LOCUS       CP049428               13163 bp    DNA     circular BCT 24-SEP-2020
DEFINITION  Staphylococcus aureus strain pt259 plasmid pER06916.3A.1, complete
            sequence.
ACCESSION   CP049428
VERSION     CP049428.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN12331076
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     PathoSPOT genomic surveillance reveals prolonged under the radar
            outbreaks of nosocomial methicillin resistant S. aureus bloodstream
            infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caban,A.B., Pak,T., Kumaresh,A., Dupper,A., Chacko,K.,
            Sullivan,M.J., Altman,D.R. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223.873263549416x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/28/2020 16:21:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,902
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 43 of 76
            Pseudo Genes (incomplete)         :: 24 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="pt259"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER06916.3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2016"
                     /collected_by="Mount Sinai Hospital"
     gene            join(12817..13163,1..499)
                     /gene="blaZ"
                     /locus_tag="FPP22_00005"
     CDS             join(12817..13163,1..499)
                     /gene="blaZ"
                     /locus_tag="FPP22_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QNW62137.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="blaZ"
     gene            complement(1025..1444)
                     /locus_tag="FPP22_00010"
     CDS             complement(1025..1444)
                     /locus_tag="FPP22_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QNW62138.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            1568..2539
                     /locus_tag="FPP22_00015"
     CDS             1568..2539
                     /locus_tag="FPP22_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="QNW62139.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(2711..2803)
                     /locus_tag="FPP22_00020"
     CDS             complement(2711..2803)
                     /locus_tag="FPP22_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QNW62140.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2960..3307)
                     /locus_tag="FPP22_00025"
     CDS             complement(2960..3307)
                     /locus_tag="FPP22_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QNW62141.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3326..3943)
                     /gene="cadD"
                     /locus_tag="FPP22_00030"
     ARG             complement(3330..3943)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5059..5253)
                     /locus_tag="FPP22_00035"
     CDS             complement(5059..5253)
                     /locus_tag="FPP22_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNW62143.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            5363..6055
                     /locus_tag="FPP22_00040"
     CDS             5363..6055
                     /locus_tag="FPP22_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNW62144.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            complement(6223..6372)
                     /locus_tag="FPP22_00045"
     CDS             complement(6223..6372)
                     /locus_tag="FPP22_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QNW62145.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(6919..7235)
                     /locus_tag="FPP22_00050"
                     /pseudo=""
     CDS             complement(6919..7235)
                     /locus_tag="FPP22_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(7228..8211)
                     /locus_tag="FPP22_00055"
     CDS             complement(7228..8211)
                     /locus_tag="FPP22_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QNW62146.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            8637..9380
                     /locus_tag="FPP22_00060"
     CDS             8637..9380
                     /locus_tag="FPP22_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QNW62147.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(9741..10319)
                     /locus_tag="FPP22_00065"
     CDS             complement(9741..10319)
                     /locus_tag="FPP22_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QNW62148.1"
                     /translation="MKVGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(10583..10963)
                     /gene="blaI"
                     /locus_tag="FPP22_00070"
     ARG             complement(10587..10963)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(10953..12710)
                     /gene="blaR1"
                     /locus_tag="FPP22_00075"
     ARG             complement(10957..12710)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             3..499
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="95.76"
                     /coverage="58.72"
     ARG             12818..13161
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="98.26"
                     /coverage="40.93"
     Typing          complement(6716..6863)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(6922..7097)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(7229..8211)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8638..9380
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcaatgaca tatagtgata atacagcaaa caataaaatt ataaaagaaa tcggtggaat
       61 caaaaaagtt aaacaacgtc taaaagaact aggagataaa gtaacaaatc cagttagata
      121 tgagatagaa ttaaattact attcaccaaa gagcaaaaaa gatacttcaa cgcctgctgc
      181 tttcggcaag actttaaata aacttatcgc aaatggaaaa ttaagcaaaa aaaataaaaa
      241 tttcttactt gatttaatgt ttaataataa aaacggagac actttaatta aagatggtgt
      301 tccaaaagac tataaggttg ctgataaaag tggtcaagca ataacatatg cttctagaaa
      361 tgatgttgct tttgtttatc ctaagggcca atctgaacct attgttttag tcatttttac
      421 gaataaagac aataaaagtg ataagccaaa tgataagttg ataagtgaaa ccgccaagag
      481 tgtaatgaag gaattttaat attctaaatg cataataaat actgataaca tcttatagtt
      541 tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa ctgattttcc
      601 ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa atattgtata
      661 tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc aatcgaaaaa
      721 gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta aataattctc
      781 atatatcaag caaagtgaca atagcatttt acaagtattt tttattgttc gattagggta
      841 aacctaaata atacataaat tatattacaa tatgtttatt atagaggcta aagtatctta
      901 gaaatttgga tagtaggttt ttcgggaata aaagtatagt tggctttaat atataattaa
      961 gctaagctat catttatatt tactcgatta taattcgcac ttcaactttt ttataacgta
     1021 aaaattaaaa cgtagtttta tttatcttta gtaatatctt tttaaacact acagtttctt
     1081 cattagttaa attagcgaag taatgattat taagttgata ggctttcttt tctaatgttg
     1141 gccataagtc ttttgcttta tttgtactgt ttattagcac agttcttcta ttttcattat
     1201 cagtttgacg tgttattaga tcctttttgg acatcttatc taatattcca gatattgttg
     1261 aaggttctaa atttaaagta ttaccaatcg ctttaggtgt attcaaatta ctattaatca
     1321 agcaattcaa aacaccatat tctgctggag taatattata gggacttaat aaacttttaa
     1381 aatgtttaaa aactctattc tgagaagtac tcaacaaata atttatacaa ttatctaatt
     1441 ccacaactaa aacctctctt aaatcattag tattgactcg cttatcatat catgataagt
     1501 tttgtaggaa aatagttttg caacaaaaac atacttctac aaaacaattg tgaaagaagg
     1561 ctaataaatg ttcaatgcgg tagtagtgag aaaaagtgca tcaaatgtta gttataaagt
     1621 agaaaaagtt gatgaatctc atttatcgaa gggtgatgta ttagtaaaag tggtatactc
     1681 ttcaataaat tataaggata tgctagcttt acaatataaa ggcggtgtaa ttagagatta
     1741 tcctatgatt cctggtatag attttgcagg tattgttgaa tcaagttcga atgataagtt
     1801 taaagaaggc gataatgtat tagttactgg ttatgatgtt ggcgtgacac atacaggtgg
     1861 attttcagaa tatgcacaag tgcctggaga gtggattgtt cctattcccc aagaaatgtc
     1921 tattaaagag tctatggtat atggcacagc cggttttacg gcagcattat caatttttga
     1981 actagaaaaa tatggaatga atatcaaatc acaacctgaa attttagtgt ctggggctac
     2041 aggtggtgtg ggcagtgttg ctatacaaat tttacataaa attggatatg aaaatatcac
     2101 tgctatcgta agaaaagaaa agcaaataga aatcgcaaaa aaattaggtg caaaacaagt
     2161 aataataatt gaagatgata ataaaccgct taaaaaaggt atttttgatt atttcttaga
     2221 tacagtgggg agtaatgtaa cattatacgg tttaaaacgt ctaaattatc aaggtgttgc
     2281 tactgtatgt ggaaatgtag caggaaattc acttaacgct aatatattac cgtttattct
     2341 tagaggtatt aaattaattg gcattgattc tgtatatgtt gatcataata tccgtgaaga
     2401 tatatggagt aagttagcaa atgaatggaa tataggaaat tcacttctat acaatgaaat
     2461 tggatttgaa gaattctata aaacgataga tcagctttta aaaggacaac atttaggaag
     2521 aactatttta aaagtataag tgtgagtatt cattaataca tttactatta attcaatcat
     2581 tttgcatttc tctactaacc agtgttataa tttatataac gaaaatgatt aacaattaat
     2641 gcaccaatcc ccacactatt gcagtagtgt ggggattttt tcgatgctgg cttataaagt
     2701 cgcctattaa ttatttgttg cgtttactaa gccaataagt ataatacgca acagcacagc
     2761 cactgatgac tggtgctatg atgtgaacga aaatgattag caaagtaatg cacctcctct
     2821 cttcgtcaat tgacgcctga gagataggcg actctattat tataccatct gtgaatatac
     2881 aagcatatct acttccgata agtcgtctta tgttatataa ctttgttttt tatagtatca
     2941 agacaagaag aaactcgttt caaaaaccct catgattttt agagaaaaac tctcttattt
     3001 catcgtcttt aataaaataa tatgccatct ttccatcttt ataaaaatca agtacctcat
     3061 ttttatataa aagccttaaa tgatgtgaag ttgaagcaac actcattttc aatatcaaag
     3121 aaatatcaca aacacacaac tcatcttctt taatcaaaga taatatgatt ttcaatttct
     3181 tctcatcaca aattttttct aaaatgttaa gtaatttctt agatttatca tcttctaaaa
     3241 aacttaaagc attgttaact ttatcctcat gtacacagat cacatcacaa gtattttcat
     3301 aactcatata aaatcacacc ctaaattaaa atataaatcc taaaattgtt tgaatagtgt
     3361 cattttcaat aataataaat aaacctaaag ctatataaat aatagccata atccaacgac
     3421 taaatttctc aacaatttct ccaattcctg gaatattagc taatttttgt gcagtaaaca
     3481 ccaagaagaa aattaaaatt aaaaagacaa acagagtaag taataaatta gtaacactta
     3541 atgtcacaaa atacggaaca aataaaccaa tattatcggc gccacaactt gctatcgtaa
     3601 caattgcaac cgtaccaact aatttagaca atcctttttc attcaattct tttttagctc
     3661 tcttttctcc ttcacaatca tcataaatag ccactttaat tccaagataa attggtatta
     3721 aacccaataa acctaatatc cacttttctg gaacataatt taatacaaaa gctaaaaaca
     3781 aactaactaa tattaaaata atagacccta aatattgacc aacataaata tctctatatt
     3841 cttttctagt ctttgctcta gcaaaaaata ttaataaaat tactaataaa tcaactgctg
     3901 tagcaatata aagaacagca gcagcaacaa ccgtttgaat cataatacac ctcattcaaa
     3961 aatatttttg aatgtattat aacttatatt tttaaataat cataggtgct ttattccaat
     4021 tgctttattg acgttgagcc tcggaaccct taacaatccc aaaacttgtc gaatggtcgg
     4081 cttaatagct cacgctatgc cgacattcgt ctgcaagttt agttaagggt tcttctcaac
     4141 atcaataaat tttttcggca taaatgcgtg tctatgcgta gacatggaga cacttcagaa
     4201 ttatggagtg attacatgag tttaactatt tttctttatt cagttttagg aagtttatta
     4261 gcaaattata tctacgaaaa atcttcaaaa taaataactt tttcggttct actttgtaca
     4321 ctttatctac ctggtagacc taattgttat tgctttacag gtctaaaatt cgtcgttata
     4381 atctagttaa atcccgtaag ggcgcactta tacgtcggat aaatcgacat gtgcaaaaac
     4441 gtaatcgacc ttcgtcgatt tttttatttc aaaattaaag aaacggcttg agatattccg
     4501 agcagttata aaaattaatt tgcgttgaat attgctacta cattcttgaa aaagtgatgt
     4561 agatttatag cccataactt ttttgaatta catcgacgat acggcgtcga aagaaaaata
     4621 aatagaccgt atgaaagaaa aagctacctc actttatctg gggtagcttt tattttttat
     4681 gcccttattt tgaattttaa gagggcattt taaagattta ggggtgattc atatagtttt
     4741 atatctaaaa cttatatatg cttttaaaac gcaaatatga gccaaataaa tatatgctat
     4801 tttcaaaaac aaaaatttga gcaaaaacaa taactatttt tagacactgc ccatttacat
     4861 gcaaattaaa aattagcata aaattcttta aaacttcccc acgtttttct aaattcctgt
     4921 agggcgtttt tatcaaaaat caagacaaga agaaactcgt tcacactcgt ttcaaaaaaa
     4981 caagaaatct aatctataca cctaccgttt tccaaccccc tcaaagcata caaattaaaa
     5041 acaaaattat tcataatctt actttctttt aacaaaaaca taaaaaccat aaatagacaa
     5101 attaactatc aaaggcaata aacagtataa aaaaaccacg aataaatctt catcaacatc
     5161 taaaataaaa aagaataaaa caattaacaa aatactaatt aacaaaccta aaagtggcaa
     5221 actttttata tatttttcta aattttcatt cataaaatag actcctttca aaaatatgtt
     5281 tataaatata ttaaaataaa tattgatttt ataaagcgct tacttataca attaaaatat
     5341 cttattatct tggaggtatc aaatgaaaat tataaaatta ttttcaataa tttccttgtt
     5401 cctactggta tttagcgcga atttttctaa tgcttacgca aatgaagaac aaaaaagttc
     5461 tttactagaa aatcaaaaag aaaaattgaa aagcaaaaac atcgaagtat cacaaatgac
     5521 caagaaagaa ataagttttc tagaacaatt gcaaaataaa aatgaagata tgcaaaaatt
     5581 cgaaaagcaa atgaaaaaag aaggatttaa aaaaataaag ctagaagaca aaaattctaa
     5641 attaaaatat ctagctacag aagcaaatga taaaaatgca tatggctaca tagctaataa
     5701 cgcttataaa aatgaaaaaa caaaagaaat tgtagtttct gaaacaatat atgataacta
     5761 caacaaaaaa atcacaaaat tcattgccga aaaaagaagt atgactgaaa acaaagaggg
     5821 aaaaatactt attaacaaaa attacgttga atataaggct gaaaaagttg ataaaaacag
     5881 accggtaaca tatgcaggat tcaaatggaa cggcaaagca ttcgcttgta gtatggctgg
     5941 actttatgca tgtgctcact actgtggagt atgggcaatc gtaaatccaa tagccggcgg
     6001 tgtatgtgaa gcagtttgcg gaactgcatt tgcagctgga tgctctgtag gttgatttta
     6061 ttccaattgt tttattgacg ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa
     6121 tggtcggctt aatagctcac gctatgccga cattcgtctg caagtttagt taagggttct
     6181 tctcaacatc aataaatttt ttcggcataa atacgatgtt ccttatttat cctcataaaa
     6241 aacaatagct gaaatatata accaattact gtgtgtttga atttctaata catgaatatc
     6301 attctttaaa aacctattaa cttgtttatc taattgacga tgagtaaaag tagcgaaagt
     6361 tttaatcttc atgatatctc tccttttata aaaatagcaa tccttattac aatattaaac
     6421 acttttattt tggattttaa gaggcatttt gaagacttag aggcaaatca tataggtttt
     6481 gtacttaaaa atctatacag tctcttaaaa cgcaaatatg agccaaataa atatatgcta
     6541 atttcacaaa caaaaatttc agcaaaatca gtgactaatt ttttagatac tgcccattta
     6601 catgcaaatt taaaaattag cataaaatac tttaaaactt ccccatgttt ttctaaattc
     6661 ctgcagggct tttcttatca aaaacttaga aattttaatt taactctaaa atctccgtaa
     6721 gagcataagg gaaatttgct atcattcaaa aatgatagat ctggcaaggc cagacattcc
     6781 aagggtttta agttatttta aagtgatatc atacaaagct gtagtgtcac acttttgtga
     6841 cactacatac agcatttgtc acaaaaataa gacagcactt ttttacagaa aaacaataaa
     6901 aaaagacatc agttatcact aatgtcttaa ataataattt tgcttttcga aattccaaat
     6961 tgcggtaatt gaatgtgctt tttttcttcg tctgtcgttt tatcttcatc acttgtatta
     7021 atcaaattac catcttcggc atcgtctaag tttaaaattt tatgcttttg tttaagcaat
     7081 ccaccataac ttaacatacg ttttcgatac aaaccttttt ctaaatcact tacaatttct
     7141 tgattttttt cgtcgtcatc agttaaaaag tcagatgatt taacggaata ttttgatgtc
     7201 tcttcgagtg ttataaaatc atttgaatta ttgattaaag atattatatt gtttcatgtt
     7261 tttacggcgc atataaaggg catgtgtttc ttcaagttca gttagaattg tttgcattaa
     7321 ataagcttgc attgacttga ttgtttcctt ttggccttta actcccctat catgtatagc
     7381 ttttttaagt ctttttaaag aagttaatat ttcatcctca atgtcctcta acatgtaatg
     7441 catatcttca tgccttgaat taaatgattt ttttgcttta agaataacag cctttatgat
     7501 ttctaaatct ctataatcat attttaataa ataattagtt agttgttctg ggaattcttg
     7561 caatagcatt tctttatcat caaaattatg tgtaaattga tttgaatgat ttgaatgatt
     7621 actaatttca tttttgtact ttatatcatt caagtcattc atatcattat tctcagtatc
     7681 attaaagtca atgtcattaa agtcagtatc attagtgttc gattttcgaa cttctggacg
     7741 ttcgattttc gaacttctgg acgttcgatt ttcgaacttc tggacgttcg attttcgaac
     7801 ttccttctcg ccgtaaggtt caacatcgtt ttcttctttt tctattttgt aaatatcacg
     7861 ttctgtgaca ataggtttca ttaaatatag aatatttggt ttatttaaac cacgtcgttt
     7921 ttgaatgagt agaccatcgt tttctaaccc tttttttatt tttgttaact tttctttttt
     7981 acaatttaag atttccatta gcttttcatt tgaataaaca aagtaaatgt taccatcttc
     8041 atcaacccag ctatttttaa tagataaatt taagcgatca cgtaagatag cataggctat
     8101 tttcatatca ttagttagat ttttataatt ttcactggta aagaatactt tagggatttg
     8161 ataaaatctt tctttgtaat tttcttgcac tgtaaaatat tgattttgca tgacaaaaaa
     8221 cctctttcca tattttagtt tggtaagagg taataaaacg tttttattta gctttacatt
     8281 tatttaatga tttaggtgct tgcactaaga tattattcat tatataatac aagtatcgaa
     8341 aattatataa ataaacagtt tctaaacgtt ttattaccaa accccgacta ttaccagtag
     8401 ttggggtttt tgttatatta agtttattat agcactttaa ataaacttat aaaagggaaa
     8461 atatagcttt atcaagggtt tataaaccct tgataaatat cataaacctt tcataaaccc
     8521 ttgataaaaa ggttgatgga tgtttatttt tatttattaa tcactttatt attaagcatt
     8581 ataagcttat aatattttat caagggttta taaacatttt ataaagggtt gatgatatga
     8641 aaagtgtgaa aaagttgagt gaagaattag gggttagtaa gcaaacgatt tttaataata
     8701 ttaagcgatt gaatatagag acaataaaac aggaaaacac ttcgtttatt aaagaagata
     8761 ccgatataga aaaaatcatt caaagagtaa atgaaaataa aaagaaatat ggttttgaaa
     8821 gtactactga agacaaacaa aaaaaagggt cggataatat taattttgaa tcgaaaagcg
     8881 atgctcaaat agtagaaatc cttaaaaatc agattgatac tttaaataat caaattgaaa
     8941 aacaagaaag tcgtcatgaa acaacaattg aattttacag aaaagagttg caagaaagat
     9001 caaaattgct tgaaaatcag caagttctgg cattagagag taataaaaag attcaaaaat
     9061 tagaaaatca attagaaaaa gaaaaacacc ttaattatcc ctttgataca tctactaaca
     9121 ctagacaaaa cgttgatgca caagaaaaaa catatactac taatcctgta aatattaaca
     9181 gagatcagga agaaactaaa gaaacagaga ttcaatataa ggatatatct ggaagtcaaa
     9241 gcgacgaaag tacacaaggt gaagaagcaa aaaaagaaga cgttccagct aatctaaatg
     9301 acaatgatag tgatatagaa gaaaagagtg aagaaacgga acctaaaaag gggttttgga
     9361 gtcgtttgtt tggtagctaa aataattgat taggcactaa aagttataaa attttagtgt
     9421 cttttatata gtgagaactt ctggtaatca tcgattatgt aaactaaagt atttcaaata
     9481 attaactgta ttaatactct catcatgatt gcgttataaa cgttcattag tgaaacaggt
     9541 attatattaa aaaagcgtac gaatttgtac gcttttttct tgtgttatta ttaaaatctc
     9601 ataacaggaa aatattatga gattttagac gctgtcagaa cgtctctttt acatcgagtg
     9661 acattgaatc aaagtctttg tatgttaatg atatataaaa ttatttagta gattcattat
     9721 agtaagctag attagttgct ctacttgttt ttattctctt ttgcctctaa tttattgaga
     9781 taacgataaa tagttgtacg tgaaacttgc cactgctctg caattgtttt aattggcgta
     9841 ccattatcat aaagtgtctt aagtaagttt aaatcttgtt tatttaattt ttcaggtcta
     9901 cctccatatc ggcctctagc acgtgctgca attcgaccgg ctgatgatcg ctctaaaatt
     9961 aaattacgtt caaactctgc aaatgctgcg aataaatgaa ataataattg tcctgtagaa
    10021 cttgatttat ccatcgttat attttcttct aaactatgaa aactcacacc tcgttcgtta
    10081 agttcattaa ctaacgtgat taaatcctcc atgttccgtc ctagtctatc taatctccaa
    10141 acaacaatcg tatctcctga cctcgcaaat tcaattgctt tatctaaacc aggtcgctta
    10201 ctttttgaac cactcatatg gtcattaaat atcttttcac aaccatatgt atttaatcga
    10261 tcctcctgta aattcaaatt ttgtaatcca gttgaaactc ttgcataacc tactttcaaa
    10321 atacagacct cctatataca cctttagtgt accataactt gattacgagg tcttttgtga
    10381 acatagattt tttgttgggt ttttgaacac ataattctat gtaatactta cttatttaga
    10441 caaaaaacaa gagtgtacag aaaatggtta ttttctggac actctcatct ttaataatat
    10501 attttaaatg tcattaacaa aactaattta ataagagtca agtatggttt acatatgtat
    10561 tatgtttgaa acatacctct aattactttt tactaatatc atttaaaatg tctcgcaatt
    10621 cttcaatttc tttgttattt aattcttcat ttttcgcaaa attcagcact aaacttttca
    10681 tatcccctcc atacagttta ttaagaaagg ttttagcagt tttcatttta atatcgtctt
    10741 ctttaatatt tgatgagtaa aaataaatat tttctgattt gtatcgtttt ataatctctt
    10801 ttttatatag tcttgtgatt aatgttctaa tcgttttatc actaacttct ttatattttt
    10861 gaatttcaac tacaatttca ttagctgata ctgatttttt atcccatatt atattcataa
    10921 catcccattc agccatagat atttcaactt gcttattggt catttaaaac acccattcct
    10981 tttaatattt tttcacttat taattcagca tttttcccag acgggtttcc atctgataaa
    11041 tgtgtagcaa aataatactt atcatgattt gtaattacgt aacctacaaa ccacccatta
    11101 ttatacttcc cgtttactat acctgtacct gttttcccat acagttcata cttttcattt
    11161 ttcttaatca ataatgaaga agataattga ttctttgctt ttttactaaa atggttattt
    11221 tgttccatca tatttttaaa aactattact tgttcaagat tagatatttt caaactatct
    11281 tccatccaat agcttttata acttcccaaa ttttcattac cataatttaa ttgcttgagt
    11341 tgagccgcag tatagttctt tggtatttga ttactaatac gttcgaagta ccaattaact
    11401 gaattttgca ttgctgtatt taaatcttgt tccttattcc aagactcgaa aagataatgt
    11461 ttatgattcc atgacatacg tgaattttta tcacttataa tatgacggtc aagtccaaac
    11521 agtgctaaat aaattttgta agttgaatca ggagaatatc tttttctact ttctttttca
    11581 ttataaatat agtatttgtc tttcttcatg ctgtacataa cgaaagatcc actatttgaa
    11641 ccaaaatttt tactttcatc taaaatttgg taatcgcttt gtaagggctt tttataatta
    11701 taatcagtta aggattgccc cattaaaaac tggctttgaa ttatcatgat aaaaaaagta
    11761 aaaatacaaa taaaaattag aattaatttt gactgctttt tcagatcggc ttcttttata
    11821 ttaattaatc tacttttgag taatgatttt ttaccattaa atgaatggct taatatattt
    11881 ttgttggaaa atggggtttt taatacggag tccataatag attccgcata agttttaaat
    11941 tcatttttgt taatattatt aaggacaaat ctatcggctt ctacttcatt gtcatgaatt
    12001 atctttcttt ttacaatatg tactaatgga ttgtaactca taataataga gaagatatta
    12061 aagataatta aatgtaaagt atctctattt ttagcatgag catattcatg cagtattata
    12121 tattttagtc ttttatcaat tacactttta aaatatgaac taggaatcaa aataatatat
    12181 ttcccatacc aaaaagttat tggagattga atagcctctg cttttcgaat cacaatattt
    12241 tttttatatt gatggttgaa aagtatcgta tctattttat ttttttcatt ttcgtttaga
    12301 taaagtgact gtttctttaa atatttaaga tataataagg ctttcaaaaa tttaaaactt
    12361 aaaataataa ctaaaactat ccaaataact gtgcagatat tatcaattga atcccaatta
    12421 aacttatgga tatctgttgt gaactcttga ataggtttgg tggtatttat gttatggttc
    12481 aaatcgtgtg acttactttc aactgtgggc gcttgattat tcacattatt aaattttata
    12541 aaagagaatt taattgggat aaaaggaatt aatccagcaa gcatagttag ataccaaact
    12601 ttataattta aactataatt aaaatagcgt tttaatatgt acctaaaaaa tactaacaat
    12661 agaaatataa aacaaaagct tactacgctc attattagta atttagccat tttgacacct
    12721 tctttcaaat atttataata aacaattgac atcaatatta caattgtaat attattgatt
    12781 tataaaaatt acaactgtaa tatcggaggg tttattttga aaaagttaat atttttaatt
    12841 gtaattgctt tagttttaag tgcatgtaat tcaaacagtt cacatgccaa agagttaaat
    12901 gatttagaaa aaaaatataa tgctcatatt ggtgtttatg ctttaaatac taaaagtggt
    12961 aaggaagtaa aatttaattc agataagaga tttgcctatg cttcgacttc aaaagcgata
    13021 aatagtgcta ttttgttaga acaagtacct tataataagt taaataaaaa agtacatatt
    13081 aacaaagatg atatagttgc ttattctcct attttagaaa aatatgtagg aaaagatatc
    13141 actttaaaag aacttattga ggc
//
