LOCUS       CP048294                2461 bp    DNA     circular BCT 07-FEB-2022
DEFINITION  Escherichia coli strain CVM N18EC0432 plasmid pN18EC0432-6, complete
            sequence.
ACCESSION   CP048294
VERSION     CP048294.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN12359680
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Tyson,G.H., Li,C., Hsu,C.-H., Ayers,S., Borenstein,S., Mukherjee,S.,
            Tran,T.-T., McDermott,P. and Zhao,S.
  TITLE     mcr-9 does not confer colistin resistance in NARMS isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2019
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 921x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/03/2020 14:12:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,340
            CDSs (total)                      :: 5,222
            Genes (coding)                    :: 5,030
            CDSs (with protein)               :: 5,030
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 83 of 192
            Pseudo Genes (incomplete)         :: 112 of 192
            Pseudo Genes (internal stop)      :: 37 of 192
            Pseudo Genes (multiple problems)  :: 39 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CVM N18EC0432"
                     /isolation_source="Chicken Wings"
                     /db_xref="taxon:562"
                     /plasmid="pN18EC0432-6"
                     /geo_loc_name="USA:CA-UC Davis"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            794..1339
                     /locus_tag="GZ082_25415"
     CDS             794..1339
                     /locus_tag="GZ082_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002030412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJH52481.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMIN
                     QSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTNND
                     LVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVFNGD
                     RVAFTTVLEMNKSSHKDK"
                     /name="hypothetical protein"
     gene            complement(1419..1844)
                     /locus_tag="GZ082_25420"
     CDS             complement(1419..1844)
                     /locus_tag="GZ082_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJH52482.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
                     /name="mobilization protein"
     Typing          complement(129..388)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.695"
                     /coverage="100.0"
                     /evalue="6.76e-89"
ORIGIN
        1 ccggaaacgc gcctcaaatc caccaagccg ccacggttcc ggctgcgcct tgagcgcaac
       61 cggaaaccga ctggggagaa ccccagactc ccaccgctgc aacatcatcg gtctgcgacc
      121 acctaccaac cgagcggctg cctgtaccgt gcaaaggggc tttgcagggg ctggcgcggt
      181 tcctagcgac ctgaaaaccc ctggttaggg gtatcggcgc gcctggcctc ctgaaaccct
      241 ctgtaagcgt ttttcgcgcc ttccatgccc tggcgggcat ttaaaacccg tttcgtgcgt
      301 ctgagagctt ctgagagcgt tttaggggct atctggcaac tcatccccga aagagcaacc
      361 gcggaatcac cgacaacatg cgagaggtaa ttccgctcgc cgcaagtcga tgaccgagcg
      421 tagcgagcga atcgacgagg aagcggaaga gaaccggaag ctacattgag cacttacgca
      481 cagaatttgg cctacgcccc cgcgtccgtg cacgcgttta ataagaatta agaagggaaa
      541 tcgcgataaa ccgcgccaat cctagaatct aatgatcagg atattgatct aacgtatcag
      601 aatagagcat ctaacatatc aattagttga tatttaaagt attgattgtt tgttttttgc
      661 tcatcgcgag tgtgatttat ttgatcttta aggtatcaaa actatgatac tagtcggatc
      721 ggtttattga catttgtaga ttttttcttg atagtcgagg tatcagttta ttgatacctg
      781 agagtgtaaa ataatgagca agagcaaggc tttaaacata aacaaacgat ttagtgaaaa
      841 tcctttcatc aaagatttga taattcctgt aaaaaacaaa caagtaagaa tatcgcgatt
      901 aggagaagat aataacataa tgattaacca atcaactgga gaggttttag ggggaacctc
      961 ggtagtaact tataaaaaag ttgatagtga taattttatt aaattgttta cccaaaatat
     1021 aaaattggct ttcgagttaa cacaagctgg tcaaaaagca ctatttgttt tattttgggc
     1081 agtacaaaat aaaacaaata atgatctagt cttattagat caatacacgc taaatgattt
     1141 tataaatgaa aacaaaggat taagcatgtc ccttccaaca ttcaaacgtg ggcttgggga
     1201 gttggtaaaa tcacaaatat tagcaatgaa taagcgaaag ggattttatt atataaatcc
     1261 taattttgtt tttaatggcg atagagttgc attcacaaca gttcttgaaa tgaataaatc
     1321 gagccataaa gacaaatgac aatgtattaa gcccgtcaaa ggaaaatttc aaatctattg
     1381 gcgggtttta atattcgtaa cggctagcgc cgaagatttt agctaaatat atttgagcca
     1441 gggtatttat ttttatgatg ccgttttatt atttttattt cctgttttaa tagatccatt
     1501 tcattatata catcagaaat tcgcttcgtt actattttca tttcatcgca aagtaaaatt
     1561 attttatgca tcacctcgag gtactcctta tccttcggcc gctttccctt atcaaataat
     1621 ttcttctgca tccgaccaag tcggattaat tcatctaaat acctggaagg tacgcgctgt
     1681 tttatttcat tatttaaact tgccgtcctg atatattctg aaattgtcag tccttcgttc
     1741 ttcgctcgcc gtctgatttc gcttgcaact ctgttattta ctctaagggt gataacctta
     1801 gttctctgcc gtacttctga accactacgt ttatttttag ccatcgcaat gactctccat
     1861 tttgcacgtt tggggtgtcg gggcttgccc tgacaggagg tattctcatg caagcgaatg
     1921 tattcgcggt atgacgaata cacatcctgt cccattttcc gctacaactt ttcgacgaaa
     1981 ataaaatacc gcgtcgtaac ctggtatttc tgatacaaaa aaacgatgct tcattcaata
     2041 ttatttacac agattttaaa agttattgtt tgttatttaa taaataacgc aaaatcaatt
     2101 gaaacttttg ttctttgtcg tttgttgatt attgagtaaa taaagatcgt gtcttcctga
     2161 ttatataatc cattgcctgt tagtgatacg tagtgtgtcc aaggaacagg aagcggaaga
     2221 gcaagatgta gcatgtgcat aaaatataaa tatgtgagtt attgatatcg catttatata
     2281 ttaaaaatat aacttaacgt cgctcttaat atttatttca tcctggcaat gggagcatta
     2341 atcccgtgca gcgctccgcc agctgctgac ggcctcgaca tgcaaccttc aatccgataa
     2401 aagatcgggg cgcgtttccg gcaacaacta accataggga tggtttgcgt gccgacaaac
     2461 g
//
