LOCUS       CP049442               15528 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt251 plasmid pER06541.3A.1, complete
            sequence.
ACCESSION   CP049442
VERSION     CP049442.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331068
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15528)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 112.853030216648x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:21:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,859
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,690
            CDSs (with protein)               :: 2,690
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 55 of 87
            Pseudo Genes (incomplete)         :: 15 of 87
            Pseudo Genes (internal stop)      :: 30 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt251"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER06541.3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            complement(685..1232)
                     /locus_tag="FPP14_00005"
                     /pseudo=""
     CDS             complement(685..1232)
                     /locus_tag="FPP14_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /name="5'(3')-deoxyribonucleotidase"
     gene            complement(1391..2448)
                     /gene="hisC"
                     /locus_tag="FPP14_00010"
                     /pseudo=""
     CDS             complement(1391..2448)
                     /gene="hisC"
                     /locus_tag="FPP14_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /name="hisC"
     gene            complement(2688..4201)
                     /locus_tag="FPP14_00015"
                     /pseudo=""
     CDS             complement(2688..4201)
                     /locus_tag="FPP14_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /name="ABC transporter permease/substrate-binding protein"
     gene            complement(4194..5170)
                     /locus_tag="FPP14_00020"
                     /pseudo=""
     CDS             complement(4194..5170)
                     /locus_tag="FPP14_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(5391..6990)
                     /locus_tag="FPP14_00025"
                     /pseudo=""
     CDS             complement(5391..6990)
                     /locus_tag="FPP14_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /name="RecQ family ATP-dependent DNA helicase"
     gene            complement(7002..8884)
                     /locus_tag="FPP14_00030"
                     /pseudo=""
     CDS             complement(7002..8884)
                     /locus_tag="FPP14_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /name="ABC-F family ATP-binding cassette domain-containing
                     protein"
     gene            complement(9184..10470)
                     /gene="ltrA"
                     /locus_tag="FPP14_00035"
                     /pseudo=""
     CDS             complement(9184..10470)
                     /gene="ltrA"
                     /locus_tag="FPP14_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            complement(11025..12963)
                     /gene="ltaS"
                     /locus_tag="FPP14_00040"
                     /pseudo=""
     CDS             complement(11025..12963)
                     /gene="ltaS"
                     /locus_tag="FPP14_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid synthase
                     LtaS"
                     /name="ltaS"
     gene            complement(13440..14445)
                     /locus_tag="FPP14_00045"
                     /pseudo=""
     CDS             complement(13440..14445)
                     /locus_tag="FPP14_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /name="biotin-dependent carboxyltransferase family protein"
     gene            complement(14429..15137)
                     /locus_tag="FPP14_00050"
                     /pseudo=""
     CDS             complement(14429..15137)
                     /locus_tag="FPP14_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /name="allophanate hydrolase subunit 1"
     gene            complement(15224..15430)
                     /locus_tag="FPP14_00055"
     CDS             complement(15224..15430)
                     /locus_tag="FPP14_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNW84267.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISFI
                     IVYALIRELMPFVVRAMDLMYHQ"
                     /name="hypothetical protein"
ORIGIN
        1 aagttgatat tgatggcgaa atttcattag aaacacctat tgatattgag gttataccaa
       61 atgccattca gttgcttact gtcaatgatt tgtaagcatc cagaatgata ttaacgcgat
      121 attatattag gtttaaagtg taataataag attcaacgta cctaatattc gttgttatga
      181 gctttaagct attatcctgt ttcaaaaaca gaaatataat tttggcgcat gccactaaaa
      241 tgattgatgt tgtattttaa atcatagtgg tttatgcgtc tttttcaaat tctataaaaa
      301 atcggatgac gtgtaatctg ccaaagatta acacattcat ccgatttata ataatgagat
      361 agactaacat ttattgagag cgggacggaa atgataaaga acgactaatg attgattatg
      421 tagcgattct ttatcattag tcacagctaa tgtgtactta aaaataggag ggagtgggac
      481 agaaatgata ttttcgcaaa atttatttcg tcttcccacc ccaacttgca cattattgta
      541 ggctgacttt tcgtcagctt ctgtgttggg gcccacaccc caacttgcat tgcctgtaga
      601 atttcttttc gaaattctct ttgttggggc cccagtatat gcatttttat ttgctacatg
      661 aaaatgcttc aagtgaatat atttttattt ctcaatatta tctacaaaaa tactgtttct
      721 accatctttc cagctattta cgcgttcaaa ttcgattatc attaatatta tgcactgctg
      781 taaacataat cggtgtacca gtaaaaattt caagctgtct aggattgtca tctattaaat
      841 aatcagcttt aacgatgttt ttttctacca caaaaaacaa aatgctgagg atctaagaat
      901 ggaaagaact ctagtaacca ttcatattta tcactgaatg atgttggtac atccattgct
      961 gctgtagcaa tatatacatc ataatgttca gttaattttt ttacaacttc ttgtgcatac
     1021 ggcatcactt taaggtgtct gaagaagcct ggttctctta atacttctgt aattaatcca
     1081 tcatgttcag gaataacatg cttaagtttt tgaccattca aagcttccat tttaatacct
     1141 aaatccgctc taaaattgac agcatcaatg atttctccta atgtatctgc caatacttca
     1201 tccatatcaa tcgcgattga tttacgggtc attttaatct tctcctcaaa atatcagtat
     1261 taattcagta tagcaaaaaa gcaattgata caaaacaata tcatctattg ttgcttttta
     1321 cactgaatgc tctcacctca tcattcacac tgcattttgt cacatattca taccatgtgg
     1381 tgcccactta ctattcgtat ttaaagtttg ataaaacttc taacatttta tcattttgtt
     1441 ctttaaaacc aattgtaatt ctaacaccag ttggaaatgg tcgcgtaata caccctacat
     1501 ttaaaagtgc ttcataaagt tcatttaccc gctttgtttt tacaaatata aaattcgttt
     1561 gacttggtat gaaatgctca ctttgaggta attgataaaa tctttcgcgt tcaacactat
     1621 ttttatgtgt tacctctttt aaatattgtt gatcttcaag tgctgcaact gctgcgtatt
     1681 cagatatacg tgtcacatta aatgggggtc taatgatgtt ccatttttca atcgcatgtt
     1741 cgcttgctac cacatatcct acacgtaaac ccgctaatcc atacgccttt gaaaatgtac
     1801 gtaataagaa tgcattgtca tatttttgtt gcaaagcaag tgtatccggg tagtcctctg
     1861 ctgtcacaaa ttcaaaataa gcttcatcta tgagtaccgg aacatgtgat ggtacttgag
     1921 ataaaacgaa tctaagctct catgattaaa atacgtacct gtaggattat ttggattaca
     1981 taaccacacc aatgccgtgt catcattaac ttcttttaaa atgccattta agtcgaagcc
     2041 accatctttt aaaggtactt gtatcacatt agctgattca acaatcgcat tgtgataata
     2101 ttgaccgaat gtcgcttcac ttgtaacaat agtatcccct ggcgttaata ctgctctaga
     2161 aatcattaat ataacttcat ctaatcccgc accaaataaa atgcgtgatt gatctacatt
     2221 taaatgttta ctaatcgccg cttttaatgt cggtgatcct gtttcaggat aataatataa
     2281 ctcatctaag tgtgctgata tcgcttcttt aactttaggc gatggtccat acaaattttc
     2341 atttgatgca agtttatata aatctccttc aatgccatac ttttctttca atgcccttgg
     2401 agataaacca ggctgatatg ctgatagttg attaagttgt tctttcataa taaaaacaac
     2461 ctccttagaa ttaactgaaa ttttaaaaaa cttatattca ttatatcgct ctatgtttct
     2521 aaaatgcaat gctactattt tcacatcata ctagctttca ttatgtttaa catgtcatat
     2581 tttttaatca ttattgcatg tttaaattga acataaacat agatgtaaat atagcaacgc
     2641 cacataagat ataatggtgt gcccctcatc ttatgtggcg ttggtcgtta tttgattaac
     2701 cctttttctt ttaaataatc tttcgcaact gtatatgggt cttcattttt aactgttact
     2761 ttatagttca tcatttgcat atcttcatca gatattttgt tttctagttt gtttaacggt
     2821 ttcttaattt ctggatgttt ctttaaaaag ctttctttaa ataatggtgc tccttgatat
     2881 ggtggaaata cgtgcttatc atcttttaac acaaccatat catattgttt taattcagcg
     2941 tcagttgaat atgcatctat taaattaata ttacctttat tgatcgcttg atatctcaac
     3001 ttaggttcca ttgtacgtat gttatctaaa tttaaattat aagccttttg aacagctttg
     3061 taaccatctg gacgatcatt aaattccaat gtaaatcctg gtttaagttg atctttaacc
     3121 ttatttaaat caccaattgt acgtatatta tgttgtttag caaaatcacg ttttacagct
     3181 aaagcatacg tattgttata tttcattggt tttaacatag tcatttgata tttcttttca
     3241 agactttgct tagcttgatc atatactttt ttctcttctt ttgacttcaa tggttctttt
     3301 gttaactcac ctaaaactgt tccagtaaac tctaaatacc catctatatc gtcagttttt
     3361 aaagcattaa ataaaaatgc tgttttgccc ataccatctt tcacttctac agtatttttg
     3421 gtctcttctt ctattaaaat tttatacata tttgtaataa tcgaaggctc ggagccaagc
     3481 tttccagcta acgtaatttt atcacctttt tgtgcaaaca taggaatagc aatggccagt
     3541 ataataatca ttactataac gcctaacgtc atcaataact ttttataaga taatttagcc
     3601 ataaatctta aaattaaatc aaatataatt gccaataagg ctgctggaat tgcacctaat
     3661 aatatcaatg atgcattgtt acggtctata cctaataaaa ttaaatcacc tagtccgcct
     3721 gcaccaatta atgctgctag tgttgctgta cctataatta ataccatagc cgttcttaca
     3781 ccagccatta taacaggcat tgctattgga agttcaactt tagttaaacg tctaaatggc
     3841 ttcataccta tacctttagc cgcttcaatg agcgatggat caacttcttt aattccagta
     3901 tacgtattcc ttaaaattgg taacaatgca tacactacaa gtgcaataat tgctggcaca
     3961 cgaccgatac caaataaagg aatcattaaa cctaataatg ccaacgatgg tatagtttga
     4021 agaattgccg caatattcat tacgatttca gatatcgttt tagtcttcgt caataaatac
     4081 ctaatggtac cgcaatagca gttgcaatca ataatgcgat aaatgatatt tgaatatgtt
     4141 ctatcattgt cgaaaatagt tgccccttac gttcactcaa tatgtcgaaa aagttagtca
     4201 tgttgagcta cctccttttt ctgggacaaa tatttgaaga tatctttcct ttcaataaca
     4261 tattgaccta cgctatcttc ttgcatgaca atgacacgct cgctctctga taaatgttga
     4321 tacaatactt caatttgttg atcgtcataa acaattggat aagcgctcat agatgtaacc
     4381 tcatcgattg gtttcataat atccaagtca cggataattg ggttctcttc aacacatggc
     4441 gcatcatctt ctaaatgact acccataaat tgtttaacaa attcactttg aggattattt
     4501 ttaaatcctt ctggtgtgtc aatttgttca atatgacctt cattcaaaag acaaatctta
     4561 tcaccaagtt tcatcgcctc ttgaatatca tgtgtaacaa atatgattgt cttcttaatt
     4621 ttagtttgta attcaattaa atcgtcttga agtttttctc ggctgattgg gtctaatgca
     4681 ctaaacggtt catccattaa aataactggt ggatcagctg ctaacgcacg tataactcct
     4741 acacgttgtc gttgccccct gacaattcat caggttttct gtttttatat ttttcaggtt
     4801 ctaatccaac catatcaagt aattcatcta ctcttttatc tatatctttt tctttccact
     4861 ttttcatttg tggcacttgt gcaatatttt ctttgattgt catatgtggg aataatgcaa
     4921 tctgctgcaa tacgtatcca atatcccaac gcatttcgta tactggataa tcacttattg
     4981 gtttatcttt aaaataaata taaccttcac ttaagtgaat gagtcgatta atcattttta
     5041 atgtcgtagt ttttccacaa cctgaaggtc caattagcac aaaaaattca ccctcattaa
     5101 tattgaaact aatgttatcg acagcaacat gtttgccata acgcttagtt acatttttaa
     5161 acttaatcac tttgccacct ctttttttct catagcataa aaccgagatt atatgtatgt
     5221 attccctatt taaccacgtt tattacaatt ttcaaattta aatgatttat ccttgaactt
     5281 ttttaacaaa ataatgaata ataggtaatc tccagttaag aaatagtgtt attttacttt
     5341 aaattcaaaa aacacccagt aaaacaagga atgtttacta ggtgccttca ctatactttg
     5401 gctttataat tttgaatcgt ttctaaaaat gctggacaat aatgttttaa tttgtaacta
     5461 cctacgccat caatattaat catatcttgt ttcgaagcag gcttacgttt agcaaattcc
     5521 tctaacgtgt aatcagaaaa tatacttaca ggtgctatcg ttaatttgtc acttaacttt
     5581 ttacgaactt ctaccaactg actgaataat gctcggtcaa ccccttcaac cgtatttata
     5641 aatacttttt cagtcgcttt ttgcttaaat ggtgttgtga atacttctac ttcattactg
     5701 agtaattttt taattgaagt atcacacatt aatatttcgt cattttcatt taagaaccct
     5761 ttgaatctta attcatctat taagtgactt aattctgatg tagtgtaacc tttcattaaa
     5821 ccatgggttg aaatttgatc ataaccttta tacttaatat aatctgttga ctctcctctt
     5881 aatacttgaa tgataacact ataactctct tgttgtttca tacgagcgat gcaactaata
     5941 atcatcttag cttcttgtgt catattatat gatttatctt gttgaacaca attactacat
     6001 tgttcacatt cttctaattt ttcattcggt tcaaaataat ggacaattgt tgcttataac
     6061 atttttttgt tcgtaaaaca aaatttattt tcattcaaca tttctaattt atcaacaaaa
     6121 tacataaatt catcaataaa ccaaagcatt caacgcacta ctttaacaca tcaaagaagt
     6181 cattctgcgc ttcttctctt tctttattgc tcaaacctgc atgatatata acactatcaa
     6241 ttttctggct ttctaaggct tcttgaagct cttcaacttg cttacgtgta gaacaataaa
     6301 tatacctgca tcttcatcgt gtgttttaat ataatctaat acaaattttt gccgttgata
     6361 agtaggattt actttaaaaa ttaagtttct acgcttagta ctcgttttaa tttgatcagt
     6421 ttgagcgata tttaactttt ctctaatatc ttgctgtact tcaactgttg cagttgctgt
     6481 caacgctatt attgtaaaat cttgaggtaa cgtaaatact ttagaaatga cgttttggta
     6541 actcggtctg aaatcatgac cccattttga aatacaatgt gcttcatcaa acgccactaa
     6601 atgaatcttg atacgttgaa gcaaatttaa aaaatatcga ttttcaaatc gttctggtgc
     6661 aacatataaa aattgaattt ctccatttga taatgctttt tcaatacgtt gttgctcttt
     6721 ttgagtcaaa ctactattta aaaaagcagc ttgaattccc atcgctttta attgatccac
     6781 ttgatctttc attaatgata ttagtggact tattacaatt gttgtaccac ctaacaataa
     6841 acctggtact tgatagcata tagacttacc tccaccagtt ggtaagacac caagcacatt
     6901 acgatggtct aatactttgc taataatctc ttcttgtcct ggtcgaaacg tttcataccc
     6961 aaagtaatgc gataatgttt gttgcatcat aaattgaccc cttattgttg ttctttaatt
     7021 tcttctaact cactccatct tgtgatgtct atatcatatt gaatttcaag tcgttctttt
     7081 tcttcgttta attctttaat tttcccataa tctgcacttg cctcaatcat gagcacatca
     7141 atttcttcca ttcttacttc cgcttgttct atacgtttca tcaattgttc atattctaat
     7201 ttttctttat atgataaccc atttttctta cgtacagttg tagaagactt agattgttgc
     7261 ttcaatgtgg atttattttt atctaatgat tttttataac tttcataatc ttcaaaagtt
     7321 ccgataatct tttccatctg accatcatga ataaaccaat atgactgtgc aactttatta
     7381 agaagtaacg atcatggctt acggtaatca ctgtaccacc aaaagtatga atataatctt
     7441 caagtatagt taaagtctca gtatctaaat catttgtcgg ttcatctaac aacagaacat
     7501 ttggctggtg tacgagtaga cgtaataaat acaaacgctt ttgctctcca ccagataatt
     7561 tataaacttt tttaccatga gttgcacttg gaaataaaaa tcgttcaaga agttgtgtaa
     7621 tagataccga ggttccatct ttttcttttg cgatctcact ttcttctctt aaataatcaa
     7681 tcatacgaat atctctatcc agggtctcat ctgtttgctt aaaataagct actttaaccg
     7741 tctgcccagt cttcaactta ccttcgaatt gttggtcttc tccactcaaa atatttaaga
     7801 gtgttgtttt accagctcca tttggcccaa caacaccaat acgctcacct ttttgaataa
     7861 tttccgtcag atgttcaaat aatactttat cattaatagc ctttgataag tcttctaatt
     7921 cgaacacttg cttacctaat cttgaataag caagattcaa ttcaccttta tcgtctttat
     7981 attgctgatt aacttcattt tctaggtcat taaatcgatt aattctagct tgttgctttg
     8041 tagtacgggc cttagctcca gccctcatcc aagcaagttc ttccttatat aaagctcgtt
     8101 gcttttgttg ttgcttttga agtgttactt ctctttcagc gcgcatttca atataagatt
     8161 catagttacc aggatatgac gctaacttac ctctgtttag ttcaataatt ctagtggaaa
     8221 cttcatttaa aaaatatcga tcatgggtta cgaataaaac tgtatgagga tattgcttca
     8281 cataattgat caaccagctg attgattcga agtctaaatg gttcgtaggt tcatctaata
     8341 acaataaatc tggttgttct attaatgttt tagctagtac aacacgtttt tgttgtccgc
     8401 cggataattc tttaatgtat ttagtagtat catgtatacc tagttttgag aggatcgttt
     8461 taatttcagc gttatagtcc caagcatcat gttgatccat agcatcttgc gcatccatca
     8521 ttcgcttgaa caatttatca ctttggtcat ccgcataagc ttgcactgct tgctcatatt
     8581 gcttgatgat gcgtaaagtt gttgtatcag aacttaatac tgcatcaaaa acagtcatat
     8641 cttcattaag atcctgtttc tgagacgaat atcgaattcg atattggttt ggatgcataa
     8701 cattggctgt aaaatcatca tcaataccac caattacttt taacaacgta cttttccctg
     8761 taccatttat gcctactaaa cctatttttt caccttctga aattgataaa tctaggttat
     8821 cgaatatagt cttatcggca taagatttat ttaaatgttc aattttatat gcttccatac
     8881 ccatcttcct tcttctgttg aaatctctac gtacttaaat cagttctata cattatacac
     8941 ctttttgaaa tcaaaagaaa tcgataccgt taagtgtcat aatttaatat attgttgctt
     9001 caactataaa taaaacttga taaggctttg atgactatgc taatgatctt tataatatat
     9061 ctttttatca actcatttaa tgtaaatcat aatataaaag aacataaatc gagtaggaga
     9121 ataatcatta cttgattatt cgtcctctca caccaccgag cgtacggttc cgtactcggc
     9181 ggttcaatat cttgcgtaag ccgtctctgc gagttgggtt agtgatagta acccccactt
     9241 gtggagacgt tttgttgtaa gtgcacgatg aacctcatgc gtagatgata aacgccagta
     9301 tttctttctt gagctcgcga ttctcattgc gctatcgtgg tctagaccat acttgcgcaa
     9361 catcttatat tttgttttag tcttcttcca tcttttgagt atgagttgcc taatgcggcg
     9421 atttagccaa ggttctaatt cttgaagaaa tctttttatg aatccttttc caaaatagtt
     9481 aatccatcct cgtgtcacac gattaatttc tgttataata tcttggaatg aacctggtct
     9541 acttcgtctg gttaaccact ttaagttgcg ttttaaattt ctttttgctt ccgtagtggg
     9601 tctgaaacga taagtgccat ttactttgct cattagacaa ctcaagaact ttaaacgggt
     9661 aggagaaccc accttacttt tatcattatt gacttcaagt ttgagggttt tgtgtatgaa
     9721 tgttttaata ctatccatca catgttcacc tgcacgtttc gttttgacaa agatgacgaa
     9781 gtcatctgca tatctcacaa aacgatgatt acgtttttcg agctctttat ccagttcatg
     9841 tagatatatg ttacagagta atggtgatat cacaccacct tgcggtgcac ctatctttct
     9901 ttctgcgact tcgccagata ggtcaatggc accaacttgt aaacttctac ggataaatgt
     9961 tgatacagct taatctttga tatggcgctc aaataaatac atgagtttat catgatttat
    10021 gttgtcaaag cattgtttta aatcacaatc tacggctatg gtataacctg cttcatagta
    10081 agacgcacat tctttcagtg ctgttcctgt actacgatta ggtctaaagc catgactgtg
    10141 tttagagaag gtacgatcga tactaggttc aatcacttgt ttaattgctt gttgtatcac
    10201 tctgtctctt acgacaggta taccaagtac acgctttttc ccatttgctt tgggaatttc
    10261 gactttctta actgcttgag gtttgtatgt accttcaagc agtttttgtt ttatttctgg
    10321 aaagtactgc gcaaagtgac cttgtatttc actcactttc atgccatcaa tgccaggggc
    10381 acctttattc tttttaactt tcttaattgc cttttgaatg ttgtgttctc ttacaacaag
    10441 ctccatcatc gatggagact cacgatacat ttctttcatt tcacctaaga tttaccatgc
    10501 gcacttattt gccctttttt cgttccacta cttatctctc cataagttgc caactagttc
    10561 gttgttttct gcatgatgta aatctataac ctccattctt tactttgtat gaatattgtt
    10621 ccgtccttcg gtacattttc cctactatga cgtctgctga cttctcatta ttcgttgtta
    10681 ctacggtatg cgcaccgcta atgagacctc cctgggtaag gtgcgaccac tttccactca
    10741 tctcatcgca tcatttaccg tataaaattc gggtagtatt ggacttcatc ttgtttagca
    10801 gactcatccg ttttatacgg ccttattatg atgtttctgt tcgtcaatgc gagtgtttgc
    10861 gtccgacttc cttcagattc cacttcacaa tggacaccct tgtctttcgc taacagttcc
    10921 tactaccaag cctgtaacgg actttcaccg tcaagttgtt gcccatgcca ggcgcactaa
    10981 aaacccggat tgatatttta tcaatccggg ttcgtgttta aatattattt tttagagttt
    11041 gctttaggtc ctgtttcata tttatacttc gaaggattta cctttttgaa gtctggattt
    11101 tgtagaatct aaataaatca ccattaagca cgttgtcact catttcgaga tccttttcaa
    11161 cttgcttttt attcttttcg aaatcagctg gttgagtagt tatgagttca ttatttttat
    11221 tagaataaat cttaccatta acatatttat aatcttttgt tataaagtca ccatttctga
    11281 atggaactac ttgattatga cctttagaga ataaatcagt accgaacatt aaatagttct
    11341 tcgtatctat accagccaaa tgtaaaattg ttggcattac atcgacttga ccagcatatt
    11401 cattattgat accaccagat ttaccaggga ttttaatcca gaaaccagtt ctgtttaaat
    11461 ctgtaaattt agctggtgtg attttttcac ctaatagttt ttccatggca ttgttatggt
    11521 tttcagagat accatagtgg tcaccataaa tcataatcac tgaattgtca tataatcctt
    11581 ttttcttcaa gtcattaata tattcttcta atgcttcgtc taaataacgt gctgtttgaa
    11641 tataaccatc aactgttgca tcacctgtgt ttgacttctc aatagttgca tccttttcat
    11701 ctaattgaat ggatagtgtt agtcaatgta attaaatgag aatagaatgg tgatttcatc
    11761 ttagcttgat aattagcaga atctttaaag aaaattttgt ctttcaagcc taagtttaca
    11821 acgtttttat ctgacatgtc atagtatgtt gcatcataga atttatcgat accaaagtgt
    11881 ttataaactt ggtctctgtt ccagaatgtt ttatagtcac cgtgcatgac atcagatttt
    11941 gtagccttgc ttttgatcta aaattgctgg taatgactga tacgtattat ctccttttaa
    12001 tgaaaggcag aaccttgcgg taaaccgtat aaaactgtta tccattgtaa attcagagtc
    12061 agatgtttta ccttgacctg tttgatggaa aaagttaggg aagtatgtga attgctcttt
    12121 cccacttgat aatttgttta aaaaacggtg ttacttcttt tccattaacc tttttattaa
    12181 ttaagaaggt ttggaaactt tctaaatgaa tcttaataat atttttcttc tttgccaccc
    12241 cataatattc tgggttaggc tctgtttgac gttgcttcgt ataattaata cttttgttaa
    12301 gtcatcttca gatgctaacg ctttttgttg attattttcg atagtctaac accatcgtat
    12361 actgtaaagt tatacggtcc taaatatttc actaaatatt tatggtcaaa tgtacgtgtt
    12421 aataattctg gtctgtcagt ttcagcaaaa gctaagttta agaagaataa tgctactgaa
    12481 gccgccatta cgacaggaac aaatttctta ctaaatgctt ttgtgtctaa ccatttttgt
    12541 tttaaatatt aaaatgaata agtaaactaa cgtatcaatg aaataaacaa agtcatacca
    12601 tttgaatgat gcactaaccg caccacccat agattctacg ttacctactt ggtttaaagt
    12661 actaaacgtt aaaaaatccg agaagaatct aaagtacaca acattggcat ataataggaa
    12721 cgtcataaga agccgcctat gaacatgaac caaaatgctt ttttgccttt aaagaatagg
    12781 aacacactta aaaccagtgc tactaaacta taaggattca tcaataatat taagttttgt
    12841 actaaacctt taacacctaa agaaaaaatc aacataataa gaaaaatacg tcttcaaggt
    12901 aatcgttatt acggttaata agaagaacgc aaaaagacta attttctttt tttgtgaact
    12961 catgattctt tcccccgtta tttagataat aaatcttgcg aaagtacttg aataaattag
    13021 atattaacat tatgtagtta attaataagt gcgtttaaaa gttaacattg tcataattat
    13081 agtaattact ttcagattca agtttataat tttcattatt tgtagctttc aacttttgat
    13141 ttaacaagta ttttgggcta caactcataa ttgcttgtgt aagtttcttt atttaaaatt
    13201 atcattcaat taaacaaatt taaaatttgt aatcaaattt gtaataataa gcatagctca
    13261 attaataaat atagagtaat tttagtcaaa attcaagtaa aaacacactt tatcttttca
    13321 ttttactgtg aaacgaatta gtatatgata catttcgcag ataaaacaca gtattatttc
    13381 attatacatt aggaggaaat tatttgcatc ggactcgagt atgagctacg taccaaatat
    13441 tatttcatca tttctaattg cattctatat tctattccat ggaataattg gtttaaccag
    13501 ttactatact taacaaggtt cttttcagcc gtttaaaata tctataaatt gaaatttcaa
    13561 acgtgatcct tgcggttttt gtgctaattt cgttaaatga taacttgcga ttgtaccgat
    13621 ttgcgggtag ctacctagcg tgtaaatgat cattttaata agataatagg tgtgccatca
    13681 cgcttaattt gtatcgttcc ttttttgaca gtctgatacg gtggcatatc ttcataaaaa
    13741 agctttgatt tttttcacct tcaagcatca tgcccatgcg atttgctttg ctcgttactt
    13801 tataatcatg tttcacaaat ctttgaatgg catcttcttt aaaatcttcc gtacctttat
    13861 ttttaacgac atgaaataca tcagacatat aattaaatga caaggcgtac ccatcaatgc
    13921 cccaatctgt ttgtttcgtg tgagcaaggt tttcaaataa cttatgatga cgagctgtat
    13981 aatctctctt aagctttatt tcatcgccat cttgtaatgt tcttccttta aaaccaattt
    14041 ttacattaaa gtcggttgaa ttagatccta accatgcatc taattcaaat ccacctccca
    14101 cagctaaata cactcgcgat gtataacttg tttctctaaa ctttaaaaca tcgcctttat
    14161 ctaacaaata caatttatat ggagatatag tcatttgctc agtgtaagct ttgacattac
    14221 ccctgctaat gcaatcagcg taggttctgt aaaacgaatc gttgccattt tattagtcat
    14281 ttccaaagtt gcttcattct tgtcatttgc aactaatcga ttagcaattt catgtgctaa
    14341 agtatcaagt gcaccacatg gaattacacc atcatgttca tatccccttc taccaaagtc
    14401 ctgaaagcta ctgaataagc cacttttttc aattatgatt gacatcttta aacgctccta
    14461 cttctaattc attttcattg ataggtctaa atttaatatt atcgcctaac tttaagcgtg
    14521 cgaaatctga ttctttcgga ttaaaaagtg ataatggtgt ataaccaata actaaccaat
    14581 cattaattgt atctgtagtt acaataccgc atttttccct tcaagtacta cagaaccagc
    14641 cggaatgaat tttttctgtt tactcgtgtg attaatatac aatttcttat ttaatccagt
    14701 taaataagga aacccaggtg aatatcccat catcgaaaca aaatattgcg ccttagaatg
    14761 taattcaata aatgtttcta gcttgatttt gtaatgtttt aaaagtgatt ctaaatccgg
    14821 accatatttc gaaccataca caattggtat ttcaatcaaa tcttgctggt taaaatcatg
    14881 ttttatgttt aaatgaatcg attctattag tgctttcatg tataaaaaag gtgattgtat
    14941 attatggtgt ttaatcatat cccttgcgtc atagacaatc atcatgtctg attctgatgg
    15001 cacaatttct ataataaatg gataattttg ttcaattaaa tatgagcgaa gtgttaataa
    15061 atcttcggtt aaacttttag atactttttt tcaattgcga ctacaatggc ttggtcacct
    15121 tgactatata ttttcatttg tacacctctt caatatttgg ttactttact ttgattttta
    15181 attatagaat cgattgaata gttcatttac ctactcacta aatttattgg tgatacatta
    15241 aatccattgc tcgcacaaca aaaggcatga gttcgcgaat taatgcgtat acaatgataa
    15301 agcttataac agtagtaatt gttgctatca aacgaacaac atatattcta ttttcagata
    15361 gcaatcgatt cactaatcga taatttacgt atacaagaat taacaacagc acaatataaa
    15421 caataatcat atttgcccca tttaattttt aaatttgttt tcacttcatt ttctacatta
    15481 ttatcatact aattttaaag gcaaaggtgg acatcggcaa cctctcgt
//
