LOCUS       CP043755               11451 bp    DNA     circular BCT 15-SEP-2019
DEFINITION  Escherichia coli strain CVM N56338 plasmid pN56338-1, complete
            sequence.
ACCESSION   CP043755
VERSION     CP043755.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN12698088
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Tyson,G.H., Li,C., Hsu,C.-H., Sonya Bodeis-Jones,S. and McDermott,P.
  TITLE     Diverse Fluoroquinolone Resistance Plasmids from Retail Meat E. coli
            in the United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2019) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2019
            Assembly Method                   :: Overlpa-Layout-Consensus v. 4
            Assembly Name                     :: HGAP
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 788x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/09/2019 23:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.9
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,734
            CDSs (total)                      :: 4,615
            Genes (coding)                    :: 4,499
            CDSs (with protein)               :: 4,499
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 61 of 116
            Pseudo Genes (internal stop)      :: 29 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CVM N56338"
                     /isolation_source="Pork Chops"
                     /db_xref="taxon:562"
                     /plasmid="pN56338-1"
                     /geo_loc_name="USA:GA"
                     /collection_date="2015"
                     /collected_by="FDA"
     gene            join(11321..11451,1..90)
                     /locus_tag="F1L17_23570"
                     /pseudo=""
     CDS             join(11321..11451,1..90)
                     /locus_tag="F1L17_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(43..159)
                     /locus_tag="F1L17_23575"
                     /pseudo=""
     CDS             complement(43..159)
                     /locus_tag="F1L17_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557753.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            complement(1214..1702)
                     /locus_tag="F1L17_23580"
     CDS             complement(1214..1702)
                     /locus_tag="F1L17_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QEP68102.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGLV
                     SYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDA
                     LQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            1872..2261
                     /locus_tag="F1L17_23585"
     CDS             1872..2261
                     /locus_tag="F1L17_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QEP68103.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2305..3798
                     /locus_tag="F1L17_23590"
     CDS             2305..3798
                     /locus_tag="F1L17_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="QEP68104.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            3829..4068
                     /locus_tag="F1L17_23595"
     CDS             3829..4068
                     /locus_tag="F1L17_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QEP68105.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTSSAAAVTKTLR"
                     /name="hypothetical protein"
     gene            4252..5598
                     /locus_tag="F1L17_23600"
     CDS             4252..5598
                     /locus_tag="F1L17_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="QEP68112.1"
                     /translation="MSVLQFFIAVDVTVVNVALPSIGDDFGVDPRGLTWVVVGYTITGG
                     GLLMLGGRLGDVFGRRRLLLIGTAIFGAASMMAGFAETFAVLVAARLFQGVGEALALPA
                     AMATIVLLFPEGRARTRALSVWAAVASCGLVLGFVLSGIITEHLGWRWVFLIAVPFILF
                     VLIATVILLPGDRKQERAPLDILGSILLTGAPLLFAFGIVEAGEGMSWLPVAALTGAFI
                     AAILFVGVERRAPNPLIPLAFFRNRTRVRANLATALLSAALSTSFLLFTFYLQDELGLS
                     PLEAGLLLLPLAVALIVAVTVVPRLMGRWGARSCIIAGLGFAGAGMIVIAVAAQLDAPA
                     WVLIPAMLFIAAGMGFGLVGLQYAAVTGVTDDDAGVASGVQRAADQLGGSAGVTVFVGL
                     GFASIAHGVSPYLLSSGLAIIGLGVAAFVARRVAVSDEHTTEQGVNTEA"
                     /name="MFS transporter"
     gene            5620..6564
                     /locus_tag="F1L17_23605"
     CDS             5620..6564
                     /locus_tag="F1L17_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QEP68106.1"
                     /translation="MNYSMVTSGKSPERVESWLRCEPIIARTATIHQPMAPAAYDCVKL
                     VVVRDGSALLLSEFGQRFVTVGDVVVLNANVLCGSEPESHITTTTIYLDTDYVIDQVFW
                     QHAWLLHDRLDAERFTNAVYSEPSQVLRLGEDRTGMLMPWLDELVARSIDGGFGSHFHR
                     MQALWFSIADVVMPFLKVTQIRQSRGQRAHARPTLPRCRRFAPARDEAHQTRATLVSDI
                     ATNWTLDVLSARVNLSPKQLSRVFTETYGKTPLAYLTMMRVEEMAHLLRETHLTIDAAA
                     RQVGWASRSRANEAFQQCVGMTPSEYRRMHRAE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            6802..7734
                     /locus_tag="F1L17_23610"
                     /pseudo=""
     CDS             6802..7734
                     /locus_tag="F1L17_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347778.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /name="ImmA/IrrE family metallo-endopeptidase"
     gene            7770..9137
                     /locus_tag="F1L17_23615"
     CDS             7770..9137
                     /locus_tag="F1L17_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001945043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="QEP68107.1"
                     /translation="MAEQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAF
                     SCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVL
                     GIVYRVIATHLVKKAGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGS
                     ARFRWVKAPTSPELTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLG
                     HSITYRIAVGSQAGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERL
                     CRYISRPAVSEKRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNL
                     TRFHGVFAPNSRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIE
                     TCSGCGGAMKVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91 family transposase"
     gene            9168..9419
                     /locus_tag="F1L17_23620"
     CDS             9168..9419
                     /locus_tag="F1L17_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QEP68108.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTLSAAGQKGIRPCPSR"
                     /name="hypothetical protein"
     gene            complement(9313..9615)
                     /locus_tag="F1L17_23625"
     CDS             complement(9313..9615)
                     /locus_tag="F1L17_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="QEP68109.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHRV
                     VIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
                     /name="phosphoglucosamine mutase"
     gene            complement(9702..10517)
                     /gene="sul2"
                     /locus_tag="F1L17_23630"
     ARG             complement(9706..10517)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10977..11135)
                     /locus_tag="F1L17_23635"
     CDS             complement(10977..11135)
                     /locus_tag="F1L17_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QEP68111.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="replication initiation protein"
     Typing          47..220
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(10969..11451)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.816"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gccggcaccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
       61 gaatatatcc tgtactacat atcctgctga cgcgctggtg ccgccttttt tctcctgcta
      121 catgaagcac ttcactgatt tccacatccg tgccaacata gtcagccagt atacactccg
      181 ctagcgctac gtgactggtt cagggctgcg ccccgaaacc cgctaacaac ctctctgcta
      241 ctggatatca ccccatgcca tctgaatatg atgaacaacg gggttcgact gatacgcagc
      301 ttcccggcga atatgacctt tttgatttaa tggcgaaggt tactgaagag atcctgcatg
      361 cccctgtgga ttctgggccg cctgtcggcc gggaagcgtt atagcttttt cgcttgcccg
      421 cgtacgtggt aatacgccgg tattaccttt tatttcttta ttttcatgta attacagtgg
      481 gtttttaata cctgggtatt actcgtgaaa acgaaacatt actttttttt cttttaattc
      541 atgcagttgc gatgaaaatc gcggctgcgt taggtgtatg accattttga ggttgaaaaa
      601 taatcaacca tggcgggtga tttacgcagg ttaaggggag atcgtgacaa aaaaaagcgt
      661 tcctcactgt atcgttcatg caggcatgaa cgggttattc ttcctgacta cagaattaca
      721 tcgctccttc agttcataca tcacttttac aacactttta caacactcct tcggcctgta
      781 catcactttt acaacacttt tgcatcactt ttacatcatt tatacatcac ttttacatca
      841 ttccttcagc tcatacgtca ctttcatcct ctgtgccatt tgtacatcac ccccttcctg
      901 aacgtgctga cagcgggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat
      961 tgcgtccccg ttcaccgttt tcggatggga agaacgccgg aaacaggact tcccctggct
     1021 gaatggtgtg aatttccgcg ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca
     1081 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg
     1141 tcagaaacgc cctgagggcc tctcaggcgg tgctgataac agatgttatg gtaaatcaga
     1201 gtcacctgca ctgttactct gaaccggagc gaaacagccg cgaccagaat ccccgctttt
     1261 tttcctgtgc cagcgcgtca gccagacggc taatttcggc ctgtagttgt tccctctcct
     1321 catttcgcag gcggttttct gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag
     1381 tctgctgcag agcgtccagt ttctgctgaa tgacgtctag ctgagcattt tccgcctggc
     1441 taacgacaga tttggagtgt ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct
     1501 cgccataggc ccgaatcagt tcactggtct cgaactcccg gcgatcattt tttcctgtgc
     1561 gatagctgac aaggccttta gccatgtcac gatataattg cgagcgcgac tttcctgtta
     1621 atttcagtgt ctcttgaact gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta
     1681 ccgtattgta acagcagaac atattactgt tttaacacaa aaggtctgag gctgcccgtg
     1741 aggcgtgaaa aatacacaaa aaacctgttg acggtgtggt tttgacgggc gtagtatttc
     1801 gaatactgta tataaacaca ttgttataaa tacagttttt gctgttttta aaagtcaaag
     1861 aggataattt tatgagtaca gagttaaata gcggtactgt cggtggtttt ggtcacatgg
     1921 gaccaaatga cccctacaat gatggtcata gcggctctgg aagttcggga ggtagtttta
     1981 acggtggtgg taatggtggc catgataata gtagtggttc atcttatact gcatcctttg
     2041 gtctcatcac tggccgacac ccgataactg tcccaggtac gaagaatatt aattctgtcg
     2101 atctttctgc tgtcattaat aacattatac atcatgaagg tgcacgcaat aacatgtacc
     2161 tagatactgc gaacaatgta agagtgggag tgacgggcac tggctggcaa tgtctagcaa
     2221 cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa
     2281 cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc cagccgggat
     2341 cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta tcagatcgtt
     2401 gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga attgccgggc
     2461 tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca tggctttcta
     2521 cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagctg taagcgtcgc
     2581 ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat
     2641 gaagtactgc ctgaacaacc catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt
     2701 ttcctgtttg ccagccggcc cgagatcatg gggtgggtgc tgggcatcgt ttaccgcgtc
     2761 attgccacgc acctggtcaa gaaagcgggc catacccacc aagtggccaa gacgggcgcg
     2821 gtcaccctga tccagcgttt tggatcggcg ctcaatctga atgttcactt ccacatgctg
     2881 tttctcgacg gtgtgtatgt cgagcaatcc cacggctcag cgcgtttccg ctgggtcaag
     2941 gcgccgacca gcccagagct cacccagctg acgcacacca tcgcccaccg ggtgggtcgc
     3001 tatctggaac ggcaaggcct gctggaacgg gatgtcgaaa acagctatct ggcctcggat
     3061 gcggtggatg acgacccgat gacacccctg ctggggcact cgatcactta ccgtatcgct
     3121 gtcggttcac aggcggggcg aaaggtgttc actttgcaaa ctctgccgac cagtggtgat
     3181 ccgttcggtg acgggattgg caaggtagcc gggtccagcc tgcacgccgg cgtggcggcc
     3241 agggccgatg aacgcaagaa gctcgaacgg ctgtgccggt acatcagccg cccggcggta
     3301 tccgagaagc ggctgtcgtt aacacgaggc ggcaacgtgc gctaccagct caagacgccg
     3361 taccgggacg gcaccacgca cgtcattttc gaaccattgg atttcattgc aaggctggcc
     3421 gccctggtac cgaagcccag agtcaaccta acccgcttcc acggggtgtt cgcacccaac
     3481 agtcggcacc gggcgttggt cacgccggca aaacggggca ggggcaacaa ggtcagggtg
     3541 gctgatgaac cggcaacacc agcacaacgg cgagcgtcga tgacatgggc gcaacggctc
     3601 aagcgtgttt tcaatatcga catcgagacc tgcagcggct gcggcggcgc catgaaagtc
     3661 atcgcctgca ttgaagaccc tatagtgatc aagcagatcc ttgatcacct gaagcacaaa
     3721 gccgaaacca gcgggaccag ggcgttaccc gaaagccggg cgccaccggc tgagctgctc
     3781 ctgggtctgt ttgactgacg agcctgaagg ccaacgatac caatcaaaat gctgcgttca
     3841 cagcgccgcg gcagggatcc gccgtgctgg ttgtcggaaa aggagccgct agtgggaaag
     3901 aggagggtaa attttcagcg ttgctggctc cccgtcagcc ggattgggtt gcatcgcagg
     3961 ggtgtcgaaa gagtcaactg cggtccaaag ctgttggact tgggtgaaaa gggcgtttat
     4021 tcttcctata cgtcgagcgc ggccgctgtg acgaagacac tccgctagcg gcggaacgtc
     4081 tttctcacta cttagtaaac tcacgttccg tcgcttgatg gtcggttcgg tgcgacatac
     4141 cttgtcgccc gattcttcat tcaagcaacg gagttatctg tgtctttttt tacctcaaca
     4201 tcgcgcccgg agcacatctc gaccacgcga tcctgggcag cactcgtgtg cctgtcagtc
     4261 ctgcaatttt tcatcgccgt tgatgttact gtcgtcaacg tagcactgcc atctatcggc
     4321 gatgattttg gcgttgatcc gcgcggcctg acttgggttg tcgtcggata caccatcacc
     4381 ggcggtggcc tactcatgct cggtggccgc ttgggggacg tctttggtcg acgccgcctt
     4441 ctcctgatcg gcaccgcgat tttcggagca gcctcgatga tggcaggttt cgcggaaacc
     4501 ttcgcggtgc ttgttgcagc ccggctgttt caaggagtag gcgaagccct cgccctacca
     4561 gctgcaatgg caaccatcgt cctccttttt cccgagggac gggcccgtac tcgagcgttg
     4621 agcgtctggg cagcggtggc aagctgcggt ctcgtcttgg gattcgtgct ctcgggaatc
     4681 atcaccgagc atctcggttg gcgatgggtc ttcctcatcg ctgtcccgtt catcctgttc
     4741 gtgctgatcg cgacggtaat cctgcttcca ggcgaccgaa aacaggagcg cgcaccgttg
     4801 gatatcctcg ggtcgattct gttgacaggc gcaccgctgc tgttcgcctt cgggattgtg
     4861 gaggccggag aaggaatgtc ttggctgcca gtcgcggccc tgacgggagc attcatcgcg
     4921 gccattctgt tcgtcggtgt agagcggcga gcaccgaacc cgctgattcc tctggccttc
     4981 ttccgcaacc ggactcgggt gcgagcgaat ctcgcgaccg cgttgctgag tgccgcgctc
     5041 tcaacgtcgt tcctactatt cacgttctat cttcaggatg aactcggcct ttccccgctg
     5101 gaagccggac tactgcttct cccgcttgct gtggcgctga tcgtcgcggt gaccgtcgtg
     5161 cctcggctga tggggcgctg gggagcacgc tcctgcatca tcgcgggtct cggcttcgcg
     5221 ggggcaggca tgatcgtgat cgctgtcgcc gcacaactcg atgcccccgc ttgggtattg
     5281 atcccggcga tgcttttcat cgccgcaggt atgggattcg gactagtcgg attgcagtat
     5341 gcggcagtca ccggagtaac tgacgacgat gccggagtcg cttctggtgt acagcgggcc
     5401 gcagaccagc ttggcggttc tgctggcgtc acggtatttg ttggtttggg gtttgcatca
     5461 atcgcccacg gagtatcgcc ctatctattg agtagtgggc ttgcgatcat cggcctcggc
     5521 gtcgcagcct ttgttgcccg acgagttgct gtctccgatg aacacacgac agagcaagga
     5581 gtgaacacgg aagcttagaa agcccagcca gacggactga tgaattactc gatggtgacg
     5641 tcaggcaagt cgccagagag ggttgaatca tggttgcggt gcgaaccgat tattgcgcgc
     5701 accgcaacca ttcaccaacc tatggctccg gcggcgtacg actgcgtcaa gttggtcgtc
     5761 gtccgcgacg gcagcgcgtt gctcttgagc gagttcgggc agcggttcgt taccgtcggt
     5821 gatgtcgttg tgctgaacgc caacgtacta tgcgggagcg aaccggagag tcacatcacc
     5881 acaaccacga tctacctcga cactgactat gtgatcgatc aggtgttctg gcagcacgct
     5941 tggctgctcc atgatcgcct tgatgccgaa cgctttacca acgcggtata cagcgaaccc
     6001 tcacaagtgc tccggctcgg tgaagaccga accggcatgt tgatgccatg gctggatgaa
     6061 ctcgttgcgc gcagcatcga tggtggcttc gggtctcatt ttcatcggat gcaggccctt
     6121 tggttttcga tcgccgatgt ggtgatgccg ttccttaaag tcacgcagat acgtcagagt
     6181 cgcggccagc gcgcgcatgc ccggcctaca ctgcccagat gccgacggtt cgcaccggcc
     6241 agggatgagg cccaccaaac ccgcgcgact ctggtcagtg acatcgccac gaactggacg
     6301 ctggatgtct tgtcagctcg ggtgaattta tccccgaagc aactttcacg agtgttcacc
     6361 gagacttacg gtaagacgcc gttggcatat ttgacgatga tgcgcgtcga ggagatggcg
     6421 cacctgctgc gtgaaactca cttgacgatc gacgcggctg cccgccaggt tggttgggcg
     6481 agccggagcc gtgccaatga ggcatttcaa cagtgtgtgg gtatgacgcc gagcgaatat
     6541 cgccggatgc accgcgccga gtaagcgatc gggaacggga catctctggt gtccttcggg
     6601 acgtcttctt cgacaccgct acctggtgct ggtttttggc tggtaagacg gtaaggactg
     6661 tcgggttcag tattgttcct ggcggtcgtc ccgtactttg acttccgctc gtcgctcatg
     6721 tgttcccggc gtacctggta gtcgcaggcc ctgctcacag cagtggctgg tgactacagg
     6781 tttctgggag aggcggacgc gatggcgaag aagtgggagg aagcacgagt agcgcgtgag
     6841 gcgaagctcg atgggttgca ggagcggttg gtcggcgctg ttgatggcct cgtgactggt
     6901 gaggattggc agcgcgcgat gaccttcgcg gcgaggtttc ggtcgcggtc gttcaacaac
     6961 acattgctga tctggtccca gcatctcgac gcattcgata gcggtcgcgt atccgttgcg
     7021 gagccctcct atgtggcggg gtttcgacag tggcaaacgc tgggccgtca ggtggataag
     7081 gggcagtcgg ggtacatgat cttcgccccg gtcactgccc ggttcgcttc ggcaacccct
     7141 gccgatgcgg cgtcatggcg cagactgcag cgcggcgaga aaccccgggc gggtgaggtc
     7201 gcgcggtcga agatcatcgg ggtgaaaccc gcttacgtgt gggatgtctc gcaaacctca
     7261 ggcaatccga ttcccgaacg cccgggtccg gtgctgttgg aaggcgaggc ccccgatgga
     7321 ctctgggaag gcctcgcaac ccagatcgat gcgcgcggct tcaccgtggt gcaggtgccg
     7381 catgaaggca tgatccacgg cgcgaacggg ctcaccgact acggtgcgaa cactgtcaca
     7441 gtgcgggaaa acatgtcacc tgccgcgcag gtgaagacgc tcacgcatga gcttgcgcat
     7501 gtgatgcttc acggaacgaa taatcctgat gcatctgggc atcgcggggt tggtgaggtg
     7561 gaggccgaat cggttgccct catcgtgggt gctgcgcatg gaatggacac cactggttac
     7621 acgatcccgt acgtgaccgg ttgggccagc accgtgaagg actcatcccc ggtcgaggtc
     7681 gtacaggctg ccggtgaacg ggcgcggaaa accgcgaccg agattctcga tcagccgttg
     7741 acgagtatta cccggcattc gctgcgctta tggcagagca gggaaaggaa ttgccgggct
     7801 atgtgcaacg ggaatttgaa gaatttctcc aatgcgggcg gctggagcat ggctttctac
     7861 gggttcgctg cgagtcttgc cacgccgagc acctggtcgc tttcagctgt aagcgtcgcg
     7921 gtttctgccc gagctgtggg gcgcggcgga tggccgaaag tgccgccttg ctggttgatg
     7981 aagtactgcc tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt
     8041 tcctgtttgc cagccggccc gagatcatgg ggtgggtgct gggcatcgtt taccgcgtca
     8101 ttgccacgca cctggtcaag aaagcgggcc atacccacca agtggccaag acgggcgcgg
     8161 tcaccctgat ccagcgtttt ggatcggcgc tcaatctgaa tgttcacttc cacatgctgt
     8221 ttctcgacgg tgtgtatgtc gagcaatccc acggctcagc gcgtttccgc tgggtcaagg
     8281 cgccgaccag cccagagctc acccagctga cgcacaccat cgcccaccgg gtgggtcgct
     8341 atctggaacg gcaaggcctg ctggaacggg atgtcgaaaa cagctatctg gcctcggatg
     8401 cggtggatga cgacccgatg acacccctgc tggggcactc gatcacttac cgtatcgctg
     8461 tcggttcaca ggcggggcga aaggtgttca ctttgcaaac tctgccgacc agtggtgatc
     8521 cgttcggtga cgggattggc aaggtagccg ggtccagcct gcacgccggc gtggcggcca
     8581 gggccgatga acgcaagaag ctcgaacggc tgtgccggta catcagccgc ccggcggtat
     8641 ccgagaagcg gctgtcgtta acacgaggcg gcaacgtgcg ctaccagctc aagacgccgt
     8701 accgggacgg caccacgcac gtcattttcg aaccattgga tttcattgca aggctggccg
     8761 ccctggtacc gaagcccaga gtcaacctaa cccgcttcca cggggtgttc gcacccaaca
     8821 gtcggcaccg ggcgttggtc acgccggcaa aacggggcag gggcaacaag gtcagggtgg
     8881 ctgatgaacc ggcaacacca gcacaacggc gagcgtcgat gacatgggcg caacggctca
     8941 agcgtgtttt caatatcgac atcgagacct gcagcggctg cggcggcgcc atgaaagtca
     9001 tcgcctgcat tgaagaccct atagtgatca agcagatcct tgatcacctg aagcacaaag
     9061 ccgaaaccag cgggaccagg gcgttacccg aaagccgggc gccaccggct gagctgctcc
     9121 tgggtctgtt tgactgacga gcctgaaggc caacgatacc aatcaaaatg ctgcgttcac
     9181 agcgccgcgg cagggatccg ccgtgctggt tgtcggaaaa ggagccgcta gtgggaaaga
     9241 ggagggtaaa ttttcagcgt tgctggctcc ccgtcagccg gattgggttg catcgcaggg
     9301 gtgtcgaaag agtcaactgc ggtccaaagc tgttggactt gggtgaaaag ggcgtttatt
     9361 cttcctatac gttgtcggca gcgggccaaa aaggaatacg tccatgccca tcgaggtgaa
     9421 accggctgtg agcgcgggtt caagcatata gcccgacagg cgcgtatcct tgccgatcac
     9481 gacacgatgg cggtggtcac cgcgacgaaa gacacggcca gccgccatgc cgacgcgcaa
     9541 ggcggtttcc gccgtcatcg cgccttcgtt ggctttgcca cgaataccgt ctgtgccgaa
     9601 atatttgcgc accataaggt cgattatcct gtcgtcgggt cgccctcaaa ggggacatgc
     9661 ctgctgaacc gcgaatatag agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt
     9721 tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg
     9781 atgaagtcag ctccacctgc ggcggcggca agctctgcag cgagtgtcgc ggccccgaca
     9841 tcccccggac cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc
     9901 accggcaaat cgaagcgcag ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt
     9961 tcgggagcag cccccagaaa aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg
    10021 ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg
    10081 atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt cttgcaccga atgcataacg
    10141 acgagtttgg cagatgattt cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga
    10201 atatcattga gataggccac accacgcgac aaggcatagg cttgcgtcgc gggttgataa
    10261 ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc
    10321 gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg
    10381 tcgatcacat ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc
    10441 gccagatacc ggcctccatc ggagaaactg tccgaggtta tgttgacgat gccgaaaatg
    10501 atgagcgatt tattcatggg ggcttctata ataataataa tcgagcatga gtctcatacg
    10561 gatgctcggg tcgaaaggga atccccaggc gagtaacctg tttgcggtga tccgtttcat
    10621 gatgatgtat ctgtagcatt ttggaaataa atatatcatc tctcaataag tagatatatt
    10681 tatctttttt gcttctttgg tgctgcaagt caccaatacg agggaaacct gttacggagc
    10741 ggtttaaccg taatcaacag tgactggtgt catgttcgga agattaattg atagtaatct
    10801 acaaaggatc ttcttgagat ccttttcaat gaatcgtaat ctcttgctct gtaaacgaaa
    10861 aaaccgcctg gggaggcggt ttgttcgaag gttaagtcag ttggggaact gcttaacccg
    10921 gtaacagggt tcacgagacc gccattacca aaactgttct ttcagtgtag ccgcagtcag
    10981 gcctctactt caagacctct taatctatct tctcgtgcac tcctgttact aatggctgct
    11041 gccagtggcg ttttgtcgtg tctttccggg ttggactcaa gttgatagtt accggacaag
    11101 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
    11161 ttcacggaac tgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggaatgaca
    11221 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc
    11281 tgggatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattttgcga
    11341 tgcttgtcag gggggcggag cctatggaaa aacggcttcg ccgtggcctt atctcttctt
    11401 tgctaagtgt cttcctggta tcttccagga aatttccgtc cccgtccgta a
//
