LOCUS       CP051783                2678 bp    DNA     circular BCT 08-FEB-2021
DEFINITION  Escherichia coli O157:H7 strain FLT_2018C plasmid pFLT_2018C-2,
            complete sequence.
ACCESSION   CP051783
VERSION     CP051783.1
DBLINK      BioProject: PRJNA627032
            BioSample: SAMN14648871
KEYWORDS    .
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     USMARC Feedlot Long-term E. coli O157:H7 evaluation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Bono,J.L. and Weinroth,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2020 18:51:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,714
            CDSs (total)                      :: 5,580
            Genes (coding)                    :: 5,349
            CDSs (with protein)               :: 5,349
            Genes (RNA)                       :: 134
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 100 of 231
            Pseudo Genes (incomplete)         :: 103 of 231
            Pseudo Genes (internal stop)      :: 59 of 231
            Pseudo Genes (multiple problems)  :: 24 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FLT_2018C"
                     /serotype="O157:H7"
                     /isolation_source="feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pFLT_2018C-2"
                     /geo_loc_name="USA:Nebraska"
                     /lat_lon="40.524829 N 98.140806 W"
                     /collection_date="2018"
                     /collected_by="USMARC"
     gene            complement(99..503)
                     /locus_tag="HH241_27815"
     CDS             complement(99..503)
                     /locus_tag="HH241_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH35292.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDE
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(500..772)
                     /locus_tag="HH241_27820"
     CDS             complement(500..772)
                     /locus_tag="HH241_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH35293.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     gene            complement(1131..1454)
                     /locus_tag="HH241_27825"
     CDS             complement(1131..1454)
                     /locus_tag="HH241_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QRH35294.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTVEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGNREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            1738..1932
                     /locus_tag="HH241_27830"
     CDS             1738..1932
                     /locus_tag="HH241_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QRH35295.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2120..2377
                     /locus_tag="HH241_27835"
     CDS             2120..2377
                     /locus_tag="HH241_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRH35296.1"
                     /translation="MSKRQRLRRRARGRRFSIGSAPLTSIIKTDAQVRGGETRQDYKDT
                     RRFPLEAPSCALLFLPFGLPMSFRSYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     Typing          complement(1464..1700)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          1967..2471
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.553"
                     /coverage="95.0"
                     /evalue="2.2999999999999997e-156"
     Typing          complement(1135..1454)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="1.5799999999999998e-61"
ORIGIN
        1 caagagacta cgacgacaag acaaggatct caagaagatc cttacattat gccccccccc
       61 ttggcacgcc gtaacctcgc taaacgttca gcatccagtt aatccatact accttgctcg
      121 taacgtttct ctatcaatct catttgttgc acaatatcct tcgcagaagt tctattaatc
      181 tttttattct ttctaccaaa aaacatagaa agcactccac aaataaggct tgcccaaaat
      241 acgtaccatg aaattaatat ctgccttggc atataaccat catctgatgg caaatctaaa
      301 gttaatgctg ctatcaatgc cgagccagcc acacctaaaa atatagaacc aatcgtatgg
      361 tatatatttt catcatcact aatactttct attttcccta ttaaaaaatc ccattcttca
      421 acaagaatag ggtaagccat tttctttttt ggttgagcaa tttcatagtc ctgagacatt
      481 ttaaaattat taccaccact cactttgagc ctccatcttg cttattgtca gcagataagc
      541 accctgagat aatggcatta acaagaacaa cattcccgca attatcacaa gttacaggaa
      601 taattgggac tatcggacct gccccaataa caacattacc ttcatgaaat tctcttagtt
      661 caaaaatttt gtcagaaaca ttccaacgcc cttttttaca caagggacat ttgctccctc
      721 cccacttttc agacaaatat ttaattattt gatctgactt aacatcactc atattccctc
      781 caagcattcc ttatactcat atatttatta gcgaacaatc acatcactca ataatttatt
      841 gatcaagtaa ctttatagag tacatccata tctgttttgt gcgcaaaaaa acataaatat
      901 aaaatcttac atttaccata ataccagtat tgtacgcacc gctgaaacgc gctgaacgcg
      961 attctgacgc agacaccgca aaaagagatg ttttaccccg ggaaaacagg aaggatgcgt
     1021 caggatcgtt ttcagctcgc tgcatagcta tgcatgaaag cgaatggtga tcactttggg
     1081 agcttacggt gttcataccg tctgttttcg acagttttct ctccgggaag ctaatctgcc
     1141 ataagtctgg ataacagggc acggtgatac tccgtaatag cgatcagcac ctccgcatac
     1201 tccctgttcc cgacacgctt gccgtcgata aagagttttt tctgtaacgc ccccagtctc
     1261 ataacttctt tcagtacccg atcatcagtc agtgagttaa ctttcttacc gagagctgcc
     1321 gcccgtaaaa aaccggaaac ggtcttgccg cattcagcag ccctttttct gatttcctgg
     1381 tcttcttcta ccgtcaggcg aacagggcga ctgatagccc gcctgcgcgt attacttcca
     1441 cttcgttttg tcataaaaca tggctccgta tctgacatgg gtgtcggggc aaagccctga
     1501 ccagggcaat tgtaatagcg tgcatgtatg cgcggtataa caattgcaca tcctgtcctg
     1561 tcagcaagct cgaattaccg acaaaggcac ggcagttttt tggcagtgac agtgttgtct
     1621 gacagaaatg cccgtcaaaa agccaagcgg cagaatagcg gcagcaaaac agtcgaaaag
     1681 tggtacgcga tataatcaca caacgcagat gacagacaga acggagaaaa cgaaaagatg
     1741 aacaagcagc agcaagccgt actcaacatg gcgggattca taaaaagtca gagcctgacg
     1801 ctgctcgaaa aactggacgc actcaatgcc gacgaacagg ccgccatgtg tgagaaactg
     1861 cacgaactcg cagaagaact ccagaacagc atacagacac gctttgaagc ggaaaatctt
     1921 accgggatat aacgctccag acaggatgca gaacggcata gaggagcgta aggagaaaat
     1981 gtcacataag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     2041 tgcggcgagc ggtgtcagct cactcaaagg cggtaatacg gttatccaca gaatcggggg
     2101 ataaaaccgg aaagaacatg tgagcaaaag acaaagactc agaagaaggg cgcgcgggag
     2161 gcgtttttcc ataggctccg cccccctgac gagcatcata aaaaccgacg ctcaagtcag
     2221 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2281 gtgcgctctc ctgttcctgc ctttcggttt accaatgtca ttccgcagtt atggccgcgt
     2341 ttatctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     2401 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgacttga
     2461 atccaacccg gaaagacacg acttatcgcc actggcagca gccactggta accgaattag
     2521 aggagttagc cttgaagtca tgcgcctgtt aaggctaaac tgaaagaaca agttttggtg
     2581 actgcgctcc tccaagccag ttacctcggc tcaaagagtt ggtagctctg agaaccttcg
     2641 aaaaaccacc ttgccgggtg gttttttcgt ttcataga
//
