LOCUS       CP050748               11246 bp    DNA     circular BCT 06-APR-2020
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium strain ST53
            plasmid pST53-3, complete sequence.
ACCESSION   CP050748
VERSION     CP050748.1
DBLINK      BioProject: PRJNA615288
            BioSample: SAMN14450163
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Chen,Z.
  TITLE     Genomic analyses of multidrug-resistant Salmonella Indiana,
            Typhimurium, and Enteritidis isolates using MinION and MiSeq
            sequencing technologies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Chen,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2020) Joint Institute for Food Safety and Applied
            Nutrition, University of Maryland, 5145 Campus Drive, Patapsco
            Building, Suite 2134, College Park, MD 20742, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: ST53
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/31/2020 17:46:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,813
            CDSs (with protein)               :: 4,813
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 17
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 68 of 146
            Pseudo Genes (incomplete)         :: 79 of 146
            Pseudo Genes (internal stop)      :: 26 of 146
            Pseudo Genes (multiple problems)  :: 25 of 146
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /strain="ST53"
                     /serovar="Typhimurium"
                     /isolation_source="Diarrheal patient"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pST53-3"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.23 N 121.47 E"
                     /collection_date="2011-07-14"
                     /collected_by="Shanghai Municipal Center for Disease
                     Control and Prevention"
     gene            complement(join(11157..11246,1..531))
                     /locus_tag="HEC88_25355"
     CDS             complement(join(11157..11246,1..531))
                     /locus_tag="HEC88_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="QIT67220.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKAFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVRMFE"
                     /name="ParA family protein"
     gene            826..1470
                     /locus_tag="HEC88_25360"
     CDS             826..1470
                     /locus_tag="HEC88_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QIT67211.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSLQSSGE"
                     /name="recombinase family protein"
     gene            complement(1586..1765)
                     /locus_tag="HEC88_25365"
     CDS             complement(1586..1765)
                     /locus_tag="HEC88_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QIT67212.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(3063..3272)
                     /locus_tag="HEC88_25370"
                     /pseudo=""
     CDS             complement(3063..3272)
                     /locus_tag="HEC88_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            3337..4041
                     /locus_tag="HEC88_25375"
     CDS             3337..4041
                     /locus_tag="HEC88_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="QIT67213.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(4092..4907)
                     /locus_tag="HEC88_25380"
                     /pseudo=""
     CDS             complement(4092..4907)
                     /locus_tag="HEC88_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4917..5105)
                     /locus_tag="HEC88_25385"
     CDS             complement(4917..5105)
                     /locus_tag="HEC88_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QIT67214.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            6207..7046
                     /locus_tag="HEC88_25390"
     CDS             6207..7046
                     /locus_tag="HEC88_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QIT67215.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVKRVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
                     /name="replication initiation protein"
     gene            7067..7909
                     /locus_tag="HEC88_25395"
     CDS             7067..7909
                     /locus_tag="HEC88_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QIT67216.1"
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLEVLEINETLHIR
                     RKTESGALATKACPSLFSSTMRHVNYDKIIRYNSGLNLLVNELSFSARRVFFICLYYYF
                     ENKLESDKSLLIRADKYAEFVGVERSVAYKQMKDAADFFSRNKNLITHCDYISNEGLLR
                     VMFSSKTINYITAIDSRKYQVTELPLKSALSLQGRYSWPLYQLIKFSHLKSFTIGVDEL
                     KKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
                     /name="replication initiation protein"
     gene            7913..8359
                     /locus_tag="HEC88_25400"
     CDS             7913..8359
                     /locus_tag="HEC88_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QIT67217.1"
                     /translation="MAKIYQFPQGEERAKFRKEIARERKKRFAVKTGSTFVKWLGWGWF
                     YLRLLAASVLHFIFVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSSEISQQ"
                     /name="hypothetical protein"
     gene            8496..8849
                     /locus_tag="HEC88_25405"
     CDS             8496..8849
                     /locus_tag="HEC88_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="QIT67221.1"
                     /translation="MKYLVKEFINEKYSKAVNILKDNLKEHYHVFYGVRLSEILFPTSE
                     YGTDAFFKEFELINSVILPLVIFDFIDRKPIMVIGFEEAPGIDSLIDSGMEVVLLDGLS
                     DLLLVEKLMPLFD"
                     /name="DNA distortion polypeptide 3"
     gene            complement(9387..10451)
                     /locus_tag="HEC88_25410"
     CDS             complement(9387..10451)
                     /locus_tag="HEC88_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QIT67218.1"
                     /translation="MKTISLFNNKGGVGKTTLTWNLSVSLSAKGKSVLLIDFDPQCNLS
                     IALLGETGFSDLLKKDANNPYGKTIRAFCQPFIQQDILPVVHTFPPQFPMEPGSGRLDI
                     APGDFWLNNLSDVISVGTDLIAGNAVNRFLIPSLIASKAQELNNCEYDYVLIDVPPSFN
                     TIVRSALYCSDYFLVPCTADIFSAYCIGLIGETLPKFRDDWEQGIRRTSAQNNSLISQK
                     GSPKFAGWVFNGFDKRKNPGQQNSSEIAADAAHRDKIIDSVKNDLRPKLASIKTYSAVA
                     SFTPDTPVAKVEDLNVMAPDSILQGVPLRYLSTVAPTRGVLARGAWSGPQKALMQTMSA
                     EHDVLADYIIQNFV"
                     /name="AAA family ATPase"
     gene            complement(10875..11105)
                     /locus_tag="HEC88_25415"
     CDS             complement(10875..11105)
                     /locus_tag="HEC88_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA partition complex ParG"
                     /protein_id="QIT67219.1"
                     /translation="MSLEKAHTSVKKMTFGENRDLERVVTAPVSSGKIKRVNVNFDEEK
                     HTRFKAACARKGTSITDVVNQLVDNWLKENE"
                     /name="DNA partition complex ParG"
     Typing          7914..8359
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaattgat ttcacgtaaa cctgacgttg tgtaatagct gtacgaaatg ctttaacacc
       61 ggtgtctttg atactttctt taagcacatt caacatggtt gccatttcta tcttacgggt
      121 gatcagaaag cgggcttcaa ctttgcggct gtaagcctgt gcttccagaa cagtaacgac
      181 gcttcctgct gcggagaaat ccagagggct gggggtgaca gggataatta ccagatcgct
      241 taccatgacg gctgcggagg tgattactga gagtgaacct gccccgtcca caatagcaaa
      301 gtcatagtcc gccagatctt ttcggattcc atagacgtct ttttcagatg ctgctgtaaa
      361 tacgtcaaat gctgccttgc ccgctttgct ccagttcgtc aggctcattt gtggatctgt
      421 atctaccaca gcaatgttgt atccgctcct gctcaacgca gtggctatgt tgattacggc
      481 agttgtttta cctgaacccc ctttcggatt cagaaatgag atcaccttca tactcaaatc
      541 ctcaattgtg tgtctactca attactcctg tgagtttttg agtttatcat actctttgta
      601 tgagcaaagg ttaatatgag tttatgtgta tatgagttaa ggagtatcta ctcatttgag
      661 ttgtcttgtg tatatgagtg tttgagttta tgagtgtgta gtgacaaggt cacatctact
      721 cattatttgt tttttggttc ttttagagtg tgcctttcct gtcgatccac tttctggttc
      781 attttgggta tagtggtgtg accttatgcg aactggttct gtaatatgac ttcaaatgca
      841 ccaacaaact ttgtccgcgc ttatctgaga gcttcaacag cagaacagga tgcttcgcgt
      901 gccctggaaa ctatcgaggc ttttgccagg gaaagaggcc tgatcatttg cagctattat
      961 attgagaatg aatccggctc ccgtcttgac cgcccggaac tctttcgtct tctgagagat
     1021 tgccagcaaa atgacatttt gcttatcgag gatgtggaca gactttcccg actgtcaggg
     1081 gaagactgga atacgctgaa gaaaatgatc cgtcagaaag atatccgggt tatggccgtg
     1141 aatgtgccga cgacctggat taattctggt atgagtgagt ttgacagccg gttgtttgca
     1201 gccattaacg atatgctgct ggatatgctg gccgctgttg ccagaaggga ctatgaacaa
     1261 cggcgggaac gccagaaaca ggggatagag aaagcaagga aagacggcaa atataaaggg
     1321 cgtaaaccta atcaagccag acatgatgca attatcaggt tgatagaaag tggcagctcc
     1381 tggacacagg tacagaaggt gctagggtgc agccgcggca caatcagtag cgcaataaaa
     1441 cgtaaatccc tgcaatcctc cggtgagtag ggtagggtga gctcaataaa ctggttgacg
     1501 ttatttatga gggaggtgct gaagtggata tttttaaaaa gcaaattgaa tgttgtaact
     1561 aatgagtatc aaaataaaag gccgcttacg cgacctcagc cttttgcggt tttggcttgg
     1621 tgatatacca cgatagacaa ccgacaataa aactcatgaa cattccagta aaacgatcat
     1681 cgatgcctgg aaacaggcaa ccgacaaaca tcgcaacagg gaaaggaaga agtaaacagc
     1741 gccccaaaat gctcataaaa tccataacac ctcctttgtt aaagggcagg aagcggtgca
     1801 cttcctgccc ttagttacgt ttccgcagtc atcaatcgat gaactgacgg atcagttcca
     1861 gaactgcaat cagcgcgttt gcaagcgcag ctaccgccct cagaacgatg attaacaggt
     1921 taatcatccg ttttctccat aaatggagta aagacaccgt ggcttgctct tacgctgcct
     1981 gtcaccacgg cttctcttcg tcttgtcagt tgtagagagt ctcactccta ccagagcaca
     2041 gataaccagc accacctgta ttacctgtca ggactttgaa gctgttcatg ccgcatttag
     2101 cggaatgcag ggaaagactc accacagcac taaccctctg attatacgtt tatctaactt
     2161 tcaagcaaac cgttaacgcg tagtcaccgc ctgtcatatt gcataatcag gcttcacgga
     2221 ttatcatact gttgtcagtt gtagagagtt tctcttccgg gcgagttacc agctcgtacc
     2281 ggaactgtaa aaaagccgac ctcaccagtc ggttttttta cgtctgcagt ttcccgcttc
     2341 atttagatct attttaaatg aataaatttt ctgcggcaaa cccaacgacg ttgggtcgtt
     2401 agtgtaaata attgttcaaa actcataaac actcctttgt taaagggcag gaagcggtaa
     2461 acgtctctcc cttagttaag ccatcaatca ctcgattagt ttctgaatgg caatcagcgc
     2521 gtttgcaatg gcgagcacag ccctgagaac gatgattaac agattcatca tccgtttctc
     2581 taggttgata ctggaggaag tcaccgtagc ttgctcatac atcgcctgtc acaacggctc
     2641 tctcacattg ttggatgtag agtaccttcc ctctaccaga gcactgaaca cagcaccacc
     2701 cgtttgttca gccaggactt taattcgttc atccccagat agcgcagggg ttagaagcaa
     2761 gggagacaca ctgtacagcg ccaacacaaa gattatacag atgcatgcat aaaattcatt
     2821 taaagaaatt accattgaca gacaatctga agtcaaacta gacaacaggt gtctagtgcc
     2881 ataatactat gagtgttggg tgtagagtat cttcctccag tcagagccac caactcaagg
     2941 ctggaaaaca caaaaaagcc aaattaccag tcggtttttt ttgtgcttgt cgttcagctg
     3001 tacttccgtt ttttcccccc ttcattgaag aagtagtagg cagagcaggt aatgactcca
     3061 acttattgat agtgttttat gttcagataa tgcccgatga ctttgtcatg cagctccacc
     3121 gattttgaga acgacagcga cttccgtccc agccgtgcca ggtgctgcct cagattcagg
     3181 ttatgccgct caattcgctg cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg
     3241 cgggattcat acagcggcca gccatccgtc atcggcactg ttgcaaatag tcggtggtga
     3301 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     3361 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     3421 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     3481 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     3541 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     3601 gcgtatctgt accgggccgt cgacagccgg ggccgcactg tcgattttta tctctcctcc
     3661 cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa cgtgaagaag
     3721 tggcagatcc cacgattcat caacacggat aaagcgcccg cctatggtcg cgcgcttgct
     3781 ctgctcaaac gcgaaggccg gtgcccgtct gacgttgaac accgacagat taagtaccgg
     3841 aacaacgtga ttgaatgcga tcatggcaaa ctgaaacgga taatcaacgc cacgctggga
     3901 tttaaatcca tgaagacggc ttacgccacc atcaaaggta ttgaggtgat gcgtgcacta
     3961 cgcaaaggcc aggcctcagc attttattat ggtgatcccc tgggcgaaat gcgcctggta
     4021 agcagagttt ttgaaatgta aggcctttga ataagacaaa aggctgcctc atcgctaact
     4081 ttgcaacagt gccctccgtt gtaatgagcc agtcgggaag ccgctattgg tggcttcttc
     4141 agataacgac ccaggtagcg tgccgtattc cgccctccgg ctgtcttctt cgacatgtac
     4201 acatgccagt attttccgcc ggatttcagc accaggcttc tccactgtga ttccgtcgtg
     4261 atatgtgaca acgactccgg cattgccatc ccctctgacc acgctttcag aagcagctgc
     4321 cgcatattcc acatccaccg tgaacgcatc gcgtctttca ggaagctcag ctttttccac
     4381 tgaccatgct tattcagacc tccacaggtt acagacacat gtacatgcgg atgccagttg
     4441 agacgacggc catacgtgtg gatggcgcag aagataccgg gttccagccc ccgttttcgg
     4501 gcggcataca gcagattctc caccgccaga cggcacacgt cattcagcag ccagcggttg
     4561 ctttcgaaca ccggccacag cgtgtccggc agggtgaaga ccagatgtac ccagtcgcag
     4621 tcaggaagac gattcagctg tgttgctgtc cacaggtctg tggccttctt tccgcaggac
     4681 gggcaggcac ggctgccgca tgagttggtc aggtacttta cgtgctgaca gtccgggtta
     4741 tcacacccga actcttttac acccagtatc cgtgtgccgc aggccagcat tttggtgacg
     4801 gcttcaacct cgatatcgcg cagaccgccc gcatccagga aggacgtcca gcactggttg
     4861 gctgtgaaca gacgtttcag agggcgggga gtaaaaccgg acaacatgag gatggattaa
     4921 accccggtat cctgtagcag ggtaagggct gctgtatcgc cacggcgctt caggatggag
     4981 gtcgtcacca tcgcaggtgg tgggtccatg ggtttgagca ttttcatcca gtcccattct
     5041 cgttgtgaga gcttctcagc accaacagag agcagaactg tatcgatact tttgcgggtc
     5101 aacataaggg gatgagtcct taataaaaca ggtcctcaga gcatacatat tctgcggggc
     5161 gtgcggcaac aacaggacga accggggcgg ttttcaattt gcagccgcca ggctgccgtg
     5221 gttcaatctt gatcaaaatt tcctcaaggc aacgactgtt ttgtaaatac cctctaaaac
     5281 gcgctgtatc gcattctgag cgattttttc gatttcacat ggttttcctc atggttacat
     5341 cgaacaaagc gttagaaagc gattcagggc ttctcagtga aagatccaaa aaggatcaat
     5401 caacggatcg tatgatctga actcttctgg ttttgagaaa ggagacaccg ctcgccgcag
     5461 ccgaacgacc gagtgtagcg agttagtgag cgaggaagcg gaacagcaca gggataacat
     5521 caggcaatta cgcgcagtgg ttaaaaagcg agcatattgt ttttatgtgt ggttgtcttg
     5581 gttcagagtg atctgtcctc tgggtttacg caaagttatc cacaggtagt gtgaattttt
     5641 tgttttgagc aaagttatcc acagattttt tgtgtttttt tgcttattaa acttcttgtt
     5701 tttaagatct taaatgtaga tcggtagatc tactttaaga tctttaactt actctaactt
     5761 attctgcaaa cattgtgcca gtttttttat caataatttc attaagatag gtgttgtttt
     5821 ttgtcgcctc aaaatgaaag gtggatgggt acgcactgaa aggtggatgg gtacgcactg
     5881 taattttcat gaaaggtgga tggctgcgca ctgaaaggtg gatgggtacg cactgaaagg
     5941 tggatgggta cgcatttttc tgtttttatt cagatattcg tatctttgta tcttcatttc
     6001 tttagttctt tatagtgctc taatctaacg tatgacgtta aaaggtggat acctgcgcat
     6061 ttctatcata ttgaatataa atgataaaaa ttctttcatg tgatccgtgt tttggtggaa
     6121 acatataagg tggataaacc actttacata tttccgcctt tgtggttatg atttcaccat
     6181 aagaacttaa aaaccaaagg taatgtatga acttactgag cgttacaaag aaaacaaaaa
     6241 ttcgccacag aaatgaaatt aacagcacat tctcatcgtt gcctttgtct gccagaagaa
     6301 ttttgtttat ggctatggcg cagattgatt cacgtgaaga gttatctgag ggggcaacct
     6361 ttcgtattac ggctcgggaa tatgcattta tcgctgatat tgatgtaaca ggtgcttacc
     6421 ggcagttgaa agagggggca gaagaacttc aggccagtgt tattcgtatt cctcgaaatc
     6481 aacttctcac agctcagggc aatcaaagct ctggacatac gaagaagaga ggtaaattac
     6541 cttctgatgc agtcaggctt atgaacctga ctgcgttttg tgattatgtt gaaagtgaag
     6601 gatatattga tattgctttt tcacatgtca ttgagccata tatcacgatg ttaaaggatt
     6661 cttacactac tcaggttttg atatcgtctg taagactcgc tgatcaaaat tcaaatatgc
     6721 tttatcagtt tatcaggaaa caatacagca gtggtaaaaa gacatttttc gacattgaag
     6781 tagatgtatt aaaggatgaa cttgaattat tcagaataga taatggcgaa aaagtttacc
     6841 tttatcagaa ttttaaggat tttaataagg tttttctgca aaaaaatatt gaaaaaataa
     6901 atcaatatac agagattaat catctcgaag ttaaaattgt taagagagtc gcaagaagag
     6961 catcaaagct aagattttcg tataaaatag ataaagaaag cgaaggactt gataccagaa
     7021 taccgtatgg tttcagaggt gcttaatata taaaggtaaa tacactatgg agcaaataaa
     7081 attacataaa caaggtggtg cggcagtaat tatcattcct gctagtatga tagaaaatac
     7141 aggatgggaa gatggaacct tattggaggt actagaaata aatgagactc ttcatataag
     7201 aagaaagaca gaatcaggag ctttagctac taaagcttgt ccttctcttt tttcatctac
     7261 tatgaggcat gttaattacg ataaaattat tagatataat agcggattaa atctgttagt
     7321 taatgaactg tcattttcgg caagaagggt cttttttatt tgtttatatt attactttga
     7381 aaataaactt gaatcggata agtcgctatt gatccgggct gataagtatg cagaatttgt
     7441 tggtgttgag cgttctgttg catataagca aatgaaggat gcagcagatt tcttttcaag
     7501 aaataaaaat ttaattacac attgtgacta tatttcgaat gagggattgc ttcgtgttat
     7561 gttttcgtct aagacgataa attatattac agcgatagac tcccgtaaat atcaggttac
     7621 cgagcttcct ctaaaatctg ctttgtctct tcaaggaaga tactcatggc ctttatatca
     7681 actgataaaa tttagccatc ttaaatcttt cactataggt gtagatgaac ttaagaaaga
     7741 aataggttat attcaaaatc aaaagttttc tttctttaaa agagatggaa tcaatatagc
     7801 tattgatgaa ataacagaaa ggacagatgt taaaaacatt aaatgtgtta atgctgaaag
     7861 gcaggggaga agagtcagca aagtccgttt tgagatcgaa atgaggtaac tcatggctaa
     7921 aatctatcaa ttccctcagg gggaagaacg agctaaattc aggaaagaaa tcgccaggga
     7981 gcgtaagaaa cgttttgcag tgaaaacagg gagcactttt gttaagtggc ttggctgggg
     8041 atggttttat cttcgtcttc ttgccgcgag tgttttacat tttatctttg tttttgtgtt
     8101 agccattctt ggtgcgttta aatgggctgt gttctggctg gggggtattt tgtgtgtcat
     8161 cacctggtat catctcgatc accagttatg gacgccacaa aatctgacta taccggtgat
     8221 tacggcaacc tggattttgg gactgctggc tgagcctctg atggagctgc tcaacaaaac
     8281 tatgccctgg cacaagttac ttgttcccga acctgcggac gaaagcaccg agaacagtag
     8341 cgagataagc cagcaatgat gctactcagc agttatgcca gtccctgcct tttcactgcg
     8401 atgcaggata gggcaaacgc cgcaaaatga cgtctctgac gccattccgc aacgtttgcc
     8461 cggtgaccta cagtcggttc ttattggaga tgtttatgaa atatctggtc aaagagttta
     8521 ttaacgagaa atattctaag gctgttaata ttttaaagga taatcttaaa gaacactatc
     8581 atgtttttta tggtgtgaga ttaagtgaga ttctttttcc taccagtgaa tatggcacag
     8641 atgctttctt taaagaattt gaattaatta acagtgtaat acttccatta gtaatatttg
     8701 attttataga ccgaaagccg atcatggtta tcggctttga ggaagcgcct gggattgatt
     8761 cacttattga ctctggtatg gaagttgttt tgttagatgg tttaagcgat ctgctactgg
     8821 ttgaaaaact gatgccattg tttgattaaa tccctggaaa tacatgttac ccattcgtat
     8881 taatgcattt tacacgaacg atgtttattc catttacctg ttttcataac ttctccccta
     8941 cggagaacta accaaactga tctgcatcta tgatcttaat tatataaaat ttggggggcg
     9001 tgattctaat gtcctcattg tattggtaac aatctttttt tcatcgtttt tattttgata
     9061 tttaatattg ctctcaattg atttttaccg tttcttcatt caatgatatg agcgtcaaat
     9121 tgattagata atggtgattt ttaccctcta tcatcaaagc ttgtgaagct attaatatac
     9181 aaaaaatttt cagtcgtttt tctattgtct cacatatttt taagagagaa tctgtatgct
     9241 gttcgccatt cacttatgta ggccacttcg tactaatcgc ttcaatctaa agaacttcac
     9301 ttttccttag cgtttgttat cgcagaaatt cccacggaac ccctaagacg gtaaaagggg
     9361 tgccggcgta caatccctaa aagatattaa acgaaatttt ggattatata atcagctaaa
     9421 acgtcatgtt ctgccgacat agtctgcatt agggctttct gaggtcctga ccaagcccct
     9481 ctggccaaca cccccctagt aggtgctacg gtagataaat atctgagcgg gaccccctgt
     9541 aagatactat ctggagccat aacatttagg tcttcaactt ttgcgacagg agtgtctgga
     9601 gtaaaagaag ccactgctga gtatgttttt atagatgcta atttggggcg aaggtcattt
     9661 tttactgaat caattatttt atctctatgg gctgcatcag ccgcgatctc tgatgagttt
     9721 tgttggcctg gattctttct tttatcaaat ccgttgaaga cccaacctgc aaatttaggt
     9781 gaaccttttt ggcttattag agagttattt tgggctgaag ttcttctaat accctgttcc
     9841 cagtcgtctc taaattttgg taaagtttca ccaatgagtc ctatgcagta agccgaaaat
     9901 atatcggcag tacatggaac caagaaataa tcagaacagt agagagccga tctgactatc
     9961 gtattaaatg acggcggtac atcaataaga acataatcgt attcacaatt atttaactct
    10021 tgagctttac tagcaattaa actaggtatc aagaatctgt ttactgcgtt tccagcaatt
    10081 aaatctgtac ctacactaat cacatctgaa agattattga gccaaaagtc acctggagct
    10141 atgtctaacc ttcctgagcc aggctccatg ggaaattgtg gtgggaatgt atgtacgaca
    10201 gggagaatat cttgttgtat aaatggttgg caaaatgctc ttatcgtttt accataagga
    10261 ttatttgcat ccttctttaa aaggtcagag aagccagtct caccaagtaa agctattgat
    10321 aaattgcatt gaggatcgaa atctatcagc aaaacagatt ttccctttgc cgatagagat
    10381 acacttaagt tccaagtcag ggtcgttttt cctaccccgc ctttgttgtt aaatagtgaa
    10441 atagttttca ttttattcct ttcttagaca ttttcaatgt ttagaaataa ctacatcgga
    10501 gaatagtgta atacgtaatc gattgcttga tacccgtata taaccatcaa catgcacgac
    10561 aaaccttaaa attgaagttt ccgtacagtt tctcattccg acagagtttg tcacgcaaaa
    10621 atttacaggt ttatagattt aattttcttt aactaactgc aggggctgaa gatttctttc
    10681 agtgagaaat cgcttagcgg gtgttatcac agaaattccc acggacccca ttttgtccga
    10741 actcctactg atagattaaa tcctgaatca tctaagtttt gcagaatcaa gtaactattg
    10801 gctgttgtac atagccaggg ctgctttgag aacagttgtt ttgcgtcggc ctgtctgttc
    10861 gcttcggctt ccaattattc gttctctttg agccagttat ctacaagctg gttcaccacg
    10921 tcagtgattg atgtaccttt cctggcgcat gcagccttaa accgggtgtg cttttcttca
    10981 tcaaaattaa cgttaacacg tttgattttt ccagatgata ctggtgctgt tactactcgt
    11041 tccagatctc tgttttcacc aaaggtcatt ttttttactg acgtatgcgc tttttcaagt
    11101 gacattcagg ctactccggt ttttatactt atttgattat atgagtaaat gtgtaattac
    11161 tcaaacattc taactatctc ttttgtaagg atttctattt caccttttgc agcaccatca
    11221 ctggattcaa acacgctgtc gccatc
//
