LOCUS       CP049516               13163 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt215 plasmid pER05718.3A.3, complete
            sequence.
ACCESSION   CP049516
VERSION     CP049516.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331032
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 479.743284893992x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,769
            CDSs (with protein)               :: 2,769
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 40 of 70
            Pseudo Genes (incomplete)         :: 22 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt215"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER05718.3A.3"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            join(13163,1..1757)
                     /gene="blaR1"
                     /locus_tag="FPO78_14535"
     ARG             4..1754
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="99.83"
     gene            1747..2127
                     /gene="blaI"
                     /locus_tag="FPO78_14540"
     ARG             1748..2124
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            2391..2969
                     /locus_tag="FPO78_14545"
     CDS             2391..2969
                     /locus_tag="FPO78_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNZ14937.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(3330..4073)
                     /locus_tag="FPO78_14550"
     CDS             complement(3330..4073)
                     /locus_tag="FPO78_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QNZ14938.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            4499..5482
                     /locus_tag="FPO78_14555"
     CDS             4499..5482
                     /locus_tag="FPO78_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QNZ14939.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            5475..5791
                     /locus_tag="FPO78_14560"
                     /pseudo=""
     CDS             5475..5791
                     /locus_tag="FPO78_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            6338..6487
                     /locus_tag="FPO78_14565"
     CDS             6338..6487
                     /locus_tag="FPO78_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ14940.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(6655..7347)
                     /locus_tag="FPO78_14570"
     CDS             complement(6655..7347)
                     /locus_tag="FPO78_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ14941.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            7457..7651
                     /locus_tag="FPO78_14575"
     CDS             7457..7651
                     /locus_tag="FPO78_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNZ14942.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            8767..9384
                     /gene="cadD"
                     /locus_tag="FPO78_14580"
     ARG             8768..9381
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9403..9750
                     /locus_tag="FPO78_14585"
     CDS             9403..9750
                     /locus_tag="FPO78_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNZ14944.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            9907..9999
                     /locus_tag="FPO78_14590"
     CDS             9907..9999
                     /locus_tag="FPO78_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNZ14945.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10171..11142)
                     /locus_tag="FPO78_14595"
     CDS             complement(10171..11142)
                     /locus_tag="FPO78_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNZ14946.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            11266..11685
                     /locus_tag="FPO78_14600"
     CDS             11266..11685
                     /locus_tag="FPO78_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNZ14947.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(12211..13056)
                     /gene="blaZ"
                     /locus_tag="FPO78_14605"
     ARG             complement(12215..13056)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(12212..13056)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          5848..5995
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(3331..4073)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4500..5482
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5614..5783
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 tggctaaatt actaataatg agcgtagtaa gcttttgttt tatatttcta ttgttagtat
       61 tttttaggta catattaaaa cgctatttta attatagttt aaattataaa gtttggtatc
      121 taactatgct tgctggatta attcctttta tcccaattaa attctctttt ataaaattta
      181 ataatgtgaa taatcaagcg cccacagttg aaagtaagtc acacgatttg aaccataaca
      241 taaataccac caaacctatt caagagttca caacagatat ccataagttt aattgggatt
      301 caattgataa tatctgcaca gttatttgga tagttttagt tattatttta agttttaaat
      361 ttttgaaagc cttattatat cttaaatatt taaagaaaca gtcactttat ctaaacgaaa
      421 atgaaaaaaa taaaatagat acgatacttt tcaaccatca atataaaaaa aatattgtga
      481 ttcgaaaagc agaggctatt caatctccaa taactttttg gtatgggaaa tatattattt
      541 tgattcctag ttcatatttt aaaagtgtaa ttgataaaag actaaaatat ataatactgc
      601 atgaatatgc tcatgctaaa aatagagata ctttacattt aattatcttt aatatcttct
      661 ctattattat gagttacaat ccattagtac atattgtaaa aagaaagata attcatgaca
      721 atgaagtaga agccgataga tttgtcctta ataatattaa caaaaatgaa tttaaaactt
      781 atgcggaatc tattatggac tccgtattaa aaaccccatt ttccaacaaa aatatattaa
      841 gccattcatt taatggtaaa aaatcattac tcaaaagtag attaattaat ataaaagaag
      901 ccgatctgaa aaagcagtca aaattaattc taatttttat ttgtattttt acttttttta
      961 tcatgataat tcaaagccag tttttaatgg ggcaatcctt aactgattat aattataaaa
     1021 agcccttaca aagcgattac caaattttag atgaaagtaa aaattttggt tcaaatagtg
     1081 gatctttcgt tatgtacagc atgaagaaag acaaatacta tatttataat gaaaaagaaa
     1141 gtagaaaaag atattctcct gattcaactt acaaaattta tttagcactg tttggacttg
     1201 accgtcatat tataagtgat aaaaattcac gtatgtcatg gaatcataaa cattatcttt
     1261 tcgagtcttg gaataaggaa caagatttaa atacagcaat gcaaaattca gttaattggt
     1321 acttcgaacg tattagtaat caaataccaa agaactatac tgcggctcaa ctcaagcaat
     1381 taaattatgg taatgaaaat ttgggaagtt ataaaagcta ttggatggaa gatagtttga
     1441 aaatatctaa tcttgaacaa gtaatagttt ttaaaaatat gatggaacaa aataaccatt
     1501 ttagtaaaaa agcaaagaat caattatctt cttcattatt gattaagaaa aatgaaaagt
     1561 atgaactgta tgggaaaaca ggtacaggta tagtaaacgg gaagtataat aatgggtggt
     1621 ttgtaggtta cgtaattaca aatcatgata agtattattt tgctacacat ttatcagatg
     1681 gaaacccgtc tgggaaaaat gctgaattaa taagtgaaaa aatattaaaa ggaatgggtg
     1741 ttttaaatga ccaataagca agttgaaata tctatggctg aatgggatgt tatgaatata
     1801 atatgggata aaaaatcagt atcagctaat gaaattgtag ttgaaattca aaaatataaa
     1861 gaagttagtg ataaaacgat tagaacatta atcacaagac tatataaaaa agagattata
     1921 aaacgataca aatcagaaaa tatttatttt tactcatcaa atattaaaga agacgatatt
     1981 aaaatgaaaa ctgctaaaac ctttcttaat aaactgtatg gaggggatat gaaaagttta
     2041 gtgctgaatt ttgcgaaaaa tgaagaatta aataacaaag aaattgaaga attgcgagac
     2101 attttaaatg atattagtaa aaagtaatta gaggtatgtt tcaaacataa tacatatgta
     2161 aaccatactt gactcttatt aaattagttt tgttaatgac atttaaaata tattattaaa
     2221 gatgagagtg tccagaaaat aaccattttc tgtacactct tgttttttgt ctaaataagt
     2281 aagtattaca tagaattatg tgttcaaaaa cccaacaaaa aatctatgtt cacaaaagac
     2341 ctcgtaatca agttatggta cactaaaggt gtatatagga ggtctgtatt ttgaaaatag
     2401 gttatgcaag agtttcaact ggattacaaa atttgaattt acaggaggat cgattaaata
     2461 catatggttg tgaaaagata tttaatgacc atatgagtgg ttcaaaaagt aagcgacctg
     2521 gtttagataa agcaattgaa tttgcgaggt caggagatac gattgttgtt tggagattag
     2581 atagactagg acggaacatg gaggatttaa tcacgttagt taatgaactt aacgaacgag
     2641 gtgtgagttt tcatagttta gaagaaaata taacgatgga taaatcaagt tctacaggac
     2701 aattattatt tcatttattc gcagcatttg cagagtttga acgtaattta attttagagc
     2761 gatcatcagc cggtcgaatt gcagcacgtg ctagaggccg atatggaggt agacctgaaa
     2821 aattaaataa acaagattta aacttactta agacacttta tgataatggt acgccaatta
     2881 aaacaattgc agagcagtgg caagtttcac gtacaactat ttatcgttat ctcaataaat
     2941 tagaggcaaa agagaataaa aacaagtaga gcaactaatc tagcttacta taatgaatct
     3001 actaaataat tttatatatc attaacatac aaagactttg attcaatgtc actcgatgta
     3061 aaagagacgt tctgacagcg tctaaaatct cataatattt tcctgttatg agattttaat
     3121 aataacacaa gaaaaaagcg tacaaattcg tacgcttttt taatataata cctgtttcac
     3181 taatgaacgt ttataacgca atcatgatga gagtattaat acagttaatt atttgaaata
     3241 ctttagttta cataatcgat gattaccaga agttctcact atataaaaga cactaaaatt
     3301 ttataacttt tagtgcctaa tcaattattt tagctaccaa acaaacgact ccaaaacccc
     3361 tttttaggtt ccgtttcttc actcttttct tctatatcac tatcattgtc atttagatta
     3421 gctggaacgt cttctttttt tgcttcttca ccttgtgtac tttcgtcgct ttgacttcca
     3481 gatatatcct tatattgaat ctctgtttct ttagtttctt cctgatctct gttaatattt
     3541 acaggattag tagtatatgt tttttcttgt gcatcaacgt tttgtctagt gttagtagat
     3601 gtatcaaagg gataattaag gtgtttttct ttttctaatt gattttctaa tttttgaatc
     3661 tttttattac tctctaatgc cagaacttgc tgattttcaa gcaattttga tctttcttgc
     3721 aactcttttc tgtaaaattc aattgttgtt tcatgacgac tttcttgttt ttcaatttga
     3781 ttatttaaag tatcaatctg atttttaagg atttctacta tttgagcatc gcttttcgat
     3841 tcaaaattaa tattatccga cccttttttt tgtttgtctt cagtagtact ttcaaaacca
     3901 tatttctttt tattttcatt tactctttga atgatttttt ctatatcggt atcttcttta
     3961 ataaacgaag tgttttcctg ttttattgtc tctatattca atcgcttaat attattaaaa
     4021 atcgtttgct tactaacccc taattcttca ctcaactttt tcacactttt catatcatca
     4081 accctttata aaatgtttat aaacccttga taaaatatta taagcttata atgcttaata
     4141 ataaagtgat taataaataa aaataaacat ccatcaacct ttttatcaag ggtttatgaa
     4201 aggtttatga tatttatcaa gggtttataa acccttgata aagctatatt ttccctttta
     4261 taagtttatt taaagtgcta taataaactt aatataacaa aaaccccaac tactggtaat
     4321 agtcggggtt tggtaataaa acgtttagaa actgtttatt tatataattt tcgatacttg
     4381 tattatataa tgaataatat cttagtgcaa gcacctaaat cattaaataa atgtaaagct
     4441 aaataaaaac gttttattac ctcttaccaa actaaaatat ggaaagaggt tttttgtcat
     4501 gcaaaatcaa tattttacag tgcaagaaaa ttacaaagaa agattttatc aaatccctaa
     4561 agtattcttt accagtgaaa attataaaaa tctaactaat gatatgaaaa tagcctatgc
     4621 tatcttacgt gatcgcttaa atttatctat taaaaatagc tgggttgatg aagatggtaa
     4681 catttacttt gtttattcaa atgaaaagct aatggaaatc ttaaattgta aaaaagaaaa
     4741 gttaacaaaa ataaaaaaag ggttagaaaa cgatggtcta ctcattcaaa aacgacgtgg
     4801 tttaaataaa ccaaatattc tatatttaat gaaacctatt gtcacagaac gtgatattta
     4861 caaaatagaa aaagaagaaa acgatgttga accttacggc gagaaggaag ttcgaaaatc
     4921 gaacgtccag aagttcgaaa atcgaacgtc cagaagttcg aaaatcgaac gtccagaagt
     4981 tcgaaaatcg aacactaatg atactgactt taatgacatt gactttaatg atactgagaa
     5041 taatgatatg aatgacttga atgatataaa gtacaaaaat gaaattagta atcattcaaa
     5101 tcattcaaat caatttacac ataattttga tgataaagaa atgctattgc aagaattccc
     5161 agaacaacta actaattatt tattaaaata tgattataga gatttagaaa tcataaaggc
     5221 tgttattctt aaagcaaaaa aatcatttaa ttcaaggcat gaagatatgc attacatgtt
     5281 agaggacatt gaggatgaaa tattaacttc tttaaaaaga cttaaaaaag ctatacatga
     5341 taggggagtt aaaggccaaa aggaaacaat caagtcaatg caagcttatt taatgcaaac
     5401 aattctaact gaacttgaag aaacacatgc cctttatatg cgccgtaaaa acatgaaaca
     5461 atataatatc tttaatcaat aattcaaatg attttataac actcgaagag acatcaaaat
     5521 attccgttaa atcatctgac tttttaactg atgacgacga aaaaaatcaa gaaattgtaa
     5581 gtgatttaga aaaaggtttg tatcgaaaac gtatgttaag ttatggtgga ttgcttaaac
     5641 aaaagcataa aattttaaac ttagacgatg ccgaagatgg taatttgatt aatacaagtg
     5701 atgaagataa aacgacagac gaagaaaaaa agcacattca attaccgcaa tttggaattt
     5761 cgaaaagcaa aattattatt taagacatta gtgataactg atgtcttttt ttattgtttt
     5821 tctgtaaaaa agtgctgtct tatttttgtg acaaatgctg tatgtagtgt cacaaaagtg
     5881 tgacactaca gctttgtatg atatcacttt aaaataactt aaaacccttg gaatgtctgg
     5941 ccttgccaga tctatcattt ttgaatgata gcaaatttcc cttatgctct tacggagatt
     6001 ttagagttaa attaaaattt ctaagttttt gataagaaaa gccctgcagg aatttagaaa
     6061 aacatgggga agttttaaag tattttatgc taatttttaa atttgcatgt aaatgggcag
     6121 tatctaaaaa attagtcact gattttgctg aaatttttgt ttgtgaaatt agcatatatt
     6181 tatttggctc atatttgcgt tttaagagac tgtatagatt tttaagtaca aaacctatat
     6241 gatttgcctc taagtcttca aaatgcctct taaaatccaa aataaaagtg tttaatattg
     6301 taataaggat tgctattttt ataaaaggag agatatcatg aagattaaaa ctttcgctac
     6361 ttttactcat cgtcaattag ataaacaagt taataggttt ttaaagaatg atattcatgt
     6421 attagaaatt caaacacaca gtaattggtt atatatttca gctattgttt tttatgagga
     6481 taaataagga acatcgtatt tatgccgaaa aaatttattg atgttgagaa gaacccttaa
     6541 ctaaacttgc agacgaatgt cggcatagcg tgagctatta agccgaccat tcgacaagtt
     6601 ttgggattgt taagggttcc gaggctcaac gtcaataaaa caattggaat aaaatcaacc
     6661 tacagagcat ccagctgcaa atgcagttcc gcaaactgct tcacatacac cgccggctat
     6721 tggatttacg attgcccata ctccacagta gtgagcacat gcataaagtc cagccatact
     6781 acaagcgaat gctttgccgt tccatttgaa tcctgcatat gttaccggtc tgtttttatc
     6841 aactttttca gccttatatt caacgtaatt tttgttaata agtatttttc cctctttgtt
     6901 ttcagtcata cttctttttt cggcaatgaa ttttgtgatt tttttgttgt agttatcata
     6961 tattgtttca gaaactacaa tttcttttgt tttttcattt ttataagcgt tattagctat
     7021 gtagccatat gcatttttat catttgcttc tgtagctaga tattttaatt tagaattttt
     7081 gtcttctagc tttatttttt taaatccttc ttttttcatt tgcttttcga atttttgcat
     7141 atcttcattt ttattttgca attgttctag aaaacttatt tctttcttgg tcatttgtga
     7201 tacttcgatg tttttgcttt tcaatttttc tttttgattt tctagtaaag aacttttttg
     7261 ttcttcattt gcgtaagcat tagaaaaatt cgcgctaaat accagtagga acaaggaaat
     7321 tattgaaaat aattttataa ttttcatttg atacctccaa gataataaga tattttaatt
     7381 gtataagtaa gcgctttata aaatcaatat ttattttaat atatttataa acatattttt
     7441 gaaaggagtc tattttatga atgaaaattt agaaaaatat ataaaaagtt tgccactttt
     7501 aggtttgtta attagtattt tgttaattgt tttattcttt tttattttag atgttgatga
     7561 agatttattc gtggtttttt tatactgttt attgcctttg atagttaatt tgtctattta
     7621 tggtttttat gtttttgtta aaagaaagta agattatgaa taattttgtt tttaatttgt
     7681 atgctttgag ggggttggaa aacggtaggt gtatagatta gatttcttgt ttttttgaaa
     7741 cgagtgtgaa cgagtttctt cttgtcttga tttttgataa aaacgcccta caggaattta
     7801 gaaaaacgtg gggaagtttt aaagaatttt atgctaattt ttaatttgca tgtaaatggg
     7861 cagtgtctaa aaatagttat tgtttttgct caaatttttg tttttgaaaa tagcatatat
     7921 ttatttggct catatttgcg ttttaaaagc atatataagt tttagatata aaactatatg
     7981 aatcacccct aaatctttaa aatgccctct taaaattcaa aataagggca taaaaaataa
     8041 aagctacccc agataaagtg aggtagcttt ttctttcata cggtctattt atttttcttt
     8101 cgacgccgta tcgtcgatgt aattcaaaaa agttatgggc tataaatcta catcactttt
     8161 tcaagaatgt agtagcaata ttcaacgcaa attaattttt ataactgctc ggaatatctc
     8221 aagccgtttc tttaattttg aaataaaaaa atcgacgaag gtcgattacg tttttgcaca
     8281 tgtcgattta tccgacgtat aagtgcgccc ttacgggatt taactagatt ataacgacga
     8341 attttagacc tgtaaagcaa taacaattag gtctaccagg tagataaagt gtacaaagta
     8401 gaaccgaaaa agttatttat tttgaagatt tttcgtagat ataatttgct aataaacttc
     8461 ctaaaactga ataaagaaaa atagttaaac tcatgtaatc actccataat tctgaagtgt
     8521 ctccatgtct acgcatagac acgcatttat gccgaaaaaa tttattgatg ttgagaagaa
     8581 cccttaacta aacttgcaga cgaatgtcgg catagcgtga gctattaagc cgaccattcg
     8641 acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa
     8701 gcacctatga ttatttaaaa atataagtta taatacattc aaaaatattt ttgaatgagg
     8761 tgtattatga ttcaaacggt tgttgctgct gctgttcttt atattgctac agcagttgat
     8821 ttattagtaa ttttattaat attttttgct agagcaaaga ctagaaaaga atatagagat
     8881 atttatgttg gtcaatattt agggtctatt attttaatat tagttagttt gtttttagct
     8941 tttgtattaa attatgttcc agaaaagtgg atattaggtt tattgggttt aataccaatt
     9001 tatcttggaa ttaaagtggc tatttatgat gattgtgaag gagaaaagag agctaaaaaa
     9061 gaattgaatg aaaaaggatt gtctaaatta gttggtacgg ttgcaattgt tacgatagca
     9121 agttgtggcg ccgataatat tggtttattt gttccgtatt ttgtgacatt aagtgttact
     9181 aatttattac ttactctgtt tgtcttttta attttaattt tcttcttggt gtttactgca
     9241 caaaaattag ctaatattcc aggaattgga gaaattgttg agaaatttag tcgttggatt
     9301 atggctatta tttatatagc tttaggttta tttattatta ttgaaaatga cactattcaa
     9361 acaattttag gatttatatt ttaatttagg gtgtgatttt atatgagtta tgaaaatact
     9421 tgtgatgtga tctgtgtaca tgaggataaa gttaacaatg ctttaagttt tttagaagat
     9481 gataaatcta agaaattact taacatttta gaaaaaattt gtgatgagaa gaaattgaaa
     9541 atcatattat ctttgattaa agaagatgag ttgtgtgttt gtgatatttc tttgatattg
     9601 aaaatgagtg ttgcttcaac ttcacatcat ttaaggcttt tatataaaaa tgaggtactt
     9661 gatttttata aagatggaaa gatggcatat tattttatta aagacgatga aataagagag
     9721 tttttctcta aaaatcatga gggtttttga aacgagtttc ttcttgtctt gatactataa
     9781 aaaacaaagt tatataacat aagacgactt atcggaagta gatatgcttg tatattcaca
     9841 gatggtataa taatagagtc gcctatctct caggcgtcaa ttgacgaaga gaggaggtgc
     9901 attactttgc taatcatttt cgttcacatc atagcaccag tcatcagtgg ctgtgctgtt
     9961 gcgtattata cttattggct tagtaaacgc aacaaataat taataggcga ctttataagc
    10021 cagcatcgaa aaaatcccca cactactgca atagtgtggg gattggtgca ttaattgtta
    10081 atcattttcg ttatataaat tataacactg gttagtagag aaatgcaaaa tgattgaatt
    10141 aatagtaaat gtattaatga atactcacac ttatactttt aaaatagttc ttcctaaatg
    10201 ttgtcctttt aaaagctgat ctatcgtttt atagaattct tcaaatccaa tttcattgta
    10261 tagaagtgaa tttcctatat tccattcatt tgctaactta ctccatatat cttcacggat
    10321 attatgatca acatatacag aatcaatgcc aattaattta atacctctaa gaataaacgg
    10381 taatatatta gcgttaagtg aatttcctgc tacatttcca catacagtag caacaccttg
    10441 ataatttaga cgttttaaac cgtataatgt tacattactc cccactgtat ctaagaaata
    10501 atcaaaaata ccttttttaa gcggtttatt atcatcttca attattatta cttgttttgc
    10561 acctaatttt tttgcgattt ctatttgctt ttcttttctt acgatagcag tgatattttc
    10621 atatccaatt ttatgtaaaa tttgtatagc aacactgccc acaccacctg tagccccaga
    10681 cactaaaatt tcaggttgtg atttgatatt cattccatat ttttctagtt caaaaattga
    10741 taatgctgcc gtaaaaccgg ctgtgccata taccatagac tctttaatag acatttcttg
    10801 gggaatagga acaatccact ctccaggcac ttgtgcatat tctgaaaatc cacctgtatg
    10861 tgtcacgcca acatcataac cagtaactaa tacattatcg ccttctttaa acttatcatt
    10921 cgaacttgat tcaacaatac ctgcaaaatc tataccagga atcataggat aatctctaat
    10981 tacaccgcct ttatattgta aagctagcat atccttataa tttattgaag agtataccac
    11041 ttttactaat acatcaccct tcgataaatg agattcatca actttttcta ctttataact
    11101 aacatttgat gcactttttc tcactactac cgcattgaac atttattagc cttctttcac
    11161 aattgttttg tagaagtatg tttttgttgc aaaactattt tcctacaaaa cttatcatga
    11221 tatgataagc gagtcaatac taatgattta agagaggttt tagttgtgga attagataat
    11281 tgtataaatt atttgttgag tacttctcag aatagagttt ttaaacattt taaaagttta
    11341 ttaagtccct ataatattac tccagcagaa tatggtgttt tgaattgctt gattaatagt
    11401 aatttgaata cacctaaagc gattggtaat actttaaatt tagaaccttc aacaatatct
    11461 ggaatattag ataagatgtc caaaaaggat ctaataacac gtcaaactga taatgaaaat
    11521 agaagaactg tgctaataaa cagtacaaat aaagcaaaag acttatggcc aacattagaa
    11581 aagaaagcct atcaacttaa taatcattac ttcgctaatt taactaatga agaaactgta
    11641 gtgtttaaaa agatattact aaagataaat aaaactacgt tttaattttt acgttataaa
    11701 aaagttgaag tgcgaattat aatcgagtaa atataaatga tagcttagct taattatata
    11761 ttaaagccaa ctatactttt attcccgaaa aacctactat ccaaatttct aagatacttt
    11821 agcctctata ataaacatat tgtaatataa tttatgtatt atttaggttt accctaatcg
    11881 aacaataaaa aatacttgta aaatgctatt gtcactttgc ttgatatatg agaattattt
    11941 aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct ttttcgattg
    12001 atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat atacaatatt
    12061 tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg gaaaatcagt
    12121 ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa actataagat
    12181 gttatcagta tttattatgc atttagaata ttaaaattcc ttcattacac tcttggcggt
    12241 ttcacttatc aacttatcat ttggcttatc acttttattg tctttattcg taaaaatgac
    12301 taaaacaata ggttcagatt ggcccttagg ataaacaaaa gcaacatcat ttctagaagc
    12361 atatgttatt gcttgaccac ttttatcagc aaccttatag tcttttggaa caccatcttt
    12421 aattaaagtg tctccgtttt tattattaaa cattaaatca agtaagaaat ttttattttt
    12481 tttgcttaat tttccatttg cgataagttt atttaaagtc ttgccgaaag cagcaggcgt
    12541 tgaagtatct tttttgctct ttggtgaata gtaatttaat tctatctcat atctaactgg
    12601 atttgttact ttatctccta gttcttttag acgttgttta acttttttga ttccaccgat
    12661 ttcttttata attttattgt ttgctgtatt atcactatat gtcattgaag cctcaataag
    12721 ttcttttaaa gtgatatctt ttcctacata tttttctaaa ataggagaat aagcaactat
    12781 atcatctttg ttaatatgta cttttttatt taacttatta taaggtactt gttctaacaa
    12841 aatagcacta tttatcgctt ttgaagtcga agcataggca aatctcttat ctgaattaaa
    12901 ttttacttcc ttaccacttt tagtatttaa agcataaaca ccaatatgag cattatattt
    12961 tttttctaaa tcatttaact ctttggcatg tgaactgttt gaattacatg cacttaaaac
    13021 taaagcaatt acaattaaaa atattaactt tttcaaaata aaccctccga tattacagtt
    13081 gtaattttta taaatcaata atattacaat tgtaatattg atgtcaattg tttattataa
    13141 atatttgaaa gaaggtttca aaa
//
