LOCUS       CP043368                5596 bp    DNA     circular BCT 14-MAR-2022
DEFINITION  Klebsiella pneumoniae strain WCHKP090045 plasmid p2_090045, complete
            sequence.
ACCESSION   CP043368
VERSION     CP043368.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN09842413
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wei,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP090045
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wei,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/27/2019 13:19:54
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 5,647
            CDSs (total)                      :: 5,525
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 57 of 152
            Pseudo Genes (incomplete)         :: 94 of 152
            Pseudo Genes (internal stop)      :: 26 of 152
            Pseudo Genes (multiple problems)  :: 22 of 152
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP090045"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_090045"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017"
     gene            complement(join(5559..5596,1..342))
                     /locus_tag="D0S90_28195"
                     /pseudo=""
     CDS             complement(join(5559..5596,1..342))
                     /locus_tag="D0S90_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1030..1260
                     /locus_tag="D0S90_28200"
                     /pseudo=""
     CDS             1030..1260
                     /locus_tag="D0S90_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1611..1955
                     /locus_tag="D0S90_28205"
     CDS             1611..1955
                     /locus_tag="D0S90_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UNC74175.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1894..2658
                     /locus_tag="D0S90_28210"
                     /pseudo=""
     CDS             1894..2658
                     /locus_tag="D0S90_28210"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilisation nuclease domain-containing
                     protein"
                     /name="relaxase/mobilisation nuclease domain-containing
                     protein"
     gene            2633..3142
                     /locus_tag="D0S90_28215"
     CDS             2633..3142
                     /locus_tag="D0S90_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UNC74176.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="mobilization protein"
     gene            3149..3361
                     /gene="mbeD"
                     /locus_tag="D0S90_28220"
     CDS             3149..3361
                     /gene="mbeD"
                     /locus_tag="D0S90_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UNC74177.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="mbeD"
     gene            4028..4252
                     /locus_tag="D0S90_28225"
     CDS             4028..4252
                     /locus_tag="D0S90_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="UNC74178.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            4236..4502
                     /locus_tag="D0S90_28230"
     CDS             4236..4502
                     /locus_tag="D0S90_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UNC74179.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4572..4922)
                     /locus_tag="D0S90_28235"
     CDS             complement(4572..4922)
                     /locus_tag="D0S90_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UNC74180.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4988..5533)
                     /locus_tag="D0S90_28240"
     CDS             complement(4988..5533)
                     /locus_tag="D0S90_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UNC74181.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          1280..1359
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(697..1094)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1946..3477
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctttagaac catcatgtca ttaataattg aatctatctc atccattttt tcttggtatt
       61 cttttaatgg tgaagggagc ggtaactcaa tatttgaaaa gtgattggcg agatagggat
      121 caaggtttct catccattca ggcttttcgc caatgcgttc ttcaactgaa agtgaacctg
      181 ataatctctg gttaataaga tcacttagac tcattttggt tttattcgaa agccgttgta
      241 aggattgctt taatcctata tctagcatga tatttgttct ttctctcatt accccccctt
      301 gctattgcgt tttttgttgc gcacaaagct accattgcgc attaagaaac gcaataaaaa
      361 aggagggagg gagtggtttg tacatgatgt gcagtgcgct gagggcatca gtggcgggtg
      421 tttttccgtg ggatggatgg agctccgggg ataacagaac tgacgatcat ttaaaggatc
      481 ttcttgagat cctttttttc tgcgcgtaat ctcttgcact gtaaacgaaa aaaccgcctg
      541 gggaggcggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgg gtaactgggt
      601 atacgagacc gcagatacca aaactgttct ttcagtgtag ccgcagttag gcacccactt
      661 caagacctct caatatctct ctcgtatata tccagttacc aatggctgct gccagtggcg
      721 tttttgcgtg tccgtccggg ttggactcaa gatgatagtt accggaaagg gcgcagcagt
      781 cgggctgaac ggggggttcg tgcatacagc ccagcttgga gcgaacgacc taaaccgagc
      841 cgagatacca gcagcgtgag ctatgagaaa gcgccacgct tcccgaaagg gaaaaaggcg
      901 gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcacgaggg agccgccgga
      961 gagaaatcac cggcatcttt aagtcctgtc gggtttcgtc ctcctctgac ttgagcgtcc
     1021 atttctgtga tgctcgtcag gggaggcgga gcctatggaa aaacggcaac gccgcatcct
     1081 tttctccggc cctttctgat ttgagcgcct ttgctttatc ccgttatccg tgagtcggtc
     1141 acgcccgccg cagccgaacg gcaggagcgc agctaccgag tgagcgagga ggcgtcattt
     1201 catctgtccg tgcatggtat tgcggacgct cctgagccac atatcacacc tgccacatga
     1261 ggcactttca tgaatcgtgc tctcatgcca acatagtaag ccagtataca cagcgtaaac
     1321 gctgtgactg gttcagggct gcgccccgaa acccgctaac accactgacg cgctggcgct
     1381 tgtccaacgc cggagcgacc cggaaaactt ttccgtgccg tccggcgttt gcgaaggagg
     1441 gataatgcag aagccgaaga agaagccgct cgtcgtaaaa aaaagatcct ggcatccggc
     1501 agtgatggca aatctgatgc gtcaggcaca ggaggaagat taccgccggg agcagatggc
     1561 agagcttgag cgttcagccc ggtcacattt tgcggatatc ggaggagagg atggcggtta
     1621 agcgtacccg ttttttaggt atccgggtga ccgatgggga gtaccagcag ctgctggagc
     1681 gctgcaatgg aaggcaactg gcggtgtgga tgcgggaaac ctgtctcgat acgcgcccgg
     1741 cgcggtcgtt gcggctgccg tccatcgatc cggttttgct gcgccagctt gccgggatgg
     1801 ggaacaacct caaccagatc gcccggaaaa ttaacggcgg ccagtggtca ggcgctgacc
     1861 gggttcaggt ggtggccgcg ctgatggcca ttgatgccga actcgagcgg ctgcggcatg
     1921 ccgtgctgga aaagggggca gacgatgatc gttaagtttc atccgcgagg gcgaggcggc
     1981 ggtgccgggc cggtggatta tctgctgggg aaagaccgcc agcgcgacgg ggccatcgtc
     2041 ctgcagggaa agccggagga ggtccgggag cttatcgacg cctcgcccta cgccaaaaag
     2101 tacacctccg gcgtcctgtc ttttgctgaa caggatttac cgcccggcca gcgtgaaaag
     2161 ctgatggcga gcttcgagcg ggttctgatg cccggactcg ataaagacca gtacagcgtg
     2221 ctgtgggttg aacaccggga caaggggcgg ctggaactga attttctggt accgaacacg
     2281 gaactgctga ccggcaggcg tctccagccg tactacgacc gggcagaccg tccgcgcatc
     2341 gatgcgtggc agaccgtggt gaacgggcga ctggggctgc acgacccgaa cgcaccggag
     2401 aaccggcgcg cgctggtgac gccatcttcg ttacccaaaa cgaagcagga agccgcagag
     2461 gctattacgc ggggcttact ggccctcgcc tcgtcagggg agcttaaaac gcgtcaggac
     2521 gtcactgagg cgctggaaag cgcaggtttt gaggtcgtgc gcaccacaaa gagcagcatc
     2581 agcattgccg acccggacgg agggcgaaac atccgactta agggagccat ctatgaacag
     2641 tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc agagtaccgg
     2701 cgaaatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg aactgagcga
     2761 aagcgggaag agaatcagcg ccgccatccg cgcccacgag cagggtatga gcgcggccat
     2821 gcagtcgaac cgcctgagcg tgatgcgtat ggtcgggcgg acatggctga ccatcacgat
     2881 ggtgtcagga ctgctgttcg ccagcctgag cggggtgttg tggtatcagg ggagcttgat
     2941 agcgtcaaat ctggccgaaa tcgaccggca gaacgcagcg ctgtcgaagc tgaacgcgaa
     3001 gacctggggc gtgacgtacc tggaagacag caacggacgt tttctggtgc tgccgaaggg
     3061 gacggccgta gacaggacgc agagctggac ggtcgggaac gggcggtcga agcagaacgc
     3121 gctgagactg gtgaaggagt aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc
     3181 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga gcgccttcgg
     3241 ggaatggcgg aaaatgtcag ggcttatgca acgggagaac gcggcgctga gcgagcgcgt
     3301 gacgcgcttg agtcagcagg tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta
     3361 gtgcagcgtc atgagatggc cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa
     3421 aaggaactga cggaagcgcg ttcccggcag cgaagctatg acggtccgtc gctgggctga
     3481 aatgcaggaa aacagggttc tcatcgggca tttacgctga aaccggatgt cattcacctg
     3541 gctgcatggc tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt
     3601 ggtttgttgc cggttttacc tgactgccac cccgttcgcg gcgaacccgt ccggggcggt
     3661 gcgggcaacg ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa gccccggagg
     3721 gcttttaact actgacttat ttttttgaag atttgctggg tttcaatttg tcagtatttc
     3781 ccggcgtttt tttattgtca cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc
     3841 gtttcggacc cggcttaaga cccataatcg ttatcctttc aatagttaag aggaaagccc
     3901 tcaagctaga tatattgacc gcgttacgga ttatcaagag gggctaacaa gattattttt
     3961 tgttctttta ggttgattgt ggtactgttg atatacatgt atagcaaatg gtgttcggga
     4021 ggtttatatg cttgctatcc ggttatctga tgagattgag tcccgtctgg actcgctggc
     4081 gaagcaaacc ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg cgcatctgga
     4141 agacctggag gattattatc tttcagcaga aactgctgca cgcgttcgcc gtggtgatga
     4201 agcagtgcat tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt aactattccg
     4261 atcgggcgct caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg attgtggatt
     4321 ttatgagttt acgcattgca gttgctgccg atcctcgcca gtcagggaag ccgctcaaag
     4381 gtgagctggg cgagttctgg cgctatcggg tgggagatta tcgcgttctg tgtgagatcc
     4441 gagatgacga gcttgttatc cttgccgcca cgattggaca tcgccgcgaa gtttacgact
     4501 gaagcccgct atgcgggctt ttttgatgcc gacaacaggt attatggtaa atactcgact
     4561 aactacgtgt tctactgttt gtcggtttta gttaaccagc aggttgtctt aacgataatg
     4621 tagccgttat ttttaactaa taaggtcata ttgttaacgt caggcataat aaaagacatt
     4681 atggcctcag agttatcatc agaagcagct ctctgcatta ctacttcgat tcccttttct
     4741 ttgaatctat aagtatccag ttgcctatta aacttagaaa gacctacaac catacttagt
     4801 ttgttatctt taacagtaag agatgcttcc tgagtaccat tacatcggta gtaagcggtg
     4861 taaccgtcag caaaagatcg accggggaat ataaatgcta ggaacgcaat taaccaatac
     4921 atagttgaga atcttggtat tacacatttc actatcctct ctttcatttt tcgttcacca
     4981 tactttgtta tctgattttt aatttttcta ttttataaac caagtctcta ccaaaaaggc
     5041 gaaaattatc ctcaaataat aataattgtt taagaacatc catgaaagca gctgcttcac
     5101 cctcagttat gggagctact ctatataaca tataacctga gttaagggtt tcttttatac
     5161 tttcttcaat gctcattata aatattagtg gattatcatt tttcttcccc catgggtttc
     5221 taattaatcc ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca atatttttta
     5281 tttcatctgc gtgagggctt tgttgtacag ctttgtttat gcctgcttga gtgattttca
     5341 gagactctac accgtgtatg ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa
     5401 ttaatccatt tttgtttaca tctgagtatt tggataaaaa acgtaatgaa gtgttaactc
     5461 tcccttgaag cctaacaatt gggagtgctg ccttgtatat ttccttctcc gttaaatacc
     5521 gtttaaaaaa cataattctc cgcctccgta tagcgtgcct attttatttc gtcgtaaaac
     5581 ttagctctga aatatt
//
