LOCUS       CP049457               13164 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt245 plasmid pER06253.3A.2, complete
            sequence.
ACCESSION   CP049457
VERSION     CP049457.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331062
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13164)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 395.714231974921x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:22:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,856
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 45 of 80
            Pseudo Genes (incomplete)         :: 25 of 80
            Pseudo Genes (internal stop)      :: 21 of 80
            Pseudo Genes (multiple problems)  :: 11 of 80
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER06253.3A.2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            429..1046
                     /gene="cadD"
                     /locus_tag="FPP08_00210"
     ARG             430..1043
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1065..1412
                     /locus_tag="FPP08_00215"
     CDS             1065..1412
                     /locus_tag="FPP08_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNY35231.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1569..1661
                     /locus_tag="FPP08_00220"
     CDS             1569..1661
                     /locus_tag="FPP08_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNY35232.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1833..2804)
                     /locus_tag="FPP08_00225"
     CDS             complement(1833..2804)
                     /locus_tag="FPP08_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNY35233.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            2928..3347
                     /locus_tag="FPP08_00230"
     CDS             2928..3347
                     /locus_tag="FPP08_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNY35234.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPLTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(3873..4718)
                     /gene="blaZ"
                     /locus_tag="FPP08_00235"
     ARG             complement(3877..4718)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4825..6582
                     /gene="blaR1"
                     /locus_tag="FPP08_00240"
     ARG             4826..6579
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            6572..6952
                     /gene="blaI"
                     /locus_tag="FPP08_00245"
     ARG             6573..6949
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            7216..7794
                     /locus_tag="FPP08_00250"
     CDS             7216..7794
                     /locus_tag="FPP08_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNY35238.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(8155..8897)
                     /locus_tag="FPP08_00255"
                     /pseudo=""
     CDS             complement(8155..8897)
                     /locus_tag="FPP08_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            9323..10306
                     /locus_tag="FPP08_00260"
     CDS             9323..10306
                     /locus_tag="FPP08_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QNY35239.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            10299..10615
                     /locus_tag="FPP08_00265"
                     /pseudo=""
     CDS             10299..10615
                     /locus_tag="FPP08_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            11162..11311
                     /locus_tag="FPP08_00270"
     CDS             11162..11311
                     /locus_tag="FPP08_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNY35240.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(11479..12171)
                     /locus_tag="FPP08_00275"
     CDS             complement(11479..12171)
                     /locus_tag="FPP08_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNY35241.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            12281..12476
                     /locus_tag="FPP08_00280"
                     /pseudo=""
     CDS             12281..12476
                     /locus_tag="FPP08_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             complement(3874..4718)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          10672..10819
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(8156..8897)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9324..10306
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10438..10607
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 gaattttaga cctgtaaagc aataacaatt aggtctacca ggtagataaa gtgtacaaag
       61 tagaaccgaa aaagttattt attttgaaga tttttcgtag atataatttg ctaataaact
      121 tcctaaaact gaataaagaa aaatagttaa actcatgtaa tcactccata attctgaagt
      181 gtctccatgt ctacgcatag acacgcattt atgccgaaaa aatttattga tgttgagaag
      241 aacccttaac taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatt
      301 cgacaagttt tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata
      361 aagcacctat gattatttaa aaatataagt tataatacat tcaaaaatat ttttgaatga
      421 ggtgtattat gattcaaacg gttgttgctg ctgctgttct ttatattgct acagcagttg
      481 atttattagt aattttatta atattttttg ctagagcaaa gactagaaaa gaatatagag
      541 atatttatgt tggtcaatat ttagggtcta ttattttaat attagttagt ttgtttttag
      601 cttttgtatt aaattatgtt ccagaaaagt ggatattagg tttattgggt ttaataccaa
      661 tttatcttgg aattaaagtg gctatttatg atgattgtga aggagaaaag agagctaaaa
      721 aagaattgaa tgaaaaagga ttgtctaaat tagttggtac ggttgcaatt gttacgatag
      781 caagttgtgg cgccgataat attggtttat ttgttccgta ttttgtgaca ttaagtgtta
      841 ctaatttatt acttactctg tttgtctttt taattttaat tttcttcttg gtgtttactg
      901 cacaaaaatt agctaatatt ccaggaattg gagaaattgt tgagaaattt agtcgttgga
      961 ttatggctat tatttatata gctttaggtt tatttattat tattgaaaat gacactattc
     1021 aaacaatttt aggatttata ttttaattta gggtgtgatt ttatatgagt tatgaaaata
     1081 cttgtgatgt gatctgtgta catgaggata aagttaacaa tgctttaagt tttttagaag
     1141 atgataaatc taagaaatta cttaacattt tagaaaaaat ttgtgatgag aagaaattga
     1201 aaatcatatt atctttgatt aaagaagatg agttgtgtgt ttgtgatatt tctttgatat
     1261 tgaaaatgag tgttgcttca acttcacatc atttaaggct tttatataaa aatgaggtac
     1321 ttgattttta taaagatgga aagatggcat attattttat taaagacgat gaaataagag
     1381 agtttttctc taaaaatcat gagggttttt gaaacgagtt tcttcttgtc ttgatactat
     1441 aaaaaacaaa gttatataac ataagacgac ttatcggaag tagatatgct tgtatattca
     1501 cagatggtat aataatagag tcgcctatct ctcaggcgtc aattgacgaa gagaggaggt
     1561 gcattacttt gctaatcatt ttcgttcaca tcatagcacc agtcatcagt ggctgtgctg
     1621 ttgcgtatta tacttattgg cttagtaaac gcaacaaata attaataggc gactttataa
     1681 gccagcatcg aaaaaatccc cacactactg caatagtgtg gggattggtg cattaattgt
     1741 taatcatttt cgttatataa attataacac tggttagtag agaaatgcaa aatgattgaa
     1801 ttaatagtaa atgtattaat gaatactcac acttatactt ttaaaatagt tcttcctaaa
     1861 tgttgtcctt ttaaaagctg atctatcgtt ttatagaatt cttcaaatcc aatttcattg
     1921 tatagaagtg aatttcctat attccattca tttgctaact tactccatat atcttcacgg
     1981 atattatgat caacatatac agaatcaatg ccaattaatt taatacctct aagaataaac
     2041 ggtaatatat tagcgttaag tgaatttcct gctacatttc cacatacagt agcaacacct
     2101 tgataattta gacgttttaa accgtataat gttacattac tccccactgt atctaagaaa
     2161 taatcaaaaa tacctttttt aagcggttta ttatcatctt caattattat tacttgtttt
     2221 gcacctaatt tttttgcgat ttctatttgc ttttcttttc ttacgatagc agtgatattt
     2281 tcatatccaa ttttatgtaa aatttgtata gcaacactgc ccacaccacc tgtagcccca
     2341 gacactaaaa tttcaggttg tgatttgata ttcattccat atttttctag ttcaaaaatt
     2401 gataatgctg ccgtaaaacc ggctgtgcca tataccatag actctttaat agacatttct
     2461 tggggaatag gaacaatcca ctctccaggc acttgtgcat attctgaaaa tccacctgta
     2521 tgtgtcacgc caacatcata accagtaact aatacattat cgccttcttt aaacttatca
     2581 ttcgaacttg attcaacaat acctgcaaaa tctataccag gaatcatagg ataatctcta
     2641 attacaccgc ctttatattg taaagctagc atatccttat aatttattga agagtatacc
     2701 acttttacta atacatcacc cttcgataaa tgagattcat caactttttc tactttataa
     2761 ctaacatttg atgcactttt tctcactact accgcattga acatttatta gccttctttc
     2821 acaattgttt tgtagaagta tgtttttgtt gcaaaactat tttcctacaa aacttatcat
     2881 gatatgataa gcgagtcaat actaatgatt taagagaggt tttagttgtg gaattagata
     2941 attgtataaa ttatttgttg agtacttctc agaatagagt ttttaaacat tttaaaagtt
     3001 tattaagtcc ctataatatt actccagcag aatatggtgt tttgaattgc ttgattaata
     3061 gtaatttgaa tacacctaaa gcgattggta atactttaaa tttagaacct ttaacaatat
     3121 ctggaatatt agataagatg tccaaaaagg atctaataac acgtcaaact gataatgaaa
     3181 atagaagaac tgtgctaata aacagtacaa ataaagcaaa agacttatgg ccaacattag
     3241 aaaagaaagc ctatcaactt aataatcatt acttcgctaa tttaactaat gaagaaactg
     3301 tagtgtttaa aaagatatta ctaaagataa ataaaactac gttttaattt ttacgttata
     3361 aaaaagttga agtgcgaatt ataatcgagt aaatataaat gatagcttag cttaattata
     3421 tattaaagcc aactatactt ttattcccga aaaacctact atccaaattt ctaagatact
     3481 ttagcctcta taataaacat attgtaatat aatttatgta ttatttaggt ttaccctaat
     3541 cgaacaataa aaaatacttg taaaatgcta ttgtcacttt gcttgatata tgagaattat
     3601 ttaaccttat aaatgagaaa aaagcaacgc actttaaata agatacgttg ctttttcgat
     3661 tgatgaacac ctataattaa actattcatc tattatttat gattttttgt atatacaata
     3721 tttctagttt gttaaagaga attaagaaaa taaatctcga aaataataaa gggaaaatca
     3781 gtttttgata tcaaaattat acatgtcaac gataatacaa aatataatac aaactataag
     3841 atgttatcag tatttattat gcatttagaa tattaaaatt ccttcattac actcttggcg
     3901 gtttcactta tcaacttatc atttggctta tcacttttat tgtctttatt cgtaaaaatg
     3961 actaaaacaa taggttcaga ttggccctta ggataaacaa aagcaacatc atttctagaa
     4021 gcatatgtta ttgcttgacc acttttatca gcaaccttat agtcttttgg aacaccatct
     4081 ttaattaaag tgtctccgtt tttattatta aacattaaat caagtaagaa atttttattt
     4141 tttttgctta attttccatt tgcgataagt ttatttaaag tcttgccgaa agcagcaggc
     4201 gttgaagtat cttttttgct ctttggtgaa tagtaattta attctatctc atatctaact
     4261 ggatttgtta ctttatctcc tagttctttt agacgttgtt taactttttt gattccaccg
     4321 atttctttta taattttatt gtttgctgta ttatcactat atgtcattga agcctcaata
     4381 agttctttta aagtgatatc ttttcctaca tatttttcta aaataggaga ataagcaact
     4441 atatcatctt tgttaatatg tactttttta tttaacttat tataaggtac ttgttctaac
     4501 aaaatagcac tatttatcgc ttttgaagtc gaagcatagg caaatctctt atctgaatta
     4561 aattttactt ccttaccact tttagtattt aaagcataaa caccaatatg agcattatat
     4621 tttttttcta aatcatttaa ctctttggca tgtgaactgt ttgaattaca tgcacttaaa
     4681 actaaagcaa ttacaattaa aaatattaac tttttcaaaa taaaccctcc gatattacag
     4741 ttgtaatttt tataaatcaa taatattaca attgtaatat tgatgtcaat tgtttattat
     4801 aaatatttga aagaaggttt caaaatggct aaattactaa taatgagcgt agtaagcttt
     4861 tgttttatat ttctattgtt agtatttttt aggtacatat taaaacgcta ttttaattat
     4921 agtttaaatt ataaagtttg gtatctaact atgcttgctg gattaattcc ttttatccca
     4981 attaaattct cttttataaa atttaataat gtgaataatc aagcgcccac agttgaaagt
     5041 aagtcacacg atttgaacca taacataaat accaccaaac ctattcaaga gttcacaaca
     5101 gatatccata agtttaattg ggattcaatt gataatatct gcacagttat ttggatagtt
     5161 ttagttatta ttttaagttt taaatttttg aaagccttat tatatcttaa atatttaaag
     5221 aaacagtcac tttatctaaa cgaaaatgaa aaaaataaaa tagatacgat acttttcaac
     5281 catcaatata aaaaaaatat tgtgattcga aaagcagagg ctattcaatc tccaataact
     5341 ttttggtatg ggaaatatat tattttgatt cctagttcat attttaaaag tgtaattgat
     5401 aaaagactaa aatatataat actgcatgaa tatgctcatg ctaaaaatag agatacttta
     5461 catttaatta tctttaatat cttctctatt attatgagtt acaatccatt agtacatatt
     5521 gtaaaaagaa agataattca tgacaatgaa gtagaagccg atagatttgt ccttaataat
     5581 attaacaaaa atgaatttaa aacttatgcg gaatctatta tggactccgt attaaaaacc
     5641 ccattttcca acaaaaatat attaagccat tcatttaatg gtaaaaaatc attactcaaa
     5701 agtagattaa ttaatataaa agaagccgat ctgaaaaagc agtcaaaatt aattctaatt
     5761 tttatttgta tttttacttt ttttatcatg ataattcaaa gccagttttt aatggggcaa
     5821 tccttaactg attataatta taaaaagccc ttacaaagcg attaccaaat tttagatgaa
     5881 agtaaaaatt ttggttcaaa tagtggatct ttcgttatgt acagcatgaa gaaagacaaa
     5941 tactatattt ataatgaaaa agaaagtaga aaaagatatt ctcctgattc aacttacaaa
     6001 atttatttag cactgtttgg acttgaccgt catattataa gtgataaaaa ttcacgtatg
     6061 tcatggaatc ataaacatta tcttttcgag tcttggaata aggaacaaga tttaaataca
     6121 gcaatgcaaa attcagttaa ttggtacttc gaacgtatta gtaatcaaat accaaagaac
     6181 tatactgcgg ctcaactcaa gcaattaaat tatggtaatg aaaatttggg aagttataaa
     6241 agctattgga tggaagatag tttgaaaata tctaatcttg aacaagtaat agtttttaaa
     6301 aatatgatgg aacaaaataa ccattttagt aaaaaagcaa agaatcaatt atcttcttca
     6361 ttattgatta agaaaaatga aaagtatgaa ctgtatggga aaacaggtac aggtatagta
     6421 aacgggaagt ataataatgg gtggtttgta ggttacgtaa ttacaaatca tgataagtat
     6481 tattttgcta cacatttatc agatggaaac ccgtctggga aaaatgctga attaataagt
     6541 gaaaaaatat taaaaggaat gggtgtttta aatgaccaat aagcaagttg aaatatctat
     6601 ggctgaatgg gatgttatga atataatatg ggataaaaaa tcagtatcag ctaatgaaat
     6661 tgtagttgaa attcaaaaat ataaagaagt tagtgataaa acgattagaa cattaatcac
     6721 aagactatat aaaaaagaga ttataaaacg atacaaatca gaaaatattt atttttactc
     6781 atcaaatatt aaagaagacg atattaaaat gaaaactgct aaaacctttc ttaataaact
     6841 gtatggaggg gatatgaaaa gtttagtgct gaattttgcg aaaaatgaag aattaaataa
     6901 caaagaaatt gaagaattgc gagacatttt aaatgatatt agtaaaaagt aattagaggt
     6961 atgtttcaaa cataatacat atgtaaacca tacttgactc ttattaaatt agttttgtta
     7021 atgacattta aaatatatta ttaaagatga gagtgtccag aaaataacca ttttctgtac
     7081 actcttgttt tttgtctaaa taagtaagta ttacatagaa ttatgtgttc aaaaacccaa
     7141 caaaaaatct atgttcacaa aagacctcgt aatcaagtta tggtacacta aaggtgtata
     7201 taggaggtct gtattttgaa aataggttat gcaagagttt caactggatt acaaaatttg
     7261 aatttacagg aggatcgatt aaatacatat ggttgtgaaa agatatttaa tgaccatatg
     7321 agtggttcaa aaagtaagcg acctggttta gataaagcaa ttgaatttgc gaggtcagga
     7381 gatacgattg ttgtttggag attagataga ctaggacgga acatggagga tttaatcacg
     7441 ttagttaatg aacttaacga acgaggtgtg agttttcata gtttagaaga aaatataacg
     7501 atggataaat caagttctac aggacaatta ttatttcatt tattcgcagc atttgcagag
     7561 tttgaacgta atttaatttt agagcgatca tcagccggtc gaattgcagc acgtgctaga
     7621 ggccgatatg gaggtagacc tgaaaaatta aataaacaag atttaaactt acttaagaca
     7681 ctttatgata atggtacgcc aattaaaaca attgcagagc agtggcaagt ttcacgtaca
     7741 actatttatc gttatctcaa taaattagag gcaaaagaga ataaaaacaa gtagagcaac
     7801 taatctagct tactataatg aatctactaa ataattttat atatcattaa catacaaaga
     7861 ctttgattca atgtcactcg atgtaaaaga gacgttctga cagcgtctaa aatctcataa
     7921 tattttcctg ttatgagatt ttaataataa cacaagaaaa aagcgtacaa attcgtacgc
     7981 ttttttaata taatacctgt ttcactaatg aacgtttata acgcaatcat gatgagagta
     8041 ttaatacagt taattatttg aaatacttta gtttacataa tcgatgatta ccagaagttc
     8101 tcactatata aaagacacta aaattttata acttttagtg cctaatcaat tattttagct
     8161 accaaacaaa cgactccaaa accccttttt aggttccgtt tcttcactct tttcttctat
     8221 atcactatca ttgtcattta gattagctgg aacgtcttct ttttttgctt cttcaccttg
     8281 tgtactttcg tcgctttgac ttccagatat atccttatat tgaatctctg tttctttagt
     8341 ttcttcctga tctctgttaa tatttacagg attagtagta tatgtttttt cttgtgcatc
     8401 aacgttttgt ctagtgttag tagatgtatc aaagggataa ttaaggtgtt tttctttttc
     8461 taattgattt tctaattttt gaatcttttt attactctct aatgccagaa cttgctgatt
     8521 ttcaagcaat tttgatcttt cttgcaactc ttttctgtaa aattcaattg ttgtttcatg
     8581 acgactttct tgtttttcaa tttgattatt taaagtatca atctgatttt taaggatttc
     8641 tactatttga gcatcgcttt tcgattcaaa attaatatta tccgaccctt ttttttgttt
     8701 gtcttcagta gtactttcaa aaccatattt ctttttattt tcatttactc tttgaatgat
     8761 tttttctata tcggtatctt ctttaataaa cgaagtgttt tcctgtttta ttgtctctat
     8821 attcaatcgc ttaatattat taaaaatcgt ttgcttacta accctaattc ttcactcaac
     8881 tttttcacac ttttcatatc atcaaccctt tataaaatgt ttataaaccc ttgataaaat
     8941 attataagct tataatgctt aataataaag tgattaataa ataaaaataa acatccatca
     9001 acctttttat caagggttta tgaaaggttt atgatattta tcaagggttt ataaaccctt
     9061 gataaagcta tattttccct tttataagtt tatttaaagt gctataataa acttaatata
     9121 acaaaaaccc caactactgg taatagtcgg ggtttggtaa taaaacgttt agaaactgtt
     9181 tatttatata attttcgata cttgtattat ataatgaata atatcttagt gcaagcacct
     9241 aaatcattaa ataaatgtaa agctaaataa aaacgtttta ttacctctta ccaaactaaa
     9301 atatggaaag aggttttttg tcatgcaaaa tcaatatttt acagtgcaag aaaattacaa
     9361 agaaagattt tatcaaatcc ctaaagtatt ctttaccagt gaaaattata aaaatctaac
     9421 taatgatatg aaaatagcct atgctatctt acgtgatcgc ttaaatttat ctattaaaaa
     9481 tagctgggtt gatgaagatg gtaacattta ctttgtttat tcaaatgaaa agctaatgga
     9541 aatcttaaat tgtaaaaaag aaaagttaac aaaaataaaa aaagggttag aaaacgatgg
     9601 tctactcatt caaaaacgac gtggtttaaa taaaccaaat attctatatt taatgaaacc
     9661 tattgtcaca gaacgtgata tttacaaaat agaaaaagaa gaaaacgatg ttgaacctta
     9721 cggcgagaag gaagttcgaa aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag
     9781 ttcgaaaatc gaacgtccag aagttcgaaa atcgaacact aatgatactg actttaatga
     9841 cattgacttt aatgatactg agaataatga tatgaatgac ttgaatgata taaagtacaa
     9901 aaatgaaatt agtaatcatt caaatcattc aaatcaattt acacataatt ttgatgataa
     9961 agaaatgcta ttgcaagaat tcccagaaca actaactaat tatttattaa aatatgatta
    10021 tagagattta gaaatcataa aggctgttat tcttaaagca aaaaaatcat ttaattcaag
    10081 gcatgaagat atgcattaca tgttagagga cattgaggat gaaatattaa cttctttaaa
    10141 aagacttaaa aaagctatac atgatagggg agttaaaggc caaaaggaaa caatcaagtc
    10201 aatgcaagct tatttaatgc aaacaattct aactgaactt gaagaaacac atgcccttta
    10261 tatgcgccgt aaaaacatga aacaatataa tatctttaat caataattca aatgatttta
    10321 taacactcga agagacatca aaatattccg ttaaatcatc tgacttttta actgatgacg
    10381 acgaaaaaaa tcaagaaatt gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt
    10441 taagttatgg tggattgctt aaacaaaagc ataaaatttt aaacttagac gatgccgaag
    10501 atggtaattt gattaataca agtgatgaag ataaaacgac agacgaagaa aaaaagcaca
    10561 ttcaattacc gcaatttgga atttcgaaaa gcaaaattat tatttaagac attagtgata
    10621 actgatgtct ttttttattg tttttctgta aaaaagtgct gtcttatttt tgtgacaaat
    10681 gctgtatgta gtgtcacaaa agtgtgacac tacagctttg tatgatatca ctttaaaata
    10741 acttaaaacc cttggaatgt ctggccttgc cagatctatc atttttgaat gatagcaaat
    10801 ttcccttatg ctcttacgga gattttagag ttaaattaaa atttctaagt ttttgataag
    10861 aaaagccctg caggaattta gaaaaacatg gggaagtttt aaagtatttt atgctaattt
    10921 ttaaatttgc atgtaaatgg gcagtatcta aaaaattagt cactgatttt gctgaaattt
    10981 ttgtttgtga aattagcata tatttatttg gctcatattt gcgttttaag agactgtata
    11041 gatttttaag tacaaaacct atatgatttg cctctaagtc ttcaaaatgc ctcttaaaat
    11101 ccaaaataaa agtgtttaat attgtaataa ggattgctat ttttataaaa ggagagatat
    11161 catgaagatt aaaactttcg ctacttttac tcatcgtcaa ttagataaac aagttaatag
    11221 gtttttaaag aatgatattc atgtattaga aattcaaaca cacagtaatt ggttatatat
    11281 ttcagctatt gttttttatg aggataaata aggaacatcg tatttatgcc gaaaaaattt
    11341 attgatgttg agaagaaccc ttaactaaac ttgcagacga atgtcggcat agcgtgagct
    11401 attaagccga ccattcgaca agttttggga ttgttaaggg ttccgaggct caacgtcaat
    11461 aaaacaattg gaataaaatc aacctacaga gcatccagct gcaaatgcag ttccgcaaac
    11521 tgcttcacat acaccgccgg ctattggatt tacgattgcc catactccac agtagtgagc
    11581 acatgcataa agtccagcca tactacaagc gaatgctttg ccgttccatt tgaatcctgc
    11641 atatgttacc ggtctgtttt tatcaacttt ttcagcctta tattcaacgt aatttttgtt
    11701 aataagtatt tttccctctt tgttttcagt catacttctt ttttcggcaa tgaattttgt
    11761 gatttttttg ttgtagttat catatattgt ttcagaaact acaatttctt ttgttttttc
    11821 atttttataa gcgttattag ctatgtagcc atatgcattt ttatcatttg cttctgtagc
    11881 tagatatttt aatttagaat ttttgtcttc tagctttatt tttttaaatc cttctttttt
    11941 catttgcttt tcgaattttt gcatatcttc atttttattt tgcaattgtt ctagaaaact
    12001 tatttctttc ttggtcattt gtgatacttc gatgtttttg cttttcaatt tttctttttg
    12061 attttctagt aaagaacttt tttgttcttc atttgcgtaa gcattagaaa aattcgcgct
    12121 aaataccagt aggaacaagg aaattattga aaataatttt ataattttca tttgatacct
    12181 ccaagataat aagatatttt aattgtataa gtaagcgctt tataaaatca atatttattt
    12241 taatatattt ataaacatat ttttgaaagg agtctatttt atgaatgaaa atttagaaaa
    12301 atatataaaa agtttgccac ttttaggttt gttaattagt attttgttaa ttgttttatt
    12361 cttttttatt ttagatgttg atgaagattt attcgtggtt ttttttatac tgtttattgc
    12421 ctttgatagt taatttgtct atttatggtt tttatgtttt tgttaaaaga aagtaagatt
    12481 atgaataatt ttgtttttaa tttgtatgct ttgagggggt tggaaaacgg taggtgtata
    12541 gattagattt cttgtttttt tgaaacgagt gtgaacgagt ttcttcttgt cttgattttt
    12601 gataaaaacg ccctacagga atttagaaaa acgtggggaa gttttaaaga attttatgct
    12661 aatttttaat ttgcatgtaa atgggcagtg tctaaaaata gttattgttt ttgctcaaat
    12721 ttttgttttt tgaaaatagc atatatttat ttggctcata tttgcgtttt aaaagcatat
    12781 ataagtttta gatataaaac tatatgaatc acccctaaat ctttaaaatg ccctcttaaa
    12841 attcaaaata agggcataaa aaataaaagc taccccagat aaagtgaggt agctttttct
    12901 ttcatacggt ctatttattt ttctttcgac gccgtatcgt cgatgtaatt caaaaaagtt
    12961 atgggctata aatctacatc actttttcaa gaatgtagta gcaatattca acgcaaatta
    13021 atttttataa ctgctcggaa tatctcaagc cgtttcttta attttgaaat aaaaaaatcg
    13081 acgaaggtcg attacgtttt tgcacatgtc gatttatccg acgtataagt gcgcccttac
    13141 gggatttaac tagattataa cgac
//
