagatag gtaatagctt tatttagttc aggtagtttg
     1081 catttcagtt ttttacatat tgtttttttc ctggcaagag tatactcatt tctattatct
     1141 cgagcataaa gaaggtacat gtaggtttgt aaagctagca actcaaatgg atatctgttg
     1201 tgcatggatt gaaatgcact tatttttcca tcttcaccta caattccttt aaagggaagc
     1261 ttacaccaac catcatcatg cttctctaat acatctatgg tataaatttt tttacgtact
     1321 gtcgttgata aattttttat caactctttt gcataaagaa ttttaagccc ttcgttaact
     1381 aacgatcggc ttaatgatgc tatctcacat aggttactaa aagttagctc aacagttctc
     1441 tgtcctgaat ctgactcttt gcagaaaagg caaaatgtta aataaatctt taacgcggcg
     1501 atggcttttg ctgtatcaag atcgttgaat tcagatattt tgatacctat ccatagtgaa
     1561 ggcattcttt tccagtacag cataaatcac ctcattccca gaatttgaaa aatgaaatag
     1621 accaacccca atacagacat agatagcacc agattagtta gcaccactgc gaccttcgca
     1681 agcctttcag ccaaaaacac acctttctca aacatttttt atccctcctg atagctttgt
     1741 ttttttgatt atctcaggct gtgaaggcgt tgtaacgcct tcacaaaaat ctatctagaa
     1801 agataagata aggcaaaaga aagatatcta tatgttatat aagatatttt atcttgcttt
     1861 tggtattctt agaaatcata ttatggttca tggtgaaccg taaatgcagt gctgatgctt
     1921 ttctggttac gttaaatgtt ttcacattag gactttagat cagaacaaac aaagcgttgt
     1981 gccttgcaag ccaccctgca taaagcacta aatttcttta tctcattaaa ttcaccttgc
     2041 caaaaggagc aataatccct accttacaac tttaacaaaa ctaaaaatct ttagcggact
     2101 tagccgccga gtatagagaa atatgccatg cctaaaaaaa catcaaaacc aaacgattta
     2161 agcaacacta tcaacaacat aaagaaagaa attaactcag gttttactga gttacttaat
     2221 cgcgtagaag ccttagaggc tagtgacgca caacacagca tggcgatacg tgatttacaa
     2281 attcaggcta gagccgctcg cggagacaag cgcatggata tagcaagaga ctttggcctc
     2341 agcgagggac gaattagcca gatcgttaat gcgggtcgta actaagacag acttatgaaa
     2401 aatgaagacg actacaaaac aggatggacg actcagacaa caaacccggc aacagggaaa
     2461 aaatgttctg gaggcgcagc cagaaactta aggatatatc aagccggtgg agcaaactcc
     2521 gtgcgggtaa aagctgcaat cgagggtgtc cagtcaatac agccaatcat cgatatgcaa
     2581 cagtcacaaa tcgagcaaca acaaactcaa atagcaatgc tgactcaatc tttgtctcag
     2641 gcaatcaatg agctaacaaa aagcaggaat caataggcta taaaggatgt tatttaccat
     2701 aacatccgtt gcccgcaccg ccccggacag cttcgccgcg aacggggcgg cagtcagcca
     2761 aaaccaacaa caaatcaccc gaaaagctac cgcgatcgcg cctgataaat tttaaccgta
     2821 tgaatatcta tgcaaccaga tggtgcagac tacattagcc cgtcaaaatc cactgaatct
     2881 gccatttttt atagtttcgc catcacagcg acaacgaagg accatccagc gtgcgttccc
     2941 gttccaggga taacgcatgt tcgcgctcat gtatcaaccg ttcctctctt atctccagtt
     3001 ctcgctgtat aactggctca agcgttctgt ccgctcgctc aagtgctgca cctgctgact
     3061 caactgcatg acccgctcgt tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt
     3121 ctgcaattcc acgaaggcgc tctcccattc gctcagccgc tgcatatagt cctgttgcag
     3181 ctgctctaag gcgttcagca aatgtgtttc cagctctgtc actctgtatc actccttcag
     3241 atgtacccat tccttcccct gaaaggaaat cacctctgct gacttcccgt aaggaaggac
     3301 cagaaatttc ctgttcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt
     3361 ctgtaatgtc tcttcctgct tcctgatatc ttccaggttc gcctgtatcc tcccgccaag
     3421 ataccagagc gtcccgccac tcgcggtaaa caggagaaag actatcccca gtaacatcat
     3481 gcccgtattc cctgccagct ttaacacgtc cctcctgtgc tgcatcatcg cctctttcac
     3541 cccttcccgg tgtttttcca gcgattcctc cgtcgaggcc gtaaaccggg ctatagcgcc
     3601 tttgattttc gtctcgtttg aggtcacagc ctcgcttaca gattcgccga gcctgctgaa
     3661 ttcgtcgttc agcattttct ctgtagattc ggctctctct ttcagctttt tctcgaactc
     3721 cgcgcccgtc cgtaaaagat tgctcataaa acgctccttt cagtctgata ttcctcccgc
     3781 cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct gaccacgtca agccccgcct
     3841 ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc tccgtaatgg taaagcacct
     3901 ccagacctcg tgtaacgctc tcagcaagcg cctgtttcgt ttccggcagg ttatcaggga
     3961 gtgtcagcgt cctgcggttc tccggggcgt tggggtcatg cagcccgtaa tggtgattca
     4021 ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac ggctggaggc
     4081 gttttccgct ctgtagctcc atgttcggaa tgacaaaatt cagctcaaga cgccccttgt
     4141 cctggtgctc cacccacaga atgctgtact gatttttttc gagaccgggc atcagtacgc
     4201 gctcaaagct ctccatcact ttttcacgtc ctcccggcgg cagttccttc tctgcgaacg
     4261 acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc atcaataagc tcccggactt
     4321 cttccgggtt accctgaagt accgttgcac cttcccggtt acgctccctc cccagaaggt
     4381 aatcaaccgg accactgcca ccaccttttc cccgggcatg aaacttaact atcatcccgc
     4441 gctccctgtt ccctgacgac ctgtcgtaag ctgcgcaact ctctctcgat ggccatcagt
     4501 gcgaccacca cctgaacccg gtcgccggaa gaccactgcc cgctgttcac cttcctcgcc
     4561 gtctgattaa ggttattccc gatggcagcc agctgacgca gtaacggcgg tgccagtgtc
     4621 ggcagcttcc cggaacgggc aaccggctca cccaggcaga cccgccgcat ccataccgcc
     4681 agttgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     4741 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     4801 acggaggcat cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     4861 agccagacat taacgctgct ggagaaactc aacgaactgg acgcggatga acaggcagac
     4921 atctgtgaat ctcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgcttc
     4981 ggggatgacg gtgaaaccct ctgacacaag cagctcccgg agacggtcac agcttgtctg
     5041 tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagggggttt cggggcgaag
     5101 ccctgaacca gtcacgtagc gatagcggag tgtatactgg cttaaccatg cggcatcagt
     5161 gcagattgta tgaaaagtgc gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag
     5221 aaaatgcagc atcagacgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt
     5281 tcggctgcgg cgagcggtat cagctcactc aaaaacggta atgcggttat ccacagaatc
     5341 aggggataac accaaaagaa acatgtgagc aaaaaacaag aacccggaaa aggccacgcc
     5401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaacc gacgctcaag
     5461 tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc tggtggctcc
     5521 ctcgtgcgct ctcctgttcc tgccattcgg tttaccggtg tcactccgct gttatggccg
     5581 cgtttatctc attccacgcc tgacactcgg ttccgggtag gcagttcgct ccaagctgga
     5641 ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta actatccact
     5701 tgagtccaaa ccggaaagac acgacaaagc gccactggca gcagccattg gtaacttggt
     5761 tcgcagatga tttgttagct aaacacgagg ttgctcttga agtgtgcgcc tcagtacggc
     5821 tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggtccaga
     5881 aacttgccgg tttactgaac cttcgaaaaa accaccttgc cgggtggttt ttccgttttc
     5941 agaccaagag attacgcgca gaaaaagatg atctcaagaa gatccttttg attagtgccg
     6001 tcatttgact gaacatccca tgctaagcac ctagttgtaa gcccaagcga agatcatgta
     6061 tacttagttc aaggtgat
//
