LOCUS       CP042504                2495 bp    DNA     circular BCT 09-OCT-2019
DEFINITION  Enterobacter sp. E76 plasmid pE76_005, complete sequence.
ACCESSION   CP042504
VERSION     CP042504.1
DBLINK      BioProject: PRJNA550014
            BioSample: SAMN12289293
KEYWORDS    .
SOURCE      Enterobacter sp. E76
  ORGANISM  Enterobacter sp. E76
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: E76_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2425x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/02/2019 15:05:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,028
            Genes (coding)                    :: 4,892
            CDSs (with protein)               :: 4,892
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 31 of 136
            Pseudo Genes (incomplete)         :: 94 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 16 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter sp. E76"
                     /mol_type="genomic DNA"
                     /strain="E76"
                     /isolation_source="Shower 3"
                     /db_xref="taxon:2596949"
                     /plasmid="pE76_005"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="07-May-2014"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            complement(join(2353..2495,1..151))
                     /locus_tag="FR762_25685"
     CDS             complement(join(2353..2495,1..151))
                     /locus_tag="FR762_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFH73145.1"
                     /translation="MSVRFCDACQGGGAYGKTALARPYAFFVKYLPGIPQEISDPSVSP
                     SRSPQPNDRAERVSERGSGIYSVSHFLLTRFLSLSAPVLCGINAMCYCHATS"
                     /name="hypothetical protein"
     gene            32..274
                     /locus_tag="FR762_25690"
                     /pseudo=""
     CDS             32..274
                     /locus_tag="FR762_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526573.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            336..419
                     /locus_tag="FR762_25695"
     CDS             336..419
                     /locus_tag="FR762_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QFH73146.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHD"
                     /name="replication initiation protein"
     gene            898..1125
                     /locus_tag="FR762_25700"
     CDS             898..1125
                     /locus_tag="FR762_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QFH73142.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1491..1676
                     /locus_tag="FR762_25705"
     CDS             1491..1676
                     /locus_tag="FR762_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QFH73143.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1678..1862)
                     /locus_tag="FR762_25710"
                     /pseudo=""
     CDS             complement(1678..1862)
                     /locus_tag="FR762_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105484.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1967..2158)
                     /locus_tag="FR762_25715"
     CDS             complement(1967..2158)
                     /locus_tag="FR762_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFH73144.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1724..1811)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..483
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.73"
                     /coverage="88.0"
                     /evalue="1.9600000000000003e-157"
ORIGIN
        1 gctgcggcga gcgggacggg cttacggatg gatcagaaat ttcctggggg atgccaggaa
       61 gatacttaac gaagaaagca taaggccgcg ccaaagccgt ttttccatag gctccgcccc
      121 cctgacaagc atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt
      181 aaagatacca ggcgtttccc cctggtggct ccctcgtgct cccccgcatt cctgcctttc
      241 ggtttaccgg tgtcactccg ctgttatggc cgcggtttct cattccacgc ctgacactca
      301 gttccgggaa ggcagttcgc tccaggatgg actgtatgca cgaacccccc gttcagtccg
      361 accgctgcgc cttatccggt aactatcaac ttgagtccaa cccggaaaga cacgactaat
      421 cgccattggc aacggccatt ggcaaacagg atgtgcgaga gatgtctcaa gagttcttga
      481 agtggcggcc ttagtgaggc tacactgaaa ggacaaattt ggtgacatgt ctcgcacaag
      541 actgttacca cggttagggg atctgccagg attacctaac cttcgaaaaa ccacctgcca
      601 gggtggtttt ttcgtttaca gggcaagaga ttacgcgcgg aaaaaaagga tctcaagaag
      661 atcctttata aattagacat ccaacctcac aaagaggaat acttaaatca tcttggtata
      721 tgtatcggcc ggattacatc tcatataagc ctcctccccg cctgacacca cactttgacc
      781 atagcacctc gcgcctaact cgatatacag cccaccatca agcctctttt cacataaaaa
      841 atgacctata gttagatggt tagtgaccaa aactgatatg gataaaagga gctggctttg
      901 aaaaagattt ttgtttcagt gttcgcagcg gcagttgcgc tttcagccct caccggctgc
      961 acgcgcacaa gctatgccat acacaccaat gacggtcgca ctattgtcag cgacggcaaa
     1021 cctaaagaat cagactcagg cctgcttggc tatacagacg cgaatggtgt caaacagcag
     1081 atcaataaaa ccgacgttaa agaagtctcc gaaattccgc actgagcatc ttctccgaaa
     1141 aaggccgcta atgtggcctt tttctttacc tgtggttctc cgccaaaatc ccagcaaatt
     1201 gcatcaccaa agctaaaagc tttcagggtt gtctaaaaag cacaagacat aagaggaagt
     1261 gcggtatttt ataatcaagc ccccaagaat ttaccataac atccgttgcc cgcaccgcct
     1321 gagacgcgtt cagcgcgttc ctgacgaaac catgacaaaa accacaacaa accaccccgg
     1381 aacccgtcag aaacgcgcct gttaaatttt aacggcatgc atgactatgc accagaatga
     1441 cgccatgctc ttttcacgca aaaatcatca ccagacgggg aaaatcacca gtgaccagac
     1501 aggaatccgc cgccctcaat atggccaaat ttatccgcgc acagacactt ctcctccttg
     1561 agcggctcga gcagatggat ctggatgagg ctgccggctg ctgtgagcac ctgcacgatc
     1621 aggccgaagc gctttacgcc atgctgaacg cacagatagg cgaggaaaat gcgtgaaaat
     1681 cggtgaacgg gtgcgcaatt cagtgcgcgg ccgtgaggcg atggcggggt gtcggggcgc
     1741 agccctgacc agggtatttg tgatgccggc gcgtgcgcgg tattacaaat gcacatcctg
     1801 tcccggaacg gacaccggga aacagcaaaa aaaaccgggc ggcacgcccg gaactcaatc
     1861 aagttagatt agattactct cactcgtcca taacagcatc atggaacgac gaccaccgtc
     1921 cgtgacggcc gcctcgttta agtatggaca gaaatacaga aaatgctcag gacgaaatgt
     1981 aatgaatgcg aacggattca agaaattcga gcatgacagt ccttacggcc ggttcggttt
     2041 cagacaaaat ctgccggtat gcatccagca tcatggctcc ggcatcccct ccggcacgcc
     2101 gtagccagac cgaaacaacg gacacaagca ggtgtcgctc atcatcacta agagtcatca
     2161 gggctccgga agaaaaacca aacatggctt acctactgtt aacggcaacg cagaagaaag
     2221 gtttacaggg tagcgggcag ggatgaaaaa aaaaaaattg cagtcaggcc ggaacggctg
     2281 ctgaaccggt acaggcgggt tccgggcgca gcgccggacc agtcacggag cactagcgga
     2341 gtgtatactg gcttaagatg tagcatggca gtaacacata gcattaatgc cacataagac
     2401 aggcgcagaa agtgaaagaa aacgcgtcag gagaaaatgt gatacagaat atattccgct
     2461 tcctcgctca ctgactcgct ccgctcggtc gttcg
//
