LOCUS       CP051787                2680 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_2012I plasmid pFLT_2012I-2,
            complete sequence.
ACCESSION   CP051787
VERSION     CP051787.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648870
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:52:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,636
            CDSs (total)                      :: 5,503
            Genes (coding)                    :: 5,275
            CDSs (with protein)               :: 5,275
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 99 of 228
            Pseudo Genes (incomplete)         :: 98 of 228
            Pseudo Genes (internal stop)      :: 61 of 228
            Pseudo Genes (multiple problems)  :: 23 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_2012I"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_2012I-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="2012"
                     /collected_by="USMARC"
     gene            join(2467..2680,1..191)
                     /locus_tag="HH242_27450"
     CDS             join(2467..2680,1..191)
                     /locus_tag="HH242_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH40570.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDE
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(593..850)
                     /locus_tag="HH242_27455"
     CDS             complement(593..850)
                     /locus_tag="HH242_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH40571.1"
                     /translation="MSKRQRLRRRARGRRFSIGSAPLTSIIKTDAQVRGGETRQDYKDT
                     RRFPLEAPSCALLFLPFGLPMSFRSYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            complement(1038..1232)
                     /locus_tag="HH242_27460"
     CDS             complement(1038..1232)
                     /locus_tag="HH242_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRH40572.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1516..1839
                     /locus_tag="HH242_27465"
     CDS             1516..1839
                     /locus_tag="HH242_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QRH40573.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTVEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGNREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            2198..2470
                     /locus_tag="HH242_27470"
     CDS             2198..2470
                     /locus_tag="HH242_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH40574.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     Typing          1271..1507
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          complement(497..1004)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.441"
                     /coverage="95.0"
                     /evalue="2.2999999999999997e-156"
     Typing          1517..1836
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="1.59e-61"
ORIGIN
        1 catcagatga tggttatatg ccaaggcaga tattaatttc atggtacgta ttttgggcaa
       61 gccttatttg tggagtgctt tctatgtttt ttggtagaaa gaataaaaag attaatagaa
      121 cttctgcgaa ggatattgtg caacaaatga gattgataga gaaacgttac gagcaaggta
      181 gtatggatta actggatgct gaacgtttag cgaggttacg gcgtgccaag gggggggggg
      241 cataatgtaa ggatcttctt gagatccttg tcttgtcgtc gtagtctctt gtctatgaaa
      301 cgaaaaaacc acccggcaag gtggtttttc gaaggttctc agagctacca actctttgag
      361 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gttctttcag tttagcctta
      421 acaggcgcat gacttcaagg ctaactcctc taattcggtt accagtggct gctgccagtg
      481 gcgataagtc gtgtctttcc gggttggatt caagtcgata gttaccggat aaggcgcagc
      541 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
      601 aactgagtgt caggcgtgga atgagataaa cgcggccata actgcggaat gacattggta
      661 aaccgaaagg caggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt atgatgctcg
      781 tcaggggggc ggagcctatg gaaaaacgcc tcccgcgcgc ccttcttctg agtctttgtc
      841 ttttgctcac atgttctttc cggttttatc ccccgattct gtggataacc gtattaccgc
      901 ctttgagtga gctgacaccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
      961 cgaggaagcg gaagagcgcc ttatgtgaca ttttctcctt acgctcctct atgccgttct
     1021 gcatcctgtc tggagcgtta tatcccggta agattttccg cttcaaagcg tgtctgtatg
     1081 ctgttctgga gttcttctgc gagttcgtgc agtttctcac acatggcggc ctgttcgtcg
     1141 gcattgagtg cgtccagttt ttcgagcagc gtcaggctct gactttttat gaatcccgcc
     1201 atgttgagta cggcttgctg ctgcttgttc atcttttcgt tttctccgtt ctgtctgtca
     1261 tctgcgttgt gtgattatat cgcgtaccac ttttcgactg ttttgctgcc gctattctgc
     1321 cgcttggctt tttgacgggc atttctgtca gacaacactg tcactgccaa aaaactgccg
     1381 tgcctttgtc ggtaattcga gcttgctgac aggacaggat gtgcaattgt tataccgcgc
     1441 atacatgcac gctattacaa ttgccctggt cagggctttg ccccgacacc catgtcagat
     1501 acggagccat gttttatgac aaaacgaagt ggaagtaata cgcgcaggcg ggctatcagt
     1561 cgccctgttc gcctgacggt agaagaagac caggaaatca gaaaaagggc tgctgaatgc
     1621 ggcaagaccg tttccggttt tttacgggcg gcagctctcg gtaagaaagt taactcactg
     1681 actgatgatc gggtactgaa agaagttatg agactggggg cgttacagaa aaaactcttt
     1741 atcgacggca agcgtgtcgg gaacagggag tatgcggagg tgctgatcgc tattacggag
     1801 tatcaccgtg ccctgttatc cagacttatg gcagattagc ttcccggaga gaaaactgtc
     1861 gaaaacagac ggtatgaaca ccgtaagctc ccaaagtgat caccattcgc tttcatgcat
     1921 agctatgcag cgagctgaaa acgatcctga cgcatccttc ctgttttccc ggggtaaaac
     1981 atctcttttt gcggtgtctg cgtcagaatc gcgttcagcg cgtttcagcg gtgcgtacaa
     2041 tactggtatt atggtaaatg taagatttta tatttatgtt tttttgcgca caaaacagat
     2101 atggatgtac tctataaagt tacttgatca ataaattatt gagtgatgtg attgttcgct
     2161 aataaatata tgagtataag gaatgcttgg agggaatatg agtgatgtta agtcagatca
     2221 aataattaaa tatttgtctg aaaagtgggg agggagcaaa tgtcccttgt gtaaaaaagg
     2281 gcgttggaat gtttctgaca aaatttttga actaagagaa tttcatgaag gtaatgttgt
     2341 tattggggca ggtccgatag tcccaattat tcctgtaact tgtgataatt gcgggaatgt
     2401 tgttcttgtt aatgccatta tctcagggtg cttatctgct gacaataagc aagatggagg
     2461 ctcaaagtga gtggtggtaa taattttaaa atgtctcagg actatgaaat tgctcaacca
     2521 aaaaagaaaa tggcttaccc tattcttgtt gaagaatggg attttttaat agggaaaata
     2581 gaaagtatta gtgatgatga aaatatatac catacgattg gttctatatt tttaggtgtg
     2641 gctggctcgg cattgatagc agcattaact ttagatttgc
//
