LOCUS       CP049562               13160 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt183 plasmid pER05008.3A.1, complete
            sequence.
ACCESSION   CP049562
VERSION     CP049562.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331000
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 226.750871717929x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 17:05:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,837
            CDSs (total)                      :: 2,756
            Genes (coding)                    :: 2,537
            CDSs (with protein)               :: 2,537
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 186 of 219
            Pseudo Genes (incomplete)         :: 22 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 9 of 219
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt183"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER05008.3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            join(13009..13160,1..466)
                     /gene="cadD"
                     /locus_tag="FPO46_14115"
     CDS             join(13009..13160,1..466)
                     /gene="cadD"
                     /locus_tag="FPO46_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="QNZ72506.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadD"
     gene            485..832
                     /locus_tag="FPO46_14120"
     CDS             485..832
                     /locus_tag="FPO46_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNZ72507.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            989..1081
                     /locus_tag="FPO46_14125"
     CDS             989..1081
                     /locus_tag="FPO46_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNZ72508.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1253..2224)
                     /locus_tag="FPO46_14130"
     CDS             complement(1253..2224)
                     /locus_tag="FPO46_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNZ72509.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            2348..2767
                     /locus_tag="FPO46_14135"
     CDS             2348..2767
                     /locus_tag="FPO46_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNZ72510.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(3293..4138)
                     /gene="blaZ"
                     /locus_tag="FPO46_14140"
     ARG             complement(3297..4138)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4245..6002
                     /gene="blaR1"
                     /locus_tag="FPO46_14145"
     ARG             4246..5999
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            5992..6372
                     /gene="blaI"
                     /locus_tag="FPO46_14150"
     ARG             5993..6369
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            6636..7214
                     /locus_tag="FPO46_14155"
     CDS             6636..7214
                     /locus_tag="FPO46_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNZ72514.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(7658..8317)
                     /locus_tag="FPO46_14160"
     CDS             complement(7658..8317)
                     /locus_tag="FPO46_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QNZ72515.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKKTFQLI"
                     /name="DUF536 domain-containing protein"
     gene            8742..9725
                     /locus_tag="FPO46_14165"
     CDS             8742..9725
                     /locus_tag="FPO46_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QNZ72516.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            9718..10034
                     /locus_tag="FPO46_14170"
                     /pseudo=""
     CDS             9718..10034
                     /locus_tag="FPO46_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            10581..10730
                     /locus_tag="FPO46_14175"
     CDS             10581..10730
                     /locus_tag="FPO46_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ72517.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(10898..11590)
                     /locus_tag="FPO46_14180"
     CDS             complement(10898..11590)
                     /locus_tag="FPO46_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ72518.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            11700..11894
                     /locus_tag="FPO46_14185"
     CDS             11700..11894
                     /locus_tag="FPO46_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ72519.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     ARG             complement(3294..4138)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          10091..10238
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(7576..8317)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8743..9725
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9857..10026
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 attagttagt ttgtttttag cttttgtatt aaattatgtt ccagaaaagt ggatattagg
       61 tttattgggt ttaataccaa tttatcttgg aattaaagtg gctatttatg atgattgtga
      121 aggagaaaag agagctaaaa aagaattgaa tgaaaaagga ttgtctaaat tagttggtac
      181 ggttgcaatt gttacgatag caagttgtgg cgccgataat attggtttat ttgttccgta
      241 ttttgtgaca ttaagtgtta ctaatttatt acttactctg tttgtctttt taattttaat
      301 tttcttcttg gtgtttactg cacaaaaatt agctaatatt ccaggaattg gagaaattgt
      361 tgagaaattt agtcgttgga ttatggctat tatttatata gctttaggtt tatttattat
      421 tattgaaaat gacactattc aaacaatttt aggatttata ttttaattta gggtgtgatt
      481 ttatatgagt tatgaaaata cttgtgatgt gatctgtgta catgaggata aagttaacaa
      541 tgctttaagt tttttagaag atgataaatc taagaaatta cttaacattt tagaaaaaat
      601 ttgtgatgag aagaaattga aaatcatatt atctttgatt aaagaagatg agttgtgtgt
      661 ttgtgatatt tctttgatat tgaaaatgag tgttgcttca acttcacatc atttaaggct
      721 tttatataaa aatgaggtac ttgattttta taaagatgga aagatggcat attattttat
      781 taaagacgat gaaataagag agtttttctc taaaaatcat gagggttttt gaaacgagtt
      841 tcttcttgtc ttgatactat aaaaaacaaa gttatataac ataagacgac ttatcggaag
      901 tagatatgct tgtatattca cagatggtat aataatagag tcgcctatct ctcaggcgtc
      961 aattgacgaa gagaggaggt gcattacttt gctaatcatt ttcgttcaca tcatagcacc
     1021 agtcatcagt ggctgtgctg ttgcgtatta tacttattgg cttagtaaac gcaacaaata
     1081 attaataggc gactttataa gccagcatcg aaaaaatccc cacactactg caatagtgtg
     1141 gggattggtg cattaattgt taatcatttt cgttatataa attataacac tggttagtag
     1201 agaaatgcaa aatgattgaa ttaatagtaa atgtattaat gaatactcac acttatactt
     1261 ttaaaatagt tcttcctaaa tgttgtcctt ttaaaagctg atctatcgtt ttatagaatt
     1321 cttcaaatcc aatttcattg tatagaagtg aatttcctat attccattca tttgctaact
     1381 tactccatat atcttcacgg atattatgat caacatatac agaatcaatg ccaattaatt
     1441 taatacctct aagaataaac ggtaatatat tagcgttaag tgaatttcct gctacatttc
     1501 cacatacagt agcaacacct tgataattta gacgttttaa accgtataat gttacattac
     1561 tccccactgt atctaagaaa taatcaaaaa tacctttttt aagcggttta ttatcatctt
     1621 caattattat tacttgtttt gcacctaatt tttttgcgat ttctatttgc ttttcttttc
     1681 ttacgatagc agtgatattt tcatatccaa ttttatgtaa aatttgtata gcaacactgc
     1741 ccacaccacc tgtagcccca gacactaaaa tttcaggttg tgatttgata ttcattccat
     1801 atttttctag ttcaaaaatt gataatgctg ccgtaaaacc ggctgtgcca tataccatag
     1861 actctttaat agacatttct tggggaatag gaacaatcca ctctccaggc acttgtgcat
     1921 attctgaaaa tccacctgta tgtgtcacgc caacatcata accagtaact aatacattat
     1981 cgccttcttt aaacttatca ttcgaacttg attcaacaat acctgcaaaa tctataccag
     2041 gaatcatagg ataatctcta attacaccgc ctttatattg taaagctagc atatccttat
     2101 aatttattga agagtatacc acttttacta atacatcacc cttcgataaa tgagattcat
     2161 caactttttc tactttataa ctaacatttg atgcactttt tctcactact accgcattga
     2221 acatttatta gccttctttc acaattgttt tgtagaagta tgtttttgtt gcaaaactat
     2281 tttcctacaa aacttatcat gatatgataa gcgagtcaat actaatgatt taagagaggt
     2341 tttagttgtg gaattagata attgtataaa ttatttgttg agtacttctc agaatagagt
     2401 ttttaaacat tttaaaagtt tattaagtcc ctataatatt actccagcag aatatggtgt
     2461 tttgaattgc ttgattaata gtaatttgaa tacacctaaa gcgattggta atactttaaa
     2521 tttagaacct tcaacaatat ctggaatatt agataagatg tccaaaaagg atctaataac
     2581 acgtcaaact gataatgaaa atagaagaac tgtgctaata aacagtacaa ataaagcaaa
     2641 agacttatgg ccaacattag aaaagaaagc ctatcaactt aataatcatt acttcgctaa
     2701 tttaactaat gaagaaactg tagtgtttaa aaagatatta ctaaagataa ataaaactac
     2761 gttttaattt ttacgttata aaaaagttga agtgcgaatt ataatcgagt aaatataaat
     2821 gatagcttag cttaattata tattaaagcc aactatactt ttattcccga aaaacctact
     2881 atccaaattt ctaagatact ttagcctcta taataaacat attgtaatat aatttatgta
     2941 ttatttaggt ttaccctaat cgaacaataa aaaatacttg taaaatgcta ttgtcacttt
     3001 gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata
     3061 agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat
     3121 gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga
     3181 aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa
     3241 aatataatac aaactataag atgttatcag tatttattat gcatttagaa tattaaaatt
     3301 ccttcattac actcttggcg gtttcactta tcaacttatc atttggctta tcacttttat
     3361 tgtctttatt cgtaaaaatg actaaaacaa taggttcaga ttggccctta ggataaacaa
     3421 aagcaacatc atttctagaa gcatatgtta ttgcttgacc acttttatca gcaaccttat
     3481 agtcttttgg aacaccatct ttaattaaag tgtctccgtt tttattatta aacattaaat
     3541 caagtaagaa atttttattt tttttgctta attttccatt tgcgataagt ttatttaaag
     3601 tcttgccgaa agcagcaggc gttgaagtat cttttttgct ctttggtgaa tagtaattta
     3661 attctatctc atatctaact ggatttgtta ctttatctcc tagttctttt agacgttgtt
     3721 taactttttt gattccaccg atttctttta taattttatt gtttgctgta ttatcactat
     3781 atgtcattga agcctcaata agttctttta aagtgatatc ttttcctaca tatttttcta
     3841 aaataggaga ataagcaact atatcatctt tgttaatatg tactttttta tttaacttat
     3901 tataaggtac ttgttctaac aaaatagcac tatttatcgc ttttgaagtc gaagcatagg
     3961 caaatctctt atctgaatta aattttactt ccttaccact tttagtattt aaagcataaa
     4021 caccaatatg agcattatat tttttttcta aatcatttaa ctctttggca tgtgaactgt
     4081 ttgaattaca tgcacttaaa actaaagcaa ttacaattaa aaatattaac tttttcaaaa
     4141 taaaccctcc gatattacag ttgtaatttt tataaatcaa taatattaca attgtaatat
     4201 tgatgtcaat tgtttattat aaatatttga aagaaggttt caaaatggct aaattactaa
     4261 taatgagcgt agtaagcttt tgttttatat ttctattgtt agtatttttt aggtacatat
     4321 taaaacgcta ttttaattat agtttaaatt ataaagtttg gtatctaact atgcttgctg
     4381 gattaattcc ttttatccca attaaattct cttttataaa atttaataat gtgaataatc
     4441 aagcgcccac agttgaaagt aagtcacacg atttgaacca taacataaat accaccaaac
     4501 ctattcaaga gttcacaaca gatatccata agtttaattg ggattcaatt gataatatct
     4561 gcacagttat ttggatagtt ttagttatta ttttaagttt taaatttttg aaagccttat
     4621 tatatcttaa atatttaaag aaacagtcac tttatctaaa cgaaaatgaa aaaaataaaa
     4681 tagatacgat acttttcaac catcaatata aaaaaaatat tgtgattcga aaagcagagg
     4741 ctattcaatc tccaataact ttttggtatg ggaaatatat tattttgatt cctagttcat
     4801 attttaaaag tgtaattgat aaaagactaa aatatataat actgcatgaa tatgctcatg
     4861 ctaaaaatag agatacttta catttaatta tctttaatat cttctctatt attatgagtt
     4921 acaatccatt agtacatatt gtaaaaagaa agataattca tgacaatgaa gtagaagccg
     4981 atagatttgt ccttaataat attaacaaaa atgaatttaa aacttatgcg gaatctatta
     5041 tggactccgt attaaaaacc ccattttcca acaaaaatat attaagccat tcatttaatg
     5101 gtaaaaaatc attactcaaa agtagattaa ttaatataaa agaagccgat ctgaaaaagc
     5161 agtcaaaatt aattctaatt tttatttgta tttttacttt ttttatcatg ataattcaaa
     5221 gccagttttt aatggggcaa tccttaactg attataatta taaaaagccc ttacaaagcg
     5281 attaccaaat tttagatgaa agtaaaaatt ttggttcaaa tagtggatct ttcgttatgt
     5341 acagcatgaa gaaagacaaa tactatattt ataatgaaaa agaaagtaga aaaagatatt
     5401 ctcctgattc aacttacaaa atttatttag cactgtttgg acttgaccgt catattataa
     5461 gtgataaaaa ttcacgtatg tcatggaatc ataaacatta tcttttcgag tcttggaata
     5521 aggaacaaga tttaaataca gcaatgcaaa attcagttaa ttggtacttc gaacgtatta
     5581 gtaatcaaat accaaagaac tatactgcgg ctcaactcaa gcaattaaat tatggtaatg
     5641 aaaatttggg aagttataaa agctattgga tggaagatag tttgaaaata tctaatcttg
     5701 aacaagtaat agtttttaaa aatatgatgg aacaaaataa ccattttagt aaaaaagcaa
     5761 agaatcaatt atcttcttca ttattgatta agaaaaatga aaagtatgaa ctgtatggga
     5821 aaacaggtac aggtatagta aacgggaagt ataataatgg gtggtttgta ggttacgtaa
     5881 ttacaaatca tgataagtat tattttgcta cacatttatc agatggaaac ccgtctggga
     5941 aaaatgctga attaataagt gaaaaaatat taaaaggaat gggtgtttta aatgaccaat
     6001 aagcaagttg aaatatctat ggctgaatgg gatgttatga atataatatg ggataaaaaa
     6061 tcagtatcag ctaatgaaat tgtagttgaa attcaaaaat ataaagaagt tagtgataaa
     6121 acgattagaa cattaatcac aagactatat aaaaaagaga ttataaaacg atacaaatca
     6181 gaaaatattt atttttactc atcaaatatt aaagaagacg atattaaaat gaaaactgct
     6241 aaaacctttc ttaataaact gtatggaggg gatatgaaaa gtttagtgct gaattttgcg
     6301 aaaaatgaag aattaaataa caaagaaatt gaagaattgc gagacatttt aaatgatatt
     6361 agtaaaaagt aattagaggt atgtttcaaa cataatacat atgtaaacca tacttgactc
     6421 ttattaaatt agttttgtta atgacattta aaatatatta ttaaagatga gagtgtccag
     6481 aaaataacca ttttctgtac actcttgttt tttgtctaaa taagtaagta ttacatagaa
     6541 ttatgtgttc aaaaacccaa caaaaaatct atgttcacaa aagacctcgt aatcaagtta
     6601 tggtacacta aaggtgtata taggaggtct gtattttgaa aataggttat gcaagagttt
     6661 caactggatt acaaaatttg aatttacagg aggatcgatt aaatacatat ggttgtgaaa
     6721 agatatttaa tgaccatatg agtggttcaa aaagtaagcg acctggttta gataaagcaa
     6781 ttgaatttgc gaggtcagga gatacgattg ttgtttggag attagataga ctaggacgga
     6841 acatggagga tttaatcacg ttagttaatg aacttaacga acgaggtgtg agttttcata
     6901 gtttagaaga aaatataacg atggataaat caagttctac aggacaatta ttatttcatt
     6961 tattcgcagc atttgcagag tttgaacgta atttaatttt agagcgatca tcagccggtc
     7021 gaattgcagc acgtgctaga ggccgatatg gaggtagacc tgaaaaatta aataaacaag
     7081 atttaaactt acttaagaca ctttatgata atggtacgcc aattaaaaca attgcagagc
     7141 agtggcaagt ttcacgtaca actatttatc gttatctcaa taaattagag gcaaaagaga
     7201 ataaaaacaa gtagagcaac taatctagct tactataatg aatctactaa ataattttat
     7261 atatcattaa catacaaaga ctttgattca atgtcactcg atgtaaaaga gacgttctga
     7321 cagcgtctaa aatctcataa tattttcctg ttatgagatt ttaataataa cacaagaaaa
     7381 aagcgtacaa attcgtacgc ttttttaata taatacctgt ttcactaatg aacgtttata
     7441 acgcaatcat gatgagagta ttaatacagt taattatttg aaatacttta gtttacataa
     7501 tcgatgatta ccagaagttc tcactatata aaagacacta aaattttata acttttagtg
     7561 cctaatcaat tattttagct accaaacaaa cgactccaaa accccttttt aggttccgtt
     7621 tcttcactct tttcttctat atcactatca ttgtcattta gattagctgg aacgtcttct
     7681 tttttgcttc ttcaccttgt gtactttcgt cgctttgact tccagatata tccttatatt
     7741 gaatctctgt ttctttagtt tcttcctgat ctctgttaat atttacagga ttagtagtat
     7801 atgttttttc ttgtgcatca acgttttgtc tagtgttagt agatgtatca aagggataat
     7861 taaggtgttt ttctttttct aattgatttt ctaatttttg aatcttttta ttactctcta
     7921 atgccagaac ttgctgattt tcaagcaatt ttgatctttc ttgcaactct tttctgtaaa
     7981 attcaattgt tgtttcatga cgactttctt gtttttcaat ttgattattt aaagtatcaa
     8041 tctgattttt aaggatttct actatttgag catcgctttt cgattcaaaa ttaatattat
     8101 ccgacccttt tttttgtttg tcttcagtag tactttcaaa accatatttc tttttatttt
     8161 catttactct ttgaatgatt ttttctatat cggtatcttc tttaataaac gaagtgtttt
     8221 cctgttttat tgtctctata ttcaatcgct taatattatt aaaaatcgtt tgcttactaa
     8281 cccctaattc ttcactcaac tttttcacac ttttcatatc atcaaccctt tataaaatgt
     8341 ttataaaccc ttgataaaat attataagct tataatgctt aataataaag tgattataaa
     8401 taaaaataaa catccatcaa cctttttatc aagggtttat gaaaggttta tgatatttat
     8461 caagggttta taaacccttg ataaagctat attttccctt ttataagttt atttaaagtg
     8521 ctataataaa cttaatataa caaaaacccc aactactggt aatagtcggg gtttggtaat
     8581 aaaacgttta gaaactgttt atttatataa ttttcgatac ttgtattata taatgaataa
     8641 tatcttagtg caagcaccta aatcattaaa taaatgtaaa gctaaataaa aacgttttat
     8701 tacctcttac caaactaaaa tatggaaaga ggttttttgt catgcaaaat caatatttta
     8761 cagtgcaaga aaattacaaa gaaagatttt atcaaatccc taaagtattc tttaccagtg
     8821 aaaattataa aaatctaact aatgatatga aaatagccta tgctatctta cgtgatcgct
     8881 taaatttatc tattaaaaat agctgggttg atgaagatgg taacatttac tttgtttatt
     8941 caaatgaaaa gctaatggaa atcttaaatt gtaaaaaaga aaagttaaca aaaataaaaa
     9001 aagggttaga aaacgatggt ctactcattc aaaaacgacg tggtttaaat aaaccaaata
     9061 ttctatattt aatgaaacct attgtcacag aacgtgatat ttacaaaata gaaaaagaag
     9121 aaaacgatgt tgaaccttac ggcgagaagg aagttcgaaa atcgaacgtc cagaagttcg
     9181 aaaatcgaac gtccagaagt tcgaaaatcg aacgtccaga agttcgaaaa tcgaacacta
     9241 atgatactga ctttaatgac attgacttta atgatactga gaataatgat atgaatgact
     9301 tgaatgatat aaagtacaaa aatgaaatta gtaatcattc aaatcattca aatcaattta
     9361 cacataattt tgatgataaa gaaatgctat tgcaagaatt cccagaacaa ctaactaatt
     9421 atttattaaa atatgattat agagatttag aaatcataaa ggctgttatt cttaaagcaa
     9481 aaaaatcatt taattcaagg catgaagata tgcattacat gttagaggac attgaggatg
     9541 aaatattaac ttctttaaaa agacttaaaa aagctataca tgatagggga gttaaaggcc
     9601 aaaaggaaac aatcaagtca atgcaagctt atttaatgca aacaattcta actgaacttg
     9661 aagaaacaca tgccctttat atgcgccgta aaaacatgaa acaatataat atctttaatc
     9721 aataattcaa atgattttat aacactcgaa gagacatcaa aatattccgt taaatcatct
     9781 gactttttaa ctgatgacga cgaaaaaaat caagaaattg taagtgattt agaaaaaggt
     9841 ttgtatcgaa aacgtatgtt aagttatggt ggattgctta aacaaaagca taaaatttta
     9901 aacttagacg atgccgaaga tggtaatttg attaatacaa gtgatgaaga taaaacgaca
     9961 gacgaagaaa aaaagcacat tcaattaccg caatttggaa tttcgaaaag caaaattatt
    10021 atttaagaca ttagtgataa ctgatgtctt tttttattgt ttttctgtaa aaaagtgctg
    10081 tcttattttt gtgacaaatg ctgtatgtag tgtcacaaaa gtgtgacact acagctttgt
    10141 atgatatcac tttaaaataa cttaaaaccc ttggaatgtc tggccttgcc agatctatca
    10201 tttttgaatg atagcaaatt tcccttatgc tcttacggag attttagagt taaattaaaa
    10261 tttctaagtt tttgataaga aaagccctgc aggaatttag aaaaacatgg ggaagtttta
    10321 aagtatttta tgctaatttt taaatttgca tgtaaatggg cagtatctaa aaaattagtc
    10381 actgattttg ctgaaatttt tgtttgtgaa attagcatat atttatttgg ctcatatttg
    10441 cgttttaaga gactgtatag atttttaagt acaaaaccta tatgatttgc ctctaagtct
    10501 tcaaaatgcc tcttaaaatc caaaataaaa gtgtttaata ttgtaataag gattgctatt
    10561 tttataaaag gagagatatc atgaagatta aaactttcgc tacttttact catcgtcaat
    10621 tagataaaca agttaatagg tttttaaaga atgatattca tgtattagaa attcaaacac
    10681 acagtaattg gttatatatt tcagctattg ttttttatga ggataaataa ggaacatcgt
    10741 atttatgccg aaaaaattta ttgatgttga gaagaaccct taactaaact tgcagacgaa
    10801 tgtcggcata gcgtgagcta ttaagccgac cattcgacaa gttttgggat tgttaagggt
    10861 tccgaggctc aacgtcaata aaacaattgg aataaaatca acctacagag catccagctg
    10921 caaatgcagt tccgcaaact gcttcacata caccgccggc tattggattt acgattgccc
    10981 atactccaca gtagtgagca catgcataaa gtccagccat actacaagcg aatgctttgc
    11041 cgttccattt gaatcctgca tatgttaccg gtctgttttt atcaactttt tcagccttat
    11101 attcaacgta atttttgtta ataagtattt ttccctcttt gttttcagtc atacttcttt
    11161 tttcggcaat gaattttgtg atttttttgt tgtagttatc atatattgtt tcagaaacta
    11221 caatttcttt tgttttttca tttttataag cgttattagc tatgtagcca tatgcatttt
    11281 tatcatttgc ttctgtagct agatatttta atttagaatt tttgtcttct agctttattt
    11341 ttttaaatcc ttcttttttc atttgctttt cgaatttttg catatcttca tttttatttt
    11401 gcaattgttc tagaaaactt atttctttct tggtcatttg tgatacttcg atgtttttgc
    11461 ttttcaattt ttctttttga ttttctagta aagaactttt ttgttcttca tttgcgtaag
    11521 cattagaaaa attcgcgcta aataccagta ggaacaagga aattattgaa aataatttta
    11581 taattttcat ttgatacctc caagataata agatatttta attgtataag taagcgcttt
    11641 ataaaatcaa tatttatttt aatatattta taaacatatt tttgaaagga gtctatttta
    11701 tgaatgaaaa tttagaaaaa tatataaaaa gtttgccact tttaggtttg ttaattagta
    11761 ttttgttaat tgttttattc ttttttattt tagatgttga tgaagattta ttcgtggttt
    11821 ttttatactg tttattgcct ttgatagtta atttgtctat ttatggtttt tatgtttttg
    11881 ttaaaagaaa gtaagattat gaataatttt gtttttaatt tgtatgcttt gagggggttg
    11941 gaaaacggta ggtgtataga ttagatttct tgtttttttg aaacgagtgt gaacgagttt
    12001 cttcttgtct tgatttttga taaaaacgcc ctacaggaat ttagaaaaac gtggggaagt
    12061 tttaaagaat tttatgctaa tttttaattt gcatgtaaat gggcagtgtc taaaaatagt
    12121 tattgttttt gctcaaattt ttgtttttga aaatagcata tatttatttg gctcatattt
    12181 gcgttttaaa agcatatata agttttagat ataaaactat atgaatcacc cctaaatctt
    12241 taaaatgccc tcttaaaatt caaaataagg gcataaaaaa taaaagctac cccagataaa
    12301 gtgaggtagc tttttctttc atacggtcta tttatttttc tttcgacgcc gtatcgtcga
    12361 tgtaattcaa aaaagttatg ggctataaat ctacatcact ttttcaagaa tgtagtagca
    12421 atattcaacg caaattaatt tttataactg ctcggaatat ctcaagccgt ttctttaatt
    12481 ttgaaataaa aaaatcgacg aaggtcgatt acgtttttgc acatgtcgat ttatccgacg
    12541 tataagtgcg cccttacggg atttaactag attataacga cgaattttag acctgtaaag
    12601 caataacaat taggtctacc aggtagataa agtgtacaaa gtagaaccga aaaagttatt
    12661 tattttgaag atttttcgta gatataattt gctaataaac ttcctaaaac tgaataaaga
    12721 aaaatagtta aactcatgta atcactccat aattctgaag tgtctccatg tctacgcata
    12781 gacacgcatt tatgccgaaa aaatttattg atgttgagaa gaacccttaa ctaaacttgc
    12841 agacgaatgt cggcatagcg tgagctatta agccgaccat tcgacaagtt ttggattgtt
    12901 aagggttccg aggctcaacg tcaataaagc aattggaata aagcacctat gattatttaa
    12961 aaatataagt tataatacat tcaaaaatat ttttgaatga ggtgtattat gattcaaacg
    13021 gttgttgctg ctgctgttct ttatattgct acagcagttg atttattagt aattttatta
    13081 atattttttg ctagagcaaa gactagaaaa gaatatagag atatttatgt tggtcaatat
    13141 ttagggtcta ttattttaat
//
