LOCUS       CP049549               13163 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt193 plasmid pER05282.3A.2, complete
            sequence.
ACCESSION   CP049549
VERSION     CP049549.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331010
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 369.149807881774x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:48:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,882
            CDSs (total)                      :: 2,800
            Genes (coding)                    :: 2,731
            CDSs (with protein)               :: 2,731
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt193"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER05282.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            complement(join(13015..13163,1..46))
                     /locus_tag="FPO56_00185"
     CDS             complement(join(13015..13163,1..46))
                     /locus_tag="FPO56_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ53597.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            156..848
                     /locus_tag="FPO56_00190"
     CDS             156..848
                     /locus_tag="FPO56_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ53584.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            complement(1016..1165)
                     /locus_tag="FPO56_00195"
     CDS             complement(1016..1165)
                     /locus_tag="FPO56_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ53585.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(1712..2028)
                     /locus_tag="FPO56_00200"
                     /pseudo=""
     CDS             complement(1712..2028)
                     /locus_tag="FPO56_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(2021..3004)
                     /locus_tag="FPO56_00205"
     CDS             complement(2021..3004)
                     /locus_tag="FPO56_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QNZ53586.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            3430..4173
                     /locus_tag="FPO56_00210"
     CDS             3430..4173
                     /locus_tag="FPO56_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QNZ53587.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(4534..5112)
                     /locus_tag="FPO56_00215"
     CDS             complement(4534..5112)
                     /locus_tag="FPO56_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNZ53588.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(5376..5756)
                     /gene="blaI"
                     /locus_tag="FPO56_00220"
     ARG             complement(5380..5756)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(5746..7503)
                     /gene="blaR1"
                     /locus_tag="FPO56_00225"
     ARG             complement(5750..7503)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            7610..8455
                     /gene="blaZ"
                     /locus_tag="FPO56_00230"
     ARG             7611..8452
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8981..9400)
                     /locus_tag="FPO56_00235"
     CDS             complement(8981..9400)
                     /locus_tag="FPO56_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNZ53592.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            9524..10495
                     /locus_tag="FPO56_00240"
     CDS             9524..10495
                     /locus_tag="FPO56_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNZ53593.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(10667..10759)
                     /locus_tag="FPO56_00245"
     CDS             complement(10667..10759)
                     /locus_tag="FPO56_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNZ53594.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10916..11263)
                     /locus_tag="FPO56_00250"
     CDS             complement(10916..11263)
                     /locus_tag="FPO56_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNZ53595.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(11282..11899)
                     /gene="cadD"
                     /locus_tag="FPO56_00255"
     ARG             complement(11286..11899)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7611..8455
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(1509..1656)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(1715..1890)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(2022..3004)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3431..4173
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaaaagtgg caaacttttt atatattttt ctaaattttc attcataaaa tagactcctt
       61 tcaaaaatat gtttataaat atattaaaat aaatattgat tttataaagc gcttacttat
      121 acaattaaaa tatcttatta tcttggaggt atcaaatgaa aattataaaa ttattttcaa
      181 taatttcctt gttcctactg gtatttagcg cgaatttttc taatgcttac gcaaatgaag
      241 aacaaaaaag ttctttacta gaaaatcaaa aagaaaaatt gaaaagcaaa aacatcgaag
      301 tatcacaaat gaccaagaaa gaaataagtt ttctagaaca attgcaaaat aaaaatgaag
      361 atatgcaaaa attcgaaaag caaatgaaaa aagaaggatt taaaaaaata aagctagaag
      421 acaaaaattc taaattaaaa tatctagcta cagaagcaaa tgataaaaat gcatatggct
      481 acatagctaa taacgcttat aaaaatgaaa aaacaaaaga aattgtagtt tctgaaacaa
      541 tatatgataa ctacaacaaa aaaatcacaa aattcattgc cgaaaaaaga agtatgactg
      601 aaaacaaaga gggaaaaata cttattaaca aaaattacgt tgaatataag gctgaaaaag
      661 ttgataaaaa cagaccggta acatatgcag gattcaaatg gaacggcaaa gcattcgctt
      721 gtagtatggc tggactttat gcatgtgctc actactgtgg agtatgggca atcgtaaatc
      781 caatagccgg cggtgtatgt gaagcagttt gcggaactgc atttgcagct ggatgctctg
      841 taggttgatt ttattccaat tgttttattg acgttgagcc tcggaaccct taacaatccc
      901 aaaacttgtc gaatggtcgg cttaatagct cacgctatgc cgacattcgt ctgcaagttt
      961 agttaagggt tcttctcaac atcaataaat tttttcggca taaatacgat gttccttatt
     1021 tatcctcata aaaaacaata gctgaaatat ataaccaatt actgtgtgtt tgaatttcta
     1081 atacatgaat atcattcttt aaaaacctat taacttgttt atctaattga cgatgagtaa
     1141 aagtagcgaa agttttaatc ttcatgatat ctctcctttt ataaaaatag caatccttat
     1201 tacaatatta aacactttta ttttggattt taagaggcat tttgaagact tagaggcaaa
     1261 tcatataggt tttgtactta aaaatctata cagtctctta aaacgcaaat atgagccaaa
     1321 taaatatatg ctaatttcac aaacaaaaat ttcagcaaaa tcagtgacta attttttaga
     1381 tactgcccat ttacatgcaa atttaaaaat tagcataaaa tactttaaaa cttccccatg
     1441 tttttctaaa ttcctgcagg gcttttctta tcaaaaactt agaaatttta atttaactct
     1501 aaaatctccg taagagcata agggaaattt gctatcattc aaaaatgata gatctggcaa
     1561 ggccagacat tccaagggtt ttaagttatt ttaaagtgat atcatacaaa gctgtagtgt
     1621 cacacttttg tgacactaca tacagcattt gtcacaaaaa taagacagca cttttttaca
     1681 gaaaaacaat aaaaaaagac atcagttatc actaatgtct taaataataa ttttgctttt
     1741 cgaaattcca aattgcggta attgaatgtg ctttttttct tcgtctgtcg ttttatcttc
     1801 atcacttgta ttaatcaaat taccatcttc ggcatcgtct aagtttaaaa ttttatgctt
     1861 ttgtttaagc aatccaccat aacttaacat acgttttcga tacaaacctt tttctaaatc
     1921 acttacaatt tcttgatttt tttcgtcgtc atcagttaaa aagtcagatg atttaacgga
     1981 atattttgat gtctcttcga gtgttataaa atcatttgaa ttattgatta aagatattat
     2041 attgtttcat gtttttacgg cgcatataaa gggcatgtgt ttcttcaagt tcagttagaa
     2101 ttgtttgcat taaataagct tgcattgact tgattgtttc cttttggcct ttaactcccc
     2161 tatcatgtat agctttttta agtcttttta aagaagttaa tatttcatcc tcaatgtcct
     2221 ctaacatgta atgcatatct tcatgccttg aattaaatga tttttttgct ttaagaataa
     2281 cagcctttat gatttctaaa tctctataat catattttaa taaataatta gttagttgtt
     2341 ctgggaattc ttgcaatagc atttctttat catcaaaatt atgtgtaaat tgatttgaat
     2401 gatttgaatg attactaatt tcatttttgt actttatatc attcaagtca ttcatatcat
     2461 tattctcagt atcattaaag tcaatgtcat taaagtcagt atcattagtg ttcgattttc
     2521 gaacttctgg acgttcgatt ttcgaacttc tggacgttcg attttcgaac ttctggacgt
     2581 tcgattttcg aacttccttc tcgccgtaag gttcaacatc gttttcttct ttttctattt
     2641 tgtaaatatc acgttctgtg acaataggtt tcattaaata tagaatattt ggtttattta
     2701 aaccacgtcg tttttgaatg agtagaccat cgttttctaa cccttttttt atttttgtta
     2761 acttttcttt tttacaattt aagatttcca ttagcttttc atttgaataa acaaagtaaa
     2821 tgttaccatc ttcatcaacc cagctatttt taatagataa atttaagcga tcacgtaaga
     2881 tagcataggc tattttcata tcattagtta gatttttata attttcactg gtaaagaata
     2941 ctttagggat ttgataaaat ctttctttgt aattttcttg cactgtaaaa tattgatttt
     3001 gcatgacaaa aaacctcttt ccatatttta gtttggtaag aggtaataaa acgtttttat
     3061 ttagctttac atttatttaa tgatttaggt gcttgcacta agatattatt cattatataa
     3121 tacaagtatc gaaaattata taaataaaca gtttctaaac gttttattac caaaccccga
     3181 ctattaccag tagttggggt ttttgttata ttaagtttat tatagcactt taaataaact
     3241 tataaaaggg aaaatatagc tttatcaagg gtttataaac ccttgataaa tatcataaac
     3301 ctttcataaa cccttgataa aaaggttgat ggatgtttat ttttatttat taatcacttt
     3361 attattaagc attataagct tataatattt tatcaagggt ttataaacat tttataaagg
     3421 gttgatgata tgaaaagtgt gaaaaagttg agtgaagaat taggggttag taagcaaacg
     3481 atttttaata atattaagcg attgaatata gagacaataa aacaggaaaa cacttcgttt
     3541 attaaagaag ataccgatat agaaaaaatc attcaaagag taaatgaaaa taaaaagaaa
     3601 tatggttttg aaagtactac tgaagacaaa caaaaaaaag ggtcggataa tattaatttt
     3661 gaatcgaaaa gcgatgctca aatagtagaa atccttaaaa atcagattga tactttaaat
     3721 aatcaaattg aaaaacaaga aagtcgtcat gaaacaacaa ttgaatttta cagaaaagag
     3781 ttgcaagaaa gatcaaaatt gcttgaaaat cagcaagttc tggcattaga gagtaataaa
     3841 aagattcaaa aattagaaaa tcaattagaa aaagaaaaac accttaatta tccctttgat
     3901 acatctacta acactagaca aaacgttgat gcacaagaaa aaacatatac tactaatcct
     3961 gtaaatatta acagagatca ggaagaaact aaagaaacag agattcaata taaggatata
     4021 tctggaagtc aaagcgacga aagtacacaa ggtgaagaag caaaaaaaga agacgttcca
     4081 gctaatctaa atgacaatga tagtgatata gaagaaaaga gtgaagaaac ggaacctaaa
     4141 aaggggtttt ggagtcgttt gtttggtagc taaaataatt gattaggcac taaaagttat
     4201 aaaattttag tgtcttttat atagtgagaa cttctggtaa tcatcgatta tgtaaactaa
     4261 agtatttcaa ataattaact gtattaatac tctcatcatg attgcgttat aaacgttcat
     4321 tagtgaaaca ggtattatat taaaaaagcg tacgaatttg tacgcttttt tcttgtgtta
     4381 ttattaaaat ctcataacag gaaaatatta tgagatttta gacgctgtca gaacgtctct
     4441 tttacatcga gtgacattga atcaaagtct ttgtatgtta atgatatata aaattattta
     4501 gtagattcat tatagtaagc tagattagtt gctctacttg tttttattct cttttgcctc
     4561 taatttattg agataacgat aaatagttgt acgtgaaact tgccactgct ctgcaattgt
     4621 tttaattggc gtaccattat cataaagtgt cttaagtaag tttaaatctt gtttatttaa
     4681 tttttcaggt ctacctccat atcggcctct agcacgtgct gcaattcgac cggctgatga
     4741 tcgctctaaa attaaattac gttcaaactc tgcaaatgct gcgaataaat gaaataataa
     4801 ttgtcctgta gaacttgatt tatccatcgt tatattttct tctaaactat gaaaactcac
     4861 acctcgttcg ttaagttcat taactaacgt gattaaatcc tccatgttcc gtcctagtct
     4921 atctaatctc caaacaacaa tcgtatctcc tgacctcgca aattcaattg ctttatctaa
     4981 accaggtcgc ttactttttg aaccactcat atggtcatta aatatctttt cacaaccata
     5041 tgtatttaat cgatcctcct gtaaattcaa attttgtaat ccagttgaaa ctcttgcata
     5101 acctattttc aaaatacaga cctcctatat acacctttag tgtaccataa cttgattacg
     5161 aggtcttttg tgaacataga ttttttgttg ggtttttgaa cacataattc tatgtaatac
     5221 ttacttattt agacaaaaaa caagagtgta cagaaaatgg ttattttctg gacactctca
     5281 tctttaataa tatattttaa atgtcattaa caaaactaat ttaataagag tcaagtatgg
     5341 tttacatatg tattatgttt gaaacatacc tctaattact ttttactaat atcatttaaa
     5401 atgtctcgca attcttcaat ttctttgtta tttaattctt catttttcgc aaaattcagc
     5461 actaaacttt tcatatcccc tccatacagt ttattaagaa aggttttagc agttttcatt
     5521 ttaatatcgt cttctttaat atttgatgag taaaaataaa tattttctga tttgtatcgt
     5581 tttataatct cttttttata tagtcttgtg attaatgttc taatcgtttt atcactaact
     5641 tctttatatt tttgaatttc aactacaatt tcattagctg atactgattt tttatcccat
     5701 attatattca taacatccca ttcagccata gatatttcaa cttgcttatt ggtcatttaa
     5761 aacacccatt ccttttaata ttttttcact tattaattca gcatttttcc cagacgggtt
     5821 tccatctgat aaatgtgtag caaaataata cttatcatga tttgtaatta cgtaacctac
     5881 aaaccaccca ttattatact tcccgtttac tatacctgta cctgttttcc catacagttc
     5941 atacttttca tttttcttaa tcaataatga agaagataat tgattctttg cttttttact
     6001 aaaatggtta ttttgttcca tcatattttt aaaaactatt acttgttcaa gattagatat
     6061 tttcaaacta tcttccatcc aatagctttt ataacttccc aaattttcat taccataatt
     6121 taattgcttg agttgagccg cagtatagtt ctttggtatt tgattactaa tacgttcgaa
     6181 gtaccaatta actgaatttt gcattgctgt atttaaatct tgttccttat tccaagactc
     6241 gaaaagataa tgtttatgat tccatgacat acgtgaattt ttatcactta taatatgacg
     6301 gtcaagtcca aacagtgcta aataaatttt gtaagttgaa tcaggagaat atctttttct
     6361 actttctttt tcattataaa tatagtattt gtctttcttc atgctgtaca taacgaaaga
     6421 tccactattt gaaccaaaat ttttactttc atctaaaatt tggtaatcgc tttgtaaggg
     6481 ctttttataa ttataatcag ttaaggattg ccccattaaa aactggcttt gaattatcat
     6541 gataaaaaaa gtaaaaatac aaataaaaat tagaattaat tttgactgct ttttcagatc
     6601 ggcttctttt atattaatta atctactttt gagtaatgat tttttaccat taaatgaatg
     6661 gcttaatata tttttgttgg aaaatggggt ttttaatacg gagtccataa tagattccgc
     6721 ataagtttta aattcatttt tgttaatatt attaaggaca aatctatcgg cttctacttc
     6781 attgtcatga attatctttc tttttacaat atgtactaat ggattgtaac tcataataat
     6841 agagaagata ttaaagataa ttaaatgtaa agtatctcta tttttagcat gagcatattc
     6901 atgcagtatt atatatttta gtcttttatc aattacactt ttaaaatatg aactaggaat
     6961 caaaataata tatttcccat accaaaaagt tattggagat tgaatagcct ctgcttttcg
     7021 aatcacaata ttttttttat attgatggtt gaaaagtatc gtatctattt tatttttttc
     7081 attttcgttt agataaagtg actgtttctt taaatattta agatataata aggctttcaa
     7141 aaatttaaaa cttaaaataa taactaaaac tatccaaata actgtgcaga tattatcaat
     7201 tgaatcccaa ttaaacttat ggatatctgt tgtgaactct tgaataggtt tggtggtatt
     7261 tatgttatgg ttcaaatcgt gtgacttact ttcaactgtg ggcgcttgat tattcacatt
     7321 attaaatttt ataaaagaga atttaattgg gataaaagga attaatccag caagcatagt
     7381 tagataccaa actttataat ttaaactata attaaaatag cgttttaata tgtacctaaa
     7441 aaatactaac aatagaaata taaaacaaaa gcttactacg ctcattatta gtaatttagc
     7501 cattttgaaa ccttctttca aatatttata ataaacaatt gacatcaata ttacaattgt
     7561 aatattattg atttataaaa attacaactg taatatcgga gggtttattt tgaaaaagtt
     7621 aatattttta attgtaattg ctttagtttt aagtgcatgt aattcaaaca gttcacatgc
     7681 caaagagtta aatgatttag aaaaaaaata taatgctcat attggtgttt atgctttaaa
     7741 tactaaaagt ggtaaggaag taaaatttaa ttcagataag agatttgcct atgcttcgac
     7801 ttcaaaagcg ataaatagtg ctattttgtt agaacaagta ccttataata agttaaataa
     7861 aaaagtacat attaacaaag atgatatagt tgcttattct cctattttag aaaaatatgt
     7921 aggaaaagat atcactttaa aagaacttat tgaggcttca atgacatata gtgataatac
     7981 agcaaacaat aaaattataa aagaaatcgg tggaatcaaa aaagttaaac aacgtctaaa
     8041 agaactagga gataaagtaa caaatccagt tagatatgag atagaattaa attactattc
     8101 accaaagagc aaaaaagata cttcaacgcc tgctgctttc ggcaagactt taaataaact
     8161 tatcgcaaat ggaaaattaa gcaaaaaaaa taaaaatttc ttacttgatt taatgtttaa
     8221 taataaaaac ggagacactt taattaaaga tggtgttcca aaagactata aggttgctga
     8281 taaaagtggt caagcaataa catatgcttc tagaaatgat gttgcttttg tttatcctaa
     8341 gggccaatct gaacctattg ttttagtcat ttttacgaat aaagacaata aaagtgataa
     8401 gccaaatgat aagttgataa gtgaaaccgc caagagtgta atgaaggaat tttaatattc
     8461 taaatgcata ataaatactg ataacatctt atagtttgta ttatattttg tattatcgtt
     8521 gacatgtata attttgatat caaaaactga ttttcccttt attattttcg agatttattt
     8581 tcttaattct ctttaacaaa ctagaaatat tgtatataca aaaaatcata aataatagat
     8641 gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg
     8701 ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa gtgacaatag
     8761 cattttacaa gtatttttta ttgttcgatt agggtaaacc taaataatac ataaattata
     8821 ttacaatatg tttattatag aggctaaagt atcttagaaa tttggatagt aggtttttcg
     8881 ggaataaaag tatagttggc tttaatatat aattaagcta agctatcatt tatatttact
     8941 cgattataat tcgcacttca acttttttat aacgtaaaaa ttaaaacgta gttttattta
     9001 tctttagtaa tatcttttta aacactacag tttcttcatt agttaaatta gcgaagtaat
     9061 gattattaag ttgataggct ttcttttcta atgttggcca taagtctttt gctttatttg
     9121 tactgtttat tagcacagtt cttctatttt cattatcagt ttgacgtgtt attagatcct
     9181 ttttggacat cttatctaat attccagata ttgttgaagg ttctaaattt aaagtattac
     9241 caatcgcttt aggtgtattc aaattactat taatcaagca attcaaaaca ccatattctg
     9301 ctggagtaat attataggga cttaataaac ttttaaaatg tttaaaaact ctattctgag
     9361 aagtactcaa caaataattt atacaattat ctaattccac aactaaaacc tctcttaaat
     9421 cattagtatt gactcgctta tcatatcatg ataagttttg taggaaaata gttttgcaac
     9481 aaaaacatac ttctacaaaa caattgtgaa agaaggctaa taaatgttca atgcggtagt
     9541 agtgagaaaa agtgcatcaa atgttagtta taaagtagaa aaagttgatg aatctcattt
     9601 atcgaagggt gatgtattag taaaagtggt atactcttca ataaattata aggatatgct
     9661 agctttacaa tataaaggcg gtgtaattag agattatcct atgattcctg gtatagattt
     9721 tgcaggtatt gttgaatcaa gttcgaatga taagtttaaa gaaggcgata atgtattagt
     9781 tactggttat gatgttggcg tgacacatac aggtggattt tcagaatatg cacaagtgcc
     9841 tggagagtgg attgttccta ttccccaaga aatgtctatt aaagagtcta tggtatatgg
     9901 cacagccggt tttacggcag cattatcaat ttttgaacta gaaaaatatg gaatgaatat
     9961 caaatcacaa cctgaaattt tagtgtctgg ggctacaggt ggtgtgggca gtgttgctat
    10021 acaaatttta cataaaattg gatatgaaaa tatcactgct atcgtaagaa aagaaaagca
    10081 aatagaaatc gcaaaaaaat taggtgcaaa acaagtaata ataattgaag atgataataa
    10141 accgcttaaa aaaggtattt ttgattattt cttagataca gtggggagta atgtaacatt
    10201 atacggttta aaacgtctaa attatcaagg tgttgctact gtatgtggaa atgtagcagg
    10261 aaattcactt aacgctaata tattaccgtt tattcttaga ggtattaaat taattggcat
    10321 tgattctgta tatgttgatc ataatatccg tgaagatata tggagtaagt tagcaaatga
    10381 atggaatata ggaaattcac ttctatacaa tgaaattgga tttgaagaat tctataaaac
    10441 gatagatcag cttttaaaag gacaacattt aggaagaact attttaaaag tataagtgtg
    10501 agtattcatt aatacattta ctattaattc aatcattttg catttctcta ctaaccagtg
    10561 ttataattta tataacgaaa atgattaaca attaatgcac caatccccac actattgcag
    10621 tagtgtgggg attttttcga tgctggctta taaagtcgcc tattaattat ttgttgcgtt
    10681 tactaagcca ataagtataa tacgcaacag cacagccact gatgactggt gctatgatgt
    10741 gaacgaaaat gattagcaaa gtaatgcacc tcctctcttc gtcaattgac gcctgagaga
    10801 taggcgactc tattattata ccatctgtga atatacaagc atatctactt ccgataagtc
    10861 gtcttatgtt atataacttt gttttttata gtatcaagac aagaagaaac tcgtttcaaa
    10921 aaccctcatg atttttagag aaaaactctc ttatttcatc gtctttaata aaataatatg
    10981 ccatctttcc atctttataa aaatcaagta cctcattttt atataaaagc cttaaatgat
    11041 gtgaagttga agcaacactc attttcaata tcaaagaaat atcacaaaca cacaactcat
    11101 cttctttaat caaagataat atgattttca atttcttctc atcacaaatt ttttctaaaa
    11161 tgttaagtaa tttcttagat ttatcatctt ctaaaaaact taaagcattg ttaactttat
    11221 cctcatgtac acagatcaca tcacaagtat tttcataact catataaaat cacaccctaa
    11281 attaaaatat aaatcctaaa attgtttgaa tagtgtcatt ttcaataata ataaataaac
    11341 ctaaagctat ataaataata gccataatcc aacgactaaa tttctcaaca atttctccaa
    11401 ttcctggaat attagctaat ttttgtgcag taaacaccaa gaagaaaatt aaaattaaaa
    11461 agacaaacag agtaagtaat aaattagtaa cacttaatgt cacaaaatac ggaacaaata
    11521 aaccaatatt atcggcgcca caacttgcta tcgtaacaat tgcaaccgta ccaactaatt
    11581 tagacaatcc tttttcattc aattcttttt tagctctctt ttctccttca caatcatcat
    11641 aaatagccac tttaattcca agataaattg gtattaaacc caataaacct aatatccact
    11701 tttctggaac ataatttaat acaaaagcta aaaacaaact aactaatatt aaaataatag
    11761 accctaaata ttgaccaaca taaatatctc tatattcttt tctagtcttt gctctagcaa
    11821 aaaatattaa taaaattact aataaatcaa ctgctgtagc aatataaaga acagcagcag
    11881 caacaaccgt ttgaatcata atacacctca ttcaaaaata tttttgaatg tattataact
    11941 tatattttta aataatcata ggtgctttat tccaattgct ttattgacgt tgagcctcgg
    12001 aacccttaac aatcccaaaa cttgtcgaat ggtcggctta atagctcacg ctatgccgac
    12061 attcgtctgc aagtttagtt aagggttctt ctcaacatca ataaattttt tcggcataaa
    12121 tgcgtgtcta tgcgtagaca tggagacact tcagaattat ggagtgatta catgagttta
    12181 actatttttc tttattcagt tttaggaagt ttattagcaa attatatcta cgaaaaatct
    12241 tcaaaataaa taactttttc ggttctactt tgtacacttt atctacctgg tagacctaat
    12301 tgttattgct ttacaggtct aaaattcgtc gttataatct agttaaatcc cgtaagggcg
    12361 cacttatacg tcggataaat cgacatgtgc aaaaacgtaa tcgaccttcg tcgatttttt
    12421 tatttcaaaa ttaaagaaac ggcttgagat attccgagca gttataaaaa ttaatttgcg
    12481 ttgaatattg ctactacatt cttgaaaaag tgatgtagat ttatagccca taactttttt
    12541 gaattacatc gacgatacgg cgtcgaaaga aaaataaata gaccgtatga aagaaaaagc
    12601 tacctcactt tatctggggt agcttttatt ttttatgccc ttattttgaa ttttaagagg
    12661 gcattttaaa gatttagggg tgattcatat agttttatat ctaaaactta tatatgcttt
    12721 taaaacgcaa atatgagcca aataaatata tgctattttc aaaaacaaaa atttgagcaa
    12781 aaacaataac tatttttaga cactgcccat ttacatgcaa attaaaaatt agcataaaat
    12841 tctttaaaac ttccccacgt ttttctaaat tcctgtaggg cgtttttatc aaaaatcaag
    12901 acaagaagaa actcgttcac actcgtttca aaaaaacaag aaatctaatc tatacaccta
    12961 ccgttttcca accccctcaa agcatacaaa ttaaaaacaa aattattcat aatcttactt
    13021 tcttttaaca aaaacataaa aaccataaat agacaaatta actatcaaag gcaataaaca
    13081 gtataaaaaa accacgaata aatcttcatc aacatctaaa ataaaaaaga ataaaacaat
    13141 taacaaaata ctaattaaca aac
//
