LOCUS       CP051793                2679 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_2011B plasmid pFLT_2011B-2,
            complete sequence.
ACCESSION   CP051793
VERSION     CP051793.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648868
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 284x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:53:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,276
            CDSs (with protein)               :: 5,276
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 100 of 226
            Pseudo Genes (incomplete)         :: 97 of 226
            Pseudo Genes (internal stop)      :: 59 of 226
            Pseudo Genes (multiple problems)  :: 23 of 226
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_2011B"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_2011B-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="2011"
                     /collected_by="USMARC"
     gene            join(2627..2679,1..352)
                     /locus_tag="HH244_27430"
     CDS             join(2627..2679,1..352)
                     /locus_tag="HH244_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH50972.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDE
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(753..1010)
                     /locus_tag="HH244_27435"
     CDS             complement(753..1010)
                     /locus_tag="HH244_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH50973.1"
                     /translation="MSKRQRLRRRARGRRFSIGSAPLTSIIKTDAQVRGGETRQDYKDT
                     RRFPLEAPSCALLFLPFGLPMSFRSYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            complement(1198..1392)
                     /locus_tag="HH244_27440"
     CDS             complement(1198..1392)
                     /locus_tag="HH244_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRH50974.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1676..1999
                     /locus_tag="HH244_27445"
     CDS             1676..1999
                     /locus_tag="HH244_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QRH50975.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTVEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGNREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            2358..2630
                     /locus_tag="HH244_27450"
     CDS             2358..2630
                     /locus_tag="HH244_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH50976.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     Typing          1431..1667
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          complement(657..1164)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.441"
                     /coverage="95.0"
                     /evalue="2.2999999999999997e-156"
     Typing          1677..1996
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="1.59e-61"
ORIGIN
        1 aaaaaagaaa atggcttacc ctattcttgt tgaagaatgg gattttttaa tagggaaaat
       61 agaaagtatt agtgatgatg aaaatatata ccatacgatt ggttctatat ttttaggtgt
      121 ggctggctcg gcattgatag cagcattaac tttagatttg ccatcagatg atggttatat
      181 gccaaggcag atattaattt catggtacgt attttgggca agccttattt gtggagtgct
      241 ttctatgttt tttggtagaa agaataaaaa gattaataga acttctgcga aggatattgt
      301 gcaacaaatg agattgatag agaaacgtta cgagcaaggt agtatggatt aactggatgc
      361 tgaacgttta gcgaggttac ggcgtgccaa gggggggggg cataatgtaa ggatcttctt
      421 gagatccttg tcttgtcgtc gtagtctctt gtctatgaaa cgaaaaaacc acccggcaag
      481 gtggtttttc gaaggttctc agagctacca actctttgag ccgaggtaac tggcttggag
      541 gagcgcagtc accaaaactt gttctttcag tttagcctta acaggcgcat gacttcaagg
      601 ctaactcctc taattcggtt accagtggct gctgccagtg gcgataagtc gtgtctttcc
      661 gggttggatt caagtcgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      721 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      781 atgagataaa cgcggccata actgcggaat gacattggta aaccgaaagg caggaacagg
      841 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      901 tcgccacctc tgacttgagc gtcggttttt atgatgctcg tcaggggggc ggagcctatg
      961 gaaaaacgcc tcccgcgcgc ccttcttctg agtctttgtc ttttgctcac atgttctttc
     1021 cggttttatc ccccgattct gtggataacc gtattaccgc ctttgagtga gctgacaccg
     1081 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc
     1141 ttatgtgaca ttttctcctt acgctcctct atgccgttct gcatcctgtc tggagcgtta
     1201 tatcccggta agattttccg cttcaaagcg tgtctgtatg ctgttctgga gttcttctgc
     1261 gagttcgtgc agtttctcac acatggcggc ctgttcgtcg gcattgagtg cgtccagttt
     1321 ttcgagcagc gtcaggctct gactttttat gaatcccgcc atgttgagta cggcttgctg
     1381 ctgcttgttc atcttttcgt tttctccgtt ctgtctgtca tctgcgttgt gtgattatat
     1441 cgcgtaccac ttttcgactg ttttgctgcc gctattctgc cgcttggctt tttgacgggc
     1501 atttctgtca gacaacactg tcactgccaa aaaactgccg tgcctttgtc ggtaattcga
     1561 gcttgctgac aggacaggat gtgcaattgt tataccgcgc atacatgcac gctattacaa
     1621 ttgccctggt cagggctttg ccccgacacc catgtcagat acggagccat gttttatgac
     1681 aaaacgaagt ggaagtaata cgcgcaggcg ggctatcagt cgccctgttc gcctgacggt
     1741 agaagaagac caggaaatca gaaaaagggc tgctgaatgc ggcaagaccg tttccggttt
     1801 tttacgggcg gcagctctcg gtaagaaagt taactcactg actgatgatc gggtactgaa
     1861 agaagttatg agactggggg cgttacagaa aaaactcttt atcgacggca agcgtgtcgg
     1921 gaacagggag tatgcggagg tgctgatcgc tattacggag tatcaccgtg ccctgttatc
     1981 cagacttatg gcagattagc ttcccggaga gaaaactgtc gaaaacagac ggtatgaaca
     2041 ccgtaagctc ccaaagtgat caccattcgc tttcatgcat agctatgcag cgagctgaaa
     2101 acgatcctga cgcatccttc ctgttttccc ggggtaaaac atctcttttt gcggtgtctg
     2161 cgtcagaatc gcgttcagcg cgtttcagcg gtgcgtacaa tactggtatt atggtaaatg
     2221 taagatttta tatttatgtt tttttgcgca caaaacagat atggatgtac tctataaagt
     2281 tacttgatca ataaattatt gagtgatgtg attgttcgct aataaatata tgagtataag
     2341 gaatgcttgg agggaatatg agtgatgtta agtcagatca aataattaaa tatttgtctg
     2401 aaaagtgggg agggagcaaa tgtcccttgt gtaaaaaagg gcgttggaat gtttctgaca
     2461 aaatttttga actaagagaa tttcatgaag gtaatgttgt tattggggca ggtccgatag
     2521 tcccaattat tcctgtaact tgtgataatt gcgggaatgt tgttcttgtt aatgccatta
     2581 tctcagggtg cttatctgct gacaataagc aagatggagg ctcaaagtga gtggtggtaa
     2641 taattttaaa atgtctcagg actatgaaat tgctcaacc
//
