LOCUS       CP049996                9166 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt214 plasmid pER05716.3A.3, complete
            sequence.
ACCESSION   CP049996
VERSION     CP049996.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331031
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190.163959398137x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/11/2020 12:46:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,883
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 63 of 103
            Pseudo Genes (incomplete)         :: 31 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 18 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt214"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER05716.3A.3"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            join(7523..9166,1)
                     /locus_tag="FPO77_14290"
                     /pseudo=""
     CDS             join(7523..9166,1)
                     /locus_tag="FPO77_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /name="phage tail tape measure protein"
     gene            2..1168
                     /locus_tag="FPO77_14295"
                     /pseudo=""
     CDS             2..1168
                     /locus_tag="FPO77_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /name="terminase large subunit"
     gene            1184..2349
                     /locus_tag="FPO77_14300"
                     /pseudo=""
     CDS             1184..2349
                     /locus_tag="FPO77_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /name="phage portal protein"
     gene            2333..3070
                     /locus_tag="FPO77_14305"
     CDS             2333..3070
                     /locus_tag="FPO77_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="QOA12248.1"
                     /translation="MKVEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDII
                     INSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMIHN
                     PSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELIEMMAKETWLNADEAIEQ
                     GFADSKMFENDNMQIVASDTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEKINMK
                     EKESEIDVADSKLSANGFSRFLF"
                     /name="Clp protease ClpP"
     gene            3093..4238
                     /locus_tag="FPO77_14310"
                     /pseudo=""
     CDS             3093..4238
                     /locus_tag="FPO77_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /name="phage major capsid protein"
     gene            4295..4543
                     /locus_tag="FPO77_14315"
     CDS             4295..4543
                     /locus_tag="FPO77_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12255.1"
                     /translation="MEHNQHKYKVGELYPAEGYNNPRVELLTNQIKNKYDKVYIVPLDK
                     LTKQELLELCESLQKKASSSMVKSEIIDLLNGEDNDD"
                     /name="hypothetical protein"
     gene            4533..4817
                     /locus_tag="FPO77_14320"
     CDS             4533..4817
                     /locus_tag="FPO77_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="QOA12254.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFEL
                     ENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
                     /name="phage head-tail adapter protein"
     gene            4801..5163
                     /locus_tag="FPO77_14325"
     CDS             4801..5163
                     /locus_tag="FPO77_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="QOA12249.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCWA
                     SIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQVSP
                     DLDNKDFIMIRGGYSS"
                     /name="head-tail adaptor protein"
     gene            5160..5564
                     /locus_tag="FPO77_14330"
     CDS             5160..5564
                     /locus_tag="FPO77_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12250.1"
                     /translation="MSVKVTGDKALERELEKHFGIKEMVKVQDKALIAGAKVIVEEIKK
                     QLKPSEDSGALISEIGRTEPEWIKGKRTVTIRWRGPFERFRIVHLIENGHVEKKSGKFV
                     KPKAMGGINRAIRQGQNKYFETLKRELKKL"
                     /name="hypothetical protein"
     gene            5561..5968
                     /locus_tag="FPO77_14335"
     CDS             5561..5968
                     /locus_tag="FPO77_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12251.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIVI
                     DDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELKMG
                     NVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
                     /name="hypothetical protein"
     gene            5969..6613
                     /locus_tag="FPO77_14340"
     CDS             5969..6613
                     /locus_tag="FPO77_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="QOA12252.1"
                     /translation="MAIKHASAPKAYFNITGLGFAKLTKEGAELKYSDITKTRGLQKIG
                     VETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEKQG
                     KQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALFPLV
                     DNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
                     /name="phage tail protein"
     gene            6655..6879
                     /locus_tag="FPO77_14345"
     CDS             6655..6879
                     /locus_tag="FPO77_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12256.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSDG
                     QVTAEAQGIATVKATVGNMSDTITINVEA"
                     /name="hypothetical protein"
     gene            6928..7278
                     /locus_tag="FPO77_14350"
     CDS             6928..7278
                     /locus_tag="FPO77_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12253.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLIDD
                     IEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQGQQ
                     TEETRNFIQNMK"
                     /name="hypothetical protein"
     gene            7305..7466
                     /locus_tag="FPO77_14355"
     CDS             7305..7466
                     /locus_tag="FPO77_14355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOA12247.1"
                     /translation="MLKNMDTLMMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEK
                     AEALIDAF"
                     /name="hypothetical protein"
ORIGIN
        1 aataataaac cgtgcaacta aatcggttat tcgatataac acatcaaaca caaaaaccaa
       61 agacggtgga cgtgagggtg tgttattttt gatgaaattc attatttctt tggtcctgaa
      121 atggtaaacg tcaaacgtgg tggattaggt aaaaaagaaa aatagaagaa cgttttatat
      181 aagtactgat ggttttgtta gagagggtta tatcgatgca atgaagcaca aaattgcaag
      241 tgtattaagt ggcaaggtta aaaatagtag attgtttgct ttttattgta aattagacga
      301 tccaaaagaa gttgatgaca gacagacgtg ggaaaaggcg aacccaatgt tacataaacc
      361 gttatcagaa tacgctaaaa cactgctaag cacgattgaa gaagaatata acgatttacc
      421 attcaaccgt tcaaataagc ccgaattcat gactaagcga atgaatttgc ctgaagttga
      481 ccttgaaaaa gtaatagcac catggaaaga aatactagcg actaatagag agataccaaa
      541 tttagataat caaatgtgta ttggtggttt agactttgca aacattcgag attttgcaag
      601 tgtagggcta ttattccgaa aaaacgatga ttacatttgg ttaggacatt cgtttgtaag
      661 acaagggttt ttggatgatg tcaaattaga acctcctatt aaagaatggg aaaaaatggg
      721 attattgacc attgtcgatg atgatgtcat tgaaattgaa tatatagttg attggttttt
      781 aaaagctaga gaaaaatatg ggcttgaaaa agtcatagct gataattata gaactgatat
      841 tgtaagacgt gcgtttgagg atgctggtat aaaacttgaa gtacttagaa atccaaaagc
      901 aatacatgga ttacttgcac cacgtatcga tacaatgttt gcgaaacata acgtaatata
      961 tggagacaat cctttgatgc gttggtttac taataatgtt gctgtaaaaa tcaagccgga
     1021 tggaaataaa gagtatatca aaaaagatga agtcagacgt aaaacggatg gatttatggc
     1081 atttgttcat gcgttatata gagcggatga tatagtagac aaagacatgt ctaaagcact
     1141 tgatgcatta atgagtatag atttctaata gaggaggtga gacatgagta ttctagaaaa
     1201 gatatttaaa actaggaaag atatatcata tatgcttgat ttagatatga tagaagattt
     1261 atcacaacaa gcgtatgtga aacgtttagc gattgatagt tgtattgaat ttgttgcgcg
     1321 agctgtcgct caaagtcatt ttaaagtatt ggaaggtaat agaattcaaa agaatgatgt
     1381 ttactacaag ttaaatataa aaccaaatac tgacttatca agcgatagtt tttggcaaca
     1441 agttatatat aaactaattt acgataacga ggttttaatc gtagtaagtg acagcaaaga
     1501 attacttatc gcagatagct tttacagaga agagtatgct ttgtatgatg atatattcaa
     1561 agatgtaacg gttaaagatt atacttatca acgtactttc acaatgcaag aggtcatata
     1621 tttaaagtac aacaacaata aagtgacaca ctttgtagaa agtctattcg aagattacgg
     1681 gaaaatattc ggaagaatga taggtgcaca attaaaaaac tatcaaataa gagggatttt
     1741 gaaatctgcc tctagcgcat atgacgaaaa gaatatagaa aaaattacaa gcgttcacaa
     1801 ataaattatt caatactttt aataaaaacc aactagcaat cgcgcctttg atagaaggtt
     1861 ttgattatga ggaattatct aatggtggta agaatagtaa catgcctttt tctgaattga
     1921 gtgagctaat gagagatgca ataaaaaatg ttgcgttgat gattggtata cccccaggtt
     1981 tgatttacgg agaaaacagc tgatttggaa aaaaacacgc ttgtatttga gaagttctgt
     2041 ttaacacctt tattaaaaaa gattcagaac gaattaaacg cgaaactcat aactcaaagc
     2101 atgtatttga aagatacaag aatagaaatt gtcggtgtga ataaaaaaga cccacttcaa
     2161 tatgctgaag caattgacaa acttgtaagt tctggttcat ttacaaggaa tgaggtgcgg
     2221 attatgttag gtgaagaacc gtctgacaat cctgaattag acgaatatct agtgacgaag
     2281 aactacgaaa aagcaaacga aaatggtagt acattgaaag gtggtgatga agatgaaagt
     2341 ggagattaaa ggcgtcatcg tttccaacga agataaatgg gtttacgaaa tgcttggtat
     2401 ggattcgact tgtcctaaag atgttttaac acaactagaa tttagtgatg aagatgttga
     2461 tattataatt aactcaaatg gtggtaacct agtagctggt agtgaaatat atacacattt
     2521 aagagctcat aaaggcaaag tgaatgttcg tatcacagca atagcagcaa gtgcggcatc
     2581 gcttatcgca atggctggtg accacatcga aatgagtccg gttgctagaa tgatgattca
     2641 caatccttca agtattgcgc aaggagaagc gaaagatcta aatcatgctg cagaaacatt
     2701 agaacatgtt ggtcaaataa tggctgaggc atatgcggtt agagctggta aaaacaaaca
     2761 agaacttata gaaatgatgg ctaaggaaac atggctaaat gctgatgaag ccattgaaca
     2821 aggttttgcg gatagtaaaa tgtttgaaaa cgacaatatg caaattgtag caagcgatac
     2881 acaagtgtta tcgaaagatg tattaaatcg tgtaacagct ttggtaagta aaacgccaga
     2941 ggttaacatt gatattgacg caatagcaaa taaagtaatt gaaaaaataa atatgaaaga
     3001 aaaggaatca gaaatcgatg ttgcagatag taaattatca gcaaatggat tttcaagatt
     3061 ccttttttaa tacaaaaata ggaggtcata aaatgactat aaatttatcg gaaacattcg
     3121 caaatgcgaa aaaacgaatt tattaatgca gtaaacaacg gtgaaccgca agaaagacaa
     3181 aatgaattgt acggtgacat gattaaccaa ctatttgaag aaactaaatt acaagcaaaa
     3241 gcagaagctg aaagagtttc tagtttacct aaatcagcac aaactttgag tgcaaaccaa
     3301 agaaatttct ttatggatat caataagagt gttggatata aagaagaaaa acttttacca
     3361 gaagaaacaa ttgatagaat cttcgaagat ttaacaacga atcatccatt attagctgac
     3421 ttaggtatta aaaatgctgg tttgcgtttg aagttcttaa aatccgaaac ttctggcgtg
     3481 gctgtttggg gtaaaatcta tggtgaaatt aaaggtcaat tagatgctgc gttcagtgaa
     3541 gaaacagcaa ttcaaaataa attgacagcg tttgttgttt taccaaaaga tttaaatgat
     3601 tttggtcctg cgtggattga aagatttgtt cgtgttcaaa tcgaagaagc atttgcagtg
     3661 gcgcttgaaa ctgcgttctt aaaaggtact ggtaaagacc aaccgattgg cttaaaccgt
     3721 caagtacaaa aggtgtatcg gtaactgatg gtgcttatcc agagaaagaa gaacaaggta
     3781 cgcttacatt tgctaatccg cgcgctacgg ttaatgaatt gacgcaagtg tttaaatacc
     3841 actcaactaa cgagaaaggt aaatcagtag tggttaaagg taatgtaaca atggttgtta
     3901 atccgtccga tgcttttgag gttcaagcac agtatacaca tttaaatgca aatggcgtat
     3961 atgttactgc tttaccattt aatttgaatg ttattgagtc tacagttcaa gaagcaggta
     4021 aggttttaac gtacgttaaa ggtctatatg atggttattt agctggtggt attaatgttc
     4081 agaaatttaa agaaacactt gcgttagatg atatggattt atacactgca aaacaatttg
     4141 cttacggcaa agcgaaagat aataaagttg ctgctgtttg gaaattagat ttaaaaggac
     4201 ataaaccagc tttagaagat accgaagaaa cactataaaa ttttatgagg tgataaaatg
     4261 gtgaaatttt aaagttgtta gaggatttaa agacatagag cacaatcaac acaagtacaa
     4321 agtaggggag ttgtatccag ctgaagggta taacaatcct cgtgttgaat tgttgacaaa
     4381 tcaaatcaaa aataagtacg acaaagttta tatcgtacct ttagataagc tgacaaaaca
     4441 agaattatta gaactatgcg aatcattaca aaaaaaagcg tctagttcaa tggttaaaag
     4501 tgaaatcatc gacttattga atggtgaaga caatgacgat tgatgatttg cttgtcaaat
     4561 ttaaatcact tgaaaagatt gaccataatt cagaggatga gtacttaaag cagttgttaa
     4621 aaatgtcgta cgagcgtata aaaaatcagt gcggagtttt tgaattagag aatttaatag
     4681 gtcaagaatt gatacttata cgcgctagat atgcttatca agatttatta gaacacttca
     4741 acgacaatta cagacctgaa ataatagatt tttcgttatc tctaatggag gtatcagaag
     4801 atgaagaaag tgtttaagaa acctagaatt acaactaaac gtttaaatac gcgtgttcat
     4861 ttttataagt atactgaaaa taatggtcca gaagctggag aaaaagaaga aaaattatta
     4921 tatagctgtt gggcgagtat tgatggtgtc tggttacgtg aattagaaca agctatctca
     4981 aacggaacgc aaaatgacat taaattgtat attcgtgatc cgcaaggtga ttatttaccc
     5041 agtgaagaac attatcttga aattgaatca agatatttca aaaatcgttt gaatataaag
     5101 caagtatcac cagatttgga taataaagac tttattatga ttcgtggagg atatagttca
     5161 tgagtgtgaa agtgacaggt gataaagcat tagaaagaga attagaaaaa cattttggca
     5221 taaaagagat ggtaaaagtt caagataagg cgttaatagc tggtgctaag gtaattgttg
     5281 aagaaataaa aaaacaactc aaaccttcag aagactcagg agcgctgatt agtgagattg
     5341 gtcgtactga acctgaatgg ataaagggga aacgtactgt tacaattagg tggcgcgggc
     5401 cttttgaacg atttagaata gtacatttaa ttgaaaatgg tcatgttgag aaaaagtcag
     5461 gaaaatttgt aaaacctaaa gctatgggtg ggattaatag agcaataaga caaggtcaaa
     5521 ataagtattt tgaaacgtta aaaagggagt tgaaaaaatt gtgattgata ttttgtacaa
     5581 agttcatgaa gtgattagtc aagacagaat tattagagag cacgtaaata tcaataatat
     5641 taagttcaat aaatacccta atgtaaaaga tactgatgta ccttttattg ttattgacga
     5701 tatcgacgac ccaataccta caacttatac tgacggagat gagtgtgcat atagttatat
     5761 tgtccaaata gatgtttttg ttaagtacaa tgatgaatat aatgcgagaa tcataagaaa
     5821 taagatatct aatcgcattc aaaagttatt atggtctgaa ctaaaaatgg gaaatgtttc
     5881 aaatggaaaa ccggaatata tagaagaatt taaaacatat agaagctctc gcgtttacga
     5941 gggcattttt tataaggagg aaaattaaat ggcaataaaa catgcaagtg cgccaaaggc
     6001 gtattttaac attactggtt taggtttcgc taaattaacg aaagaaggcg cggaattaaa
     6061 atatagtgat attacaaaaa caagaggatt acaaaaaatt ggtgttgaaa ctggtggaga
     6121 actaaaaaca gcttatgctg atggcggtcc aattgaatca gggaatacag acggagaagg
     6181 taaaatctca ttacaaatgc atgcgttccc taaagagatt cgcaaaattg tttttaatga
     6241 agattatgat gaagatggcg tttacgaaga gaaacaaggt aaacaaaaca attacgtagc
     6301 tgtatggttc agacaagagc gtagagacgg tacatttaga acagttttat tacctaaagt
     6361 tatgtttaca aatcctaaaa tcgatggaga aacggctgag aaagattggg atttctcaag
     6421 tgaagaggtt gaaggtgagg cacttttccc tttagttgat aataaaaagt cagtacgtaa
     6481 gtatatcttt gattcagcta acatgacaaa tcatgatgga gacggtgaaa aaggcgaaga
     6541 ggctttctta aagaaaattt taggcgaaga atatactgga aacgtgacag agggtaacga
     6601 agaaactttg taacaaaacc ggcttcatcg gaaactgcgg taaagtcggt taatatacca
     6661 gatagcatta aaacacttaa agttggcgac acatacgatt taaatgttgt agtagagcca
     6721 tctaatcaaa gtaagttatt gaaatacaca acagatcaaa cgaatattgt atcaatcaat
     6781 agtgatggtc aagttactgc ggaagcacaa ggtattgcta cggttaaagc aacagttggt
     6841 aatatgagtg acactataac aataaatgta gaagcataag aggggcaacc cctctatttt
     6901 atttgaaaat aaggagagta ttataaaatg gcaaaattaa aacgtaacat tattcaatta
     6961 gtagaagacc cgaaagcaaa tgaaattaaa ttacaaacgt acttaacacc acacttcatt
     7021 tcatttgaaa ttgtatacga agcaatggat ttaatcgatg atattgagga cgaaaatagc
     7081 acgatgaaac caagagaaat cgctgacaga ttgatggata tggttgtaaa aatttacgat
     7141 aaccaattca cagttaaaga cctaaaagaa cgtatgcatg cacctgatgg aatgaatgca
     7201 cttcgtgaac aagtgatttt cattactcaa ggtcagcaaa ctgaggaaac tagaaatttt
     7261 atccagaaca tgaaataaag cctgaagatt taacatataa agcaatgttg aaaaatatgg
     7321 atactctcat gatggactta attgaaaatg gtaaagacgc taacgaagtt ttaaaaatgc
     7381 catttcatta tgtactttcc atatatcaaa ataaaaacaa tgacatttct gaagaaaaag
     7441 cagaggcttt aattgatgcg ttttaacctt aaccgtttgg ttagggttat ttttttgaac
     7501 ttttttagaa aggaggtaaa aaatgggaga aagaataaaa ggtttatcta taggtttgga
     7561 tttagatgca gcaaatttaa atagatcatt tgcagaaatc aaacgaaact ttaaaacttt
     7621 aaattctgac ttaaagttaa ccggtaacaa cttcaaatat accgaaaaat caactcatag
     7681 ttacaaacaa aggattaaag aacttgatgg aactatcaca ggttataaga aaaacgttga
     7741 tgatttagcc aagcaatatg gcaaggtatc tcaagaacag ggcgaaaaca gcgcggaagc
     7801 tcaaaaatta cgacaagaat ataacaaaca agcaaatgag ctgaattttt tagaaaaaga
     7861 actagaaaaa acaacaactg agtttgaaga gttcaaaaaa gctcaagttg aagctcaaag
     7921 aatggcagaa agtggctggg gaaaaaccag taaagttttt gaaagtatgg gacctaaatt
     7981 aacaaaaatg ggtgatggtt taaaaatcca ttggtaaagg tttgatgatt ggtgtaactg
     8041 cacctgtttt aggtattgca gcagcatcag gaaaagcttt tgcagaagtt gataaaggtt
     8101 tagatacagt tacccaagca acaggagcaa ccggcggaga gcttaagaag ttgcagaatt
     8161 catttaaaga tgtttatggc aactttccag aagacgctga gactgtaggc ggtgttttag
     8221 gggaagttaa cacaaggtta ggtttcactg gcaaagaact tgagagtgcc acagagtcat
     8281 tcttgaaatt tagtcacata acaggttctg acggcgtaca agccgttcaa ttaattacgc
     8341 gtgcaatggg tgatgcaggt attgaagctg atgagtatca aagtgtactt gatatggtag
     8401 cgaaagcagc acaggctagc ggtataagtg ttgatacatt agctgatagc attactaaat
     8461 acggtgctcc aatgagggct atgggctttg agatgaaaga atcaatcgct ttattctctc
     8521 aatgggagaa atcaggtgtt aatactgaaa tagccttcag tggtttgaaa aaagctatat
     8581 ccaattgggg taaagcgggt aaagacccaa gagaagaatt taagaagaca ttagcagaaa
     8641 ttgaaaggac accggatata gctagcgcaa caagtttagc gattgaagca tttggtgcaa
     8701 aagcaggtcc tgatttagca gatgctatta aaggcggtcg ctttagttac caagattctt
     8761 aaaaactatc gaagattcgc aaggaacggt caatcagaca tttaaagatt ctgaaagtgg
     8821 ctccgaaaga tttaaagtag caatgaataa acttaaatta gtaggtgctg atgtatgggc
     8881 ttctattgaa agtgcgtttg ctccagtcat ggaagaatta atcaaaaagc tatctgtagc
     8941 agttgattgg ttttcaagtt taagtgatgg atctaaaagt cgattgttat attcgtggta
     9001 ttgctgctgc aattggtcct gtagtttttg gattaggtgc attcataagc acagttggca
     9061 acgcagtaac tgtattagct ccattattag ctagtattgc aaaggctgac ggattgatta
     9121 gttttttatc aactaaagtg cctattttag gaacagtctt cacagc
//
