LOCUS       CP050838                8522 bp    DNA     circular BCT 08-APR-2020
DEFINITION  Klebsiella pneumoniae strain Bckp021 plasmid pBckp021-4, complete
            sequence.
ACCESSION   CP050838
VERSION     CP050838.1
DBLINK      BioProject: PRJNA616339
            BioSample: SAMN14487838
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Ma,J., Yu,Y. and Yao,H.
  TITLE     Keeping alert to hypervirulent Klebsiella pneumoniae strains in
            genotype K1, K2, K3, K5, K54 and K57 isolated from bovine mastitis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Ma,J., Yu,Y. and Yao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2020) Department of Veterinary microbiology,
            Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu
            210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CANU v. v1.7
            Assembly Name                     :: Bckp021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171.85x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2020 12:51:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,887
            Genes (coding)                    :: 5,707
            CDSs (with protein)               :: 5,707
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 79 of 180
            Pseudo Genes (incomplete)         :: 111 of 180
            Pseudo Genes (internal stop)      :: 34 of 180
            Pseudo Genes (multiple problems)  :: 37 of 180
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Bckp021"
                     /isolation_source="milk"
                     /db_xref="taxon:573"
                     /plasmid="pBckp021-4"
                     /geo_loc_name="China: Jiangxi"
                     /lat_lon="30.7 N 118.4 E"
                     /collection_date="Jun-2018"
                     /collected_by="Nanjing Agricultural University"
     gene            1..2898
                     /locus_tag="HC680_29975"
     CDS             1..2898
                     /locus_tag="HC680_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="QIV32002.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            3046..3261
                     /gene="mbeD"
                     /locus_tag="HC680_29980"
     CDS             3046..3261
                     /gene="mbeD"
                     /locus_tag="HC680_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QIV32003.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            3725..4216
                     /locus_tag="HC680_29985"
     CDS             3725..4216
                     /locus_tag="HC680_29985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QIV32004.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIQP
                     "
                     /name="hypothetical protein"
     gene            complement(4273..4701)
                     /locus_tag="HC680_29990"
     CDS             complement(4273..4701)
                     /locus_tag="HC680_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QIV32005.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            4986..5414
                     /locus_tag="HC680_29995"
     CDS             4986..5414
                     /locus_tag="HC680_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QIV32006.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            complement(5616..5750)
                     /locus_tag="HC680_30000"
     CDS             complement(5616..5750)
                     /locus_tag="HC680_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QIV32007.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPEKHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     gene            6379..6723
                     /locus_tag="HC680_30005"
     CDS             6379..6723
                     /locus_tag="HC680_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QIV32008.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     SGKLPSLSPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6713..7723
                     /locus_tag="HC680_30010"
                     /pseudo=""
     CDS             6713..7723
                     /locus_tag="HC680_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7823..8428)
                     /locus_tag="HC680_30015"
     CDS             complement(7823..8428)
                     /locus_tag="HC680_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QIV32009.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          6112..6285
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(5642..6188)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2953..3362
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.891"
                     /coverage="89.0"
                     /evalue="2.23e-73"
     Typing          6401..6717
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="1.62e-61"
     Typing          6714..7792
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.556"
                     /coverage="89.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcacgac gccatatttt caccgaacgg cagcgagcag cgctgttcga tctgcccacg
       61 gacgaactgt cgctactgaa gttctacacg ctgggcgatg atgacctgga aaacattagg
      121 cagcgccgca gaccggaaaa caggattggc tttgccctgc aactttgtgc cttacgatat
      181 ccgggccgtg cactggctcc tggtgagatg atcccgcgtg aagtcctttc cttcgtcggt
      241 gctcagcttg gagttccggc tgatgcgctt ctcacttatg ccacacggcg ccaaacccgt
      301 cagcagcaca tggacacgct gcgcgaaatt tacggctaca agaccttcac gggccgtggt
      361 gcccgtgatc tgcgtgagtg gactttcggc caggccgaag atgccagatc aaacgaggat
      421 cttgctcatc gttttattgt gcggtgtcgg gaaacttcca ccattctgcc cgcagtatcg
      481 acaatcgagc gcttgtgcgc ggatgctctg gtcgccgctg agcggcggat tgaaacgcgg
      541 attgcggaaa atttaacagc ggatgttcgc gatcacctgg acaaacttct gagtgaaatg
      601 ctcgccggca atatcagtcg tttcatctgg cttcgcaact tcgaggttgg taacaactcg
      661 gctgctgcta accgtttgct cgacaggctc gaatttctgc gtaccctgaa tatcaatcat
      721 agtgctttgg ccagcatacc tgcccatcgc attgcccggc tgcgtcggca gggtgaacgc
      781 tacttcaccg acggtttgcg tgacatcact tcggaccgcc gctgggcgat ccttgccgtc
      841 tgtgttgtgg agtgggaagc ggcgattgct gatgccatag tcgaaaccca tgacaggatc
      901 gtaggaaaaa cctggcggga agcgaagcgc cagcatgacg aaacaatttc cggctctaaa
      961 gccacactca cggatacgat ccgtaccttc accgcgctgg gagcttcgtt gcttgaggcc
     1021 cgcagtgacg gaaccccgct ggagatggct gtcgccagtt cggttgcatg ggaccggctc
     1081 gctcaactgg tagcgacagg gactcaactc agcaacacgc tagccgatga gcctcttgca
     1141 tatgtcgggc agggatacca tcgctttcgt cgttatgcgc cccgcatgtt gcgctgtctg
     1201 aagctcgaag ccgcgccggt cgccggacca ttggtagcag cagctttgtc gatcggagag
     1261 atgaaaggtg ttgcatcgcc agaaaggcgt ttcctgcggc ccaactccaa atggaaccgt
     1321 catttacgag ctcaggaaaa aggagatacc cgtctttggg aagtggcggt actctttcac
     1381 ctccgggatg cttttcgttc cggagatgtc tggctcgctc attcgcgccg ctatggtgac
     1441 ctcaagcagg tactggtgcc gatgatcgcg gcgcaggaaa atgcaaaact ggccgtgcct
     1501 tccaacccac aggattggct ggcagacaga aaggcgcgac tcacgatcgc tcttaagcgg
     1561 ctggcccggg ctgcccgtaa cggcactatt ccgcacggta gcatagaaga tggaacgttg
     1621 cggatcgaca ggttgacagc agacgtgccg gatggtgccg aggcactcat actggatctg
     1681 tatcgccgaa tgccgtccgt tcggattacc gacatgctgc ttgaagttga tgcagccctt
     1741 ggtttcacag atgcgtttac ccatctgaga accggggctc catgtcgcga ccggatcggt
     1801 ctgctcaacg tcctgctcgc tgaagggctc aatctgggcc tgcgtaagat ggcggaagct
     1861 acaaacacgc atgattactg gcagctctca cgccttgccc gctggcatgt tgaaagcgaa
     1921 gccatgaacc aggcattggc aattgtggtg gccgcgcagg gtaaactgcc gatgtcacgc
     1981 gtctggggga tgggcacgtc agcatcgagc gatggtcagt ttttcccgac agcgcggcat
     2041 ggcgaagcca tgaacatggt caatgccaaa tatggttctg ttcccggcct caaagcgtat
     2101 actcacgtaa gcgaccagtt cgcgccattc gcttgtcagt cgatcccggc gaccgtgagc
     2161 gaggcaccgt atattctcga tggactactg atgaacgagg tcggtcgcca tgttcgcgaa
     2221 cagtatgccg atacagcagg attcaccgac catttgttcg gagccagtag cctgctcggc
     2281 tacaatctcg ttctgcgaat cagggatctg ccatcgaagc ggttgtacgt atttaatccc
     2341 gatacgaccc ccagggagtt acgcaagttg gtaggtggaa aagcccggga ggatcttatc
     2401 gttgcgaact ggcctgatat tttccgttgt gccgcgacga tgaccgctgg caaaatcagg
     2461 cccagccaac tcctgcgcaa gctcgcttct tacccacgac aaaacaacct tgcagttgcg
     2521 cttcgtgaag ttggtcgtat tgaacggacc cttttcatta ttgagtggat cctggatacg
     2581 gacatgcagc ggcgtgctca gatcggtctt aacaagggag aggcccacca tgcgctcaaa
     2641 aatgcgctcc gtatcgggag gcagggggaa attcgcgatc gcacgacaga ggggcagcac
     2701 taccgaatcg ctgggctcaa tttattgact gcggtgatca tttactggaa taccgtccat
     2761 cttggtcatg ccgtcacgga gcggcggaac gaagggttgg atgttccccc tgaatttctt
     2821 ccccacatat ccccattggg ctgggcgcac attctactga ctggcgaata tctttggccc
     2881 aaggaaccga aagcttaggg tgtcatttcg ccctcagccg gaaccgaccc ctatcaggag
     2941 agcagcgacg gccggaggtt tcttatcctg ccgccgggga tgcaaaccga agccatccct
     3001 tacgacggga cgacatggat ccgcctgaaa caggagtagc ggaagatgac agagctggaa
     3061 aagcagttgc tgagcgcatt agagcagcta cagcaggact actcgcaaag gctggacgaa
     3121 tgggagagcg ccttcgcgga atggcggagc atgtgtgggc ttatgcaacg ggagaacgcg
     3181 gtgctgaacg agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag cgagcagctg
     3241 cgccgtttgt ccgggaactg aatgccatcg aggagaaggc tgtactggag tatcagcgtc
     3301 agaacgagct ggagctgacg ctccgtcaga agcagaagag ttttgatggt ccgaagatga
     3361 ggtgacagcc cgcacgaaga agggctcatc gcgttcctgc taaataggct gactaccgga
     3421 atggtagtga cttatcccgg atgaagtgat gaaaattctt aacgttattg aagccctgct
     3481 gaactgtttt tacctggtga tggagctgtg tgagttgccg gagaggcatt tgccggtttt
     3541 taaggcgttc gcgctgattt tagtgtgact ttacgcggca tgcatctggc tgcatgacta
     3601 tgcataagtg taatatccag cggctgcgtt tccggtgttt tttcgtcatg tctgtcgcca
     3661 ctttctcgcg tctggcaccg gaaacgcagc caaccccgtt tcagctgtgc gggcaacaga
     3721 tgttatggta aatcaaccgt ccgttgataa aggttctgat aatacccatg taggtgatgt
     3781 tgtttgcttt ttatctacac actgcctgat ggagctattt atgaatgctg atgaagttac
     3841 acgaattctt gcgcaatcga gtcaaaatga ttggattgtg gatgatgaga caggggcgtt
     3901 tacttataaa aatgatttag cactgcacat taagagagct gattacgacg actacaactc
     3961 gtttaatgag ccttgggctg ttcgtcatcc tgatagtaat gcgtgttcag tcgagtacac
     4021 cgttcactat ggttcttcgt ttgttgatag aaaaaccttg gtttccgtcg atggtcatcg
     4081 tgcaaccttg cctatgcctg catccgcaac ggatctaaac gtgtcatcgg ctgatgtgaa
     4141 tttcgccaaa attgtaaaca ttggcgatcg cgttgatgaa tacattcaac gttctggtct
     4201 tacgattcag ccctaattta acgataagcc cggcctccaa atgcgggctg ttcctgcatg
     4261 taaccagcat aactaatcgg cttttttcct tactgtatat attggctttt tatgtcgcgg
     4321 ctttcttact tgctcggaca cagaaggacc aattatgtct aaataatcta caagctttgt
     4381 aaggtcgtat atgttgcggc caccatatcg gttgtcgctt tttgatgtcg gttttttatc
     4441 taagagattg gctttttcta ggtcattaat agccctttgc accgtcctta ctgacacccc
     4501 catccgtata gccaggctct ccattgaggg gtgcggccac tcctgtagat tccaccagtg
     4561 tactatgaga ttcagcaagg tattgaatgc tacagggctc aatttcttct cattttgtat
     4621 aaaaaacaac acagtaggta ctgcagtcca tcctaatgac agacctccag ccttccattt
     4681 tttttctatt gcgtaactca ttgattatcc tgtaaatatt ttttttgaga tatatataat
     4741 ataagtattc atatgaatag ggtgacacca gcgccattat tacacaacta taaaaggcta
     4801 atctgagaac agaaaaacat ttctatctgc attaaatacg gagttagtga tatgaaaacc
     4861 aaacaacatc atgatcttgt aaaagatact gaaatatttg gtgtatcctt gtctctgttg
     4921 atttccagag cgaatgagag atctgttaca atgccatctg gtctaagtag ggagcagaga
     4981 agagcatggg cagcggatca agcacgcaaa atccacaatt gaatgttgtc tctttctctg
     5041 agacattcaa tctttattac acatcccgct tttctcaaaa aacactcgat aaaatcgatg
     5101 acttcatcga acatttcgag aagtacaatc tttttggcga tgccttaaaa ggccatccgg
     5161 cttggattgg caaggtatcg ccatcttgga acgtgcctga cagttacgaa aacaaagaag
     5221 ctattgaaga acatgccagg gctaataaat tgtggcatgc ccatataggc gatcccgttt
     5281 tcatcgatac atttcacggg aagtacaaag tctctgattg ggtcatccac ttccaacgtc
     5341 tagcgccgaa ccatataaaa ctgttagagc ttgggtatca tgaccctatg caactccccc
     5401 aaaatttcga atgatctctt gttcggatga atgatccctt gagattcgtt gaccctcgtc
     5461 gtaatctctt gcggtgaaaa cgaaaaaacc acccggcaag gtggcttttt cgaaggttcg
     5521 caagagttga cgctcttttg aaccgcggta actggcttgg aggagcacag tcaccaaaac
     5581 tgtcctttca gtgtagcctt atcgcacccg aaacttcaag actctcctcc tctaaacctg
     5641 ttaccagtgg ctgctgccag tggcgctttg tcgtgttttt ccggattgga ctcaagttga
     5701 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     5761 ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     5821 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     5881 accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacca ctgatttgag
     5941 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg
     6001 gccttatcgt ttccctgtta agtttcttcc tggcatctac caggaaattt ccgccccatt
     6061 cgtaagcccg caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6121 aagcggaata tatcctgtat cgcatattct gcttacgcgc cggtgccgcc ttttttctcc
     6181 tgccacatga agcacttcac tgattcccgc acccgtgcca acatagtaag ccagtataca
     6241 ctccgctagc gctgcgtgac tggttcaggg ctgcgccccg aaacccgcta aaagccactg
     6301 acgcgcctgc ggcttgtcca accccgcgcc gaccgggaaa ggctttcccg cccgtcccgg
     6361 cgttatcagg aggccggttt gtcagagaaa cggaacaaga tgctcaccat gtgggtgacg
     6421 gaggacgagc accggcggct gcttgagcgc tgcgacggca ggcagctggc agcgtggatg
     6481 cggcagacct gcctggacga aaagccggca cgttccggaa agctcccctc actctcgccg
     6541 gcgctgctgc gccagcttgc cggtatgggc aataacctga accagatagc ccgccgcgtt
     6601 aacgccggcg gcggcactgg ccatgacagg gtgcagattg tggcggcgct gatggccatc
     6661 gatgccggac ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt
     6721 taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactacc tgctgggtaa
     6781 agaccggcag cgtgatggtg ccaccgtcct gcaggggaag ccggaagaag tccgggagct
     6841 gattgacgcg tcgccttacg tgaagaaata cacttccggc gtcctgtcgt ttgcggaggc
     6901 cgatctgcct cccggccagc gggaaaaact gatggccagc ttcgagcggg ttctgatgcc
     6961 cggacttgat aaagatcagt acagcattct gtgggtggag cacgcggaca aggggcgtct
     7021 ggagctgaac tttctgatcc ccaataccga gctgctgacc ggcaggcggc tccagccgta
     7081 ctacgaccgc gccgaccggc cgcgcatcga tgcctggcag accatcgtga acggcagact
     7141 ggggctgcat gacccgaacg cgccggaaaa ccgccgggcg ctggtcacgc cgtccggact
     7201 gccgaaaacg aagcaggagg ccgcagaagc catcacgcgt ggactgctga cccttgcttc
     7261 gtccggggag ctgaaaagcc gtcaggatgt cactgaggcg ctggaacggt caggttttga
     7321 ggtggtgcgc accacgaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat
     7381 ccgactgaag ggagccatct atgagcagtc ttttaacgct ggcgaaggac ttagagcagc
     7441 aatcgaaagc gcagaagcag agtaccggcg agatgctgaa agccgcattc agcgagcacg
     7501 agcagtctgt cagagcggaa ctgagcgcaa gcgcgaggag aatcagcgac gccatcctcg
     7561 cccacgagca gagtatgagc gaggcgatgg agaaaaaccg gcggagcgta ctgcggactg
     7621 ccgggcggac atggctcacc atcctgatgg tgtcagcgct gctgatcggc acgagcggga
     7681 gcattctgtg gtggcagggg cagcagataa ccgacaatta cacgcatctt cgtcagcagg
     7741 aagataccct ggcgaaaatg accgcccgga cgtggggagt gcgctatcag gggtcggttc
     7801 cggctgaggg cgaaatgaca ccctaagcgt tagctctgtg tcgttgcacg atgtcagcga
     7861 cggtattctt gctgataccg agctcgcgtg cgatccagcg atagctgcgt ccctcggccc
     7921 tcatcgcaac caccttaggc aaaagtcggt ctgattttgg tcgcactccg gcctgacgac
     7981 caagcctctt accacgtgcc ttcgcaacag caaggcctga cttgacccgc tcgctgatga
     8041 gatcccgctc aaactccgca atgccggaaa gaaacgtcgc cagcattcgt ccatacggcg
     8101 acgaaagatc gaacgccatt ccattcatgg ctatcacgga aaccttccag ttctccagtt
     8161 cacgtagcgt attgagcaga tcgagcgtcg agcgccccca ccgggaaagc tcagtgacca
     8221 ggattgcatc aatttgtctg gactgggcaa gcgccaggac tttctttcgc tcggcccggt
     8281 cgagtttagt tcctgaacct gtttccttaa atattcccac cacgtcgtag ccggcacggc
     8341 cggcgaaggc tcgcagatca aattcctggc gttcacaaga ctgatccgct gttgaaaccc
     8401 ggcagtaaat ggcggcacga tgtcccaatt gaaccctcct ggatttttgt atcggaacgc
     8461 cctgatttat atgggctggc tgttgtccaa aacagactat acttcaaaag ggacgaattt
     8521 gt
//
