LOCUS       CP049533               13159 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt205 plasmid pER05509.3A.2, complete
            sequence.
ACCESSION   CP049533
VERSION     CP049533.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331022
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13159)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 291.221384887006x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:47:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,807
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 43 of 69
            Pseudo Genes (incomplete)         :: 26 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt205"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER05509.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            complement(join(12738..13159,1..1334))
                     /gene="blaR1"
                     /locus_tag="FPO68_00215"
                     /pseudo=""
     CDS             complement(join(12738..13159,1..1334))
                     /gene="blaR1"
                     /locus_tag="FPO68_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /name="blaR1"
     gene            1441..2286
                     /gene="blaZ"
                     /locus_tag="FPO68_00220"
     ARG             1442..2283
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2812..3231)
                     /locus_tag="FPO68_00225"
     CDS             complement(2812..3231)
                     /locus_tag="FPO68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNZ34153.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            3355..4326
                     /locus_tag="FPO68_00230"
     CDS             3355..4326
                     /locus_tag="FPO68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNZ34154.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(4498..4590)
                     /locus_tag="FPO68_00235"
     CDS             complement(4498..4590)
                     /locus_tag="FPO68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNZ34155.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4747..5094)
                     /locus_tag="FPO68_00240"
     CDS             complement(4747..5094)
                     /locus_tag="FPO68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNZ34156.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(5113..5730)
                     /gene="cadD"
                     /locus_tag="FPO68_00245"
     ARG             complement(5117..5730)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6846..7040)
                     /locus_tag="FPO68_00250"
     CDS             complement(6846..7040)
                     /locus_tag="FPO68_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ34158.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            7150..7844
                     /locus_tag="FPO68_00255"
                     /pseudo=""
     CDS             7150..7844
                     /locus_tag="FPO68_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(8012..8161)
                     /locus_tag="FPO68_00260"
     CDS             complement(8012..8161)
                     /locus_tag="FPO68_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ34159.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(8708..9024)
                     /locus_tag="FPO68_00265"
                     /pseudo=""
     CDS             complement(8708..9024)
                     /locus_tag="FPO68_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(9017..10001)
                     /locus_tag="FPO68_00270"
                     /pseudo=""
     CDS             complement(9017..10001)
                     /locus_tag="FPO68_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            10427..11081
                     /locus_tag="FPO68_00275"
                     /pseudo=""
     CDS             10427..11081
                     /locus_tag="FPO68_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            complement(11529..12006)
                     /locus_tag="FPO68_00280"
                     /pseudo=""
     CDS             complement(11529..12006)
                     /locus_tag="FPO68_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(12003..12107)
                     /locus_tag="FPO68_00285"
     CDS             complement(12003..12107)
                     /locus_tag="FPO68_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNZ34160.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCERYLMTI"
                     /name="recombinase family protein"
     gene            complement(12369..12748)
                     /gene="blaI"
                     /locus_tag="FPO68_00290"
                     /pseudo=""
     CDS             complement(12369..12748)
                     /gene="blaI"
                     /locus_tag="FPO68_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /name="blaI"
     ARG             1442..2286
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(8505..8652)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(8711..8886)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(9018..10001)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.391"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10428..11169
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.063"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atacgttcga agtaccaatt aactgaattt tgcattgctg tatttaaatc ttgttcctta
       61 ttccaagact cgaaaagata atgtttatga ttccatgaca tacgtgaatt tttatcactt
      121 ataatatgac ggtcaagtcc aaacagtgct aaataaattt tgtaagttga atcaggagaa
      181 tatctttttc tactttcttt ttcattataa atatagtatt tgtctttctt catgctgtac
      241 ataacgaaag atccactatt tgaaccaaaa tttttacttt catctaaaat ttggtaatcg
      301 ctttgtaagg gctttttata attataatca gttaaggatt gccccattaa aaactggctt
      361 tgaattatca tgataaaaaa agtaaaaata caaataaaaa ttagaattaa ttttgactgc
      421 tttttcagat cggcttcttt tatattaatt aatctacttt tgagtaatga ttttttacca
      481 ttaaatgaat ggcttaatat atttttgttg gaaaatgggg tttttaatac ggagtccata
      541 atagattccg cataagtttt aaattcattt ttgttaatat tattaaggac aaatctatcg
      601 gcttctactt cattgtcatg aattatcttt ctttttacaa tatgtactaa tggattgtaa
      661 ctcataataa tagagaagat attaaagata attaaatgta aagtatctct atttttagca
      721 tgagcatatt catgcagtat tatatatttt agtcttttat caattacact tttaaaatat
      781 gaactaggaa tcaaaataat atatttccca taccaaaaag ttattggaga ttgaatagcc
      841 tctgcttttc gaatcacaat atttttttta tattgatggt tgaaaagtat cgtatctatt
      901 ttattttttt cattttcgtt tagataaagt gactgtttct ttaaatattt aagatataat
      961 aaggctttca aaaatttaaa acttaaaata ataactaaaa ctatccaaat aactgtgcag
     1021 atattatcaa ttgaatccca attaaactta tggatatctg ttgtgaactc ttgaataggt
     1081 ttggtggtat ttatgttatg gttcaaatcg tgtgacttac tttcaactgt gggcgcttga
     1141 ttattcacat tattaaattt tataaaagag aatttaattg ggataaaagg aattaatcca
     1201 gcaagcatag ttagatacca aactttataa tttaaactat aattaaaata gcgttttaat
     1261 atgtacctaa aaaatactaa caatagaaat ataaaacaaa agcttactac gctcattatt
     1321 agtaatttag ccattttgac accttctttc aaatatttat aataaacaat tgacatcaat
     1381 attacaattg taatattatt gatttataaa aattacaact gtaatatcgg agggtttatt
     1441 ttgaaaaagt taatattttt aattgtaatt gctttagttt taagtgcatg taattcaaac
     1501 agttcacatg ccaaagagtt aaatgattta gaaaaaaaat ataatgctca tattggtgtt
     1561 tatgctttaa atactaaaag tggtaaggaa gtaaaattta attcagataa gagatttgcc
     1621 tatgcttcga cttcaaaagc gataaatagt gctattttgt tagaacaagt accttataat
     1681 aagttaaata aaaaagtaca tattaacaaa gatgatatag ttgcttattc tcctatttta
     1741 gaaaaatatg taggaaaaga tatcacttta aaagaactta ttgaggcttc aatgacatat
     1801 agtgataata cagcaaacaa taaaattata aaagaaatcg gtggaatcaa aaaagttaaa
     1861 caacgtctaa aagaactagg agataaagta acaaatccag ttagatatga gatagaatta
     1921 aattactatt caccaaagag caaaaaagat acttcaacgc ctgctgcttt cggcaagact
     1981 ttaaataaac ttatcgcaaa tggaaaatta agcaaaaaaa ataaaaattt cttacttgat
     2041 ttaatgttta ataataaaaa cggagacact ttaattaaag atggtgttcc aaaagactat
     2101 aaggttgctg ataaaagtgg tcaagcaata acatatgctt ctagaaatga tgttgctttt
     2161 gtttatccta agggccaatc tgaacctatt gttttagtca tttttacgaa taaagacaat
     2221 aaaagtgata agccaaatga taagttgata agtgaaaccg ccaagagtgt aatgaaggaa
     2281 ttttaatatt ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt
     2341 gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc
     2401 gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat
     2461 aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta
     2521 tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa
     2581 agtgacaata gcattttaca agtatttttt attgttcgat tagggtaaac ctaaataata
     2641 cataaattat attacaatat gtttattata gaggctaaag tatcttagaa atttggatag
     2701 taggtttttc gggaataaaa gtatagttgg ctttaatata taattaagct aagctatcat
     2761 ttatatttac tcgattataa ttcgcacttc aactttttta taacgtaaaa attaaaacgt
     2821 agttttattt atctttagta atatcttttt aaacactaca gtttcttcat tagttaaatt
     2881 agcgaagtaa tgattattaa gttgataggc tttcttttct aatgttggcc ataagtcttt
     2941 tgctttattt gtactgttta ttagcacagt tcttctattt tcattatcag tttgacgtgt
     3001 tattagatcc tttttggaca tcttatctaa tattccagat attgttgaag gttctaaatt
     3061 taaagtatta ccaatcgctt taggtgtatt caaattacta ttaatcaagc aattcaaaac
     3121 accatattct gctggagtaa tattataggg acttaataaa cttttaaaat gtttaaaaac
     3181 tctattctga gaagtactca acaaataatt tatacaatta tctaattcca caactaaaac
     3241 ctctcttaaa tcattagtat tgactcgctt atcatatcat gataagtttt gtaggaaaat
     3301 agttttgcaa caaaaacata cttctacaaa acaattgtga aagaaggcta ataaatgttc
     3361 aatgcggtag tagtgagaaa aagtgcatca aatgttagtt ataaagtaga aaaagttgat
     3421 gaatctcatt tatcgaaggg tgatgtatta gtaaaagtgg tatactcttc aataaattat
     3481 aaggatatgc tagctttaca atataaaggc ggtgtaatta gagattatcc tatgattcct
     3541 ggtatagatt ttgcaggtat tgttgaatca agttcgaatg ataagtttaa agaaggcgat
     3601 aatgtattag ttactggtta tgatgttggc gtgacacata caggtggatt ttcagaatat
     3661 gcacaagtgc ctggagagtg gattgttcct attccccaag aaatgtctat taaagagtct
     3721 atggtatatg gcacagccgg ttttacggca gcattatcaa tttttgaact agaaaaatat
     3781 ggaatgaata tcaaatcaca acctgaaatt ttagtgtctg gggctacagg tggtgtgggc
     3841 agtgttgcta tacaaatttt acataaaatt ggatatgaaa atatcactgc tatcgtaaga
     3901 aaagaaaagc aaatagaaat cgcaaaaaaa ttaggtgcaa aacaagtaat aataattgaa
     3961 gatgataata aaccgcttaa aaaaggtatt tttgattatt tcttagatac agtggggagt
     4021 aatgtaacat tatacggttt aaaacgtcta aattatcaag gtgttgctac tgtatgtgga
     4081 aatgtagcag gaaattcact taacgctaat atattaccgt ttattcttag aggtattaaa
     4141 ttaattggca ttgattctgt atatgttgat cataatatcc gtgaagatat atggagtaag
     4201 ttagcaaatg aatggaatat aggaaattca cttctataca atgaaattgg atttgaagaa
     4261 ttctataaaa cgatagatca gcttttaaaa ggacaacatt taggaagaac tattttaaaa
     4321 gtataagtgt gagtattcat taatacattt actattaatt caatcatttt gcatttctct
     4381 actaaccagt gttataattt atataacgaa aatgattaac aattaatgca ccaatcccca
     4441 cactattgca gtagtgtggg gattttttcg atgctggctt ataaagtcgc ctattaatta
     4501 tttgttgcgt ttactaagcc aataagtata atacgcaaca gcacagccac tgatgactgg
     4561 tgctatgatg tgaacgaaaa tgattagcaa agtaatgcac ctcctctctt cgtcaattga
     4621 cgcctgagag ataggcgact ctattattat accatctgtg aatatacaag catatctact
     4681 tccgataagt cgtcttatgt tatataactt tgttttttat agtatcaaga caagaagaaa
     4741 ctcgtttcaa aaaccctcat gatttttaga gaaaaactct cttatttcat cgtctttaat
     4801 aaaataatat gccatctttc catctttata aaaatcaagt acctcatttt tatataaaag
     4861 ccttaaatga tgtgaagttg aagcaacact cattttcaat atcaaagaaa tatcacaaac
     4921 acacaactca tcttctttaa tcaaagataa tatgattttc aatttcttct catcacaaat
     4981 tttttctaaa atgttaagta atttcttaga tttatcatct tctaaaaaac ttaaagcatt
     5041 gttaacttta tcctcatgta cacagatcac atcacaagta ttttcataac tcatataaaa
     5101 tcacacccta aattaaaata taaatcctaa aattgtttga atagtgtcat tttcaataat
     5161 aataaataaa cctaaagcta tataaataat agccataatc caacgactaa atttctcaac
     5221 aatttctcca attcctggaa tattagctaa tttttgtgca gtaaacacca agaagaaaat
     5281 taaaattaaa aagacaaaca gagtaagtaa taaattagta acacttaatg tcacaaaata
     5341 cggaacaaat aaaccaatat tatcggcgcc acaacttgct atcgtaacaa ttgcaaccgt
     5401 accaactaat ttagacaatc ctttttcatt caattctttt ttagctctct tttctccttc
     5461 acaatcatca taaatagcca ctttaattcc aagataaatt ggtattaaac ccaataaacc
     5521 taatatccac ttttctggaa cataatttaa tacaaaagct aaaaacaaac taactaatat
     5581 taaaataata gaccctaaat attgaccaac ataaatatct ctatattctt ttctagtctt
     5641 tgctctagca aaaaatatta ataaaattac taataaatca actgctgtag caatataaag
     5701 aacagcagca gcaacaaccg tttgaatcat aatacacctc attcaaaaat atttttgaat
     5761 gtattataac ttatattttt aaataatcat aggtgcttta ttccaattgc tttattgacg
     5821 ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa tggtcggctt aatagctcac
     5881 gctatgccga cattcgtctg caagtttagt taagggttct tctcaacatc aataaatttt
     5941 ttcggcataa atgcgtgtct atgcgtagac atggagacac ttcagaatta tggagtgatt
     6001 acatgagttt aactattttt ctttattcag ttttaggaag tttattagca aattatatct
     6061 acgaaaaatc ttcaaaataa ataacttttt cggttctact ttgtacactt tatctacctg
     6121 gtagacctaa ttgttattgc tttacaggtc taaaattcgt cgttataatc tagttaaatc
     6181 ccgtaagggc gcacttatac gtcggataaa tcgacatgtg caaaaacgta atcgaccttc
     6241 gtcgattttt ttatttcaaa attaaagaaa cggcttgaga tattccgagc agttataaaa
     6301 attaatttgc gttgaatatt gctactacat tcttgaaaaa gtgatgtaga tttatagccc
     6361 ataacttttt tgaattacat cgacgatacg gcgtcgaaag aaaaataaat agaccgtatg
     6421 aaagaaaaag ctacctcact ttatctgggg tagcttttat tttttatgcc cttattttga
     6481 attttaagag ggcattttaa agatttaggg gtgattcata tagttttata tctaaaactt
     6541 atatatgctt ttaaaacgca aatatgagcc aaataaatat atgctatttt caaaaacaaa
     6601 aatttgagca aaaacaataa ctatttttag acactgccca tttacatgca aattaaaaat
     6661 tagcataaaa ttctttaaaa cttccccacg tttttctaaa ttcctgtagg gcgtttttat
     6721 caaaaatcaa gacaagaaga aactcgttca cactcgtttc aaaaaaacaa gaaatctaat
     6781 ctatacacct accgttttcc aaccccctca aagcatacaa attaaaaaca aaattattca
     6841 taatcttact ttcttttaac aaaaacataa aaaccataaa tagacaaatt aactatcaaa
     6901 ggcaataaac agtataaaaa aaccacgaat aaatcttcat caacatctaa aataaaaaag
     6961 aataaaacaa ttaacaaaat actaattaac aaacctaaaa gtggcaaact ttttatatat
     7021 ttttctaaat tttcattcat aaaatagact cctttcaaaa atatgtttat aaatatatta
     7081 aaataaatat tgattttata aagcgcttac ttatacaatt aaaatatctt attatcttgg
     7141 aggtatcaaa tgaaaattat aaaattattt tcaataattt ccttgttcct actggtattt
     7201 agcgcgaatt tttctaatgc ttacgcaaat gaagaacaaa aaagttcttt actagaaaat
     7261 caaaaagaaa aattgaaaag caaaaacatc gaagtatcac aaatgaccaa gaaagaaata
     7321 agttttctag aacaattgca aaataaaaat gaagatatgc aaaaattcga aaagcaaatg
     7381 aaaaaagaag gatttaaaaa aaataaagct agaagacaaa aattctaaat taaaatatct
     7441 agctacagaa gcaaatgata aaaatgcata tggctacata gctaataacg cttataaaaa
     7501 tgaaaaaaac aaaagaaatt gtagtttctg aaacaatata tgataactac aacaaaaaaa
     7561 tcacaaaatt cattgccgaa aaaagaagta tgactgaaaa caaagaggga aaaatactta
     7621 ttaacaaaaa ttacgttgaa tataaggctg aaaaagttga taaaaacaga ccggtaacat
     7681 atgcaggatt caaatggaac ggcaaagcat tcgcttgtag tatggctgga ctttatgcat
     7741 gtgctcacta ctgtggagta tgggcaatcg taaatccaat agccggcggt gtatgtgaag
     7801 cagtttgcgg aactgcattt gcagctggat gctctgtagg ttgattttat tccaattgtt
     7861 ttattgacgt tgagcctcgg aacccttaac aatcccaaaa cttgtcgaat ggtcggctta
     7921 atagctcacg ctatgccgac attcgtctgc aagtttagtt aagggttctt ctcaacatca
     7981 ataaattttt tcggcataaa tacgatgttc cttatttatc ctcataaaaa acaatagctg
     8041 aaatatataa ccaattactg tgtgtttgaa tttctaatac atgaatatca ttctttaaaa
     8101 acctattaac ttgtttatct aattgacgat gagtaaaagt agcgaaagtt ttaatcttca
     8161 tgatatctct ccttttataa aaatagcaat ccttattaca atattaaaca cttttatttt
     8221 ggattttaag aggcattttg aagacttaga ggcaaatcat ataggttttg tacttaaaaa
     8281 tctatacagt ctcttaaaac gcaaatatga gccaaataaa tatatgctaa tttcacaaac
     8341 aaaaatttca gcaaaatcag tgactaattt tttagatact gcccatttac atgcaaattt
     8401 aaaaattagc ataaaatact ttaaaacttc cccatgtttt tctaaattcc tgcagggctt
     8461 ttcttatcaa aaacttagaa attttaattt aactctaaaa tctccgtaag agcataaggg
     8521 aaatttgcta tcattcaaaa atgatagatc tggcaaggcc agacattcca agggttttaa
     8581 gttattttaa agtgatatca tacaaagctg tagtgtcaca cttttgtgac actacataca
     8641 gcatttgtca caaaaataag acagcacttt tttacagaaa aacaataaaa aaagacatca
     8701 gttatcacta atgtcttaaa taataatttt gcttttcgaa attccaaatt gcggtaattg
     8761 aatgtgcttt ttttcttcgt ctgtcgtttt atcttcatca cttgtattaa tcaaattacc
     8821 atcttcggca tcgtctaagt ttaaaatttt atgcttttgt ttaagcaatc caccataact
     8881 taacatacgt tttcgataca aacctttttc taaatcactt acaatttctt gatttttttc
     8941 gtcgtcatca gttaaaaagt cagatgattt aacggaatat tttgatgtct cttcgagtgt
     9001 tataaaatca tttgaattat tgattaaaga tattatattg tttcatgttt ttacggcgca
     9061 tataaagggc atgtgtttct tcaagttcag ttagaattgt ttgcattaaa taagcttgca
     9121 ttgacttgat tgtttccttt tggcctttaa ctcccctatt atgtatagct tttttaagtc
     9181 tttttaaaga agttaatatt tcatcctcaa tgtcctctaa catgtaatgc atatcttcat
     9241 gccttgaatt aaatgatttt tttgctttaa gaataacagc ctttatgatt tctaaatctc
     9301 tataatcata ttttaataaa taattagtta gttgttctgg gaattcttgc aatagcattt
     9361 ctttatcatc aaaattatgt gtaaattgat ttgaatgatt tgaatgatta ctaatttcat
     9421 tttttgtact ttatatcatt caagtcattc atatcattat tctcagtatc attaaagtca
     9481 atgtcattaa agtcagtatc attagtgttc gattttcgaa cttctggacg ttcgattttc
     9541 gaacttctgg acgttcgatt ttcgaacttc tggacgttcg attttcgaac ttccttctcg
     9601 ccgtaaggtt caacatcgtt ttcttctttt tctattttgt aaatatcacg ttctgtgaca
     9661 ataggtttca ttaaatatag aatatttggt ttatttaaac cacgtcgttt ttgaatgagt
     9721 agaccatcgt tttctaaccc tttttttatt tttgttaact tttctttttt acaatttaag
     9781 atttccatta gcttttcatt tgaataaaca aagtaaatgt taccatcttc atcaacccag
     9841 ctatttttaa tagataaatt taagcgatca cgtaagatag cataggctat tttcatatca
     9901 ttagttagat ttttataatt ttcactggta aagaatactt tagggatttg ataaaatctt
     9961 tctttgtaat tttcttgcac tgtaaaatat tgattttgca tgacaaaaaa cctctttcca
    10021 tattttagtt tggtaagagg taataaaacg tttttattta gctttacatt tatttaatga
    10081 tttaggtgct tgcactaaga tattattcat tatataatac aagtatcgaa aattatataa
    10141 ataaacagtt tctaaacgtt ttattaccaa accccgacta ttaccagtag ttggggtttt
    10201 tgttatatta agtttattat agcactttaa ataaacttat aaaagggaaa atatagcttt
    10261 atcaagggtt tataaaccct tgataaatat cataaacctt tcataaaccc ttgataaaaa
    10321 ggttgatgga tgtttatttt tatttattaa tcactttatt attaagcatt ataagcttat
    10381 aatattttat caagggttta taaacatttt ataaagggtt gatgatatga aaagtgtgaa
    10441 aaagttgagt gaagaattag gggttagtaa gcaaacgatt tttaataata ttaagcgatt
    10501 gaatatagag acaataaaac aggaaaacac ttcgtttatt aaagaagata ccgatataga
    10561 aaaaatcatt caaagagtaa atgaaaataa aaagaaatat ggttttgaaa gtactactga
    10621 agacaaacaa aaaaaagggt cggataatat taattttgaa tcgaaaagcg atgctcaaat
    10681 agtagaaatc cttaaaaatc agattgatac tttaaataat caaattgaaa aacaagaaag
    10741 tcgtcatgaa acaacaattg aattttacag aaaagagttg caagaaagat caaaattgct
    10801 tgaaaatcag caagttctgg cattagagag taataaaaag attcaaaaat tagaaaatca
    10861 attagaaaaa gaaaaacacc ttaattatcc ctttgataca tctactaaca ctagacaaaa
    10921 cgttgatgca caagaaaaac atatactact aatcctgtaa atattaacag agatcaggaa
    10981 gaaactaaga aacagagatt caatataagg atatatctgg aagtcaaagc gacgaaagta
    11041 cacaaggtga agaagcaaaa aaaagaagac gttccagcta atctaaatga caatgatagt
    11101 gaatagaaga aaagagtgaa gaaaacggaa acctaaaaag ggttttggag tcgtttgttt
    11161 ggtagctaaa ataattgatt aggcactaaa aagttataaa attttagtgt ctttattata
    11221 gtgagaactt ctggtaatca tcgatatgta aactaaagta tttcaaataa ttaactgtat
    11281 taatactctc atcatgattg cgttataaac gttcattagt gaaacaggta ttatattaaa
    11341 aaagcgtacg aatttgtacg cttttttctt gtgttattat taaaatctca taacaggaaa
    11401 atattatgag attttagacg ctgtcagaac gtctctttta catcgagtga cattgaatca
    11461 aagtctttgt atgttaatga tatataaatt atttagtaga ttcattatag taagctagat
    11521 tagttgctct acttgttttt attctctttt gcctctaatt tattgagata acgataaata
    11581 gttgtacgtg aaacttgcca ctgctctgca attgttttaa ttggcgtacc attatcataa
    11641 agtgtcttaa gtaagtttaa atctgttttt atttaatttt tcaggtctac ctccatatcg
    11701 gcctctagca cgtgctgcaa ttcgaccggc tgatgatcgc tctaaaatta aattacgttc
    11761 aaactctgca aatgctgcga ataaatgaaa taataattgt cctgtagaac ttgatttatc
    11821 catcgttata tttttcttct aaactatgaa aactcacacc tcgttcgtta agttcattaa
    11881 ctaacgtgat taatcctcca tgttccgtcc tagtctatct aatctccaaa caacaatcgt
    11941 atctcctgac ctcgcaaatt caattgcttt atctaaacca ggtcgcttac tttttgaacc
    12001 actcatatgg tcattaaata tctttcacaa ccatatgtat ttaatcgatc ctcctgtaaa
    12061 ttcaaatttt gtaatccagt tgaaactctt gcataaccta ttttcaaaat acagacctcc
    12121 tatatacctt tagtgtacca taacttgatt acgaggtctt ttgtgaacat agattttttg
    12181 ttgggttttt gaacacataa ttctatgtaa tacttactta tttagacaaa aaacaagagt
    12241 gtacagaaaa tggttatttt ctggacactc tcatctttaa taatatattt taaatgtcat
    12301 taacaaaact aatttaataa gagtcaagta tggtttacat atgtattatg tttgaaacat
    12361 acctctaatt actttttact aatatcattt aaaatgtctc gcaattcttc aatttctttg
    12421 ttatttaatt ttcatttttt tcgcaaaatt cagcactaaa cttttcatat cccctccata
    12481 cagtttatta agaaaggttt tagcagtttt cattttaata tcgtcttctt taatatttga
    12541 tgagtaaaaa taaatatttt ctgatttgta tcgttttata atctcttttt tatatagtct
    12601 tgtgattaat gttctaatcg ttttatctaa cttctttata tttttgaatt tcaactacaa
    12661 tttcattagc tgatactgat tttttatccc atattatatt cataacatcc cattcagcca
    12721 tagatatttc aacttgctta ttggtcattt aaaacaccca ttccttttaa tattttttca
    12781 cttattaatt cagcattttt cccagacggg tttccatctg ataaatgtgt agcaaaataa
    12841 tacttatcat gatttgtaat tacgtaacct acaaaccacc catttattac ttcccgttta
    12901 ctatacctgt acctgttttc ccatacagtt catacttttc atttttctta atcaataatg
    12961 aagaagataa ttgattcttt gcttttttac taaaatggtt attttgttcc atcatatttt
    13021 aaaaactatt acttgttcaa gattagatat tttcaaacta tcttccatcc aatagctttt
    13081 ataacttccc aaattttcat taccataatt taattgcttg agttgagccg cagtatagtt
    13141 ctttggtatt tgattacta
//
