LOCUS       CP042503                3811 bp    DNA     circular BCT 09-OCT-2019
DEFINITION  Enterobacter sp. E76 plasmid pE76_004, complete sequence.
ACCESSION   CP042503
VERSION     CP042503.1
DBLINK      BioProject: PRJNA550014
            BioSample: SAMN12289293
KEYWORDS    .
SOURCE      Enterobacter sp. E76
  ORGANISM  Enterobacter sp. E76
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: E76_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2425x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/02/2019 15:05:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,028
            Genes (coding)                    :: 4,892
            CDSs (with protein)               :: 4,892
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 31 of 136
            Pseudo Genes (incomplete)         :: 94 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 16 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Enterobacter sp. E76"
                     /mol_type="genomic DNA"
                     /strain="E76"
                     /isolation_source="Shower 3"
                     /db_xref="taxon:2596949"
                     /plasmid="pE76_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="07-May-2014"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            join(3750..3811,1..703)
                     /locus_tag="FR762_25645"
                     /pseudo=""
     CDS             join(3750..3811,1..703)
                     /locus_tag="FR762_25645"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            678..1169
                     /locus_tag="FR762_25650"
     CDS             678..1169
                     /locus_tag="FR762_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QFH73136.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="mobilization protein"
     gene            1176..1391
                     /gene="mbeD"
                     /locus_tag="FR762_25655"
     CDS             1176..1391
                     /gene="mbeD"
                     /locus_tag="FR762_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QFH73137.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWQSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="mbeD"
     gene            1992..2333
                     /locus_tag="FR762_25660"
     CDS             1992..2333
                     /locus_tag="FR762_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QFH73138.1"
                     /translation="MAKPPSATPPDTPSSCDVSALPGIIQNFGLINFNVSCNNGELTVT
                     GTDQNGKHIRVNSYQGNGFTEGTMSTFQPGGKEARRLEAVRLRESGLTQQEIANRLGVS
                     QKTISNDLK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2492..2784)
                     /locus_tag="FR762_25665"
                     /pseudo=""
     CDS             complement(2492..2784)
                     /locus_tag="FR762_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2864..3235)
                     /locus_tag="FR762_25670"
     CDS             complement(2864..3235)
                     /locus_tag="FR762_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFH73139.1"
                     /translation="MQYRRYSASSLTDSLRSVVRLRRAVPAYEWGGNFLVDARKELNRE
                     TIRPRQSRFSIGSAPLTSITKSDAQISGGETRQDYKDTRRFPLMAPSCALLFLPFGLPV
                     SLRCYGRVYLIPRLTLGSG"
                     /name="hypothetical protein"
     gene            complement(3198..3314)
                     /locus_tag="FR762_25675"
     CDS             complement(3198..3314)
                     /locus_tag="FR762_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QFH73140.1"
                     /translation="MLAWMRKSVKCFMWQEKKGGTGASTEDAIQEIFRFLAH"
                     /name="plasmid replication initiation protein"
     gene            3467..3811
                     /locus_tag="FR762_25680"
     CDS             3467..3811
                     /locus_tag="FR762_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QFH73141.1"
                     /translation="MSEKRSKMLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPAR
                     TGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3202..3375
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          complement(2727..3235)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          3..1483
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.548"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3489..3808
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.523"
                     /coverage="100.0"
                     /evalue="2.82e-61"
ORIGIN
        1 atttcatcca cggggtcgcg gcggtggcgc cgggccggtg gactatctgc tgggtaaaga
       61 ccggcagcgt gaaggcgcca ccgtcctgca ggggaagccg gaagaagtcc gggagctgat
      121 tgacgcgtcg ccttacgtga agaaatacac ctccggcgtc ctgtcgtttg cggaggccga
      181 tctccctccc ggccagcggg aaaaactgat ggccagcttc gagcgggttc tgatgcccgg
      241 actcgataaa gatcagtaca gcattctgtg ggtggagcac gcggacaagg ggcgtctgga
      301 gctgaacttt ctgatcccca ataccgagct gctgaccggc aggcggctcc agccttacta
      361 cgaccacgcc gaccggccgc gcatcgatgc ctggcagact atcgtgaacg ggagactggg
      421 gctgcacgac ccgaacgcgc cggaaaaccg ccgggcgctg gtcacgccgt ccgggctgcc
      481 gaaaacgacg caggaggccg cagaagccat tacccgggga ctactgagct tagcctcgtc
      541 cggggagctg aaaagccgtc aggacgtcac tgaggctctt gaaggtgcag gttttgaggt
      601 ggtgcgcacc acgaaaagca gtatcagcat tgccgacccg gacggggggc gaaacatccg
      661 actgaaggga gctatctatg agcaatcttt ttacggcctc gagggacttc gaggagaaat
      721 tgaaagcgca gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgagc
      781 agtctgtcag agcggaactg agcgcaagcg cgaggagaat cagcgacgcc atcctcgccc
      841 acgagcagag tatgagcgag gcgatggaga aaaaccggcg gagcgtactg cggactgccg
      901 ggcggacatg gctcaccatc ctgatggtgt cagcgctgct gatcggcacg agcgggagca
      961 ttctgtggtg gcaggggcag cagataaccg acaattacac gcatcttcgt cagcaggaag
     1021 ataccctggc gaaaatgacc gcccggacgt ggggagtgcg ctatcaggag agcagcgacg
     1081 gccggaggtt tcttatcctg ccgccgggga tgcaaaccga agccatccct tacgacggga
     1141 cgacatggat ccgcctgaaa caggagtagc ggaaaatgac agagctggaa aagcagttgc
     1201 tgagcgcatt agagcagcta cagcaggact actcgcaaag gctggacgaa tgggagagcg
     1261 ccttcgcgga atggcagagc atgtgtggtc ttatgcaacg ggagaacgcg atgctgaacg
     1321 agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag cgagcagctg cgccgcttgt
     1381 ccgggagctg aatgccatcg agctccggca cagggagcat gagtatcaga aaacactgga
     1441 gctggagcac cagccgcaga aaacctaccg tggcccaacg ctgggctgac ggcccgtgac
     1501 aaaccccaag aagaagggct cataaatttc ccgctaaata cggtcgtgat ggaaacatca
     1561 cagcgtatac aacggaggga ttatgagact gtttaacgtg attgagacgg tgctgaactg
     1621 cctgtacatg gcgctggaga tctgggactg gctggagaag cactttcctg tttttaaggc
     1681 gttcgcgctg attttagtgt gactttacgc ggcatgcatc tggctgcatg actatgcatc
     1741 agtgtaatat ccagcggctg cgtttctggt gttttttcgt tatgtctgtc gccactttct
     1801 cgcgtctggc accggaaacg cagccaaccc cgtttcagcg gtgcgggcaa cggatgttat
     1861 ggtaaatgta gggggagaag tgactataag caggggcttc ggtgatggcc taaagggata
     1921 tggattattg atattactag ttttctagta tttactagta tggatgcatc gtaaaaatca
     1981 aggaacgcac aatggcaaaa cctccatcag ctacaccacc ggatacacca tcttcatgtg
     2041 atgttagtgc tctcccaggc attatccaaa attttggctt aattaacttt aacgtatcct
     2101 gcaataacgg tgagctaacg gtaacgggta cggatcaaaa tgggaaacac ataagagtta
     2161 acagctacca agggaacggc tttaccgaag gtacaatgtc aacgtttcag cccggaggta
     2221 aagaggccag aagacttgag gctgttcgtt taagagaaag tgggcttacg cagcaagaaa
     2281 tagcaaaccg gttaggcgtt tctcaaaaaa ctataagcaa tgatttaaaa tgagtcactt
     2341 gctttaagcc cgtcttaacg ggcttttcat cgctactgaa atgccatgat aaatccggcg
     2401 tagttgctcg tcccgcagtg gtgaaacacc taaagataca tttcagatgt ttattttaag
     2461 ccatggtgca gatccccata tatgtcgaac atcaataatg atcttcttga gatcctttcc
     2521 attgcatcgt aatctcttgc tctgaaaacg aaaaaaccgc ctggggaggc ggtttttcga
     2581 aggttaagtc agttggggaa ctgcttaacc tggtaactgg cttcagcaga gcgcagatac
     2641 caaatactgt ccttccagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2701 cgcctacata tctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgtttttt
     2761 cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact
     2821 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaaccgagtg
     2881 tcaggcgtgg aatgagataa acgcggccat aacagcggag tgacaccggt aaaccgaaag
     2941 gcaggaacag gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt
     3001 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     3061 cggagcctat ggaaaaacgg ctttgccgcg gccttatcgt ttccctgtta agttccttcc
     3121 tggcatctac caggaaattt ccgccccatt cgtaagccgg taccgctcgc cgcagccgaa
     3181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tctcctgtat tgcatcttct
     3241 gttgacgcgc cggtgccacc ttttttctcc tgccacatga agcacttcac tgatttccgc
     3301 atccatgcca acatagtaag ccagtataca ctccgctagc gctgcgtgac tggttcaggg
     3361 ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg cttgtccaac cccgcgccga
     3421 ccgggaaagg ctttcccgcc cgtcccggcg ttatcaggag gccggtttgt cagagaaacg
     3481 gagcaagatg ctcaccatgt gggtgacgga ggacgagcac cggcggctgc tcgagcgctg
     3541 tgacggcagg cagctggcgg cgtggatgcg gcagatctgc ctggacgaaa agcctgcgcg
     3601 taccggaaag cttccctcaa tctcgccggc gctgctgcgc cagcttgccg gcatgggcaa
     3661 taacctgaac cagatagccc gccgtgttaa cgccggcggc ggtaccggcc atgacagggt
     3721 gcagattgtg gcggcgctga tggccatcga tgccggactt gagcggctgc ggcacgccgt
     3781 tcttgagaaa ggaacggacg atgatcgtta a
//
