LOCUS       CP049577               13763 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt174 plasmid pER04882.3A.2, complete
            sequence.
ACCESSION   CP049577
VERSION     CP049577.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12330991
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13763)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13763)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194.377495910461x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 17:04:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,974
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,790
            CDSs (with protein)               :: 2,790
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 73 of 102
            Pseudo Genes (incomplete)         :: 32 of 102
            Pseudo Genes (internal stop)      :: 17 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt174"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER04882.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            25..1095
                     /locus_tag="FPO37_14800"
     CDS             25..1095
                     /locus_tag="FPO37_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="QNZ95540.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIEREM
                     IESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGGFV
                     SVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGA
                     TIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHL
                     TGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPL
                     GLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNMKSYEQS
                     YTKEALFDKMIKDALN"
                     /name="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            1112..1726
                     /locus_tag="FPO37_14805"
     CDS             1112..1726
                     /locus_tag="FPO37_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="QNZ95541.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSES
                     FITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALKNI
                     FDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAAMIL
                     LISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
                     /name="phosphatase PAP2 family protein"
     gene            complement(1835..2339)
                     /gene="tnpA"
                     /locus_tag="FPO37_14810"
                     /pseudo=""
     CDS             complement(1835..2339)
                     /gene="tnpA"
                     /locus_tag="FPO37_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /name="tnpA"
     gene            2632..2781
                     /locus_tag="FPO37_14815"
     CDS             2632..2781
                     /locus_tag="FPO37_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ95546.1"
                     /translation="MDIIFNYYLYISENHCQLETKTFFEYFLRIVNKTHDYAILENKKI
                     CISH"
                     /name="hypothetical protein"
     gene            2857..2988
                     /locus_tag="FPO37_14820"
     CDS             2857..2988
                     /locus_tag="FPO37_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ95542.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
                     /name="hypothetical protein"
     ARG             3247..3389
                     /name="arlR"
                     /gene="arlR"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="100.0"
                     /coverage="21.46"
     ARG             3405..3703
                     /name="arlR"
                     /gene="arlR"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="100.0"
                     /coverage="45.21"
     gene            3897..5249
                     /gene="arlS"
                     /locus_tag="FPO37_14830"
                     /pseudo=""
     ARG             3898..5039
                     /name="arlS"
                     /gene="arlS"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="99.21"
                     /coverage="84.26"
     gene            5533..8315
                     /locus_tag="FPO37_14835"
                     /pseudo=""
     CDS             5533..8315
                     /locus_tag="FPO37_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /name="2-oxoglutarate dehydrogenase E1 component"
     gene            8329..9590
                     /gene="sucB"
                     /locus_tag="FPO37_14840"
                     /pseudo=""
     CDS             8329..9590
                     /gene="sucB"
                     /locus_tag="FPO37_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /name="sucB"
     gene            10170..10979
                     /locus_tag="FPO37_14845"
     CDS             10170..10979
                     /locus_tag="FPO37_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="QNZ95543.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADIS
                     PGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNKYF
                     SLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQILT
                     NIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQVEFET
                     KDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
                     /name="VOC family protein"
     gene            11008..11211
                     /locus_tag="FPO37_14850"
     CDS             11008..11211
                     /locus_tag="FPO37_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ95544.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETEE
                     YYQIIDNSGLVGGYYKTYFKEV"
                     /name="hypothetical protein"
     gene            11392..12181
                     /locus_tag="FPO37_14855"
                     /pseudo=""
     CDS             11392..12181
                     /locus_tag="FPO37_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /name="MoxR family ATPase"
     gene            12195..12680
                     /locus_tag="FPO37_14860"
     CDS             12195..12680
                     /locus_tag="FPO37_14860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ95545.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYYN
                     PVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKFYQ
                     QLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKHNIQIYYFCI"
                     /name="hypothetical protein"
     gene            12671..13763
                     /locus_tag="FPO37_14865"
                     /pseudo=""
     CDS             12671..13763
                     /locus_tag="FPO37_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815526.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
ORIGIN
        1 tttaaaagaa tagaggtaat attaatgacg aaaatcgcat ttaccggagg gggaacagtt
       61 ggacacgtat cagtaaattt aagtttaatt ccaactgcat tatcacaagg ttatgaagcg
      121 ctttatattg gttctaaaaa tggtattgaa agagaaatga ttgaatcaca actaccagaa
      181 attaagtatt atcctatttc gagtggtaaa ttaagaagat atatttcttt agaaaatgcc
      241 aaagacgtat ttaaagtatt gaaaggtatt cttgatgctc gtaaagtttt gaaaaaagaa
      301 aaacctgatc tattattttc aaaaggtgga tttgtatctg tgcctgttgt tattgcagcc
      361 aaatcattaa atataccaac tcttattcat gaatctgact taacaccagg attagcgaat
      421 aagatagcac ttaaatttgc caagaaaata tatacaacat ttgaagaaac gctaaactac
      481 ttacctaaag agaaagctga ttttattgga gcaacaattc gagaagattt aaaaaatggt
      541 aatgcacata atggttatca attaacaggc tttaatgaaa ataaaaaagt tttactcgtt
      601 atgggtggaa gcttaggaag taaaaaatta aatagcatta ttcgcgaaaa cttagatgca
      661 ttattacaac aatatcaagt gatacattta actggtaaag gattaaaaga tgctcaagtt
      721 aaaaaatcag gatatataca atatgaattt gttaaagagg atttaacaga tttattagca
      781 attacggata cagtaataag tagagctgga tcaaatgcga tttatgagtt cttaacatta
      841 cgtataccaa tgttattagt accattaggt ttagatcaat cccgaggcga ccaaattgac
      901 aatgcaaatc attttgctga taaaggatat gctaaagcga ttgatgaaga acaattaaca
      961 gcacaaattt tattacaaga actaaatgaa atggaacagg aaagaactcg aattatcaat
     1021 aatatgaaat cgtatgaaca aagttatacg aaagaagctt tatttgataa gatgattaaa
     1081 gacgcattga attaatgggg ggtaatgctt tatgagtcaa tggaaacgta tctctttgct
     1141 catcgttttt acattggttt ttggaattat cgcgtttttc cacgaatcaa gacttgggaa
     1201 atggattgat aatgaagttt atgagtttgt atattcatca gagagcttta ttacgacatc
     1261 tatcatgctt ggggctacta aagtaggtga agtctgggca atgttatgta tttcattact
     1321 tcttgtggca tatctcatgt taaagcgcca caaaattgaa gcattatttt ttgcattaac
     1381 aatggcatta tctggaattt tgaatccagc attaaaaaat atattcgata gagaaagacc
     1441 tacattgctg cgtttaattg atataacagg atttagtttt cctagcggtc atgctatggg
     1501 atcaactgca tattttggaa gtggtatcta tctattaaat cgattaaatc aaggtaattc
     1561 aaaaggtatt cttatagggt tatgtgcagc tatgatttta ttgatttcca tatcacgtgt
     1621 atatctaggt gtacattatc caacagatat tattgccggc attattggtg gattattttg
     1681 cattatttta tcaacgttat tacttagaaa taaattaata aattaaatag taaaatcaaa
     1741 accacccgtg tgaacgggtg gtttgttctg cggctataag ccttccttac tggccagccc
     1801 taaaagggca ctgacaagtc agccaactgc actactattc cagcaaccct aaagggttac
     1861 tcttttttct ttctttttta tttttctctc cagtgaaagg atctaaatat tcttccattg
     1921 agatttggtc tgcaacgata tcctcttgta attgattacg aatataattt tcaatcactt
     1981 ttttatttct acctactgta tccacataaa atcctttaca ccaaaacttt ctatttccat
     2041 atctatactt taagttagca tgtctatcaa atatcattaa actacttttt ccttttaaat
     2101 agccaacaaa tgatgatacc ccaagtttgg gtggtatact aactaacata tggatatgat
     2161 ctttacatgc ctctgcttca attatctcta caccttttct ttcacataat tgacgcaata
     2221 taatccctat atcttttttt atttttccat atatcacttg tcttctgtat ttaggtgcaa
     2281 agacaatgat acttacaatt ccattttgta tgtgctaaac tgtttgtgtc agatgacatt
     2341 aaatagcatc tcctcgtgtt gattattttg gttggctgac caatatttat tctagcacgt
     2401 agagatgcat tttttgtgac aatggtagaa ccttttctga accatacgca tagcgtatgg
     2461 ttttcttttt acaaaaaaca aaagcagtaa acctaaagtg tcgtaagggt ttactgcttt
     2521 tataaaacgt tgttataacg tatattgtct tttacgggca tataaaaggg gatattttga
     2581 aaatgaccat ccaacaagaa cgaaacgttg tggggggatg ttctatatgg tattgatatc
     2641 attttcaact actatttata cattagtgag aatcattgtc aattagaaac taaaactttt
     2701 tttgaatatt ttttaagaat agtaaataaa acgcatgatt acgctatttt agaaaataaa
     2761 aaaatttgta tttctcatta gaattagaat atttaaagtg atgaggttta aacattatat
     2821 tgtttacata ctccttttga attcatacat tatgaaatgt tacttccaag ttcaaaatcg
     2881 cacattgaaa tgatgtgtga aatgtttaaa ctacggtcat tttgtgaaaa taaagataat
     2941 aactattcat tttacaatag tgaaaagtca gtatatgaca acaattaata ttgcggtaag
     3001 gtgtgttaca gtattctata tttaagtact gcaatcagaa ttaacagaat gccattaact
     3061 gattattaaa tatttgagtt aataaataat taatgattgt agcttgaaaa atttaaaaca
     3121 tggttattga tttgtgataa aatttaaacg taaacaaact aatttaaaaa gcaactattg
     3181 tatagaaaaa caaaatttaa aaatatatta ccttattaga aaaagtcgta atatgaggtg
     3241 tacaaatgac gcaaatttta atagtagaag atgaacaaaa cttagcaaga tttcttgaat
     3301 tggaactcac acatgaaaat tacaatgtgg acacagagta tgatggacaa gacggtttag
     3361 ataaagcgct tagcattact atgatttaat catattagat ttaatgttgc cgtcaattaa
     3421 tggcttagaa atttgtcgca aaattagaca acaacaatct acacctatca ttataattac
     3481 agcgaaaagt gatacgtatg acaaagttgc tgggcttgat tacggtgcag acgattatat
     3541 agttaagccg tttgatattg aagaactttt agcaagaatt cgtgcaattt tacgtcgtca
     3601 gccacaaaag gatattatcg atgtcaacgg tattacaatt gataagaacg cttttaaagt
     3661 gacggtaaat ggcgcagaaa ttgaattaac aaaaagagta tgatttacta tatcttctag
     3721 ctgaaaataa aaaccatgtt atgcaacggg aacaaatttt aaatcatgta tggggttata
     3781 atagtgaagt agaaacaaat gtcgtagatg tttataagat atttacgaaa caagttaaaa
     3841 ccatacgatc gtgacaaaat gattgaaaca gttcgtggcg ttgggtatgt gatacgatga
     3901 caaaacgtaa attgcgcaat aactggatta ttgttaccac gatgattacg tttgtcacga
     3961 tatttttgtt ttgtttaatt attatttttt tcttgaaaga tacactgcat aatagtgagc
     4021 ttgatgatgc agaacgaagc tcaagcgata ttaataattt atttcattct aagcctgtta
     4081 aagatatatc tgcattagac ttgaatgcat ctttaggtaa ttttcaagag ataattattt
     4141 atgatgagca taataataaa ttatttgaga catcgaatga taacacagtg agagttgaac
     4201 caggttatga acaccgttat tttgaccgcg taataaaaaa acgctataaa ggcattgaat
     4261 atttaattat taaagaacca attacaacgc aagatttcaa agggtatagc ttgttaattc
     4321 attcactaga aaattatgat aacatcgtaa aatcattgta tatcattgcg ctggcatttg
     4381 gagtgattgc aacaattata actgccacaa tcagttatgt attttcaaca caaattacta
     4441 aaccgcttgt cagtttatca aataaaatga ttgagattcg acgagatggt tttcaaaata
     4501 aattgcaatt aaatacaaat tatgaagaaa tagataattt agcaaatacg tttaatgaga
     4561 tgatgagcca aattgaagaa tcatttaatc aacaaagaca atttgttgaa gatgcgtcac
     4621 atgaattacg aacaccatta caaattattc aaggtcattt aaatttgatt cagcgatggg
     4681 gaaaaaaaga cccagcagta ttagaagaat cgttaaatat ttctattgaa gaaatgaatc
     4741 gtatcataaa attagtcgaa gaattacttg aattgactaa aggagatgta aatgacattt
     4801 cttctgaagc acagaccgtg catattaatg atgaaattcg ctcgcgaata cactcattaa
     4861 aacaattgca tcctgattat caatttgata cggatctgac atctaaaaat ctagaaatta
     4921 aaatgaaacc tcatcaattc gaacaattat ttttaatctt tattgataat gcaatcaaat
     4981 atgatgtgaa gaataagaaa attaaagtta agacaaggtt aaaaataagc aaaaaataat
     5041 tgaaattaca gatcatggaa ttggtattcc agaggaagat caagatttca tttttgatcg
     5101 cttttatcga gtggataaat ctcgttcaag aagtcaaggc ggtaatggac tcggattatc
     5161 tattgctcaa aaaatcattc aattaaacga ggatcgatta aaattaaagt gaaattaaca
     5221 aaggaacaac gtttaaaatc atattttaat catgactgag acgtcaatca aagtcatagg
     5281 atcaattttt taagtacaca ttagctgtga ctaatgtata agaacaacta taaaacaaat
     5341 aaacagtggt tctttatcat ttctgttgta ctcccaaaat ttacaataaa atacatctat
     5401 aaacctagaa gaatcaacgc ttttgttgat tcttcttttt agcagataaa taggtaaatc
     5461 tactttaaca aataactaaa tagtgatatt attacattgt aagcgtttca acatttttgt
     5521 ggagggtgta aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt
     5581 gcgaatttag gtctaatgtt agatctatat gatgactttt tacaagatcc atcatctgta
     5641 ccagaagatt tacaagtctt attcagcaca attaagaatg atgactcaat tgtaccagct
     5701 ttaaaaagta caagtagtca aaatagcgac ggcacaatta agcgtgtcat gcgtttaatt
     5761 gataatattc gccaatacgg gcatcttaaa gccgatattt atcctgtaaa tcctccaaaa
     5821 aggaaacatg tacctaaatt agagattgaa gactttgatt tagatcaaca gactttggaa
     5881 ggtatatcag caggaattgt ttcagatcac tttgccgaca tttatgataa tgcttatgaa
     5941 gcaattttaa gaatggaaaa acgttacaaa ggaccaattg catttgagta tacacatatt
     6001 aataacaata ccgaacgtgg ttggttaaaa agaagaattg aaacgccata taaagtaacg
     6061 ttaaataata acgaaaaaag ggcactattc aaacaattag cgtatgttga agggtttgaa
     6121 aaatatcttc ataaaaactt cgttggtgca aagcgttttt caattgaagg ggtagacgca
     6181 cttgtaccga tgttacaacg tactattacg attgctgcga aagaaggtat taaaaatata
     6241 caaataggca tggctcaccg tggacgttta aacgttttaa cgcatgtctt agaaaaaccg
     6301 tacgaaatga tgatttcaga atttatgcat acagatccaa tgaaattctt acctgaagat
     6361 ggtagcttgc agttaactgc tggatggact ggtgatgtga aatatcacct tggtggcatt
     6421 aaaactactg attcatacgg tacaatgcag cgtattgcac tggctaacaa tccaagtcac
     6481 ttggaaattg ttgcacctgt tgttgagggg cgtacgagag cagcacaaga tgatacacaa
     6541 cgagctgggg ctccgacgac tgatcatcat aaagcaatgc caattattat acatggcgat
     6601 gctgcttatc ctggtcaagg aattaacttc gaaacaatga acttaggaaa cttgaaaggc
     6661 tattctacgg gtggttcatt gcatattatt actaacaata gaattggatt tactacagaa
     6721 ccaattgatg cacgttcaac aacttattct acagatgtgg ccaaaggtta tgatgtgcca
     6781 atattccatg tcaatgcaga tgacgttgaa gctactattg aagcaattga tattgcaatg
     6841 gaatttagaa aagagtttca taaagacgtc gttattgatt tagtaggtta tcgtcgtttc
     6901 ggacataacg aaatggatga accatcaatt actaatccag ttccttatca gaatattcgc
     6961 aaacatgact ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca
     7021 gaagatgaaa tgcattcatt tatagaacaa gtccaaaagg aactaagaca agctcatgat
     7081 aaaattaata aagctgataa aatggataat ccagatatgg aaaagcctgc agatcttgca
     7141 ttaccgttac aagcagacga acaatcatta cttttgatca cttgaaagaa ataaatgatg
     7201 cattgttaac atatccggat ggctttaaca ttttgaaaaa gttaaacaaa gttcttgaga
     7261 agcgtcatga gccgtttaat aaagaagatg gtttagttga ttgggcacaa gcagaacaac
     7321 ttgcatttgc gacaatttta caagatggta cacgattcgc ttaactggtc aagagtgaac
     7381 ggtgtacatt cagtcatagg catgccgtgt tacatgatga gcaaacaggt gaaacatata
     7441 caccttttac atcatgttcc tgatcaaaag cgacatttga tatacacaat tctccgcttt
     7501 cagaagcagc agtagttttt tttggctata taatgtggaa aacaaaaaaa gcttcaatat
     7561 ttgggaagca aatatggtga ttttgcaaat atgtcacaat gatttttgac ttattcagtt
     7621 ctcgctcaaa atggggagaa cgttcaggat taacattatt cttacctcat gcatatgagg
     7681 gtcaagggcc tgaacattca tcagcaagat tagagcgatt tttacaatta gctgctgaaa
     7741 ataattgcac agttgtcaac ttatctagtt caagtaatta tttccactta ttgcgtgcac
     7801 aagcggctag tttagattct gaacaaatgc gaccattggt tgttatgtca ccaaaaagct
     7861 tactgagaaa taaaacagtt gcaaaaccaa ttgatgaatt tacttctggt ggatttgagc
     7921 caattttgac agaatcatat caagcggata aggttacaaa agttattttg gcaactggta
     7981 aaatgttcat tgatttaaaa gaagcattag ctaaaaatcc agacgaatca gtattactcg
     8041 ttgcgattga aagattgtat ccattcccag aggaagagat tgaagcatta ctagcacaat
     8101 tgccaaacct tgaagaagtg tcatgggtac aagaagaacc taaaaatcaa ggtgcatggt
     8161 tatatgtcta tccatatgtt aaagtgctag ttgcagataa atatgattta agttatcatg
     8221 gcagaattca aagggctgct ccagctgaag gcgatggaga aattcataaa cttgttcaaa
     8281 ataaaattat agaaaatgca ttaaaaaata actaggggga aataagtcat gccagaggtt
     8341 aaagttccag aattagcaga atctattaca gaaggtacca ttgcagaatg gttgaaaaac
     8401 gtaggggata gcgtagaaaa aggtgaagct attcttgaat tagaaactga taaagttaat
     8461 gtcgaagttg tatctgaaga agcaggtgta ttatctgaac aacttgcaag tgaaggcgac
     8521 actgtagaag ttggacaagc aattgctatc atcggcgaag gtagtggcaa tgcttctaaa
     8581 gaaaatagta acgacaatac tccacaacaa aatgaagaaa caaataataa aaagaagaaa
     8641 caacaaataa ttcggtagat aaagctgaag taaatcaagc aaatgacaat cagcaacgta
     8701 ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa tggtgtgaat cttgctgaag
     8761 taagtccgaa aacaaatgat gtggttcgta aagaagatat tgataagaaa caacaggcac
     8821 cggcatcaac acaaacaaca caacaagcat ctgcaaaaga agagaaaaaa tacaatcaat
     8881 atcctacaaa accagtgatt cgtgaaaaaa tgtcacgtag aaagaaaaca gctgccaaaa
     8941 aattattaga ggtatctaat aatacagcta tgttaacaac atttaacgaa gttgacatga
     9001 caaatgttat ggaattgcgt aaacgtaaga aagaacaatt tatgaaagat catgatggta
     9061 ctaaattagg atttatgtca ttctttacta aagcttctgt agcagctttg aaaaagtatc
     9121 cagaagttaa tgcagaaatc gacggcgacg acatgattac gaaacaatat tatgatattg
     9181 gtgtagctgt ttctacagat gatggattat tagtaccatt tgtaagagat tgtgataaaa
     9241 gaattttgca gaaatcgaag cagaaattgc taatttagca gttaagcacg agagaaaaaa
     9301 cttggcttag atgatatggt taatggttca tttacgatta caaatggcgg tatttttgga
     9361 tcaatgatga gtacgccaat tatcaatggt aatcaagctg caatcttagg catgcattca
     9421 attattacaa gaccaattgc gattgatcaa gatacaatcg aaaatcgtcc aatgatgtat
     9481 attgcattaa gctatgatca tagaattatt gacggtaaag agcagttgga ttcttaaaaa
     9541 caattaaaga attaattgaa aacccagaag acttattatt agaatcttaa tccacaacac
     9601 aaaatagttt actattccta aaagcgggat taaaaatcaa taacaaacag cagtaagatt
     9661 atttcctagt cgaaatatct tactgctgta ctttattttt ataatgattc gtattaaaat
     9721 gtggtttaat tttgactact taaaaatata tcatttctat tgaaatagac tcacaataga
     9781 aatataggta gaatagcgtg tttcaatatg ctaatgaatg cattttagat aataacaaac
     9841 gatgaaatat atgaattcta taagcgcctt tagttaattt aaaatcatct gaacatgatg
     9901 taaattcgct ttatggacac gacatatata tgtttttcat gtcctacaaa aaacgaaata
     9961 ttcaattgct atatacagat attcattaat gacacactca atagcaacca taacaatatt
    10021 atgttaccta tttaaatgaa atagttcttt taaagaaata catttttcac atattaatct
    10081 ataatcaaaa tcaactgacc gatattctat aatttatgat taaaataagt tataatataa
    10141 tagtaaagat aaagatagag gtggctataa tgtgtggact tagaagtata acattaggta
    10201 caacaaatat agaacagaca aaacatttca tggttgacat attaggatta aattatgaag
    10261 aacttcttga aaactcaatt cgtttcggcg atgcagatat aagcccagga acaagacttc
    10321 aatttataca agttccaagt gagcaattag aagaatctca ctttgtgggt attggattac
    10381 gtacaccaac tgactcaggt ttagaggagt atgcggaaat attatcgaat aaggatattc
    10441 catttacaac agttaaagaa ttaaatggca ataaatattt cagtctcgaa gataacaatg
    10501 gtcatatttt ctcaatatat tcaaacgaga ataattatgg cgttggttta ggtatgcctt
    10561 cttttgagag tgcggtcaat ccgttacatc aagtgcaagg tttaggacca gtgattctta
    10621 aagtgaatca tgtagatatt acaggtcaaa ttttaacaaa tatattcgga cttgaagtat
    10681 ttgcagaata ccaacccttc gacaatgctg actatcatgt ccaagtattc aaagttggaa
    10741 cgggtggtct aggtggcgaa atacatttaa tgcctgtaga aaccgaaatg acaatgccgg
    10801 aatatggcgc agtcgatcaa gttgagtttg aaacgaaaga tgcagatttc tttaatcaag
    10861 cgaaatcgcg cttagatgaa gtggaaatac catatcaaac gcttgagcaa gatgatattg
    10921 aatcaattag aattactgaa aacagtggat tatcgtttat attcacttta caaaaataat
    10981 ttttttacga tagcgaggac aaaatttatg ttacatgaaa cttggaaaga acgtacacca
    11041 atcaagaaag tagaagtcat taatacagat gcaaagaaat ttacagtttc tgacatgtta
    11101 acggttggca aacaatatga tgttatcaac gaaactgaag aatattatca aataatagat
    11161 aattctggat tagttggcgg ttactataaa acatatttca aagaagttta aacgcataat
    11221 tattgaaatg aagcctaata tgagattttc aattagtaaa tttggtaaaa tataaacaat
    11281 tcgcatacta agcattgcgt tttatatcag aatagaatag taaactaagg catttggaga
    11341 acttattcca tgtcttagtt ttttaattta tttaaaggag aacgttttaa aatggcacta
    11401 aaacattata agaattcaga ttcaacagtt ttcaatgatg cgaaggcatt atttgattta
    11461 aataaaaata ttttacttaa aggtccaaca ggttcaggga aaacaaagtt ggcagaaaca
    11521 ttaagtgaag ttgttgatac acccatgcat caagtcaatt gttctgttga tttagataca
    11581 gaaagcttat taggctttaa aacaattaaa acaaatgcgg aaggtcaaca agaaattgtc
    11641 tttgtagatg gtccagttat taaagctatg aaagaggggc atattttata tattgatgaa
    11701 ataaatatgg ctaaacctga aacattgcct gtattaaatg gggtcttaga ttatcgtcgt
    11761 caaattacga atccatacac tggtgaagta atcaaagctg taccaggatt taacgttata
    11821 gcagcgataa atgaaggtta tgttggtact ttgccaatga atgaagcact aaaaaatcgc
    11881 tttgttgtta ttcacgttga ttatattgat ggggacattt taaaaaatgt gattaaagag
    11941 caaagtttat tacaaggtga taaacaaatc gaacaaatta ttaagtttaa tgaagattta
    12001 cgtactatgt ctaagcaggg acaaatttct gaagaagcct agtatccgtg cattattaga
    12061 cttgtgtgat ttaatcactg taatgccagt tgaacgtgca attaaacgta caattattga
    12121 taaattggaa gatgaacgtg aacaacaagc aatatataat gctgtagaac taaactttta
    12181 aagagggata aaacatgagt gatcgtttca taaaatttaa tgacgaacag ttagatgcca
    12241 agcaagtaat gatgttgcaa gacttagctc gactcttatt aaaaaatgaa caaacacaag
    12301 ttaaaatcca aaaattccct tactataatc ctgttcaaaa tgttttgatt acgagttggt
    12361 tttggtcaca tcgaccaagc catatcgaaa tggctggttt aaaaacagat gttatgttag
    12421 ctgcttacgg ttatcatatg atggatgtac aaattgtaaa tgaagtagta caagataaaa
    12481 cttttaaaca ccctaaattt tatcaacaac tttttaaatt attagaagat atgcgcgtat
    12541 taaattctat taaagttgag cgcccaagta ctgctaaatt aattgatctt cgcttagata
    12601 cacgtatatc atatacggaa tctcaaatta aagtgtatcg tactaaacac aatatacaga
    12661 tttattattt ttgtatttag aacatgcatt tttaagccaa gatttctttg atataccatc
    12721 tatacattca gatttagatg atattttagt aaatatgttc ttatatttac ctaatttttc
    12781 caaaatcaaa attctgaaga taatatgtat ctggctcaaa gaattatgta tcaagtagat
    12841 gatattttaa aagaagatat gctaaacgaa tattattact tacctaaaac actatataat
    12901 acattagcta gtccagagtt tgatgattta aagagaactg atgcaagtca agtcgatggg
    12961 caagatgata cttcagaaga tgatgataac gaaagtgaaa aagctgactc taaatctgca
    13021 gatagtgagt caaaaggtgg cgcctattta gaaatggagc ttcacgaagg acaaaatagt
    13081 gaaacattag gtaatgatga agcacgtgaa ggcgatgcta cagatgatat gactgatatg
    13141 atgaccaaaa agggcaaagg atcaaacgat acgctaaatc gtgaagaagg cgatgctgtt
    13201 ggtcaaagcc aagcatttca acttgatggt gtgaacaaaa atgtagaaat aaaatggcaa
    13261 attcctgaaa ttgagccaca atatgtttta gaatatcaag aatcgaaaca agacgtacaa
    13321 tatgaaatca aagacttaat tcaaattata aagaaacgat tgaacgagaa caacgagatg
    13381 cacgttttaa tttaacaaaa gggcgattac aaaaggattt aattaattgg tttatcgacg
    13441 atcaatataa attgttttac aaaaaacaag atttaagtaa atcatttgat gcgacattta
    13501 cattgttaat tgatgcgtca gcaagtatgc atgataaaat ggctgaaacg aagaaaggtg
    13561 tagtgttttc cacgagacgt taaaagcatt gaacattaaa catgaaattt tcattcagtg
    13621 aggatgcctt tgattcagac gagcatgcac aaccaaatat cattaatgaa attattaact
    13681 atgattactc aacctttgaa aaagatggcc cacgtattat ggcacttgaa cctcaagatg
    13741 ataatcgtga tggtgttgct att
//
